Supplementary Resource Material

SUPPLEMENTARY RESOURCE MATERIAL

Features of a unique intronless cluster of class I small heat shock protein genes in tandem with box C/D snoRNA genes on chromosome 6 in tomato (Solanum lycopersicum)

Ravinder K. Goyal1,2,3, Vinod Kumar1,2, Vijaya Shukla1,2,4, Rohini Mattoo5, Yongsheng Liu6, Sang Ho Chung1,7, James J. Giovannoni6, Autar K. Mattoo 1,*

1The Henry A. Wallace Beltsville Agricultural Research Center, U.S. Department of Agriculture, Agriculture Research Service, Beltsville, MD 20705-2350, USA

4University of Maryland Biotechnology Institute, College Park, MD 20742, USA

5Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India

6USDA-ARS, Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA

2These authors contributed equally to this article

3Present address: Agriculture and Agri-Food Canada-Lethbridge Research Centre, P.O.3000, 5403-1stAve. South,Lethbridge, Alberta, T1J 4B1 Canada

7Present address: Department of Microbiology, Mokwon University, Doan-dong 800, Seo-ku, Taejon 302-729, Republic of Korea

*To whom correspondence should be addressed. E-mail: ; FAX: +13015046491

Supplementary Table 1. List of primers used for indicated analysis.

Primer / Sequence (5’ to 3’) / Experiment
qRTActin-Fwd / TGGTCGTACCACCGGTATTGTG / Q-PCR
qRTActin-Rev / AATGGCATGTGGAAGGGCATAC / Q-PCR
qRT20.1-Fwd / CTTCATTTGCTTGTTAAG / Q-PCR
qRT20.1-Rev / GTATTTCATATCTTGAACATC / Q-PCR
qRT20-Fwd / GTGAATTTATTGTATCCTTG / Q-PCR
qRT20-Rev / TACATCAACTACGCCATTA / Q-PCR
qRT17.6-Fwd / CCAATGAAGCTATAAATAAC / cDNA probe for GF isolation, Q-PCR
qRT17.6-Rev / AGAGACATTTTCAGTGAAC / cDNA probe for GF isolation, Q-PCR
qRTEST1-Fwd / GCAATATTTGAAGTAAAATGTTTGTAA / Q-PCR
qRTEST1-Rev / CGCCTATCTCGCTATATACACA / Q-PCR
qRTU6-Fwd / CGA TAC AGA GAA GAT TAG CAT G / Q-PCR
qRTU6-Rev / AAG CTC CAT TGT CAA ATT TT / Q-PCR
qRTR12-Fwd / TGATGGTGTGTAAGAATGAT / RT-PCR, Q-PCR
qRTR12-Rev / AGTCAGAAGTAGCTGGTTGT / RT-PCR, Q-PCR
qRTU24-Fwd / AGGGCCTGTGATGTAAGTAA / RT-PCR, Q-PCR
qRTU24-Rev / AGAACAAGGCCTCAGAGATC / RT-PCR, Q-PCR
RTZ132-Fwd / AAAGGGCCTGTGATGTAAG / RT-PCR
RTZ132-Rev / GGCCTCAGAGATCTTGGTGG / RT-PCR

Supplementary Table 2. List of protein sequences in NCBI database used for building cladogram for Sl17.6 SHSP and its orthologs (Fig. 4).

Given in sequence are accession numbers, name given and the name of the organism.

