Course on Molecular Diagnostics (X)
Thursday 19Friday 20 May,2016
vs 160428
The ErasmusMedicalCenter houses many different molecular diagnostic techniques, which are subject to continuous research and are developed based on their practical applications. These applications are based on all areas of the healthcare, such as Clinical Genetics, Infectious Diseases, Complex Diseases and a broad spectrum of oncological adaptations.
This course will bring the state-of-the-art of these areas of the Molecular Diagnostics, and is meant for translational researchers as well as doctors and medical specialists operating outside of their initial field of research. But also PhD-students and technicians are welcome. This course is a yearly updated version of the first issues organized from 2007 on. It will be given in English.
The course starts with 3 presentations on basic molecular techniques:
- PCR and real-time PCR;
- DNA sequencing; basic technologies;
- Next generation Sequencing (NGS);
followed by 1,5 day of applications in various fields
For last year’s courses, a book on Molecular Diagnostics was written by most of the teachers of the course (edited by Dr. W. van Leeuwen and dr. C. Vink; IVA editors, 2009). This book is advised for the course and can be bought at the start of the course for the reduced price of €45 (normal price €65,45).
The course will take place in room Ae-406.Full participation will be awarded with 1 ECTS.For more information: The maximum amount of participants is 70. If you have any questions, please call Frank van Vliet (010-408 7518 or 06-5474 6408).
Organising committee:
Dr. Erik-Jan Dubbink, dept. of Pathology
Dr. Wilfred van IJcken, Biomics Core Facility
Dr. Corné Klaassen, Dept. Of Microbiology
Dr. Ton Langerak, Dept. of Immunology
Dr. Suzan Pas, Dept. of Viroscience
Dr. Peter Valk, Dept. of Hematology
Dr. Frank van Vliet, managing director Postgraduate School MolMed
Thursday 19 May 2016, room Ae-406
Morning session: Techniques
Moderator: Suzan Pas, Dept. of Virology, Erasmus MC
Time / Speaker / Title / Keywords09.00-9.30 / Registration & coffee in room Ae-406
09.30-9.45 / Suzan Pas, Dept. of Viroscience, Erasmus MC / Opening, introduction
09.45-10.30 / Suzan Pas, Dept. of Viroscience, Erasmus MC / PCR, real-time PCR and new techno-logies / Both PCR and real-time PCR; dual probe/ dual target; Taqman-techniques; MGB & LNA probes; new technologies like Digital PCR, etc.
10.30-11.00 / Lida Prins,Dept. of Clinical Genetics, Erasmus MC / DNA sequencing; basic technologies / Sanger dideoxy, pyrosequencing
11.00-11.15 / Coffee break
11.15-12.00 / Wilfred van IJcken, Biomics Core Facility / Next generation Sequencing (NGS) / High Throughput Sequencing, DNA & RNA Sequencing; overview of techniques, 1st/ 2nd/ 3rd generation techniques
12.00-12.15 / Wilfred van IJcken(chair), Suzan Pas, Lida Prins / Q&A with the audience; all questions will be answered! / ‘There are no stupid questions, only stupid answers’
12.15-13.00 /
Lunch break
Afternoon session: ApplicationsModerator: Corné Klaassen, Dept. of Microbiology
13.00-13.40 / Jeroen Knijnenburg,Dept. of Clinical Genetics, Erasmus MC(alt.: Femke de Vries) / Molecular (cyto) genetics and diagnostics of congenital defects / Karyotyping,FISH ,whole genome analysis,copy number variation13.40-14.20 / Suzan Pas, Dept. of Virology, Erasmus MC / Application of molecular techniques in virus diagnostics / Patient management; viral pathogenesis; quantitative (RT-)PCR; virus typing; Point of impact testing
14.20-14.35 / Coffee break
14.35-15.15 / Joyce van Meurs, Dept. of Internal Medicine, Erasmus MC / High throughput genotyping techniques
15.15-15.20 / Short break / 15.45-16.00 / Coffee break
15.20-16.00 / Leendert Looijenga, Dept. of Pathology, Erasmus MC / Molecular Diagnosis of Human Germ Cell Cancer / SNPs and miRNAs as novel biomarkers
