Supplementary Table 1. Sequences of the oligonucleotide primers used in this study.
Primera / Nucleotide sequence (5’-3’) / Annealing temperaturebp2 / AGC GAG TTT TCA TAC ACA AAT C / 45 oC
HC2+ / GCI GAR YTI CCI MGI ATW YTR GTK GAY CA
CP / CTG AGC TGT GTC TTT GAT GC / 54 oC
NIa-2 / CGT ACC TGC ACC TAA CG
NIa-1 / CTG TGA GGT GTT AAG TCC / 50 oC
P3-1 / CCC AGT TGT TCA AGG AG
P3-2 / GAT TTG TGA GTG CGT GC / 50 oC
5’CABMV / CTA GCT GGA CAC TAG TTG
GSP1-BR / TCT TAC CTC CCT CAT GCG GAA G / 54 oC
GSP2-BR / CAC TCT CAT ATT CAC CAG C
GSP1-MG / CAC TGT ATT GAG CTT GAG GG / 54 oC
GSP2-MG / CTC TCC AAA TGA TAC GCG GT
aPrimer HC2+ from reference [28]. All other primers designed during the course of this work.
bAnnealing temperature used for each primer pair during PCR amplification. Primer p2 was also used for reverse transcription using the standard protocol from the ThermoScript RT-PCR System (Invitrogen).
Supplementary Figure S1.Alignment of the deduced amino acid sequences of the polyproteins encoded by CABMV isolates BR1, MG-Avr andZ (AF348210). The polyprotein cleavage sites are indicated in red. Conserved motifs present throughout the polyprotein are indicated in blue. Amino acids which differ between the Brazilian isolates BR1 and MG-Avr are highlighted. Asterisks indicated amino acid positions which are conserved among the three sequences.
Supplementary Figure S2.Unrooted phylogenetic tree obtained from the deduced amino acid sequences of the complete polyprotein from the CABMV isolates BR1, MG-Avr and Z,plus26additional potyviruses. The tree was constructed using the neighbour-joining method with Poisson correction. The numbers at each branch correspond to bootstrap confidence levels (2000 replications). Vertical lengths are proportional to the genetic distances between the isolates. The darker shade indicates the monophyletic cluster comprising the three CABMV isolates. The ligher shade indicates the monophyletic cluster comprising the potyviruses from the BCMV subgroup. BCMNV, AY138897; BCMV, U34972;BYMV, U47033; BtMV, AY20639; ClYVV, AB011819; CABMV-Z, AF348210; DsMV, AJ298033; EAPV, AB246773; JGMV, Z26920; LMV, X97704;MDMV, AJ001691; OYDV, AJ510223;PLDMV, AB088221; PRSV, AY010722;PeMoV, AF023848; PepMoV, AF501591; PPV, X16415;PVY, X12456; PWV, HQ122652;SrMV, AJ310197; SMV, D00507; SCMV, AJ297628;TEV, M11458; TuMV, AF169561;YMV, U42596; ZYMV, AJ307036.
