Supplementary Table 2. Primers for Testing Expression Levels of Metabolic Marker Genes

Supplementary Table 1. Composition of hydrogenated coconut, corn and olive oil diets. All diets are the low sucrose formulations.

Diet / Fat Source / Research Diet # / Fatty Acid Composition (%) / Fat (%) / Carbohydrate (%) / Protein (%)
5010 / Crude / NA / NA / 13.5 / 59.0 / 27.6
LF / Coconut / D12328 / Lauric (47.6), Myristic (18.0), Stearic (10.6), Other (23.8) / 10.5 / 73.1 / 16.4
HF / Coconut / D12330 / 58.0 / 25.5 / 16.4
LF / Corn / D08121801 / Linoleic (60.1), Oleic (24.9), Palmitic (10.9), Other (4.1) / 10.5 / 73.1 / 16.4
HF / Corn / D09053101 / 58.0 / 25.5 / 16.4
LF / Olive / D10111601 / Oleic (70.5), Linoleic (13), Palmitic (11.5), Other (5) / 10.5 / 73.1 / 16.4
HF / Olive / D10111602 / 58.0 / 25.5 / 16.4

Supplementary Table 2. Primers for testing expression levels of metabolic marker genes.

Supplementary Table 3. B6 and CSS strains after 60 days on the coconut diet: Final body weight (FBW), epididymal fat pad mass (EFPM), body mass index (BMI), fasting insulin, fasting glucose, HOMA-IR and total polyp number. Grey shaded areas denote mice fed the LFDCoco. For statistical analysis, each strain fed the HFDCoco was compared to the same strain fed the LFDCoco. * P<0.01, ** P<0.001, *** P<0.0001.

Strain / N / FBW (g) ± SEM / EFPM (g) ± SEM / BMI ± SEM / Fasting Insulin (µg/L) ± SEM / Fasting Glucose (mg/dL) ± SEM / HOMA-IR / Total Polyp Number
B6.Apc+/+ / 54 / 27.91 ± 0.24 / 0.61 ± 0.12 / 0.24 ± 0.008 / 0.34 ± 0.14 / 92.4 ± 4.2 / 20.8 ± 12.6 / 0.0 ± 0.0
B6.ApcMin/+ / 54 / 25.78 ± 0.24 / 0.32 ± 0.04 / 0.25 ± 0.002 / 0.32 ± 0.06 / 158.8 ± 18.4 / 25.0 ± 3.3 / 14.0 ± 1.5
A2.Apc+/+ / 29 / 27.66 ± 0.45 / 0.55 ± 0.07 / 0.25 ± 0.003 / 0.28 ± 0.07 / 101.5 ± 3.3 / 9.8 ± 3.2 / 0.0 ± 0.0
A2.ApcMin/+ / 29 / 26.14 ± 0.47 / 0.46 ± 0.04 / 0.25 ± 0.003 / 0.31 ± 0.09 / 110.6 ± 6.5 / 13.2 ± 3.1 / 6.6 ± 1.1
A7.Apc+/+ / 14 / 27.01 ± 0.33 / 0.42 ± 0.02 / 0.25 ± 0.003 / 0.24 ± 0.07 / 98.5 ± 7.8 / 16.1 ± 7.3 / 0.0 ± 0.0
