Suppl. Table 1. Chromosomal location of the Adzuki bean SSRs used in VBN(Gg)2 * KMG189 Population
Chromosome 1S. No / SSR locus name / Forward Primer ( 5’-3’) / Reverse Primer( 5’-3’)
1 / CEDG087 / CCTCTTGAAATTCTCCTTGA / CCTCTTGTGAACCTCAATAA
2 / CEDG149 / GGCTGAAGGTGATGACAGAAG / GGCACTGGTTTTCTAAGGTTGTTG
3 / CEDG001 / ACTATGCAGAAAGACGCTCC / GGCTCTCTCTTTCTCCATTC
4 / CEDG048 / TCTCTTCCTCTATGGCTTGG / GCTCCTCTTTTTGCTGCATC
5 / CEDG090 / ATAAGTAGAAATTGGTTCAAATG / GGTTCGTTAAAGTAACTTTTAAT
6 / CEDG148 / GGGAAGAAGGAAGAAGGAACC / GTGACAAAACACCTGTAGCCATCC
7 / CEDG254 / CGATGTCTCTTGCTTCAAGG / GTGAAGGACTAGCCAAGTTTG
8 / CEDC024 / CATCTTCCTCACCTGCATTC / TTTGGTGAAGATGACAGCCC
9 / CEDG133 / GCATACATAATGTGGTGAGATG / GTCTCGTGCCTTTCACAC
10 / CEDG039 / CTATCGAAGTAATACATTCAAAGC / GATCTATGCATGGATCTAGCATC
11 / CEDC027 / ACTGGATGAGGGTTTAGTGCG / CTGTCTTGTCTTGTGGGTTCGTTC
Chromosome 2
12 / CEDG050 / TCCCACTTCTCCATTACCTCCAC / GAGATTATCTTCTGGGCAGCAAGG
13 / CEDG108 / TCCCAGCTACCCACCTCT / CTTCTACCCAGCCAAACC
14 / CEDC012 / TTTAAGCAGAGACAGTTGAC / CGCCATTGTTGATATTAAGC
15 / CEDG29 / GATTGCTTTTAGCAGAGGGC / GAAGAAACCCATCTCGATCC
16 / CEDG225 / GAGGAAGTGTTGCAGCACC / GTAGACTCTGCAGAGGGATG
17 / CEDG026 / TCAGCAATCACTCATGTGGG / TGGGACAAACCTCATGGTTG
18 / CEDG065 / GGAATTTTGAGAACGGATTTGC / CCACCGACCACGGCCTTC
19 / CEDG108 / TCCCAGCTACCCACCTCT / CTTCTACCCAGCCAAACC
20 / CEDG210 / GAACCCACTTCTGAAGTTC / GAACAACCTCTGCAGTAG
21 / CEDG261 / GGTCCCAAAATCACCCAG / GGTTCATTTGGAGCACTGAG
Chromosome 3
22 / CEDG043 / AGGATTGTGGTTGGTGCATG / ACTATTTCCAACCTGCTGGG
23 / CEDG117 / GTACACTTCCACTAATCCAAAATT / TGGTACCTTCCTTATCTGAAATTA
24 / CEDG186 / GGATGGGAGAGTAAGAAG / GCATGGCATGATGACTTG
Chromosome 4
25 / CEDG088 / TCTTGTCATTTAGCACTTAGCACG / TTGTTGTTTACTAAGAGCCCGTGT
26 / CEDG139 / CAAACTTCCGATCGAAAGCGCTTG / GTTTCTCCTCAATCTCAAGCTCCG
27 / CEDG008 / AGGCGAGGTTTCGTTTCAAG / GCCCATATTTTTACGCCCAC
28 / CEDG165 / GCTCTGTCAGTTCCCACTAC / GGTCCTGAACCCAGATGAAC
29 / CEDG181 / CGCGAGATCTGGATCGTTGATC / GCAGTACGGTAACGTCCTTGAC
30 / CEDC055 / CAAACACTTTTGTAACTCCC / GCTTCTAACCTTGATCCTTC
31 / CEDG127 / GGTTAGCATCTGAGCTTCTTCGTC / CTCCTCACTTGGTCTGAAACTC
32 / CEDG232 / GATGACCAAGGTAACGTG / GGACAGATCCAAAACGTG
Chromosome 5
33 / CEDG014 / GCTTGCATCACCCATGATTC / AAGTGATACGGTCTGGTTCC