Supplementary Figure S1
BR1 MASIMIGTITVPFAWNECNYKEVDEYIETETQERVYMDPKPTS------ARKRNL 49
Z ------
MG-Avr MASIMFGDFLFTQIMEPAHEVVRTEEVITPVTKWVKQIVPVGCGTRCGGTVNYSKSSLRR 60
BR1 CFCGEDNDGHYHCHFC------DCECDSKNHLEEHERDICEDAYSVRAFGYKLVPKIE 101
Z ------
MG-Avr AIANGDLTRSGACHYCNTMGIVGGRVATEQLVAVTEQQVQKKEVKAVIPATTTTNWTLVD 120
BR1 IKKITKKV-PIVESSEPVHFTVRPVTIEAKHMASQKPSVRGELHRHRMESKKILSEYNRV 160
Z ------MIVDLKDEVEEAAKVIQIQKDHTSYTR------ESKKLLSDYDEA 39
MG-Avr KKLVDEGVRMIVDLKDEIADAARTIQTQKDHVSYTQ------ESKKLLSSYEKA 168
**: .: : :.: : : .* : : ****:**.*:..
BR1 QAAFADNFSKIAGMDQKLLVDKYSPIRKTKGGAVINRVSLKRAQERHEAQLMADKELAAF 220
Z QNKFTESLLQLPGMWAKLRIDAYSPIRKTKKGAIINRVSFRKAQERHEARLVADKELAAF 99
MG-Avr QAKFTENFFQLPGMRERLLVDTYSPIRETKKGVVINRVSFGEAQKRHEAQLMADNELAAF 228
* *::.: ::.** :* :* *****:** *.:*****: .**:****:*:**:*****
BR1 LAGEYESAAYIGSVQGLDTARNSESVSFKTPYYRRTPKIQKQKMKPKQVVGSSKVLGQVV 280
Z LAGEYESAAYIGSIQGLNTARNSESISFKTPYYRRTPKIQKQRIKPKQVVGSSQVLKQVM 159
MG-Avr LAGEYESAAYIGSVQGLDTIRNSESVSFKTPHYRRTPKIQKRRMKPKQVVGSLKVLEQVV 288
*************:***:* *****:*****:*********:::******** :** **:
BR1 QLAKQTGISLEFIEKGQGRTLKLNIVKRYGSALPKIILPHEGGKTVHEEVNCDKYKDTLL 340
Z QLAQQTGISVEFIERGKGRTLKVNVVKKYGSVLPKIILPHEEGVHVHEEVNYNKHKNTLL 219
MG-Avr QLAEQTGISMEFIGRGKGRTLKLNTVRKYGSVLPKIILPHEEGEKLHEEINYNKHKDTLL 348
***:*****:*** :*:*****:* *::***.********* * :***:* :*:*:***
BR1 SLIKHSTYKPIHQNVFKKGDSGLVYPTGKIKALPAHEKDVFVVRGRLDGTLINALDWCSE 400
Z FLIGHSSYKTIHQSVFRKGDSGLVYPTQKIQSLPEHDKEVFVVRGRLDGSLINALDWCSE 279
MG-Avr FLARHSSYKVIHQSVFKKGDSGLVYPTRVIEGLPEHDKDVFVVRGRSDGNLINALDWCNK 408
* **:** ***.**:********** *:.** *:*:******* **.********.:
BR1 PAHVQHYSHQPEVQFFQGWKKVFDKLVPRTQDHECTVDYSNEQCGELAASLSQILYPVKK 460
Z PSHVQHYSHQLEVQFFQGWKKVFDKLVPRTQDHECTVDYSNEQCGELAASLSQILYPVKK 339
MG-Avr PANVQHYSHQPEVQFFQGWKKVFDKLVPDTQDHECAIDYSNEQCGELAASLSQILYPVKK 468
*::******* ***************** ******::***********************
BR1 LSCRQCRFRIKDLSWEEYKEFVATHFGCCAEMLKEQQSVGFKNVQTLVERAVSEDGDMDL 520
Z LSCRQCRFRIKDLSWEEYKEFVATHFGCCAETLKEQQSVGFKNVQTLVERAVSEDGDMEL 399
MG-Avr LSCQQCRFRIKGSSWEEYKEFIATHFGRCAETLREQQSIGFKNVQTLVERAVSENGDMEL 528
***:*******. ********:***** *** *:****:***************:***:*
BR1 SLEIIRLTQNYTSTPMLQIQDINKALMKGSSASKQELDQALKQLLAMTQWWKNHMDLTGE 580
Z SLEIIKLTQNYTSTPMLQIQDINKALMKGSSASKQELDQALKQLLAMTQWWKNHMDLTGE 459
MG-Avr SLEIIKLTQNYTSTPMLQIQDINKALMKGSSASKQELDQALKQLLAMTQWWKNHMDLTGE 588
*****:******************************************************
BR1 DALKAFRNKRASKAILNPSLLCDNQLDKNGNFIWGERGRHSKRFFSNFFEEIIPSEGYSK 640
Z DALKTFRNKRASKAILNPSLLCDNQLDKNGNFIWGERGRHSKRFFSNFFEEIVPSEGYSK 519
MG-Avr DTLKTFRNKRASKAILNPSLLCDNQLDKNGNFIWGERGRHSKRFFSNFFEEVIPSEGYSK 648
*:**:**********************************************::*******
BR1 YTIRRNPNGQRKLAIGSLVVPLDLNRARVSMQGESVARNPLTKACVSILRKNFVYPCCCV 700
Z YTIRRNPNGQRKLAIGSLVVPLDLDRARVSMQGEGVARKPLTKACVSVLEKNFVYPCCCV 579
MG-Avr YTIRRNPNGQRKLAIGSLVVPLDLDRARVSMQGESVAKQPLTRACVSILEKNFVYPCCCV 708
************************:*********.**::***:****:*.**********
BR1 TLDNGQPLYSEYKSPTKRHLVVGSSGDPKYIDLPAADSDRMYIAKEGYCYLNIFLAMLVN 760
Z TLDNGQPLYSEYKSPTKRHLVVGSSGDPKYIDLPATDSDRMYIAKEGYCYLNIFLAMLVN 639
MG-Avr TLDNGQPLYSEYKSPTKRHLVVGSSGDPKYIDLPAADSNRMYIAREGYCYLNIFLAMLVN 768
***********************************:**:*****:***************
Suppl. Fig. S1 (cont.)