4836473 / AF123257_1 17.6 kD class I small heat shock protein / Solanum lycopersicum
4836469 / AF123255_1 17.7 kD class I small heat shock protein / Solanum lycopersicum
300827456 / cytosolic class I small heat shock protein 3B / Nicotiana tabacum
300827454 / cytosolic class I small heat shock protein 3A / Nicotiana tabacum
3341464 / Hsp20.1 protein / Solanum peruvianum
4836471 / AF123256_1 17.8 kD class I small heat shock protein / Solanum lycopersicum
232273 / HSP11_SOLLC RecName: Full=17.8 kDa class I heat shock protein
75279026 / HSP11_SOLPE RecName: Full=17.7 kDa class I heat shock protein; AltName: Full=Hsp19.9
327493231 / class I heat shock protein / Solanum nigrum
75279027 / HSP12_SOLPE RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=Hsp20.0
123538 / HSP13_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-M
255631095 / unknown / Glycine max
255633534 / unknown / Glycine max
87240494 / Heat shock protein Hsp20 / Medicago truncatula
217075124 / unknown / Medicago truncatula
123534 / HSP11_SOYBN RecName: Full=17.3 kDa class I heat shock protein; AltName: Full=HSP 17.3
255633382 / unknown / Glycine max
320090070 / heat shock protein / Gossypium hirsutum
123556 / HSP12_MEDSA RecName: Full=18.2 kDa class I heat shock protein
224132048 / predicted protein / Populus trichocarpa
123544 / HSP16_SOYBN RecName: Full=18.5 kDa class I heat shock protein; AltName: Full=HSP 18.5
255627179 / unknown / Glycine max
224104357 / predicted protein / Populus trichocarpa
123539 / HSP14_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-E
159138937 / low molecular weight heat shock protein / Gossypium hirsutum
224545 / 1107298A protein,small heat shock
83701135 / 17.3 kDa class I heat shock protein / Arachis hypogaea
255626519 / unknown / Glycine max
38639431 / 17.5 kDa class I heat shock protein / Carica papaya
255558882 / heat-shock protein, putative / Ricinus communis
224103037 / predicted protein / Populus trichocarpa
37704437 / cytosolic class I small heat shock protein 3B / Nicotiana tabacum
123540 / HSP15_SOYBN RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=HSP 17.6-L
37704433 / cytosolic class I small heat shock protein 3A / Nicotiana tabacum
123554 / HSP11_MEDSA RecName: Full=18.1 kDa class I heat shock protein
224146364 / predicted protein / Populus trichocarpa
255558918 / heat-shock protein, putative / Ricinus communis
224104214 / predicted protein / Populus trichocarpa
15222395 / class I heat shock protein / Arabidopsis thaliana
189909524 / low molecular weight heat shock protein / Camellia sinensis
314998618 / small heat shock protein / Jatropha curcas
224132044 / predicted protein / Populus trichocarpa
255558916 / heat-shock protein, putative / Ricinus communis
159138945 / low molecular weight heat shock protein / Gossypium hirsutum
284433776 / heat-shock protein / Jatropha curcas
37704445 / cytosolic class I small heat shock protein 3C / Nicotiana tabacum
83776798 / 17.5 kDa class I HSP / Arachis hypogaea
283482284 / cytosolic class I small heat shock protein type 2 / Rhododendron simsii
255558876 / heat-shock protein, putative / Ricinus communis
224132040 / predicted protein / Populus trichocarpa
25809052 / heat shock protein / Pisum sativum
99033693 / chaperone / Agave tequilana
255558920 / heat-shock protein, putative / Ricinus communis
224146037 / predicted protein / Populus trichocarpa
224104209 / predicted protein / Populus trichocarpa
159138935 / low molecular weight heat shock protein / Gossypium hirsutum
18073562 / 17.6 kDa heat-shock protein / Helianthus annuus
117188222 / heat shock protein 17.8 / Rosa chinensis
8671873 / AC018748_15 Contains similarity to 17.6 KD class I heat shock protein from Arabidopsis thaliana gi
159138941 / low molecular weight heat shock protein / Gossypium hirsutum
255558878 / heat-shock protein, putative / Ricinus communis
259123933 / CI small heat shock protein 2 / Prunus salicina
158828220 / HSP21 / Cleome spinosa
99033683 / chaperone / Agave tequilana
195972629 / 17.67 kDa heat-shock protein / Helianthus annuus
161702917 / 17.66 kDa class I small heat shock protein / Ageratina adenophora
15220832 / HSP20-like chaperone / Arabidopsis thaliana
224156134 / predicted protein / Populus trichocarpa
123555 / HSP11_PEA RecName: Full=18.1 kDa class I heat shock protein; AltName: Full=HSP 18.1
224146360 / predicted protein / Populus trichocarpa
255579096 / heat-shock protein, putative / Ricinus communis
224101075 / predicted protein / Populus trichocarpa
21592809 / heat shock protein, putative / Arabidopsis thaliana
154293473 / 17.7 kDa class I small heat shock protein / Vigna unguiculata
224109416 / predicted protein / Populus trichocarpa
41059801 / small heat shock protein / Prunus persica
283482276 / cytosolic class I small heat shock protein type 2 / Rhododendron ovatum
297840571 / hypothetical protein ARALYDRAFT_893564 / Arabidopsis lyrata subsp. lyrata
15227552 / HSP20 family protein / Arabidopsis thaliana
225449270 / PREDICTED: hypothetical protein / Vitis vinifera
147785904 / hypothetical protein VITISV_007166 / Vitis vinifera
283482272 / cytosolic class I small heat shock protein type 2 / Rhododendron nakaharae
116779529 / unknown / Picea sitchensis
40646966 / chloroplast small heat shock protein class I / Capsicum frutescens
255630413 / unknown / Glycine max
169101 / 17.9 kDa heat shock protein (hsp17.9) / Pisum sativum
11990130 / 17.9 kDa heat-shock protein / Helianthus annuus
283482266 / cytosolic class I small heat shock protein type 2 / Rhododendron kanehirai
147805491 / hypothetical protein VITISV_020895 / Vitis vinifera
225449280 / PREDICTED: hypothetical protein / Vitis vinifera
283482262 / cytosolic class I small heat shock protein type 2 / Rhododendron hyperythrum
15218934 / HSP20-like chaperone / Arabidopsis thaliana
147820339 / hypothetical protein VITISV_028926 / Vitis vinifera
147841212 / hypothetical protein VITISV_039648 / Vitis vinifera
16930753 / AF439277_1 small heat shock protein / Retama raetam
224054773 / predicted protein / Populus trichocarpa
99033689 / chaperone / Agave tequilana
283482278 / cytosolic class I small heat shock protein type 2 / Rhododendron pseudochrysanthum
283482270 / cytosolic class I small heat shock protein type 2 / Rhododendron kawakamii
115452113 / Os03g0266300 / Oryza sativa Japonica Group

