Electronic Supplementary Material
Differential stability of photosynthetic membranes and fatty acid composition at elevated temperature in Symbiodinium
Díaz-Almeyda E, Thomé PE, El Hafidi M, Iglesias-Prieto R
Table 1. Percentage of fatty acids extracted from whole cells of Symbiodinium microadriaticum A1 and Symbiodinium sp. C1, grown at 24 ºC and 31ºC.
Symbiodinium sp. (C1) / S. microadriaticum (A1)Fatty Acid / 24 ºC / 31 ºC / 24 ºC / 31 ºC
C16:0 / 30.0 / ± / 3.9 / 32.8 / ± / 1.7 / 34.8 / ± / 1.2 / 27.3 / ± / 2.4
C16:1 (n-9) / 2.1 / ± / 0.7 / 2.6 / ± / 0.6 / 1.8 / ± / 0.1 / 3.0 / ± / 1.2
C18:0 / 19.9 / ± / 1.7 / 17.9 / ± / 2.4 / 20.9 / ± / 1.5 / 22.2 / ± / 1.2
C18:1 (n-9t) / 4.7 / ± / 1.9 / 3.7 / ± / 2.0 / 3.1 / ± / 2.6 / 7.3 / ± / 0.6
C18:1 (n-9c) / 11.1 / ± / 3.8 / 10.8 / ± / 3.8 / 10.2 / ± / 2.8 / 8.9 / ± / 2.0
C18:1 (n-7c) / 4.7 / ± / 1.4 / 4.2 / ± / 0.9 / 2.0 / ± / 0.7 / 2.6 / ± / 0.9
C18:2 (n-6) / 3.1 / ± / 2.7 / 2.5 / ± / 0.5 / 5.3 / ± / 2.4 / 2.5 / ± / 1.2
C18:3 (n-3) / 14.0 / ± / 1.8 / 12.1 / ± / 4.9 / 4.6 / ± / 3.2 / 22.8 / ± / 4.4
C20:1 / 0.2 / ± / 0.1 / 0.2 / ± / 0.1 / 0.2 / ± / 0.3 / 0.1 / ± / 0.0
C18:4 (n-3) / 4.9 / ± / 2.5 / 6.0 / ± / 1.8 / 9.7 / ± / 1.8 / 4.4 / ± / 0.6
C22:5 (n-3) / 0.8 / ± / 0.4 / 0.7 / ± / 0.3 / 0.3 / ± / 0.2 / 0.4 / ± / 0.0
C22:6 (n-3) / 4.4 / ± / 1.2 / 7.0 / ± / 2.1 / 7.1 / ± / 0.7 / 2.7 / ± / 1.6
SFAsa / 49.9 / ± / 5.3 / 50.7 / ± / 4.0 / 55.7 / ± / 0.5 / 49.4 / ± / 3.5
MUFAsb / 22.8 / ± / 4.9 / 21.5 / ± / 5.0 / 17.3 / ± / 5.4 / 21.9 / ± / 1.8
PUFAsc / 27.3 / ± / 0.4 / 28.4 / ± / 5.7 / 27.0 / ± / 5.6 / 28.6 / ± / 5.2
C16:0 to C16:1d / 6.4 / ± / 1.4 / 7.3 / ± / 1.6 / 5.0 / ± / 0.2 / 9.9 / ± / 3.8
C18:0 to C18:1d / 35.5 / ± / 9.2 / 37.2 / ± / 9.5 / 32.5 / ± / 7.5 / 28.4 / ± / 3.8
C18:2 to C18:3e / 74.8 / ± / 6.9 / 73.3 / ± / 6.7 / 66.1 / ± / 15.3 / 86.3 / ± / 8.4
C18:3 to C18:4f / 25.2 / ± / 11.9 / 34.2 / ± / 11.8 / 69.2 / ± / 14.8 / 20.6 / ± / 7.9
ΣC16 / 32.1 / ± / 4.7 / 35.4 / ± / 1.7 / 36.6 / ± / 1.3 / 30.3 / ± / 2.5
ΣC18 / 62.4 / ± / 5.3 / 57.2 / ± / 3.4 / 55.7 / ± / 2.1 / 66.6 / ± / 4.2
ΣC22 / 5.3 / ± / 0.8 / 7.7 / ± / 2.0 / 7.4 / ± / 0.9 / 3.1 / ± / 1.6
aSFAs, saturated fatty acids. bMUFAs, monounsaturated fatty acids. cPUFAs, polyunsaturated fatty acids.
dΔ9-desaturase index. eΔ15-desaturase index. fΔ6-desaturase index.