A7.ApcMin/+ / 14 / 26.27 ± 0.36 / 0.42 ± 0.03 / 0.25 ± 0.004 / 0.36 ± 0.09 / 145.2 ± 13.0 / 28.5 ± 4.1 / 21.6 ± 2.3
A9.Apc+/+ / 5 / 25.85 ± 0.36 / 0.40 ± 0.04 / 0.24 ± 0.007 / 0.11 ± 0.09 / 119.3 ± 16.0 / 6.0 ± 1.7 / 0.0 ± 0.0
A9.ApcMin/+ / 5 / 24.80 ± 0.42 / 0.24 ± 0.02 / 0.25 ± 0.004 / 0.25 ± 0.003 / 155.0 ± 12.8 / 19.2 ± 1.2 / 27.4 ± 1.2
A17.Apc+/+ / 20 / 25.65 ± 0.34 / 0.34 ± 0.03 / 0.23 ± 0.003 / 0.24 ± 0.05 / 148.0 ± 16.4 / 19.9 ± 5.6 / 0.0 ± 0.0
A17.ApcMin/+ / 28 / 25.57 ± 0.31 / 0.27 ± 0.02 / 0.24 ± 0.002 / 0.25 ± 0.02 / 164.2 ± 16.0 / 24.0 ± 2.4 / 19.1 ± 1.6
B6.Apc+/+ / 46 / 31.32 ± 0.70*** / 1.29 ± 0.11*** / 0.28 ± 0.004** / 1.44 ± 0.21** / 237.1 ± 21.1*** / 125.0 ± 16.5** / 0.0 ± 0.0
B6.ApcMin/+ / 51 / 23.22 ± 0.70 / 0.36 ± 0.05 / 0.24 ± 0.006 / 0.61 ± 0.14 / 245.5 ± 9.8** / 47.3 ± 7.5* / 91.8 ± 4.8***
A2.Apc+/+ / 30 / 32.40 ± 0.81*** / 1.63 ± 0.17*** / 0.29 ± 0.006** / 2.02 ± 0.47** / 252.2 ± 7.8** / 159.3 ± 3.7*** / 0.0 ± 0.0
A2.ApcMin/+ / 34 / 29.63 ± 0.87*** / 1.07 ± 0.12*** / 0.28 ± 0.006** / 1.42 ± 0.23** / 226.1 ± 19.4*** / 146.5 ± 27.0** / 28.1 ± 2.6***
A7.Apc+/+ / 16 / 27.50 ± 0.40 / 0.61 ± 0.07 / 0.26 ± 0.005 / 0.47 ± 0.20 / 111.7 ± 9.9 / 31.0 ± 11.5 / 0.0 ± 0.0
A7.ApcMin/+ / 18 / 26.11 ± 0.50 / 0.35 ± 0.07 / 0.26 ± 0.005 / 0.22 ± 0.04 / 168.0 ± 5.9 / 18.0 ± 3.6 / 97.9 ± 13.0***
A9.Apc+/+ / 5 / 35.52 ± 0.45*** / 1.90 ± 0.01*** / 0.31 ± 0.005** / 0.92 ± 0.10** / 238.0 ± 16.9*** / 95.1 ± 15.4*** / 0.0 ± 0.0
A9.ApcMin/+ / 5 / 26.90 ± 0.76 / 0.50 ± 0.08** / 0.28 ± 0.002** / 0.74 ± 0.15* / 197.0 ± 6.0 / 63.0 ± 14.3* / 105.7 ± 4.7***
A17.Apc+/+ / 24 / 27.92 ± 0.54 / 0.67 ± 0.09 / 0.26 ± 0.004 / 0.35 ± 0.10 / 137.4 ± 13.5 / 18.9 ± 6.3 / 0.0 ± 0.0
A17.ApcMin/+ / 24 / 25.63 ± 0.36 / 0.45 ± 0.04 / 0.25 ± 0.003 / 0.54 ± 0.07 / 158.7 ± 6.5 / 45.1 ± 7.6 / 62.4 ± 4.4***
Strain / N / Fat Source / Diet Duration / FBW (g) ± SEM / EFPM (g) ± SEM / BMI ± SEM / Fasting Insulin (µg/L) ± SEM / Fasting Glucose (mg/dL) ± SEM / Total Polyp Number