Supplementary Table 3. List of protein sequences in NCBI database used for building cladogram for Sl20.0 SHSP and its orthologs (Fig. 5).

Complete names of the organisms are given in Suppl. Table 2.

4836473 / AF123257_1 17.6 kD class I small heat shock protein / S. lycopersicum
4836469 / AF123255_1 17.7 kD class I small heat shock protein / S. lycopersicum
300827456 / cytosolic class I small heat shock protein 3B / N. tabacum
300827454 / cytosolic class I small heat shock protein 3A / N. tabacum
3341464 / Hsp20.1 protein / S. peruvianum
4836471 / AF123256_1 17.8 kD class I small heat shock protein / S. lycopersicum
232273 / HSP11_SOLLC RecName: Full=17.8 kDa class I heat shock protein
75279026 / HSP11_SOLPE RecName: Full=17.7 kDa class I heat shock protein; AltName: Full=Hsp19.9
327493231 / class I heat shock protein / S. nigrum
75279027 / HSP12_SOLPE RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=Hsp20.0
123538 / HSP13_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-M
255631095 / unknown / G. max
255633534 / unknown / G. max
87240494 / Heat shock protein Hsp20 / M. truncatula
217075124 / unknown / M. truncatula
123534 / HSP11_SOYBN RecName: Full=17.3 kDa class I heat shock protein; AltName: Full=HSP 17.3
255633382 / unknown / G. max
320090070 / heat shock protein / G. hirsutum
123556 / HSP12_MEDSA RecName: Full=18.2 kDa class I heat shock protein
224132048 / predicted protein / P. trichocarpa
123544 / HSP16_SOYBN RecName: Full=18.5 kDa class I heat shock protein; AltName: Full=HSP 18.5
255627179 / unknown / G. max
224104357 / predicted protein / P. trichocarpa
123539 / HSP14_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-E
159138937 / low molecular weight heat shock protein / G. hirsutum
224545 / 1107298A protein,small heat shock
83701135 / 17.3 kDa class I heat shock protein / A. hypogaea
255626519 / unknown / G. max
38639431 / 17.5 kDa class I heat shock protein / C. papaya
255558882 / heat-shock protein, putative / R. communis
224103037 / predicted protein / P. trichocarpa
37704437 / cytosolic class I small heat shock protein 3B / N. tabacum
123540 / HSP15_SOYBN RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=HSP 17.6-L
37704433 / cytosolic class I small heat shock protein 3A / N. tabacum
123554 / HSP11_MEDSA RecName: Full=18.1 kDa class I heat shock protein
224146364 / predicted protein / P. trichocarpa
255558918 / heat-shock protein, putative / R. communis
224104214 / predicted protein / P. trichocarpa
15222395 / class I heat shock protein / A. thaliana
189909524 / low molecular weight heat shock protein / C. sinensis
314998618 / small heat shock protein / J. curcas
224132044 / predicted protein / P. trichocarpa
255558916 / heat-shock protein, putative / R. communis
159138945 / low molecular weight heat shock protein / G. hirsutum
284433776 / heat-shock protein / J. curcas
37704445 / cytosolic class I small heat shock protein 3C / N. tabacum
83776798 / 17.5 kDa class I HSP / A. hypogaea
283482284 / cytosolic class I small heat shock protein type 2 / R. simsii
255558876 / heat-shock protein, putative / R. communis
224132040 / predicted protein / P. trichocarpa
25809052 / heat shock protein / P. sativum
99033693 / chaperone / A. tequilana
