Supplemental Materials

Supplemental Table 1. Historical Maui Parrotbill museum sample information from American Museum of Natural History, New York, USA (AMNH), Museum of Comparative Zoology, Cambridge, MA, USA (MCZ), Cambridge University Museum of Zoology, Cambridge, UK (CMZ), British Museum of Natural History, London, UK (BMNH UK), Bernice Pauahi Bishop Museum, Honolulu, USA (BMNH US), and Smithsonian National Museum of Natural History, Washington DC, US (USNM). Museum specimens were collected across the period from 1892-1901. All specimens were presumed to be collected from the western-most region of the parrotbill’s range on Haleakala as per collectors’ notes on locations.

Museum / Sample ID / Date of Collection / Collector / Location / Age / Sex
AMNH / 193408 / 05/1896 / Perkins / Haleakala / AHY / Male
AMNH / 193409 / 6/17/1901 / Henshaw / Olinda / AHY / Female
AMNH / 453556 / 10/1896 / Palmer / Haleakala / AHY / Male
AMNH / 453557 / 08/4/1892 / Palmer / AHY / Male
AMNH / 453558 / 08/3/1892 / Palmer / AHY / Male
AMNH / 453559 / 08/16/1892 / Palmer / AHY / Male
AMNH / 453560 / 08/15/1892 / Palmer / AHY / Female
AMNH / 453561 / 08/3/1892 / Palmer / AHY / Female
AMNH / 453562 / 08/03/1892 / Palmer / AHY / Unknown
AMNH / 453563 / Palmer / AHY / Female
MCZ / 134719 / 1894 / Perkins / AHY / Male
MCZ / 47905 / 1896 / Perkins / AHY / Male
CMZ / 27/DRE/9/a/1 / 1894 / Perkins / AHY / Female
CMZ / 27/DRE/9/a/2 / 1896 / Perkins / AHY / Male
CMZ / 27/DRE/9/a/3 / 1896 / Perkins / AHY / Female
CMZ / 27/DRE/9/a/4 / 1896 / Perkins / HY / Male
CMZ / 27/DRE/9/a/5 / 1896 / Perkins / SY / Male
BMNH UK / 1939.12.9.53 / 1892 / Rothschild / AHY / Male
BMNH UK / 1939.12.9.57 / 08/05/1892 / Rothschild / HY / Unknown
BMNH US / 95.7.20.170 / 1894 / Perkins / AHY / Male
BMNH US / 95.7.20.171 / 1894 / Perkins / AHY / Female
BMNH US / 97.10.28.22 / 1896 / Perkins / AHY / Female
BMNH US / 97.10.28.23 / 1896 / Perkins / AHY / Male
BMNH US / 97.10.28.24 / 1896 / Perkins / SY / Female
BMNH US / 97.10.28.25 / 1896 / Perkins / AHY / Male
USNM / 177971 / 1901 / Henshaw / Ukulele / AHY / Male
USNM / 177972 / 1901 / Henshaw / Ukulele / AHY / Female

Supplemental Table 2. Average allelic and private allelic richness using rarefaction technique over all loci. Includes results for 10, 20, 50, and 110 genes based on microsatellite data across the east (n=110), west (n=19), and captive (n=11) Maui Parrotbill (Pseudonestor xanthophrys) populations.

Allelic Richness / 10 genes / 20 genes / 50 genes / 110 genes
Captive / 3.27 / 3.82 / 3.83 / 3.83
East / 3.74 / 4.71 / 5.83 / 6.66
West / 3.04 / 3.72 / 4.08 / 4.08
Private Allelic Richness
Captive / 0.47 / 0.28 / 0.09 / 0.00
East / 0.71 / 0.89 / 1.47 / 2.10
West / 0.45 / 0.43 / 0.35 / 0.26

Supplemental Figure 1. Chromosome locations in the zebra finch (Taeniopygia guttata) genone of the 12 microsatellite loci characterized in the Maui Parrotbill (Pseudonestor xanthophrys) compiled by the National Library of Medicine, http://www.ncbi.nlm.nih.gov/blast/ Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=OGP__59729__12898).

Additional Materials

Black and white illustration of Figure 3 for printable version.

Figure 3. Maui Parrotbill (Pseudonestor xanthophrys) assignment probabilities to each population cluster from GENELAND results of K=2 (right) and K=3 (left).