B6.Apc+/+ / 8 / Coconut / 30 / 23.89 ± 0.80 / 0.35 ± 0.03 / 0.24 ± 0.007 / 0.36 ± 0.01 / 132.7 ± 2.9 / 0.0 ± 0.0
B6.ApcMin/+ / 9 / Coconut / 30 / 22.78 ± 0.68 / 0.30 ± 0.03 / 0.24 ± 0.006 / 0.53 ± 0.09 / 147.3 ± 17.2 / 11.7 ± 1.4
B6.Apc+/+ / 7 / Coconut / 30 / 25.27 ± 0.75 / 0.54 ± 0.06 / 0.24 ± 0.005 / 0.35 ± 0.10 / 185.8 ± 4.0 / 0.0 ± 0.0
B6.ApcMin/+ / 10 / Coconut / 30 / 23.10 ± 0.29 / 0.39 ± 0.05 / 0.24 ± 0.005 / 0.67 ± 0.06 / 215.8 ± 11.6 / 81.6 ± 8.3***
B6.Apc+/+ / 10 / Corn / 30 / 23.25 ± 0.46 / 0.37 ± 0.03 / 0.22 ± 0.003 / 0.48 ± 0.05 / 149.0 ± 4.7 / 0.0 ± 0.0
B6.ApcMin/+ / 15 / Corn / 30 / 22.26 ± 0.28 / 0.42 ± 0.02 / 0.21 ± 0.003 / 0.24 ± 0.03 / 164.8 ± 6.9 / 27.2 ± 1.8
B6.Apc+/+ / 8 / Corn / 30 / 22.50 ± 0.82 / 0.39 ± 0.06 / 0.22 ± 0.005 / 0.32 ± 0.08 / 194.5 ± 6.7 / 0.0 ± 0.0
B6.ApcMin/+ / 10 / Corn / 30 / 22.64 ± 0.47 / 0.34 ± 0.03 / 0.23 ± 0.005 / 0.42 ±0.08 / 164.0 ± 15.0 / 88.1 ± 5.7***
B6.Apc+/+ / 7 / Olive / 30 / 24.87 ± 1.09 / 0.39 ± 0.09 / 0.25 ± 0.009 / 0.27 ± 0.05 / 108.4 ± 8.4 / 0.0 ± 0.0
B6.ApcMin/+ / 7 / Olive / 30 / 23.58 ± 0.51 / 0.34 ± 0.04 / 0.25 ± 0.007 / 0.26 ± 0.12 / 150.0 ± 18.7 / 15.9 ± 2.6
B6.Apc+/+ / 6 / Olive / 30 / 25.49 ± 1.05 / 0.44 ± 0.11 / 0.26 ± 0.008 / 0.30 ± 0.06 / 150.3 ± 10.1 / 0.0 ± 0.0
B6.ApcMin/+ / 8 / Olive / 30 / 24.14 ± 0.78 / 0.43 ± 0.06 / 0.25 ± 0.007 / 0.29 ± 0.02 / 153.0 ± 12.2 / 24.8 ± 1.5###
B6.Apc+/+ / 54 / Coconut / 60 / 27.91 ± 0.24 / 0.61 ± 0.12 / 0.24 ± 0.008 / 0.34 ± 0.14 / 92.4 ± 4.2 / 0.0 ± 0.0
B6.ApcMin/+ / 54 / Coconut / 60 / 25.78 ± 0.24 / 0.32 ± 0.04 / 0.25 ± 0.002 / 0.32 ± 0.06 / 158.8 ± 18.4 / 14.0 ± 1.5
B6.Apc+/+ / 46 / Coconut / 60 / 31.32 ± 0.70*** / 1.29 ± 0.11*** / 0.28 ± 0.004** / 1.44 ± 0.21** / 237.1 ± 21.1*** / 0.0 ± 0.0
B6.ApcMin/+ / 51 / Coconut / 60 / 23.22 ± 0.70 / 0.36 ± 0.05 / 0.24 ± 0.006 / 0.61 ± 0.14 / 245.5 ± 9.8** / 91.8 ± 4.8***
B6.Apc+/+ / 12 / Corn / 60 / 26.91 ± 0.28 / 0.38 ± 0.02 / 0.26 ± 0.003 / 0.22 ± 0.08 / 92.3 ± 5.0 / 0.0 ± 0.0