34 / CEDG018 / AGCGTGTTTGTGGTGATAGC / ACACAGGAACGAACAAACCC
35 / CEDG115 / GGCTCATTGTACCACTGGATAT / ATGCCTCCTTTCAGGTGATTGT
36 / CEDG264 / GATTCCCTTCCTAGCTATGG / CTGCTGGACATGAAGATTCAG
Chromosome 6
37 / CEDG191 / CAATAAGCAATCTGTGGAGAG / CTGCAGGAAACTTGGAATTGC
38 / CEDC024 / CATCTTCCTCACCTGCATTC / TTTGGTGAAGATGACAGCCC
39 / CEDG015 / CCCGATGAACGCTAATGCTG / CGCCAAAGGAAACGCAGAAC
40 / CEDG248 / CAGAACACAAAAGGGTTCTCG / GTGGATTCACTCGCTTCC
Chromosome 7
41 / CEDG174 / GAGGGATCTCCAAAGTTCAACGG / GAAGGCTCCGAAGTTGAAGGTTG
42 / CEDG131 / CCTTTTTCTTCCTACCCTCTACC / CACCACCTAGCTGTTGCTAG
43 / CEDG016 / ACTCTTGTCAATTGTCCAGG / TAACTTGTCACTGGAAAGGC
44 / CEDG064 / TGTAAGGTCACTTTGGCCTCAAG / TTAAGTTGACTCGTTGCCCTTTG
45 / CEDG085 / AGCATGGAATCTCAGACTGAGACA / AACAAGATCGAAGAAGTCGCTCAC
46 / CEDG111 / TGGAAGTTTCCAAGAGGGTTTTC / TCTCACCACCTTTTACCTTCTCA
47 / CEDG143 / GATGAACTCGTCTCGCTCATCG / CTGGACGCGTCTACTCAGAC
48 / CEDG201 / CGGGTAGACAAAGAGATACACG / CTAGCAGAAACAGGAGATCCTC
49 / CEDG203 / GACTGAACCTATGCGGTCCAAC / CAACGTGTTAGCCTTCTTGCCTC
50 / CEDG215 / CGTACTGAGATTGAGGTC / CACCATGTGTTCCTCAAG
51 / CEDG041 / GCTGCATCTCTATTCTCTGG / GCCAACTAGCCTAATCAG
52 / CEDG174 / GAGGGATCTCCAAAGTTCAACGG / GAAGGCTCCGAAGTTGAAGGTTG
Chromosome 8
53 / CEDG247 / GTAGACACTGATCATCACC / GACCATCATCGATACGATTC
54 / CEDG033 / GTGAGGTAGCTATGTAGCAC / ACTGGACCGACAAGAGTAAG
55 / CEDG071 / GGTCCATTGAGACGGATCGAG / TCCCACCTCAGCGGAATCC
56 / CEDG125 / TGGAATATACTGTTTAATAGAG / AGATTAATTTGATCACTCATTC
57 / CEDG251 / ATATCTCAAAACCCTTCCTG / CCTCAATAACAATGATACGAC
58 / CEDG257 / GACTACTCTCAAGACCAAAG / GATGGTTGTAGATAACACTCC
59 / CEDG265 / GTAAAACAAACACACAGGAC / GCTCTCAACGAGAATGAAC
60 / CEDG269 / CTGTTACGGCACCTGGAAAG / GCAGAGACACACCTTAACCTTG
61 / CEDG030 / TGAGGGAATGGGAGAGAGGC / TCCGCAGATAGAGGCTCACG
Chromosome 9
62 / CEDG056 / TTCCATCTATAGGGGAAGGGAG / GCTATGATGGAAGAGGGCATGG
63 / CEDC011 / GTCCGACTTTATGTGTGGAG / TTTCTAGTTCCAGCCCCGAC
64 / CEDG022 / AGGAATGTGAGATTTG / AATCGCTTCAAGGTCAAGCC
65 / CEDC080 / CACGTTGGAGGAAGTGACGC / CATCGCCACCACAGAACCA
66 / CEDG166 / GGTACAACATTCTTCTATTTG / GGCTTATGAGTTTATCTTATC
67 / CEDG172 / GCTGACGTAGGTGACAACC / CGGCTTGTGCTTCATTGTCTG
68 / CEDG173 / GATAAGAGATGCATCACTC / CTTCTCTTCCATCACATCTG