BR1 VNEEEAKDFTKMVRDVLVPKLGTWPTMMDVATAAYMLSVFHPETKSAELPRILVDHESQT 820
Z VNEEEAKDFTKMVRDVLVPKLGTWPTMMDVATAAYMLSVFHPETKSAELPRILVDHESQT 699
MG-Avr VNEEEAKDFTKMVRDVLVPKLGTWPTMMDVATAAYMLSVFHPETKSAELPRILVDHESQT 828
************************************************************
BR1 MHVIDSFGSLNTGYHVLKAGTVNQLIQFASNDLDSEMKFYKVGGSVQQRMKCETALITSI 880
Z MHVIDSFGSLNTGYHVLKAGTVNQLIQFASNDLDSEMKFYKVGGSVQQRMKCETALITSI 759
MG-Avr MHVIDSFGSLNTGYHVLKAGTVNQLIQFASNDLDSEMKFYKVGGIVQQRMKCETALITSV 888
******************************************** **************:
BR1 FKPKRMVHILETDPYILLMGLISPSLLIHMFRMRHLEKGVQIWINKEQSVAKIFLILEQL 940
Z FKPKRMVHILETDPYVLLMGLVSPSLLIHMFRMRHLEKGVQIWINKEQSVVKIFLILEQL 819
MG-Avr FKPKRMVHILETDPYVLLMGLVSPSLLIHMFRMRHLEKGVQIWINKEQSVAKIFLILEQL 948
***************:*****:****************************.*********
BR1 TKKLVVTDVLLEQLGLISETAEPLHSLLLNCPKTMHSYNIARELLTIKAEANSANEVLKL 1000
Z TKKLVVTDVLLEQLGLISEAAEPLHSLLLNCPNTMHSYNVARDLLTIKAEANSANEVLKL 879
MG-Avr TKKLVVTDVLLEQLGLISETAEPLHSLLLNCPKTMHSYNIARELLTIKAEANSANEVLKL 1008
*******************:************:******:**:*****************
BR1 NGFFDMNESLDSAREKIYVDRLSKEWHALSLLEKFSVTWRLKRFSDVTENTLTKKAFVDK 1060
Z NGFFDMNVSLDSAREKIYVERLSEEWHDLNLLEKFSVTWQLKRFSDVTENTLTKKATVDK 939
MG-Avr NGFFDMNESLDSAREKIYVDRLSKEWHALSLLEKFSVTWRLKRFSDVTENTLTKKAFVDK 1068
******* ***********:***:*** *.*********:**************** ***
BR1 EKFSRRFVSACFLSAQTHLKESRTYIANRVEQFSLKLVSSVCNMFLKFVRKCYGDLIFLV 1120
Z EKFSKRFVSACFMSAQTHLRESRIYLANKVEQFSHRLIGSVCNMFLRFVRRCYGDLIFLV 999
MG-Avr EKFSRRFVSACFLSAQTHLKESRTYIANRVEQFSLKLVSSVCNMFLKFVRKCYGDLIFLV 1128
****:*******:******:*** *:**:***** :*:.*******:***:*********
BR1 NVSIVFSLFVQMISTLNSTMRAIKNDKISLAAHKREKDEQTICRMYDLFIKGSKDLPSSG 1180
Z NVSIVFSLFIQMISILNSTMSAIRKDRISLAAHKREKDEQTICRMYDLFIKGSKDLPSAG 1059
MG-Avr NVSIVFSLFVQMISTLNSTMRAIKNDKISLAAHKREKDEQTICRMYDLFIKGSKDLPSSG 1188
*********:**** ***** **::*:*******************************:*
BR1 QFLSHVEEVRPDLLLTAQYMIADREHV-ETQAKTHNQVHLEKVVAFMALLTMCVDAEGSD 1239
