Table S1Phosphopeptides identified using nanoLC-MS/MS

Proteins / Gene locus / Peptidesa / E-valueb / Chargesc / MH+ Obsd
phot1 / AT3G45780 / GT(pS)58PQPRPQQEPAPSNPVR / 4.6E-07 / 3 / 2155.0603
N(pS)141GGTENDPNGKK / 7.0E-05 / 3 / 1493.7385
(pS)165SGEMSDGDVPGGR / 9.8E-11 / 2 / 1462.5826
S(pS)166GEMSDGDVPGGR / 3.8E-10 / 2 / 1462.5823
SSGEM(pS)170DGDVPGGR / 1.5E-08 / 2 / 1462.5830
SGIPRV(pS)185EDLK / 9.8E-05 / 2 / 1344.7373
AL(pS)350ESTNLHPFMTK / 1.1E-04 / 3 / 1719.8652
ALSES(pT)353NLHPFMTK / 7.8E-04 / 3 / 1735.8586
SE(pS)364DELPK / 4.7E-04 / 2 / 1048.5067
M(pS)376ENVVPSGR / 7.5E-04 / 2 / 1187.5397
RM(pS)376ENVVPSGR / 3.3E-06 / 2 / 1343.6462
(pS)409SLSFMGIK / 6.6E-04 / 2 / 1113.5875
S(pS)410LSFMGIK / 6.9E-05 / 2 / 1113.5858
KS(pS)410LSFMGIK / 1.1E-06 / 2 / 1273.7382
phot2 / AT5G58140 / APP(pS)9PLNDAESLSER / 2.7E-06 / 2 / 1694.7966
(pS)22LEIFNPSSGK / 2.5E-05 / 2 / 1322.6841
R(pS)22LEIFNPSSGK / 5.8E-09 / 2 / 1478.7867
R(s)105(s)106EESTSSESGAFPR / 3.9E-07 / 2 / 1825.7888
(pS)105SEESTSSESGAFPR / 6.0E-08 / 2 / 1669.6898
(s)105(s)106EESTSSESGAFPR / 9.0E-09 / 2 / 1669.6886
RS(pS)106EESTSSESGAFPR / 3.6E-09 / 2 / 1825.7909
S(pS)106EESTSSESGAFPR / 1.3E-09 / 2 / 1669.6935
SSEEST(s)111(s)112ESGAFPR / 5.2E-03 / 2 / 1669.6965
SSEESTS(pS)112ESGAFPR / 6.3E-12 / 2 / 1669.6820
(pS)284QVQESVSNDTMVKPDSSTTPTPGR / 1.7E-12 / 3 / 2792.3296
QSDEASK(pS)319FR / 1.1E-04 / 2 / 1298.6256
VS(pT)328PTGSK / 8.4E-04 / 2 / 920.4942
D(pS)364WDLSDR / 1.8E-03 / 2 / 1105.4495
MEPEELMLSTEVIGQRD(pS)364WDLSDR / 2.5E-03 / 3 / 2948.3359
JAC1 / AT1G75100 / (pS)23PGGDDVDIDFGDVFGGPPK / 1.5E-13 / 3 / 2134.9856
(pS)45KVTSNEVTR / 4.4E-03 / 2 / 1264.6746
RR(s)45KV(t)48SNEVTR / 9.3E-03 / 2 / 1610.8408
H(pS)56FSESALR / 6.3E-04 / 2 / 1145.5294
VTSNEVTRH(pS)56FSESALR / 5.0E-05 / 3 / 2027.9557
VNE(s)109(s)110(s)111LPGSR / 7.7E-04 / 2 / 1244.5835
VNES(s)110(s)111LPGSR / 4.0E-04 / 2 / 1244.5861
VNESS(pS)111LPGSR / 9.7E-05 / 2 / 1240.5593
IL(pS)119PAHKPESSSGTSSPSQFSLPAK / 2.7E-08 / 3 / 2704.3657
IL(pS)119PAHKPESSSGTS(pS)132PSQFSLPAK / 8.7E-06 / 3 / 2784.3320