B6.ApcMin/+ / 7 / Corn / 60 / 26.42 ± 0.85 / 0.36 ± 0.04 / 0.27 ± 0.003 / 0.21 ± 0.41 / 153.4 ± 3.6 / 27.5 ± 1.5
B6.Apc+/+ / 13 / Corn / 60 / 26.28 ± 0.78 / 0.60 ± 0.08 / 0.24 ± 0.008 / 0.43 ± 0.09** / 196.2 ± 8.3*** / 0.0 ± 0.0
B6.ApcMin/+ / 14 / Corn / 60 / 21.72 ± 0.57 / 0.22 ± 0.03 / 0.22 ± 0.005 / 0.55 ± 0.17** / 201.2 ± 10.5** / 102.6 ± 8.0***
B6.Apc+/+ / 7 / Olive / 60 / 26.70 ± 0.46 / 0.41 ± 0.03 / 0.26 ± 0.005 / 0.27 ± 0.02 / 89.2 ± 5.1 / 0.0 ± 0.0
B6.ApcMin/+ / 8 / Olive / 60 / 25.03 ± 0.46 / 0.40 ± 0.07 / 0.27 ± 0.006 / 0.36 ± 0.07 / 153.0 ± 3.6 / 24.2 ± 2.8
B6.Apc+/+ / 18 / Olive / 60 / 30.94 ± 1.06** / 1.14 ± 0.12*** / 0.30 ± 0.008*** / 0.23 ± 0.17 / 142.3 ± 7.8 / 0.0 ± 0.0
B6.ApcMin/+ / 14 / Olive / 60 / 26.54 ± 0.58 / 0.57 ± 0.07 / 0.27 ± 0.006 / 0.38 ± 0.10 / 136.5 ± 7.5 / 25.7 ± 1.2###

Supplementary Table 4. B6.Apc+/+ and B6.ApcMin/+ after 30 or 60 days on the coconut, corn or olive oil diets: Final body weight (FBW), epididymal fat pad mass (EFPM), body mass index (BMI), fasting insulin, fasting glucose, and total polyp number. Grey shaded areas denote mice fed the LFD. For statistical analysis, each strain fed the HFD was compared to the same strain fed the LFD. ** P<0.001, *** P<0.0001. ### P<0.0001 compared to B6.ApcMin/+ fed the HFDCoco diet.

Strain / N / Drug Treatment / FBW (g) ± SEM / EFPM (g) ± SEM / BMI ± SEM / Fasting Insulin (µg/L) ± SEM / Fasting Glucose (mg/dL) ± SEM / Total Polyp Number
B6.Apc+/+ / 8 / N/A / 23.89 ± 0.80 / 0.35 ± 0.03 / 0.24 ± 0.007 / 0.36 ± 0.01 / 132.7 ± 2.9 / 0.0 ± 0.0
B6.ApcMin/+ / 9 / N/A / 22.78 ± 0.68 / 0.30 ± 0.03 / 0.24 ± 0.006 / 0.53 ± 0.09 / 147.3 ± 17.2 / 11.7 ± 1.4
B6.Apc+/+ / 7 / N/A / 25.27 ± 0.75 / 0.54 ± 0.06 / 0.24 ± 0.005 / 0.35 ± 0.10 / 185.8 ± 4.0 / 0.0 ± 0.0
B6.ApcMin/+ / 10 / N/A / 23.10 ± 0.29 / 0.39 ± 0.05 / 0.24 ± 0.005 / 0.67 ± 0.06 / 215.8 ± 11.6 / 81.6 ± 8.3***
B6.C5aR+/-;Apc+/+ / 6 / N/A / 26.94 ± 1.50 / 0.28 ± 0.03 / 0.23 ± 0.01 / 0.34 ± 0.12 / 120.0 ± 4.3 / 0.0 ± 0.0
B6.C5aR-/-;Apc+/+ / 4 / N/A / 29.93 ± 2.03 / 0.28 ± 0.03 / 0.27 ± 0.01 / 0.15 ± 0.04 / 128.5 ± 7.4 / 0.0 ± 0.0
