PERSONAL INFORMATION
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Name: Ali Moradi
Birth place: Yazd
Birth Date: 1973
Sex: Male
Marital statuse: Maried
Address: Department of Biochemistry, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran, 14115-175
Tel: o912 6706056
E-mail:
Education:
2008 (Educating), Ph.D. Biochemistry, Department of Biochemistry, faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
2002, M.Sc. Biochemistry, Department of Biochemistry, faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
1995, B.Sc. Botany, Department of biology, Esfehan University, Esfehan, Iran
Thdsis research:
M.Sc Thesis: Purification of hCG from pregnant urine and separation of it’s subunits. Research Supervisor: Dr. manoochehr mirshahi
Ph.D. Thesis: Creation of a firefly luciferase with novel properties (red shift) by saturation site directed mutagenesis, Research Supervisor: Dr. Saman Hosseinkhani
Research Experience:
1. Essential Molecular Genetics Techniques Such as gene cloning, Electroporation and Cacl2 Transformation, DNA extraction, Gel Electrophoresis
2. Protein over-expression, Protein purification (FPLC and HPLC), SDS-PAGE, Western blot, ELISA
3. Structural studies by UV-Vis, Fluorescence spectrophotometer and Circular Dichroism Spectopolarimetery
4. Site directed mutagenesis, random mutagenesis, DNA-Shuffling
5. Determination of enzymes kinetic properties
Certificates:
· ATP-Assay and microbial screening based on bioluminescence method, 2007, Tarbiat Modares University
· Micro satellite analysis & SSCP based mutation screening, 2005, Elchrom Scientific Switzerland in institute pasture of Iran
Job Experience:
· Work in Iranian Blood Research and Fractionation Company (Isolation of albumin and blood factors from plasma), 1996-2000
· Work in institute pasture of Iran ( Construction, expression and purification of recombinant insulin), 2002-2005
Full papers:
1. Moradi, A., Hosseinkhani, S., Naderi-Manesh, H., Sadeghizadeh, M., Alipour, B.S. The effect of charge distribution in a flexible loop on bioluminescence color of firefly luciferases. Biochemistry 2009; 48: 575–582
2. Alipour, B.S., Hosseinkhani, S., Ardestani, S.K., Moradi, A. The effective role of positive charge introduction in bioluminescence color and thermostability of firefly luciferase. Photochem. Photobiol. 2009; 8(6):847-55
3. Maghami, P., Ranjbar, B., Hosseinkhani, S., Ghasemi, A., Moradi, A., Gill, P. Relationship between stability and bioluminescence color of firefly luciferase. Photochem. Photobiol. Sci. 2010; 9: 376-383
Selected Abstracts:
1. Moradi A., hosseinkhani S., Investigation of a flexible loop role in determination of light color in firefly luciferase by site directed mutagenesis, The 9th Iranian congress of biochemistry & the 2nd international congress of biochemistry and molecular biology, 2007, Shiraz, Iran
2. Moradi A., Mirshahi M., Naderi-Manesh H., Purification of hCG from pregnant urine and separation of it’s subunits, 2002, Ahvaz, iran
3. Moradi A., Aliahmadi A., Omidi M., Sardari S., Jebelli M.R., Sadeghcheh T., Adeli A., Mahboudi F., Disulfide bonds assay of insulin recombinant by ellman method, 4th national Biotechnology congress, 2005, Kerman, Iran
4. Aliahmadi A., Moradi A., Omidi M., Sardari S., Taherieh A., Jebelli M.R., Sadeghcheh T., Adeli A., Mahboudi F., A Modified Acid Precipitation Procedure for Purification and Refolding of Recombinant Human Proinsulin, 4th national Biotechnology congress, 2005, Kerman, Iran
5. Abachi M., Moradi A., Omidi M., Aliahmadi A., Taherieh A., Sadeghche T., Jebelli M.R., Azami M., Sardari S., Mahboudi F., Cloning and expression of human recombinant insulin in E. coli, 4th national Biotechnology congress, 2005, Kerman, Iran
6. Sadeghcheh T., Aliahmadi A., Moradi A., Omidi M., Azami M., Abachi M., Sardari S., Jebelli M.R., Adeli A., Mahboudi F., Kinetic Study of Trypsin Enzymatic Effect on Digestion of Refolded Recombinant Insulin by HPLC, Elisa, Non-Denaturing and Tris-Tricin PAGE Methods, 4th national Biotechnology congress, 2005, Kerman, Iran
7. Omidi M., Sardari S., Zakeri M., Vaziri B., Aliahmadi A., Moradi A., Jebelli M.R., Mahaboudi F., Method Optimization of Reverse Phase High Performance Liquid Chromatography (RP-HPLC) for Characterization of Digested Products of Recombinant Pro-Insulin, 4th national Biotechnology congress, 2005, Kerman, Iran
8. Sardari s., Peirovi A., Moradi A., Vaziri B., Aliahmadi A., Jebeli M.R., Omidi M., Abachi M., Kiani N., Mahboudi F., Sulfitolysis efficiency in downstream processing of recombinant human insulin production and estimating the secondary structure by circular dichroism (CD), 4th national Biotechnology congress, 2005, Kerman, Iran
9. Abachi M., Omidi M., Aliahmadi A., Moradi A., Taherieh A., Sadeghche T., Jebelli M.R., Azami M., Sardari S., Mahboudi F., The construction of a novel proinsulin with mini C – peptide for development of recombinant insulin production, 4th national Biotechnology congress, 2005, Kerman, Iran
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