Z QFLSHVEEFRPDLLLTAQYMITDHGRVGRHQAKTHSQVHLEKIVAFMALLTMCVDAERSD 1119
MG-Avr QFLSHVEEVRPDLLLTAQYMIADREHV-ETQAKTHNQVHLEKVVAFMALLTMCVDAERSD 1247
********.************:*: :* . *****.******:************** **
BR1 AIFKILNKLKSVFGTMAEEVRMQSLDDLNDIEECKKLTVDFEVSTSYEPTSTIIDVSFEG 1299
Z AIFKILNKLKSVFGTMAEEVRVQGLDDLNDIEECKKLTVDFEVSTSYEPTSTTIDVSFEG 1179
MG-Avr AIFKILNKLKSVFGTMAEEVRMQSLDDLNDIEECKKLTVDFEVSTSYEPTSTIIDVSFEG 1307
*********************:*.**************************** *******
BR1 WWNRQLQQNRVVPHYRSTGEFMEFTRATAAQVANSVSLSAQNDFLIRGAVGSGKSTGLPH 1359
Z WWNRQLQQNRVVPHYRSTGEFMEFTRATAAQVANSISLSAQNDFLIRGAVGSGKSTGLPH 1239
MG-Avr WWNRQLQQNRVVPHYRSTGEFMEFTRATAAQVANSVSLSAQNDFLIRGAVGSGKSTGLPH 1367
***********************************:************************
BR1 HLAKKGKVLLLEPTRPLAENVSKQLSQEPFFHNVTLRMRGLSRFGSSNITVMTSGLPFHY 1419
Z HLAKKGKVLLLEPTRPLAENVSKQLSQEPFFQNVTLRMRGLSRFGSSNITVMTSGFAFHY 1299
MG-Avr HLAKKGKVLLLEPTRPLAENVSKQLSQEPFFHNVTLRMRGLSRFGSSNITVMTSGFAFHY 1427
*******************************:***********************:.***
BR1 YVNNPHQLNGFDYIIMDECHVLDSSSIAFNCALKEFSYAGKLIKVSATPPGRECEFTTQY 1479
Z YVNNPHQLNDFDFIIMDECHVLDSSSIAFNCALKEFSYAGKLIKVSATPPGRECEFTTQH 1359
MG-Avr YVNNPHQLNGFDYIIMDECHVLDSSSIAFNCALKEFSYAGKLIKVSATPPGRECEFTTQY 1487
*********.**:**********************************************:
BR1 PVKLKIEESLSFQDFVQAQGTGTNADMIQHGSNLLVYVASYNEVDQLATYLLNKNYKVTK 1539
Z PVKLKVEENLSFQDFVQAQGTGTNADMVQHGSNLLVYVASYNEVDQLAAYLLNKNYKVTK 1419
MG-Avr PVKLKIEESLSFQDFVQAQGTGTNADMIQHGSNLLVYVASYNEVDQLATYLLNKNYKVTK 1547
*****:**.******************:********************:***********
BR1 VDGRTMQMGRVEIETSGNPSKPHFIVATNIIENGVTLDVDCVIDFGLKVVADLDTDSRCV 1599
Z VDGR--TMGRVEIETSGNPAKPHFIVATNIIENGVTLDVDCVIDFGLKVVADLDTDSRCV 1477
MG-Avr VDGRTMQMGRVEIETSGNPSKPHFIVATNIIENGVTLDVDCVIDFGLKVVADLDTDSRCV 1607
**** ************:****************************************
Suppl. Fig. S1 (cont.)