ILSPAHKPESSSGT(pS)131(pS)132PSQFSLPAK / 4.9E-03 / 3 / 2784.3291
ETVSS(pS)166PLSR / 8.4E-03 / 2 / 1174.5641
ETVSSSPL(pS)169R / 8.4E-03 / 2 / 1174.5643
NKETVSSSPL(pS)169R / 3.2E-04 / 2 / 1448.7584
T(s)172(s)173KADVVSTAK / 1.7E-05 / 3 / 1369.7737
TS(pS)173KADVVSTAK / 8.2E-09 / 2 / 1369.7726
S(pS)384LDSSPIPDK / 2.7E-10 / 2 / 1289.6494
SSLDS(pS)388PIPDK / 5.4E-05 / 2 / 1289.6466
GASVGAGGESLGQ(pS)437SR / 1.2E-07 / 3 / 1531.7040
GASVGAGGESLGQS(pS)438R / 2.9E-09 / 2 / 1531.7063
PMI1 / AT1G42550 / NFAN(pS)314FGR / 2.4E-03 / 2 / 1024.4545
T(pS)323FSVPSPK / 9.1E-04 / 2 / 1093.5773
T(pS)323FSVP(pS)328PK / 8.3E-03 / 2 / 1173.5420
TSF(pS)325VPSPK / 5.0E-03 / 2 / 1085.527832
TSFSVP(pS)328PK / 3.4E-03 / 2 / 1093.5786
GVEFDDDLETEK(pS)407DGTIGER / 2.7E-11 / 3 / 2356.0679
DESDGGDGETE(pS)464QRLDEEEQTVTK / 1.2E-07 / 3 / 2798.1948
SGE(pS)507VDDESENYLSDLGK / 1.2E-18 / 2 / 2087.9155
PMI2/WEB2 / AT1G66840 / TMLEEERE(pS)513FR / 1.4E-05 / 3 / 1534.6617
FKGNSEDNGLIN(pS)545PKPVR / 4.8E-06 / 3 / 2148.1550
GNSEDNGLIN(pS)545PKPVR / 5.3E-14 / 3 / 1840.9404
SVRL(pS)556GK / 3.9E-03 / 2 / 882.4816
WEB1 / AT2G26570 / FSG(pS)148PVSTGTPK / 5.4E-06 / 2 / 1308.6689
FSG(pS)148PVSTG(pT)154PK / 1.0E-04 / 2 / 1388.6365
FSGSPVSTG(pT)154PK / 2.9E-06 / 2 / 1308.6638
NLKE(pS)744FEGGK / 5.3E-07 / 2 / 1284.6991
KAC1 / AT5G10470 / K(s)23(s)24SNASFAESTGHR / 1.4E-08 / 3 / 1709.8103
KS(s)24(s)25NASFAESTGHR / 4.6E-08 / 3 / 1701.7594
RN(pS)46ISTPSLPPK / 2.9E-03 / 2 / 1440.8073
RNSISTP(pS)51LPPK / 1.3E-03 / 2 / 1440.8070
SMAVSTQVL(pS)668PSLR / 3.7E-07 / 2 / 1587.8129
A(pS)674PNIQPANVNR / 4.4E-06 / 2 / 1392.6930
GS(pS)842PGRSPVR / 1.6E-03 / 2 / 1107.5308
GS(pS)842PGR(pS)846PVR / 2.0E-05 / 2 / 1191.5221
GSSPGR(pS)846PVR / 2.8E-04 / 2 / 1111.5552
SDAALLNLEEGS(pS)1051PIPNPSTAAEDSR / 2.6E-16 / 3 / 2753.2830
KAC2 / AT5G65460 / K(pS)22PSSEEGVHR / 1.4E-04 / 3 / 1356.6757
KSP(pS)24SEEGVHR / 3.0E-04 / 3 / 1348.6261
SVASSTQVS(pS)658PSSK / 8.2E-06 / 2 / 1495.7480
SSSG(pS)822SSPGR(pS)828PVR / 3.3E-03 / 2 / 1539.6554
SSSGS(pS)823(pS)824PGRSPVR / 5.1E-03 / 2 / 1539.6506
SSSGS(pS)823SPGR(pS)828PVR / 7.6E-07 / 2 / 1539.6499