Table 2. Percentage of fatty acids extracted from a fraction enriched in photosynthetic membranes prepared from Symbiodinium microadriaticum A1 and Symbiodinium sp. C1, grown at 24 ºC and 31 ºC.
Symbiodinium sp. (C1) / S. microadriaticum (A1)Fatty Acid / 24 ºC / 31 ºC / 24 ºC / 31 ºC
C16:0 / 32.6 / ± / 2.8 / 34.6 / ± / 2.8 / 40.7 / ± / 4.7 / 35.1 / ± / 2.4
C16:1 (n-9) / 2.2 / ± / 0.4 / 2.48 / ± / 0.5 / 2.5 / ± / 0.5 / 1.8 / ± / 0.2
C18:0 / 21.4 / ± / 2.7 / 20.6 / ± / 2.0 / 20.2 / ± / 2.3 / 21.0 / ± / 1.8
C18:1 (n-9t) / 6.0 / ± / 2.9 / 3.51 / ± / 1.6 / 1.8 / ± / 1.1 / 5.3 / ± / 2.3
C18:1 (n-9c) / 10.7 / ± / 0.5 / 7.32 / ± / 1.2 / 5.1 / ± / 1.0 / 9.7 / ± / 1.1
C18:1 (n-7) / 5.2 / ± / 0.4 / 5.14 / ± / 1.0 / 2.8 / ± / 0.9 / 2.6 / ± / 1.3
C18:2 (n-6) / 2.8 / ± / 1.6 / 2.39 / ± / 0.8 / 1.5 / ± / 0.5 / 3.2 / ± / 1.5
C18:3 (n-3) / 8.5 / ± / 3.2 / 9.12 / ± / 3.6 / 4.8 / ± / 1.4 / 8.1 / ± / 4.5
C20:1 / 0.1 / ± / 0.0 / 0.2 / ± / 0.2 / 0.1 / ± / 0.1 / 0.1 / ± / 0.0
C18:4 (n-3) / 5.4 / ± / 1.7 / 7.79 / ± / 3.5 / 10.4 / ± / 3.9 / 8.3 / ± / 3.5
C22:5 (n-3) / 0.4 / ± / 0.1 / 0.43 / ± / 0.1 / 0.3 / ± / 0.1 / 0.4 / ± / 0.3
C22:6 (n-3) / 4.8 / ± / 2.5 / 6.43 / ± / 2.9 / 9.9 / ± / 1.9 / 4.5 / ± / 1.7
SFAsa / 53.9 / ± / 5.5 / 55.2 / ± / 2.9 / 60.8 / ± / 6.3 / 56.1 / ± / 3.2
MUFAsb / 24.2 / ± / 3.3 / 18.7 / ± / 2.1 / 12.3 / ± / 2.1 / 19.4 / ± / 4.0
PUFAsc / 21.9 / ± / 6.8 / 26.2 / ± / 2.7 / 26.8 / ± / 4.3 / 24.5 / ± / 1.5
C16:0 to C16:1d / 6.3 / ± / 0.7 / 6.77 / ± / 1.2 / 5.9 / ± / 1.3 / 4.8 / ± / 0.6
C18:0 to C18:1d / 33.8 / ± / 3.6 / 26.1 / ± / 1.3 / 20.3 / ± / 4.9 / 31.7 / ± / 4.5
C18:2 to C18:3e / 73.6 / ± / 9.7 / 77.3 / ± / 2.6 / 75.8 / ± / 11.4 / 68.7 / ± / 14.4
C18:3 to C18:4f / 41.6 / ± / 17.0 / 48 / ± / 20.9 / 68.1 / ± / 5.8 / 51.6 / ± / 23.4
ΣC16 / 34.8 / ± / 3.2 / 37.1 / ± / 3.0 / 43.2 / ± / 4.7 / 36.9 / ± / 2.4
ΣC18 / 59.9 / ± / 2.3 / 55.9 / ± / 5.3 / 46.5 / ± / 5.8 / 58.1 / ± / 3.8
ΣC22 / 5.2 / ± / 2.6 / 6.86 / ± / 2.9 / 10.2 / ± / 1.8 / 4.9 / ± / 1.4
aSFAs, saturated fatty acids. bMUFAs, monounsaturated fatty acids. cPUFAs, polyunsaturated fatty acids.
dΔ9-desaturase index. eΔ15-desaturase index. fΔ6-desaturase index.