69 / CEDG262 / GATGAGCCGAATTCGGTGTC / CTGCTTCACCTCGTGACC
70 / CEDG024 / CATCTTCCTCACCTGCATTC / TTTGGTGAAGATGACAGCCC
71 / CEDG238 / GCAGAATTTGACTGCTAGAAAGC / CCATACATTTGTTGCACGCATG
Chromosome 10
72 / CEDG150 / GAAGGGAATGAAAATGAAACCC / GTTCAATCCATTCAGTCTCC
73 / CEDG134 / CTCCGTGTTGAAACAATGACG / GGTCTTTCTGATCTACGAACTTG
74 / CEDG147 / CTCCGTCGAAGAATTGGTTGAC / GCAAAAATGTGGCGTTTGGTTGC
75 / CEDG081 / TGTGGGTGTTTTATGCTTTGTG / GTATTCGGTCATTCGATCTTAC
76 / CEDG116 / TTGTATCGAAACGACGACGCAGAT / AACATCAACTCCAGTCTCACCAAA
77 / CEDG134 / CTCCGTGTTGAAACAATGACG / GGTCTTTCTGATCTACGAACTTG
78 / CEDG147 / CTCCGTCGAAGAATTGGTTGAC / GCAAAAATGTGGCGTTTGGTTGC
79 / CEDG198 / CAAGGAAGATGGAGAGAATC / CCTTCTAAGAACAGTGACATG
80 / CEDG243 / GACAACTCATCCATTCTTGAG / CCTATGGATAGTGATACAGC
Chromosome 11
81 / CEDG100 / CCCATCAAGTAACTACATAACA / ATGTGGGACTGGACAAATAAAA
82 / CEDG014 / GCTTGCATCACCCATGATTC / AAGTGATACGGTCTGGTTCC
83 / CEDG042 / CACAGTGGTTTGGGCAACAG / TCAGAGGTTCCCATTTCCCG
84 / CEDG168 / CTGCTTGGTGTTGAAGCTTC / CATTCTACATTCCAGACCTGC
85 / CEDG014 / GCTTGCATCACCCATGATTC / AAGTGATACGGTCTGGTTCC
86 / CEDG044 / TCAGCAACCTTGCATTGCAG / TTTCCCGTCACTCTTCTAGG
Suppl. Table 2. Average infectivity percentage for MYMV in the field selected resistant entries and VBNGg 2
Serial Number / Name of the mungbean lines / Culture name / No. of days taken forsymptom development / Average infectivity %
1. (R*) / SP 84 / VA221
VA239 / -
19 / 0.00
55.50
2. (R) / MYMV R 30 / VA221
VA239 / 18
18 / 47.77
49.00
3. (R) / MYMV R 47 / VA221
VA239 / 19
21 / 49.88
66.50
4. (R) / Co 5 (Bg) / VA221
VA239 / 21
23 / 100.00
100.00
5. (R) / KMG 189 / VA221
VA239 / -
21 / 0.00
40.00
6. (R) / ML818 / VA221
VA239 / 19
- / 65.00
00.00
7. (R) / ML 1108 / VA221
VA239 / -
19 / 00.00
55.55
8. (R) / MYMVR27 / VA221
VA239 / -
20 / 0.00
60.00
9. (R) / MYMVR28 / VA221
VA239 / -
- / NG*
0.00
10. (R) / MYMVR 46 / VA221
VA239 / -
- / 0.00
NG*
11. (R) / MYMVR56 / VA221
VA239 / - / NG*
12. (R) / MYMVR 111 / VA221
VA239 / - / NG*
13. (R) / MYMVR 115 / VA221
VA239 / - / 0.00
NG*
14. (S*) / VBN 2(Gg) / VA221
VA239 / 21
21 / 66.50
42.50
* R – Resistant line, S – Susceptible line and NG – No Germination
Suppl. Fig. 1 and Suppl. Fig. 2
Suppl. Fig 3.