255558920 / heat-shock protein, putative / R. communis
224146037 / predicted protein / P. trichocarpa
224104209 / predicted protein / P. trichocarpa
159138935 / low molecular weight heat shock protein / G. hirsutum
18073562 / 17.6 kDa heat-shock protein / H. annuus
117188222 / heat shock protein 17.8 / R. chinensis
8671873 / AC018748_15 Contains similarity to 17.6 KD class I heat shock protein from Arabidopsis thaliana gi
159138941 / low molecular weight heat shock protein / G. hirsutum
255558878 / heat-shock protein, putative / R. communis
259123933 / CI small heat shock protein 2 / P. salicina
158828220 / HSP21 / C. spinosa
99033683 / chaperone / A. tequilana
195972629 / 17.67 kDa heat-shock protein / H. annuus
161702917 / 17.66 kDa class I small heat shock protein / A. adenophora
15220832 / HSP20-like chaperone / A. thaliana
224156134 / predicted protein / P. trichocarpa
123555 / HSP11_PEA RecName: Full=18.1 kDa class I heat shock protein; AltName: Full=HSP 18.1
224146360 / predicted protein / P. trichocarpa
255579096 / heat-shock protein, putative / R. communis
224101075 / predicted protein / P. trichocarpa
21592809 / heat shock protein, putative / A. thaliana
154293473 / 17.7 kDa class I small heat shock protein / V. unguiculata
224109416 / predicted protein / P. trichocarpa
41059801 / small heat shock protein / P. persica
283482276 / cytosolic class I small heat shock protein type 2 / R. ovatum
297840571 / hypothetical protein ARALYDRAFT_893564 / A. lyrata subsp. lyrata
15227552 / HSP20 family protein / A. thaliana
225449270 / PREDICTED: hypothetical protein / V. vinifera
147785904 / hypothetical protein VITISV_007166 / V. vinifera
283482272 / cytosolic class I small heat shock protein type 2 / R. nakaharae
116779529 / unknown / P. sitchensis
40646966 / chloroplast small heat shock protein class I / C. frutescens
255630413 / unknown / G. max
169101 / 17.9 kDa heat shock protein (hsp17.9) / P. sativum
11990130 / 17.9 kDa heat-shock protein / H. annuus
283482266 / cytosolic class I small heat shock protein type 2 / R. kanehirai
147805491 / hypothetical protein VITISV_020895 / V. vinifera
225449280 / PREDICTED: hypothetical protein / V. vinifera
283482262 / cytosolic class I small heat shock protein type 2 / R. hyperythrum
15218934 / HSP20-like chaperone / A. thaliana
147820339 / hypothetical protein VITISV_028926 / V. vinifera
147841212 / hypothetical protein VITISV_039648 / V. vinifera
16930753 / AF439277_1 small heat shock protein / R. raetam
224054773 / predicted protein / P. trichocarpa
99033689 / chaperone / A. tequilana
283482278 / cytosolic class I small heat shock protein type 2 / R. pseudochrysanthum
283482270 / cytosolic class I small heat shock protein type 2 / R. kawakamii
115452113 / Os03g0266300 / O. sativa Japonica
305678731 / small heat shock protein 20 / Klebsiella pneumoniae
42526539 / Hsp20/alpha crystallin family protein / Treponema denticola ATCC 35405
318041086 / heat shock protein Hsp20 / Synechococcus sp. CB0101
296274629 / heat shock protein Hsp20 / Arcobacter nitrofigilis DSM 7299
325980913 / heat shock protein Hsp20 / Nitrosomonas sp. AL212

Supplementary Table 4. List of protein sequences in NCBI database used for building cladogram for Sl20.1 SHSP and its orthologs (Fig. 6).

