Table S1. RFLP Patterns for Black Grouse Family Members Hybridised with Mhc Class II BLB

Table S1. RFLP patterns for Black grouse family members hybridised with Mhc class II BLB and YLB probes. RFLP bands that were predominantly visible with the YLB probe are highlighted in yellow and RFLP bands that were predominantly visible with the BLB probe are highlighted blue.

Family / Individ. / Probe / 1 / 2 / 3a / 3b / 3c / 4 / 5a / 5b / 6a / 6b / 6c / 7a / 8 / 8b / 9 / RFLP pattern
E Father / D320 / B / │ / ▌ / │ / B
D320 / Y / │ / ▌ / │ / ▌ / │ / │ / P
E Mother / H070 / B / ▌ / ▌ / ▌ / ▌ / ▌ / C
H070 / Y / ▌ / ▌ / ▌ / ▌ / ▌ / │ / ▌ / ▌ / Q
E Offspr. / H070.2 / B / │ / ▌ / │ / │ / A
H070.2 / Y / │ / │ / │ / ▌ / │ / │ / │ / R
E Offspr. / H070.3 / B / │ / ▌ / │ / │ / A
H070.3 / Y / ▌ / ▌ / │ / ▌ / │ / │ / P
E Offspr. / H070.4 / B / ▌ / ▌ / ▌ / ▌ / ▌ / D
H070.4 / Y / ▌ / ▌ / │ / ▌ / ▌ / │ / │ / ▌ / P
E Offspr. / H070.5 / B / │ / │ / │ / │ / │ / D
H070.5 / Y / │ / │ / │ / │ / │ / │ / S
E Offspr. / H070.6 / B / │ / │ / │ / │ / │ / D
H070.6 / Y / │ / │ / │ / │ / │ / S
F Father / D375 / B / │ / ▌ / │ / │ / ▌ / E
D375 / Y / ▌ / ▌ / ▌ / ▌ / █ / ▌ / T
F Mother / H071 / B / │ / ▌ / ▌ / │ / I
H071 / Y / │ / ▌ / ▌ / │ / │ / │ / U
F Offspr. / H071.1 / B / │ / ▌ / │ / │ / ▌ / E
H071.1 / Y / │ / │ / ▌ / │ / ▌ / ▌ / T
F Offspr. / H071.5 / B / │ / ▌ / │ / │ / ▌ / E
H071.5 / Y / │ / │ / ▌ / │ / ▌ / ▌ / T
F Offspr. / H071.4 / B / │ / ▌ / │ / ▌ / F
H071.4 / Y / │ / ▌ / ▌ / ▌ / ▌ / V
F Offspr. / H071.6 / B / ▌ / │ / │ / ▌ / F
H071.6 / Y / │ / │ / │ / ▌ / S
F Offspr. / H071.2 / B / │ / ▌ / │ / │ / ▌ / F
H071.2 / Y / │ / ▌ / ▌ / ▌ / ▌ / V
G Father / D476 / B / │ / │ / │ / │ / G
D476 / Y / │ / │ / │ / │ / ▌ / │ / │ / T
G Offspr. / H085.5 / B / │ / ▌ / │ / ▌ / H
H085.5 / Y / │ / │ / ▌ / │ / ▌ / T
G Offspr. / H085.7 / B / │ / │ / H
H085.7 / Y / │ / │ / │ / │ / │ / T
G Offspr. / H085.8 / B / │ / │ / │ / G
H085.8 / Y / │ / │ / │ / │ / ▌ / │ / T
G Offspr. / H085.2 / B / ▌ / │ / │ / ▌ / │ / G
H085.2 / Y / │ / │ / ▌ / │ / ▌ / ▌ / │ / T
G Offspr. / H085.4 / B / ▌ / ▌ / H
H085.4 / Y / │ / │ / │ / ▌ / S
G Offspr. / H085.6 / B / │ / │ / H
H085.6 / Y / │ / │ / │ / │ / S

20 30 40 50 60 70 80 90

|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

Tete BLb 1 cDNAVRLLERQIYNRQQFAHFDSEVGKYVADTALGELQAEYWNNNTERLEYARGAVDTYCRHNYGVFEPFTVQRS

Tete BLb 2 cDNA ...... F......

Tete BLb 3 cDNA..F...... P...... E..RF...... G......

Tete BLb 4 cDNA..HV..D...... M...... P...... Y...V..S...F...... IL......

Tete BLb 5~~~~~~~~~~~~~~~~~~~~~~~~...... M.....Y..IK.NE...F...... ~~~~~~

Tete BLb 6~~~~~~~~~~~~~~~~~~~~~~~~....P...... D.QFI..KQ.Q..R...... V..~~~~~~

Tete BLb 7~~~~~~~~~~~~~~~~~~~~~~~~....P...... M..D..Y...R..E..R...... IL..~~~~~~

Tete BLb 8~~~~~~~~~~~~~~~~~~~~~~~~....P....P...... D..Y...R..E..R...... V..~~~~~~

Tete BLb 9~~~~~~~~~~~~~~~~~~~~~~~~....P....P...... D..Y...R..E..R...... G..~~~~~~

Gallus BLbI U91530 A.F...H...... M....D...... P...R...I..S.A.I..DEMN....F...... G.S......

Tete YLb 1M.F.V.HV.....YV....D..LF....V...PS.RLF.SQPDV..KN.A..EML.NY.HEIVA.L.L.KR

Tete YLb 2M.FVV.HV.....YV....D..LF....V...PS.RLF.SQPDV..KN.A..EML.NY.HEIVA.L.L.KR

Tete YLb 3M.F.V.HV.....YV....D..LF....V...PS.RLF.SQPDV..KN.A..EML.NY.HEIVA.L.L.KR

Tete YLb 4M.F.V.HV.....YV....D..LF....V...PS.RLF.SQPDV..KN.AV.EML.NY.HEIVA.L.L.KR

Tete YLb 5M.FVV.HV.....YV....D..LF....V...PS.RLF.SQPDV..KN.A..EML.NY.HEIVA.L.L.KR

Gallus YLb AB020332 ..F.V.HV.....YV....D..LF....V...PS.KLF.SQPDV..KN.A..EML.NY..EIVA.L.L..R