BR1 RYNKKSVNYGERIQRLGRVGRHKPGFALRIGSTERGLTEIPDFIATEAAFLSFAYGLPVT 1659
Z RYNKKNVSYGERIQRLGRVGRHKPGFALRIGNTERGLTEIPEFIATEAAFLSFAYGLPVT 1537
MG-Avr RYNKKSVNYGERIQRLGRVGRHKPGFALRIGSTERGLTEIPDFIATEAAFLSFAYGLPVT 1667
*****.*.***********************.*********:******************
BR1 TQNVTTNILSKCTVQQAKSALNFELTPFFTTHLVKYDGSIHPEIHKLLKPFKLRESEMIM 1719
Z TQNVTTNILSKCTVQQAKNALNFELTPFFTTHFVKYDGSMHPEIHKLLKPFKLRESEMVM 1597
MG-Avr TQNVTTNILSKCTVQQAKSALNFELTPFFTTHLVKYDGSIHPEIHKLLKPFKLRESEMIM 1727
******************.*************:******:******************:*
BR1 NKKAIPYQYVNQWISVREYRRLGIQIGCDERVQLPFYVNGIPDKLFEALWETVSKYRYDA 1779
Z NKKAIPYQYVNQWISVKEYKRLGIQIGCDERVQLPFYVNGIPDKLFEALWETVSKYRYDA 1657
MG-Avr NKKAIPYQYVNQWISVREYRRLGIQIGCDERVQLPFYVNGIPDKLFEALWETVSKYRYDA 1787
****************:**:****************************************
BR1 GFGRISSASSTKISYTLSTEPTAIPRTIAIIDHLISEEMMKKNHFDTVASSLTGHSFSLS 1839
Z GFGRISSASSTKISYTLSTEPTAIPRTIAIIDHLISEEMMKKNHFDTVASSLTGYSFSLS 1717
MG-Avr GFGRISSASSTKISYTLSTEPTAIPRTIAIIDHLISEEMMKKNHFDTVASSLTGHSFSLS 1847
******************************************************:*****
BR1 GIAEGIRKRYLRDYSTQNIETLQQARSQLLEFNSNTVDVNKLHEYEDLGILNTVCLQSKQ 1899
Z GIAEGIRKRYLRDYSTQNIETLQQARSQLLEFNSNTVDVNKLPEYEDLGILNTVCLQSKH 1777
MG-Avr GIAEGIRKRYLRDYSTQNIETLQQARSQLLEFNSNTVDVNKLHEYEDLGILNTVCLQSKQ 1907
****************************************** ****************:
BR1 EVAKYLGLKGKWDGSRLRNDLLLVIFTIIGGGWMMWDYFSRCTQEEVTTQGKKRMLQKLK 1959
Z EVAKYLGLKGKWDGSKFRNDFLLVVFTIIGGGWMMVDYFSKCTQEEVTTQGKKRMMQKLK 1837
MG-Avr EVAKYLGLKGKWDGSRLRNDLLLVIFTIIGGGWMMWDYFSRCTQEEVTTQGKKRMLQKLK 1967
***************::***:***:********** ****:**************:****
BR1 FRDAHDRKVGREVYADDYTMEHTFGEAYTKKGKEKGSHKTKGMGRKTRNFIHMYGVEPEN 2019
Z FRNARDRKVGREVYADDYTMEHTFGEAYTKKGKEKGSHKTKGMGRKTRNFIHMYGVEPEN 1897
MG-Avr FRDAHDRKVGREVYADDYTMEHTFGEAYTKKGKEKGSHKTKGMGRKTRNFIHMYGVEPEN 2027
**:*:*******************************************************
BR1 YATIRFVDPLTGFTMDENPKVDIRLVQDEIGDVRNKFMDEGKLDRQAIMYNPGVQAYFLG 2079
Z YSTIRFVDPLTGFTMDEHPRVDIRIVQDEIGEVRGKLMDEGELDRQSIKHNPGIQAYFFG 1957
MG-Avr YATIRFVDPLTGFTMDENPKVDIRLVQDEIGDVRNKFMDEGKLDRQAIMYNPGVQAYFLG 2087
*:***************:*:****:******:**.*:****:****:* :***:****:*
BR1 RGTEKALKVDLTPHRPTLLCAHSNNIAGYPEREDELRQTGLPQEVDLKDVPAPNEDVGVE 2139
Z KGTEKALKVDLTPHRPTLLCMHSNNIAGYPERENELRQTGLPQEIDLKDVPAPNEDVGVE 2017
MG-Avr RGTEKALKVDLTPHRPTLLCAHSNNIAGYPEREDELRQTGLPQEVDLKDVPAPNENVGVE 2147