Supple. Table 3a. Variation for phenological traits among the F2 Mapping population of VBNGg 2 X KMG 189
S.No / Morphological character / Parent 1VBNGg 2 / Parent 2
KMG 189 / Mean / Range
Min-Max / Standard Variation / Standard Error / Standard deviation / Confidence
1% / 5%
1. / No. of seeds per pod / 11** / 9** / 8.92** / 4 - 13 / 6.1 / 0.26 / 2.47 / 0.69 / 0.52
2. / No. of pods/plant / 29** / 22** / 21.36** / 5 - 43 / 76.3 / 0.92 / 8.73 / 2.42 / 1.83
3. / Pod length / 7** / 6.5** / 6.55** / 5.5 – 7.5 / 0.22 / 0.05 / 0.47 / 0.13 / 0.10
4. / No. of Productive branches / 10.2** / 6.2** / 9.36** / 5 - 13 / 5.92 / 0.26 / 2.43 / 0.67 / 0.51
5. / Leaf length / 9.96** / 7.94** / 9.02** / 7 - 11 / 1.16 / 0.11 / 1.08 / 0.30 / 0.23
6. / Leaf breadth / 6.8** / 5.9** / 6.08** / 4 – 7.5 / 0.78 / 0.09 / 0.89 / 0.25 / 0.19
7. / Plant height / 41.8** / 43.8** / 40.13** / 30.5 -47.5 / 14.78 / 0.41 / 3.84 / 1.07 / 0.80
**- Confidence at 1% level
Supple. Table 3b. Variation for phenological traits among the F3 mapping population of VBNGg 2 X KMG 189
S.No / Morphological character / Parent 1VBNGg 2 / Parent 2
KMG 189 / Mean / Range
Min-Max / Standard Variation / Standard Error / Standard deviation / Confidence
1% / 5%
1. / No. of seeds per pod / 11** / 9** / 10.33** / 9 - 13 / 1.67 / 0.33 / 1.29 / 0.99 / 0.71
2. / No. of pods/plant / 29 / 22 / 24.2 / 12 - 64 / 150.45 / 3.167 / 12.27 / 9.42 / 6.79
3. / Pod length / 7** / 6.5** / 11.24** / 6.1 - 7.7 / 0.134 / 0.094 / 0.366 / 0.28 / 0.20
4. / No. of Productive branches / 10.2** / 6.2** / 9.6** / 5 - 18 / 10.4 / 0.832 / 3.22 / 2.48 / 1.78
5. / Leaf length / 9.96** / 7.94** / 7.76** / 6.8 – 10.2 / 0.845 / 0.237 / 0.92 / 0.71 / 0.51
6. / Leaf breadth / 6.8** / 5.9** / 6.15** / 5.2 – 7.3 / 0.336 / 0.149 / 0.58 / 0.45 / 0.32
7. / Plant height / 41.8 / 43.8 / 37.2 / 25 – 46.3 / 43.36 / 1.7 / 6.58 / 5.06 / 3.64
** - Confidence at 1% level
Supple. Table 4 Individual component (factor) analysis for the morphological traits under study for principle component analysis
F1 / F2 / F3 / F4 / F5 / F6 / F7 / F8plant height / 0.959 / -0.073 / 0.034 / -0.168 / 0.037 / -0.082 / 0.191 / -0.034
root length / 0.960 / -0.131 / -0.193 / 0.017 / -0.003 / 0.037 / 0.024 / 0.150
leaf bredth / 0.962 / -0.028 / 0.055 / -0.131 / 0.036 / -0.153 / -0.169 / 0.014
leaf length / 0.936 / 0.067 / 0.136 / 0.285 / 0.124 / -0.062 / 0.031 / 0.005
No: of seeds per pod / 0.961 / 0.000 / -0.099 / -0.061 / 0.173 / 0.160 / -0.051 / -0.068
No: pods per plant / 0.945 / -0.062 / -0.232 / 0.117 / -0.157 / -0.044 / -0.015 / -0.092
pod length / 0.921 / -0.204 / 0.283 / 0.001 / -0.132 / 0.110 / -0.020 / 0.000
no.of productive branches / 0.879 / 0.462 / 0.029 / -0.055 / -0.090 / 0.040 / 0.011 / 0.025
Eigenvalue / 7.078 / 0.287 / 0.205 / 0.147 / 0.098 / 0.077 / 0.070 / 0.037