Complete names of the organisms are given in Suppl. Table 2.

3341464 / Hsp20.1 protein / S. peruvianum
300827456 / cytosolic class I small heat shock protein 3B / N. tabacum
300827454 / cytosolic class I small heat shock protein 3A / N. tabacum
4836473 / AF123257_1 17.6 kD class I small heat shock protein / S. lycopersicum
75279026 / HSP11_SOLPE RecName: Full=17.7 kDa class I heat shock protein; AltName: Full=Hsp19.9
4836469 / AF123255_1 17.7 kD class I small heat shock protein / S. lycopersicum
4836471 / AF123256_1 17.8 kD class I small heat shock protein / S. lycopersicum
232273 / HSP11_SOLLC RecName: Full=17.8 kDa class I heat shock protein
327493231 / class I heat shock protein / S. nigrum
75279027 / HSP12_SOLPE RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=Hsp20.0
255631095 / unknown / G. max
123538 / HSP13_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-M
255633534 / unknown / G. max
87240494 / Heat shock protein Hsp20 / M. truncatula
123544 / HSP16_SOYBN RecName: Full=18.5 kDa class I heat shock protein; AltName: Full=HSP 18.5
123534 / HSP11_SOYBN RecName: Full=17.3 kDa class I heat shock protein; AltName: Full=HSP 17.3
224132048 / predicted protein / P. trichocarpa
255633382 / unknown / G. max
320090070 / heat shock protein / G. hirsutum
255627179 / unknown / G. max
217075124 / unknown / M. truncatula
159138937 / low molecular weight heat shock protein / G. hirsutum
38639431 / 17.5 kDa class I heat shock protein / C. papaya
123539 / HSP14_SOYBN RecName: Full=17.5 kDa class I heat shock protein; AltName: Full=HSP 17.5-E
123556 / HSP12_MEDSA RecName: Full=18.2 kDa class I heat shock protein
83701135 / 17.3 kDa class I heat shock protein / A. hypogaea
224545 / 1107298A protein,small heat shock
224104357 / predicted protein / P. trichocarpa
255626519 / unknown / G. max
224103037 / predicted protein / P. trichocarpa
255558882 / heat-shock protein, putative / R. communis
224104214 / predicted protein / P. trichocarpa
255558916 / heat-shock protein, putative / R. communis
314998618 / small heat shock protein / J. curcas
123540 / HSP15_SOYBN RecName: Full=17.6 kDa class I heat shock protein; AltName: Full=HSP 17.6-L
37704445 / cytosolic class I small heat shock protein 3C / N. tabacum
37704437 / cytosolic class I small heat shock protein 3B / N. tabacum
284433776 / heat-shock protein / J. curcas
224146364 / predicted protein / P. trichocarpa
37704433 / cytosolic class I small heat shock protein 3A / N. tabacum
255558918 / heat-shock protein, putative / R. communis
224132044 / predicted protein / P. trichocarpa
159138945 / low molecular weight heat shock protein / G. hirsutum
189909524 / low molecular weight heat shock protein / C. sinensis
255558876 / heat-shock protein, putative / R. communis
83776798 / 17.5 kDa class I HSP / A. hypogaea
15222395 / class I heat shock protein / A. thaliana
123554 / HSP11_MEDSA RecName: Full=18.1 kDa class I heat shock protein
283482284 / cytosolic class I small heat shock protein type 2 / R. simsii
255558920 / heat-shock protein, putative / R. communis
224104209 / predicted protein / P. trichocarpa
25809052 / heat shock protein / P. sativum