16.00-16.40 / Ron van Schaik, Dept. of Clinical Chemistry, Erasmus MC / Pharmacogenetics of CYP450 enzymes / Pharmacogenetics: cytochrome P450, SNPs, personalized medicine
Friday 20 May2016, room Ae-406
Morning session: Applications (continued)
Moderator: Peter Valk, Dept. of Hematology, Erasmus MC
Time / Speaker / Title / Keywords09.00-09.10 /
Coffee
09.10-09.50 / Peter Valk, Dept. of Hematology, Erasmus MC / RNA expression microarray analysis / High throughput gene expression arrays09.50-10.30 / Kim van Kessel, Dept. of Pathology, Erasmus MC / Assays for diagnostics and treatment selection in bladder cancer; cost-effectivess study in bladder cancer diagnostics / Bladder cancer, urine surveillance, treatment selection & cost-effectivess in diagnostics
10.30-10.45 /
Coffee break
10.45-11.25 / Ton Langerak/ Vincent van der Velden, Dept. of Immunology, Erasmus MC / Molecular diagnostics of lymphatic malignancies / Clonality assessment, somatic hypermutation analysis, minimal residual disease analysis11.25-12.05 / Wim Dik, Dept. of Immunology, Erasmus MC / HLA typing and screening for clinical transplantation purposes / HLA, gene polymorphism, transplantation, DNA typing, PCR, dotblot, sequencing
12.05-12.45 / Corné Klaassen, Dept. of Microbiology, Erasmus MC / Diagnostics in medical microbiology & classification of MRSA bacteria with Raman spectro-scopy; mass spec / Molecular bacteriology, - parasitology, and – mycology; identification and characterization; mass spectroscopy
12.45-13.30 / Lunch break
Afternoon session: Applications
Moderator: Ton Langerak, Dept. of Immunology, Erasmus MC
13.30-14.10 / Winand Dinjens, Dept. of Pathology, Erasmus MC / Pathology of solid tumors / Molecular diagnostics of solid tumors14.10-14.50 / Ans van den Ouweland, Dept. of Clinical Genetics, Erasmus MC / UV (Unclassified Variants): SNP or pathogenic mutation / Examples: Tourette, BRCA, …
14.50-15.05 / Coffee break
15.05-15.45 / Miranda de Graaf, Dept. of Virology, Erasmus MC / Phylogeny in infectious diseases / An introduction to phylogeny. Evolution of RNA and DNA viruses
15.45-16.25 / Pieter Fraaij, Dept.of Virosciences, Erasmus MC / Casuistics: clinical cases / Genotyping, outbreaks, molecular epidemiology, patient care, infection prevention
Attendance fees
The subscription fee of non-commercial participants for the Course is €400.
On top of that,the book on Molecular Diagnostics, written by most of the teachers of the course (Edited by Dr. W. van Leeuwen and dr. W. Vink;IVA editors, 2009) is advised. Almost all of the materials of the course are in this book. This book can be bought and paid in cash upon arrival. When you already have one, please bring it with you to the course!
Discounts are handled as followed:
- All PhD students and Master of Science students get 50% discount and pay €200+ €45 for the bookupon arrival;
- All participants from the postgraduate school MolMed get a discount of 100% and pay €0 + €45 for the book upon arrival.
- Master of Science students who pay private get a discount of 75% and pay €100.
If these financial requirements pose a problem but you wish to attend the Course, please contact Frank van Vliet, managing director of the Erasmus Postgraduate School Mol Med, at: .
Invoice
Fees can be paid upon an INVOICE. After your registration you will receive the INVOICE per mail. This can take a while. Payment per bank can be done together with the number on your INVOICE.
Late participants can also pay in cash upon signing in for the Course.
Cancellations
The fees are for all the days of the Course. There is no discount for partaking only in part of the Course. Our cancellation policy is that cancellation is possible up to one week before the start of the Course. Later cancellation will not be accepted, but you are allowed to send a substitute.
Commercial participants & sponsors
Companies are invited to inquire for participation and sponsoring.
1