Variability (%) / 88.478 / 3.588 / 2.561 / 1.843 / 1.229 / 0.957 / 0.874 / 0.468
Cumulative % / 88.478 / 92.066 / 94.627 / 96.471 / 97.700 / 98.657 / 99.532 / 100.000
Graph for the supple. Table 4
Supple. Table 5a.Percentage of Variability, Heritability and Genetic advance analysis for F2 Population in the intervarietal cross of (VBNGg 2 x KMG 189)
S.No / Plant Character / PCV % / GCV% / h2 % / GA as % of Mean1. / Plant height / 6.03 / 2.95 / 24.00 / 4.71
2. / Leaf length / 3.57 / 1.00 / 7.87 / 2.34
3. / Leaf Breadth / 3.56 / 2.08 / 34.31 / 8.39
4. / No. of seeds per pod / 8.21 / 6.08 / 68.47 / 38.81
5. / No. of Pods per plant / 18.79 / 16.29 / 75.14 / 62.96
6. / Pod length / 1.83 / 0.39 / 4.49 / 0.664
7. / No. of productive branches / 7.90 / 4.20 / 28.22 / 15.03
Supple. Table 5b.Percentage of Variability, Heritability and Genetic advance analysis for F3 Population in the intervarietal cross of (VBNGg 2 x KMG 189)
S.No / Plant Character / PCV % / GCV% / h2% / GA of Mean%1. / Plant height / 18.90 / 16.16 / 72.56 / 84.60
2. / No. of seeds per pod / 5.50 / 2.42 / 19.33 / 7.88
3. / No. of Pods per plant / 47.77 / 44.64 / 86.64 / 343.74
4. / Pod length / 31.93 / 31.91 / 99.91 / 133.60
5. / No. of productive branches / 9.69 / 7.30 / 56.00 / 35.24
Note:
S.No/Category / PCV and GCV / Heritability / GA of Mean1. / <10 – low / <30 - low / <10 – low
2. / 11-20 - Medium / 31 – 60 - Medium / 10-20 – Medium
3. / >20 - High / >60 - High / >20 - High
Suppl. Table 6. Correlation matrix for morphological traits and infectivity score studied in F2 genotypes of the cross VBN(Gg)2 * KMG189
Suppl. Table 7.MYMV Disease Scoring table for F2 generation
Serial Numer / Plant parts infected/disease (%) / Score / Disease reaction1 / No infection / 0 / Immune (I)
2 / 1-5 / 1 / Highly resistant (HR)
3 / 6-10 / 2 / Resistant (R)
4 / 11-20 / 3 / Moderately resistant (MR)
5 / 21-30 / 4 / Tolerant (T)
6 / 31-40 / 5 / Moderately Tolerant (MT)
7 / 41-50 / 6 / Moderately Susceptible (MS)
8 / 51-80 / 7 / Susceptible (S)
9 / 81-100 / 8 / Highly Susceptible (HS)
(Khan et al., 2007)
Suppl. Table 8. Distribution pattern of infectivity score among the 90 F2 genotypes of the cross VBN(Gg)2 * KMG189
Cross Combination / Number of F2 plants according to disease scoreMS – HS (3)
Moderately susceptible to highly susceptible / HR – MT (1)
Highly resistant to moderately tolerant
8 / 7 / 6 / 5 / 4 / 3 / 2 / 1
VBN (Gg) 2 x KMG 189 (F2)
Total 90 plants / 11 / 28 / 33 / 7 / 2 / 3 / 2 / 4
Suppl. Table 9. Polymorphic information content and marker index for parental survey ((VBNGg 2 and KMG 189) using RAPD marker
RAPD PRIMER / Total No. of Polymorphic bands / Total No. of Bands / PIC Value / MARKER INDEX (MI)OPBA 3 / 0 / 6 / 0.33 / 0
OPBA 4 / 0 / 0 / 0 / 0
OPBA 5 / 0 / 0 / 0 / 0
OPBA 6 / 0 / 6 / 0.33 / 0