:******************* ************:**********:**********:****
BR1 SKSTYKGPRDYSGISTLVCKIVNASDGCTETIFGIGYGSYIITNGHLFKRNNGTLTVKTW 2199
Z SKSTYKGPRDYSGISTLICKIVNASDGCTETIFGIGYGSYIITNGHLFKRNNGTLTVKTW 2077
MG-Avr SQSTYKGPRDYSGISTLVCKIVNASDGCTETIFGIGYGSYIITNGHLFKRNNGTLTVKTW 2207
*:***************:******************************************
BR1 HGEFVVSNTTQLRIHFIEGKDAILIRVPKDFPPFAQRNCFRSPKKEERVCMIGTNFQDKS 2259
Z HGEFVVSNTTQLKIHFIEGKDAILIRMPKDFPPFAQRNCFRSPKKEERVCMIGTNFQEKS 2137
MG-Avr HGEFVVPNTTQLRIHFIEGKDAILIRMPKDFPPFAQKNCFRSPKKEERVCMVGTNFQDKS 2267
******.*****:*************:*********:**************:*****:**
BR1 LRSTVSESSMVIPEGKGSFWVHWISTQDGDCGLPLVSVDDGHIVGFHGLASNTTSRNFFV 2319
Z LRSTVSESSMVIPEGKGSFWVHWISTQDGDCGLPLVSVDDGHIVGFHGLASNTTSRNFFV 2197
MG-Avr LRSTVSESSMVIPEGKGSFWIHWISTQDGDCGLPLVSVNDGHIVGFHGLASNTTSRNFFV 2327
********************:*****************:*********************
BR1 PFIDGFKEKYLDCAETLEWNRHWLWQPDKIAWGSLNLVNNQPKEEFKIAKLITDLFDDGV 2379
Z PFIDGFKEKYLDCAETLEWNRHWLWQPDKIAWGSLNLINNQPKEEFKIAKLITDLFDDRG 2257
MG-Avr PFIDEFKEKYLDCAETLEWNKHWLWQPDKIAWGSLNLVSNQPKEEFKIAKLITDLFDDGV 2387
**** ***************:****************:.*******************
BR1 ATQSKQEAWLRNAMEGNLVACGKAESALVTKHVVKGKCCYFQQYLSSNQSAADFFKPLMG 2439
Z CTQSKQEAWLRSAIEGNLIACGKAESALVTKHVVKGKCSYFQQYLGSNQSAADFFKPLMG 2317
MG-Avr ATQSKQEAWLRNAMEGNLVACGKAESALVTKHVVKGKCRYFQQYLSSNQSAADFFKPLMG 2447
.**********.*:****:******************* ******.**************
Suppl. Fig. S1 (cont.)
BR1 FYQPSRLNKEAFKKDFFKYNKPVVVGEVDFDAFAQAVSGVKMMMIEFGFGECKYVTDSEE 2499
Z FYQPSRLNREAFKKDFFKYNKPVTVGKVDFYAFMQAVNGVKMMMIEFCFSECKYVTDSRR 2377
MG-Avr FYQPSRLNKEAFKKDFFKYNKPVVVGEVDFDAFERAVSEVKMMMNEFGFSECKYVTDSEE 2507
********:**************.**:*** ** :**. ***** ** *.********..
BR1 IF-DSLNMKAAVGAQYKGKKQDYFATMDEFDRERLVYLSCERLFYGKKGLWNGSLKAELR 2558
Z NFPDSLNMKAAVGAQYKGKKQDYFATMDKFDRERLVYLSCERLFHGKKGLWNGSLKAELR 2437
MG-Avr IF-DSLNMKAAVGAQYKGKKQDYFATMDKFDRERLVYLSCERLFYGWKGLWNGSLKAELR 2566
* *************************:***************:* *************
BR1 PLEKVEANKTRTFTAAPIDTLLGAKVCVDDFNIHFTNSNLQCPWTVGMTKFYGGWDKLMR 2618
Z PLEKVEANKTRTFTAAPIDTLLGAKVCVDDFNNQFYNFNLQCPWTVGMTKFYGLGDKLMR 2497
MG-Avr PLEKVEANKTRTFTAAPIDTLLGAKVCVDDFNNQFYNFNLQCPWTVGMTKFYGGWDKLMR 2626
******************************** :* * *************** *****
BR1 SLPEGWVYCHADGSQFDSSLTPLLLNAVLDLRLFFMEDWWVGQEMLENLYAEIVFTPILT 2678
Z SLPEGWIYCHADGSQFDSSLTPLLLNAVLDLRMFFMEDWWVGQEMLENLYAEIVFTPILT 2557