224132040 / predicted protein / P. trichocarpa
224146037 / predicted protein / P. trichocarpa
159138935 / low molecular weight heat shock protein / G. hirsutum
158828220 / HSP21 / C. spinosa
159138941 / low molecular weight heat shock protein / G. hirsutum
18073562 / 17.6 kDa heat-shock protein / H. annuus
99033693 / chaperone / A. tequilana
195972629 / 17.67 kDa heat-shock protein / H. annuus
255558878 / heat-shock protein, putative / R. communis
224146360 / predicted protein / P. trichocarpa
259123933 / CI small heat shock protein 2 / P. salicina
224156134 / predicted protein / P. trichocarpa
161702917 / 17.66 kDa class I small heat shock protein / A. adenophora
255558872 / heat-shock protein, putative / R. communis
99033683 / chaperone / A. tequilana
15227552 / HSP20 family protein / A. thaliana
117188222 / heat shock protein 17.8 / R. chinensis
123555 / HSP11_PEA RecName: Full=18.1 kDa class I heat shock protein; AltName: Full=HSP 18.1
224101075 / predicted protein / P. trichocarpa
255579096 / heat-shock protein, putative / R. communis
21592809 / heat shock protein, putative / A. thaliana
15220832 / HSP20-like chaperone / A. thaliana
154293473 / 17.7 kDa class I small heat shock protein / V. unguiculata
224109416 / predicted protein / P. trichocarpa
297840571 / hypothetical protein ARALYDRAFT_893564 / A. lyrata subsp. lyrata
283482276 / cytosolic class I small heat shock protein type 2 / R. ovatum
16930753 / AF439277_1 small heat shock protein / R. raetam
41059801 / small heat shock protein / P. persica
283482272 / cytosolic class I small heat shock protein type 2 / R. nakaharae
11990130 / 17.9 kDa heat-shock protein / H. annuus
225449270 / PREDICTED: hypothetical protein / V. vinifera
40646966 / chloroplast small heat shock protein class I / C. frutescens
147785904 / hypothetical protein VITISV_007166 / V. vinifera
15218934 / HSP20-like chaperone / A. thaliana
283482266 / cytosolic class I small heat shock protein type 2 / R. kanehirai
283482262 / cytosolic class I small heat shock protein type 2 / R. hyperythrum
225449280 / PREDICTED: hypothetical protein / V. vinifera
147820339 / hypothetical protein VITISV_028926 / V. vinifera
147841212 / hypothetical protein VITISV_039648 / V. vinifera
147805491 / hypothetical protein VITISV_020895 / V. vinifera
283482278 / cytosolic class I small heat shock protein type 2 / R. pseudochrysanthum
56606538 / heat shock protein 18 / C. lanceolata
82941445 / heat shock protein 18 / C. lanceolata
169101 / 17.9 kDa heat shock protein (hsp17.9) / P. sativum
283482270 / cytosolic class I small heat shock protein type 2 / R. kawakamii
283482282 / cytosolic class I small heat shock protein type 2 / R. rubropunctatum
115452113 / Os03g0266300 / O. sativa Japonica
283482274 / cytosolic class I small heat shock protein type 2 / R. oldhamii
305678731 / small heat shock protein 20 / Klebsiella pneumoniae
42526539 / Hsp20/alpha crystallin family protein / Treponema denticola ATCC 35405
318041086 / heat shock protein Hsp20 / Synechococcus sp. CB0101
296274629 / heat shock protein Hsp20 / Arcobacter nitrofigilis DSM 7299
325980913 / heat shock protein Hsp20 / Nitrosomonas sp. AL212