OPBA 7 / 1 / 1 / 1 / 1
OPBA 8 / 1 / 1 / 1 / 1
OPBA 9 / 0 / 0 / 0 / 0
OPBA 10 / 0 / 8 / 0.25 / 0
OPBA 11 / 0 / 6 / 0.33 / 0
OPBA 12 / 0 / 0 / 0 / 0
OPBA 13 / 0 / 0 / 0 / 0
OPBA 16 / 0 / 4 / 0.5 / 0
OPBA 17 / 1 / 7 / 0.265 / 0.265
OPBA 18 / 0 / 6 / 0.33 / 0
OPBA 19 / 0 / 8 / 0.25 / 0
OPBA 20 / 0 / 6 / 0.33 / 0
OPBB 1 / 1 / 5 / 0.36 / 0.36
OPBB 2 / 0 / 2 / 1 / 0
OPBB 3 / 0 / 6 / 0.33 / 0
OPBB 4 / 0 / 4 / 0.5 / 0
OPBB 5 / 0 / 8 / 0.25 / 0
RAPD PRIMER / Total No. of Polymorphic bands / Total No. of Bands / PIC Value / MARKER INDEX (MI)
OPBB 6 / 0 / 4 / 0.5 / 0
OPBB 7 / 0 / 4 / 0.5 / 0
OPBB 8 / 0 / 4 / 0.5 / 0
OPBB 9 / 0 / 4 / 0.5 / 0
OPBB 10 / 0 / 4 / 0.5 / 0
OPBB 11 / 0 / 8 / 0.25 / 0
OPBB 12 / 0 / 4 / 0.5 / 0
OPBB 13 / 0 / 6 / 0.33 / 0
OPBB 14 / 2 / 8 / 0.219 / 0.438
OPBB 15 / 6 / 10 / 0.14 / 0.84
OPBB 16 / 0 / 10 / 0.2 / 0
OPBB 17 / 2 / 8 / 0.219 / 0.438
OPBB 18 / 0 / 6 / 0.33 / 0
OPBB 19 / 0 / 6 / 0.33 / 0
OPBB 20 / 1 / 13 / 0.148 / 0.148
OPBC 1 / 1 / 5 / 0.36 / 0.36
OPBC 2 / 0 / 15 / 0.129 / 0
OPBC 3 / 1 / 11 / 0.174 / 0.174
OPBC 4 / 4 / 8 / 0.172 / 0.688
OPBC 5 / 0 / 10 / 0.2 / 0
OPBC 6 / 5 / 19 / 0.091 / 0.455
OPBC 7 / 6 / 16 / 0.102 / 0.612
OPBC 8 / 0 / 12 / 0.168 / 0
OPBC 9 / 2 / 10 / 0.18 / 0.36
OPBC 10 / 0 / 10 / 0.2 / 0
OPBC 11 / 0 / 4 / 0.5 / 0
OPBC 12 / 0 / 6 / 0.33 / 0
OPBC 13 / 0 / 2 / 1 / 0
OPBC 14 / 0 / 4 / 0.5 / 0
OPBC 15 / 0 / 6 / 0.33 / 0
OPBC 16 / 0 / 8 / 0.252 / 0
OPBC 17 / 2 / 4 / 0.376 / 0.752
OPBC 18 / 6 / 10 / 0.14 / 0.84
OPBC 19 / 3 / 7 / 0.124 / 0.372
OPBC 20 / 2 / 10 / 0.18 / 0.36
OPBD 1 / 1 / 1 / 1 / 1
OPBD 2 / 1 / 7 / 0.266 / 0.266
OPBD 3 / 4 / 8 / 0.188 / 0.752
OPBD 4 / 0 / 2 / 1 / 0
OPBD 5 / 0 / 4 / 0.5 / 0
RAPD PRIMER / Total No. of Polymorphic bands / Total No. of Bands / PIC Value / MARKER INDEX (MI)
OPBD 6 / 4 / 10 / 0.16 / 0.64
OPBD 7 / 3 / 17 / 0.107 / 0.321
OPBD 8 / 0 / 6 / 0.33 / 0
OPBD 9 / 1 / 17 / 0.114 / 0.114
OPBD 10 / 1 / 9 / 0.21 / 0.21
OPBD 11 / 1 / 11 / 0.174 / 0.174
OPBD 12 / 0 / 10 / 0.2 / 0
OPBD 13 / 0 / 6 / 0.33 / 0
OPBD 14 / 2 / 4 / 0.375 / 0.75
OPBD 15 / 0 / 10 / 0.2 / 0
OPBD 16 / 0 / 8 / 0.25 / 0
OPBD 17 / 0 / 12 / 0.14 / 0
OPBD 18 / 0 / 10 / 0.2 / 0
OPBD 19 / 0 / 8 / 0.25 / 0
OPBD 20 / 0 / 10 / 0.2 / 0
OPBE 1 / 0 / 4 / 0.5 / 0
OPBE 2 / 0 / 10 / 0.2 / 0
OPBE 3 / 2 / 4 / 0.375 / 0.75
OPBE 4 / 2 / 6 / 0.278 / 0.556
OPBE 5 / 3 / 7 / 0.224 / 0.672
OPBE 6 / 0 / 12 / 0.167 / 0
OPBE 7 / 0 / 4 / 0.5 / 0
OPBE 8 / 0 / 6 / 0.33 / 0
OPBE 9 / 6 / 12 / 0.125 / 0.75
OPBE 10 / 1 / 9 / 0.21 / 0.21
OPBE 11 / 5 / 7 / 0.184 / 0.92
OPBE 12 / 1 / 5 / 0.36 / 0.36
OPBE 13 / 3 / 7 / 0.224 / 0.672
OPBE 14 / 0 / 10 / 0.2 / 0
OPBE 15 / 1 / 7 / 0.265 / 0.265
OPBE 16 / 0 / 2 / 1 / 0
OPBE 17 / 1 / 9 / 0.21 / 0.21
OPBE 18 / 1 / 3 / 0.55 / 0.55
OPBE 19 / 1 / 5 / 0.36 / 0.36
OPBE 20 / 0 / 4 / 0.5 / 0