SSSGSS(pS)824PGRSPVR / 1.5E-03 / 2 / 1455.6599
SSSGSS(pS)824PGR(pS)828PVR / 5.7E-03 / 2 / 1539.6550
SDAALF(pT)1027LEEGSSPVQNPSTAAEDSR / 9.5E-13 / 3 / 2791.2512
CHUP1 / AT3G25690 / NYQ(pT)367PAGK / 1.6E-05 / 2 / 1014.4645
LMLEYAG(pS)399ER / 2.4E-05 / 2 / 1296.5851
SKDD(pS)458SVQSSPSR / 2.0E-03 / 3 / 1523.7188
SKDD(s)458(s)459VQS(s)463PSR / 1.2E-04 / 2 / 1603.6852
SKDDS(pS)459VQS(pS)463PSR / 5.2E-05 / 2 / 1595.6370
DDSSVQS(pS)463PSR / 5.3E-06 / 2 / 1276.5355
SKDDSSVQS(pS)463PSR / 1.1E-04 / 3 / 1523.7177
SFYGG(pS)472PGR / 1.4E-03 / 2 / 1039.4561
LSS(pS)479MNK / 1.2E-03 / 2 / 910.4554
THRUMIN1 / AT1G64500 / (s)113A(s)115FHTLDELEVR / 1.2E-04 / 3 / 1615.7687
K(s)113A(s)115FHTLDELEVR / 9.8E-06 / 3 / 1775.9198
SA(pS)115FHTLDELEVR / 1.9E-09 / 2 / 1615.7667
KSA(pS)115FHTLDELEVR / 1.1E-04 / 3 / 1767.8658
TESTQS(pS)164YSGPR / 2.2E-05 / 2 / 1411.6003
TESTQS(s)164Y(s)166GPR / 5.2E-05 / 2 / 1407.5770
TESTQSSY(pS)166GPR / 3.7E-06 / 2 / 1411.6108
NPH3 / AT5G64330 / APSPSTTNFAGS(pS)223PR / 4.3E-04 / 2 / 1588.7311
DSSFYC(pS)237PSR / 9.8E-06 / 2 / 1317.5125
LLEHFLVQEQTEGS(pS)467PSR / 3.9E-09 / 3 / 2169.0500
M(pS)472PSPSQSMYADIPR / 4.8E-04 / 2 / 1778.7762
MSP(pS)474PSQSMYADIPR / 2.0E-12 / 2 / 1774.7570

a- A peptide is considered to be new when absent from PhosphAt 4.0 database ( They are shown in bold.

b- Calculated empirically with X!Tandem

c- Precursor charge

d- Monoisotopic protonated peptide


Fig. S1Identification of new phosphorylation sites in chloroplast movement proteins by mass spectrometric sequencing

Fig. S2Quantitative reverse transcription-polymerase chain reaction on THRUMIN1 mRNA in transformed thrumin1 mutant leaves.thrumin1plants (SALK_27277) were transformed with either 35S::THRUMIN1 (C). 35S::THRUMIN1_P- (XA) or 35S::THRUMIN1_P+ (XD). THRUMIN1_P+ represents a version of THRUMIN1 where identified phosphorylated residues were replaced by phosphorylation-mimicking aspartate and THRUMIN1_P- by nonphosphorylable alanine. Data are representative of three independent biological experiments.