MG-Avr SLPEGWVYCHADGSQFDSSLTPLLLNAVLDLRLFFMEDWWVGQEMLENLYAEIVFTPILT 2686
******:*************************:***************************
BR1 PDGTVVKKFRGNNSGQPSTVVDNTLMVVISVYYSCIKAGWNEADIQERLVFFANGDDIIL 2738
Z PDGTVVKKFRGNNSGQPSTVVDNTLMVVISVYYSCIKAGWNEVDIQERLVFFANGDDIIL 2617
MG-Avr PDGTVVKKFRSNNSGQPSTVVDNTLMVVISVYYSCIKAGWNEADIQERLVFFANGDDIIL 2746
**********.*******************************.*****************
BR1 AAQEKDIGILDTFTKSFKELGLNYDFSERTRRREELWFMSHQAKLVGDLYIPKLEQERIV 2798
Z AAQEKDIGILDTFTKSFKELGLNYDFSERTKKREELWFMSHQAKLVGDLYIPKLEQERIV 2677
MG-Avr AAQEKDIGILDTFTKSFKELGLNYDFSERTRKREELWFMSHQAKLVGDLYIPKLEQERIV 2806
******************************::****************************
BR1 SILEWDRSKEMLHRTEAVCAAMIEAWGYPELLQEIRKFYLWLLQRDEFKELASLGKAPYI 2858
Z SILEWDRSKEMLHRTETVCAAMIEAWGYPELLQEIRKFYLWLLQRDEFKELASLGKAPYI 2737
MG-Avr SILEWDRSKEMRHRTEAVCAAMIEAWGYPELLQEIRKFYLWLLQRDEFKELARLGKAPYI 2866
*********** ****:*********************************** *******
BR1 AETALKKLYTDEQASEKELQRYLQDILSFYDDCESEDVVLQSDEKQKELDAGKDKDKEKE 2918
Z AETALKKLYTDEQASEKELQRYLQDILSFYDDSESEDVVLQSDERQKELDAGKDKDKAKE 2797
MG-Avr AETALKKLYTDEQASEKELQRYLQGILSFYDDCESEDVVLQSDGKDKELDAGKDKDKVKE 2926
************************.*******.********** ::*********** **
BR1 AKGQSAQQKQAKNKGTKETERDVANSSSGQLVPRLQKISKKMNLPMIAGKVILNLDHLIE 2978
Z AREQSTQQKQAKNKGAKETERDVAASSSGQLVPRLQKISKKMNLPMVAGRLILNIDHLIE 2857
MG-Avr AKEQSVQQKQAKNKGAKETERDVATSSSGQLVPRLQKISKKMNLPMVAGRVLLNLDHLIE 2986
*: **.*********:******** *********************:**:::**:*****
BR1 YKPAQIDLYNTRASKTQLSKWFEAIKEEYELDDDKMGVIMNGFMVWCIENGTSPDINGVW 3038
Z YKPKQIDLYNTRASKAQFNTWFEAVKEEYELDDDKMSVIMNGFMVWCIENGTSPDVNGVW 2917
MG-Avr YKPAQIDLYNTRASKTQLSKWFEAIKGEYELDDDKMGVIMNGFMVWCIENGTSLDVNGVW 3046
*** ***********:*:..****:* *********.**************** *:****
BR1 TMMDGDEQVEFPLKPIVENAKPTLRQIMHHFSDAAEAYIEMRNSEGFYMPRYGLLRNLRD 3098
Z TMMDGDEQVEFPLKPIVENAKPTLRQVMHHFSDAAEAYIEMRNSEGFYMPRYGPLRNLRD 2977
MG-Avr TMMEGDQAVEFSLNPLVGYRKPPLLAIMHLFSNAPEAYLEMGNSGRLLTPRSGLLGNLKD 3106
***:**: ***.*:*:* **.* :** **:*.***:** ** : ** * * **:*
BR1 KSLARYAFDFYEVTSKTSERAREAIAQMKAAALANVNTRMFGLDGNVATTSENTERHTAA 3158
Z KSLARYAFDFYEVTSKTSDRAREAIAQMKAAALANVNTRMFGLDGNVATVSENTERHTAA 3037
MG-Avr KSLARYAFDFYEVTSKTSDRAREAIAQMKAAALANVNTRMFGLDGNVATTSENTERHTAT 3166
******************:******************************.*********:
BR1 DVNQNMHSLLGMTHGQ 3174
Z DVNQNMHSLLGMTHGQ 3053
MG-Avr GVNQNMHSLLGMTHGQ 3182
.***************
Supplementary Figure S2