Supple. Table 10.Polymorphic information content and Marker index for parental survey using ISSR primers
ISSR PRIMER / Total No. of Polymorphic bands / Total No. of Bands / PIC Value / MARKER INDEX (MI)UBC 834 / 2 / 10 / 0.18 / 0.36
UBC 811 / 0 / 10 / 0.2 / 0
UBC 812 / 1 / 7 / 0.265 / 0.265
UBC 836 / 2 / 8 / 0.219 / 0.438
UBC 857 / 0 / 10 / 0.2 / 0
UBC 841 / 0 / 12 / 0.167 / 0
UBC 847 / 0 / 6 / 0.33 / 0
UBC 856 / 1 / 11 / 0.174 / 0.174
UBC 855 / 0 / 8 / 0.25 / 0
UBC 825 / 2 / 10 / 0.18 / 0.36
UBC 808 / 0 / 12 / 0.167 / 0
UBC 826 / 2 / 4 / 0.375 / 0.75
UBC 884 / 1 / 13 / 0.148 / 0.148
UBC 848 / 1 / 9 / 0.21 / 0.21
UBC 846 / 2 / 6 / 0.278 / 0.556
UBC 889 / 1 / 9 / 0.21 / 0.21
UBC 835 / 1 / 11 / 0.174 / 0.174
UBC 815 / 3 / 9 / 0.185 / 0.555
Suppl. Table 11. Polymorphic information content and Marker index for Parents and Bulks using RAPD and ISSR primers
RAPD & ISSR / Total No. of Polymorphic bands / Total No. of Bands / PIC Value / MARKER INDEX (MI)OPBC 18 / 7 / 18 / 0.142 / 0.994
OPBC 4 / 7 / 14 / 0.138 / 0.966
OPBB 14 / 6 / 22 / 0.136 / 0.816
OPBB 15 / 4 / 17 / 0.19 / 0.76
OPBE 9 / 4 / 9 / 0.284 / 1.136
OPBE 11 / 5 / 14 / 0.204 / 1.02
OPBE 13 / 4 / 7 / 0.306 / 1.224
OPBE 18 / 2 / 7 / 0.429 / 0.858
UBC 855 / 2 / 16 / 0.227 / 0.454
UBC 848 / 5 / 20 / 0.16 / 0.8
UBC 835 / 5 / 19 / 0.169 / 0.845
UBC 815 / 5 / 15 / 0.191 / 0.955
Suppl. Table 12. Simple Sequence Repeats identified from 500Kb flanking regions of the SCAR CM-9 in chromosome 3.
Serial Number / Repeat sequence / No of repeats / Location Kb / Location from SCAR CM-91 / TA / 22 / 54 / Upstream
2 / AT / 18 / 85 / Upstream
3 / AT / 31 / 147 / Upstream
4 / AT / 21 / 201 / Upstream
5 / AT / 35 / 214 / Upstream
6 / TA / 41 / 246 / Upstream
7 / TA / 25 / 300 / Upstream
8 / AT / 66 / 387 / Upstream
9 / AT / 36 / 404 / Upstream
10 / TA / 25 / 464 / Upstream
11 / TA / 31 / 57 / Downstream
12 / TA / 26 / 86 / Downstream
13 / TA / 22 / 190 / Downstream
14 / AT / 29 / 235 / Downstream
15 / TA / 22 / 260 / Downstream
16 / TA / 40 / 275 / Downstream
17 / AT / 39 / 387 / Downstream
18 / TA / 47 / 401 / Downstream
19 / TA / 32 / 432 / Downstream
20 / AT / 34 / 482 / Downstream
Suppl. Fig 6. PCR analysis of SCAR CM-9 and CM-815 in ten other susceptible and resistant lines
Suppl. Table 13. Average infectivity percentage for MYMV in the Parental lines and F2 genotypes
S.No / Parents and F2 genotypes / Control / VA221 / VA 239R1 / R2 / R3 / R1 / R2 / R3 / R1 / R2 / R3
1. / VBN(Gg) 2 / 0 / 0 / 0 / 85% / 85% / 72% / 43% / 0 / 42%
2. / KMG 189 / 0 / 0 / 0 / 0 / 0 / 0 / 40% / 0 / 40%
3. / CM 15-5-6 (FR*) / 0 / 0 / 0 / 80% / 70% / 60% / 15% / 20% / 25%
4. / CM 15-3-8 (FR) / 0 / 0 / 0 / NG* / NG* / 20% / 0 / 0 / NG*
5. / CM 15-3-10 (FR) / 0 / 0 / 0 / 0 / 0 / NG* / 0 / 0 / 0
6. / CM 15-8-10 (FR) / 0 / 0 / 0 / 5% / 2% / 55% / 0 / 0 / 0