Supplementary Fig 1. The complete DNA sequence of the isolated genomic fragment from tomato. Full length is 8.304 kb.

1 GTCGACTCGA TCTTTGAACC TGAGTGGGTC AAGTCCGGGC ACAAAGTCTT

51 CGGCAATTGA GTCTGTTGAA ACAGAAAAAA GAACAATGGT TAGTCCAAAA

101 AAAGGCTAGC AATGTATGTA TAAGGTTAAA TTTTTTGTTA TAACACATCA

151 TAATAAACTC TTCTAATGAT GTGTTACAGG ACAAGTGAAT TAAGGATACC

201 TTTTAACGGA AAATAACCTT CAGATTCAAG TCTGTTAAAA GCAGCATAAG

251 TCCAAAAAAG GCTAGCACTG CTAGTTTCCA AAATGATACT AGGAAGCTTA

301 AGATTGTTAG CCACCTCTGC TACTTTATAC ATAATAGAAT CATAAATAAT

351 GCACAAAATC TCATCATTTT GTTGTTGTTG ATGAACCAAT ATATCTCGAA

401 GAGGCATCGC GATGTTATCA TTAAGAGCTA GCAATAATGC TATAAAATTA

451 CAAGAAGAGG CTTGAAATTG AGAGAGATCA TGTGGTATAG GGAGAAAAGA

501 GAAACAAGGA TGATTTGATG GATTTGGAAA GTTAAATTGG GAGTGAGCAA

551 ATGTAATAGA AAAGCCCTTC AAGTAAAGAA GCGAGGCTAA TTCAAACATT

601 GGGTTTATGT GACCTTGAAA TGGACAAGGG ACTAGTACAA TTCTACTTGA

651 TTTTTGTTTT TCCATTTCCA TTTCTCTTTT CAACTTAGAT ATGGTTTTTT

701 TTGTTTAGCT AAAATTGTTA GGACTTTATA CTATCATATG ATCTTTGGTA

751 AGAAATCATG AGTGTGATTT AGTCAAAGAA TTTTAATTTT TATAATGTTA

801 GTGAAAGTTC TTCGGAAACT TCAATACAGT TGAAAGAGGA ATTCAAATTC

851 CATAAAATGA CATATTTTAT TTTGGTTTGA CTTTACCAAA ATAAAATCTT

901 ATTTAACACA TTGATTTTCT TTGTTTTGAT AAACATTAGT TATCATATTT

951 ATTTATAGTG AGAAATTGTC TATTTTCCAA CACCTGTAAT AGTATTTATA

1001 AGGACTAGAT AACAAATTAA TGGGATAATG GACTAACATA CACCCTTATG

1051 AGTGGACCGA TCCAATAGCT ATTTGCAACA CTCTTTTAGA GATTTCACTT

1101 CTTTTTTTTA CAATATGAAA TAAATAGTGA ACTAAGAAAC CTAGTAGCTA

1151 ATTATACATA CATTAAGATA CCACACTTTT AACTATGTTT TAGTATTTAC

1201 GTTTTTTTTT CAATCAAAAT GCTCTCTGAC AGAGACGATT CTATACCCAA

1251 GAAATCTCAA ACCAAAAATT TCTTAATTAA GAATGAAAGA GTAATTACTA

1301 CTCCAACATA ACTTTTGTTT GTTCATTTAC TCACTTTATG GAACCTTAGA

1351 TTTTTCTGAA ATAATTGAGA GTGTTAGGTT ATTTATAAGG NCCAAAATAT

1401 CCTTGTGATT TGCAAAAAGA GTTATCTTTG CATTTCGTCC AAAGTTTATA

1451 TCAATTATAG CCGGTGCTTC ATCGTTACAA AAATAGCACA CATTTACTTT

1501 AATTTTTTGA CTTCCATCTT ATAAAAATGN TGTCCATGAT ATGAGTGGTG

1551 ATCGTGCTAT TTGTGTAATA TTTTAGCCCT CTTCGTAAAA CAAACAAAGT

1601 TAATGGGCCG TTGTAATGAA CAGTCCTTGG GCCAGTGGAA GCCCAATTTC

1651 CATAGCTCAG TTGTAATCTG TAATGGGCCT AATCCAGAAT GTTCTCGACA

1701 TCTGCTTCTG GAACATCTAC TAGGCTCTAG AAATGTCTAA TAGTAGAAGT

1751 GTGGAGAAAG CGCCAAATAC TCCTCTAATG AAGCTATAAA ATAACTACAA

1801 GCTCCTCTTC CATCAACCAT CAAAAATTGA AAAGTAAGCA AACACAGAAA

1851 TTTCTCAAAA AGCTAACCAA AAATGTCTCT GATCCCAAGA ATCTTCGGCG

1901 ACCGACGAAG CAGTAGCATG TTCGATCCAT TTTCAATTGA CGTATTTGAT

1951 CAATTTAGGG GATTAGGCTT TCCAGGTACC AATTCAGGGG AGACCTCTGC

2001 ATTTGCTAAC ACTCGAATCG ATTGGAAGGA AACTCCAGAA GCTCATGTGT

2051 TCAAGGCTGA TCTTCCAGGG CTTAAGAAGG AGGAAGTGAA AGTGGAGATC

2101 GAAGAAGATA GGGTTCTTCA GATCAGCGGA GAGAGGAACG TGGAGAAAGA

2151 AGATAAGAAC GACACTTGGC ATCGTGTGGA ACGCANCAGT GGAAAATTCA

2201 TGAGGAGATT TAGACTTCCA GAGAACGCAA AGATGGATCA AGTCAAGGCT

2251 TNTATGGAGA ATGGAGTGCT GACTGTCACT GTTCCAAAGG AAGAAGTGAA