Supplemental Table III. A list of potential cis- and trans- acting elements in AnnAt1 promoter.

TF (DNA binding domain) / Family / Prototypical/deduced core sequence / Orientation / Position* / Rel. distance / Max. Score / Threshold / Score
ABF1 / bZIP
(leucine zipper) / ABRE (ABA-responsive element) with G-box:
5’-CACGTGGC-3’ / + / 13226360 / -49 / 13.16 / 10.94 / 13.16
HAHB4 / HD-ZIP (homeobox with leucine zipper) / 5’-CAAT(A/T)ATTG-3’ / + / 13226377 / -32 / 9.64 / 5.32 / 7.40
Opaque-2 (O2) / bZIP
(leucine zipper) / prolamin box from a-zein
5’-GTGTAAAG-3’ / -
+
-
+ / 13226359
13226359
13226416
13226416 / -50
-50
+7
+7 / 10.35 / 4.83 / 8.65
8.38
8.24
8.03
ZmHOX2a(1) / HD-HOX (homeobox with PHD zinc finger, Cys4–His–Cys3, C4HC3) / 5’-TCTT-….-GATC-3’ / +
+
-
- / 13226426
13225964
13226049
13226076 / 17
-445
-360
-333 / 7.33 / 4.29 / 7.33
5.67
5.31
5.31
DOF2 / C2C2(Zn)Dof (DNA binding with one CX2CX21CX2C zinc finger) / 5’-(T/A)AAAG-3’ / +
+
+
+
+
+
+ / 13225929
13225984
13226085
13226089
13226271
13226316
13226344 / -480
-425
-324
-320
-138
-93
-65 / 8.49 / 2.72 / 4.4
3.77
4.00
3.85
3.48
4.33
4.79
AGL2
(Agamous like) / MADS / CArG box:
5’-CC(A/T)6GG-3’ / + / 13226015 / -394 / 13.0 / 6.18 / 8.79
GT1 / Trihelix DNA-binding motif / GT-element:
5’-GPu(T/A)AA(T/A)-3’ / +
-
+ / 13225924
13225958
13226183 / -485
-451
-226 / 8.94 / 2.72 / 6.17
4.21
5.19
TEIL / AP2/EREBP / 5’-A(A/C)G(A/T)A(A/C)CT-3’ / + / 13225974 / -435 / 8.38 / 3.81 / 5.09
TGA1a / bZIP / as-1 type sequence G-box
5’-TGACGTGG-3’ / -
+ / 13226359
13226359 / -50
-50 / 10.45
10.45 / 5.21
5.21 / 7.82
7.44

* chromosome 1, complete sequence (nt)

Analysis of -500 bp DNA upstream of the start translation codon of AnnAt1 (At1g35720) performed with web software www.athamap.de designed for in silico analysis of potential transcription factors binding sites for the whole Arabidopsis genome. The potential regulatory elements with the sequence score above the threshold are indicated.

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