7. / CM 15-7-13 (FR) / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
8. / CM 15-2-9 (FR) / 0 / 0 / 0 / 0 / 0 / 0 / NG* / NG* / NG*
9. / CM 15-2-1 (FS*) / 0 / 0 / 10% / 72% / 78% / 45% / 12% / 15% / 10%
10. / CM 15-3-18 (FS) / 0 / 0 / 0 / 15% / 12% / 5% / 30% / 35% / 18%
11. / CM 15-2-3 (FS) / 0 / 0 / 0 / 0 / 0 / 0 / NG* / NG* / NG*
12. / CM15-5-23 (FS) / NG* / 0 / 0 / NG* / NG* / NG* / NG* / NG* / NG*
13. / CM 15-1-1 (FS) / 0 / 0 / 0 / 85% / 85% / 75% / 35% / 30% / 20%
14. / CM 14-2-12 (FS) / 0 / 0 / NG* / NG* / NG* / NG* / NG* / NG* / NG*
15. / CO 5 / 0 / 0 / 0 / 85% / 85% / 90% / 85% / 90% / 92%
*NG – NO GERMINATION, FR – FIELD RESISTANT LINE, FS – FIELD SUSCEPTIBLE LINE
Suppl. Fig 8a. Integrated density values based on standard sample values for KMG 189 parent at 30 cycles using Coat Protein Primer
Black is 0, White is 255
# IDV 30ng %* AREA AVG* BACK
1n -239 0.00 0.0 152 -1.57 35
2n 270 0.48 0.6 152 1.78 35
3n 624 1.12 1.3 152 4.11 35
4n 16819 30.11 35.6 152 110.65 35
5n 8824 15.80 18.7 152 58.05 35
6n 5067 9.07 10.7 152 33.34 35
7n 2221 3.98 4.7 152 14.61 35
8s 5586 10.00 11.8 168 33.25 0
9s 7811 13.98 16.5 168 46.49 0
IDV=Integrated Density Value s=standard
n=nonstandard a=approximated
* based on Standard Sample Value-Smooth Cubic Spline
Suppl. Fig 8b. Standard curve based on standard sample values for KMG 189 entry at 30 cycles
Suppl. Fig 9a. Integrated density values based on standard sample values for KMG 189 parent at 35 cycles using Coat Protein Primer
Black is 0, White is 255
# IDV 30ng %* AREA AVG* BACK
1n 1796 3.25 2.6 96 18.71 51
2n 2006 3.63 2.9 96 20.90 51
3n 2204 3.99 3.1 96 22.96 51
4n 18304 33.16 26.1 96 190.67 51
5n 13929 25.23 19.8 96 145.09 51
6n 10703 19.39 15.2 96 111.49 51
7n 779 1.41 1.1 96 8.11 51
8s 8281 15.00 11.8 140 59.15 0
9s 12232 22.16 17.4 140 87.37 0
IDV=Integrated Density Value s=standard
n=nonstandard a=approximated
* based on Standard Sample Value-Smooth Cubic Spline
Suppl. Fig 9b. Standard curve based on standard sample values for KMG 189 at 35 cycles
Suppl. Fig 10a.Integrated density values based on standard sample values for KMG 189 parent at 40 cycles using Coat Protein Primer
Black is 0, White is 255
# IDV 30ng %* AREA AVG* BACK
1n 2741 2.20 2.8 136 20.15 47
2n 2840 2.28 2.9 136 20.88 47
3n 4362 3.51 4.5 136 32.07 47
4n 22915 18.43 23.5 136 168.49 47
5n 18809 15.12 19.3 136 138.30 47
6n 15167 12.20 15.6 136 111.52 47
7n -1190 0.00 0.0 136 -8.75 47
8s 12436 10.00 12.8 189 65.80 0
9s 18136 14.58 18.6 189 95.96 0
IDV=Integrated Density Value s=standard
n=nonstandard a=approximated
* based on Standard Sample Value-Smooth Cubic Spline
Suppl. Fig 10b.Standard curve based on standard sample values for KMG 189 entry at 40 cycles
Suppl. Fig 11. Position of SCAR markers CM 9 and CM 815 in chromosome 3 of Vignaradiata