B6.C5aR+/-;ApcMin/+ / 5 / N/A / 25.54 ± 0.35 / 0.27 ± 0.009 / 0.25 ± 0.006 / 0.34 ± 0.06 / 155.8 ± 11.4 / 15.3 ± 2.4
B6.C5aR-/-;ApcMin/+ / 7 / N/A / 25.43 ± 0.47 / 0.26 ± 0.02 / 0.25 ± 0.006 / 0.35 ± 0.08 / 157.7 ± 8.0 / 22.6 ± 1.2
B6.C5aR+/-;Apc+/+ / 8 / N/A / 29.23 ± 1.38 / 0.91 ± 0.28 / 0.27 ± 0.01 / 0.18 ± 0.01 / 136.0 ± 9.9 / 0.0 ± 0.0
B6.C5aR-/-;Apc+/+ / 9 / N/A / 27.00 ± 0.89 / 0.54 ±0.07 / 0.26 ± 0.005 / 0.20 ± 0.04 / 117.3 ± 7.6 / 0.0 ± 0.0
B6.C5aR+/-;ApcMin/+ / 4 / N/A / 27.32 ± 1.23 / 0.73 ± 0.20 / 0.26 ± 0.003 / 0.63 ± 0.15 / 163.0 ± 7.4 / 33.3 ± 3.0###
B6.C5aR-/-;ApcMin/+ / 8 / N/A / 27.44 ± 0.76 / 0.53 ± 0.02 / 0.26 ± 0.004 / 0.47 ± 0.22 / 158.7 ± 15.4 / 22.4 ± 1.5###
B6.Apc+/+ / 5 / Control / 26.29 ± 0.24 / 0.31 ± 0.05 / 0.23 ± 0.008 / 0.33 ± 0.08 / 130.7 ± 11.7 / 0.0 ± 0.0
B6.ApcMin/+ / 6 / Control / 25.98 ± 0.39 / 0.29 ± 0.08 / 0.24 ± 0.005 / 0.49 ± 0.06 / 149.3 ± 15.3 / 18.6 ± 2.1
B6.Apc+/+ / 5 / PMX53 / 26.03 ± 0.82 / 0.45 ± 0.06 / 0.23 ± 0.006 / 0.23 ± 0.09 / 118.1 ± 8.2 / 0.0 ± 0.0
B6.ApcMin/+ / 5 / PMX53 / 25.91 ± 0.49 / 0.41 ± 0.05 / 0.23 ± 0.003 / 0.47 ± 0.11 / 132.6 ± 9.9 / 8.5 ± 1.6
B6.Apc+/+ / 13 / Control / 24.42 ± 0.60 / 0.55 ± 0.04 / 0.25 ± 0.004 / 0.22 ± 0.02 / 133.3 ± 7.5 / 0.0 ± 0.0
B6.ApcMin/+ / 13 / Control / 23.14 ± 0.56 / 0.50 ± 0.02 / 0.25 ± 0.006 / 0.42 ± 0.06 / 179.8 ± 8.6 / 77.5 ± 5.3
B6.Apc+/+ / 12 / PMX53 / 24.00 ± 0.70 / 0.54 ± 0.05 / 0.26 ± 0.004 / 0.22 ± 0.03 / 131.4 ± 8.01 / 0.0 ± 0.0
B6.ApcMin/+ / 18 / PMX53 / 23.59 ± 0.60 / 0.41 ± 0.02 / 0.25 ± 0.005 / 0.30 ± 0.03 / 152.2 ± 9.3 / 29.8 ± 1.9###

Supplementary Table 5. Complement C5aR-deficient and mice treated with the C5aR inhibitor after 30 days on the coconut diet: Final body weight (FBW), epididymal fat pad mass (EFPM), body mass index (BMI), fasting insulin, fasting glucose, and total polyp number. Grey shaded areas denote mice fed the LFD. For statistical analysis, each strain fed the HFDCoco was compared to the same strain fed the LFDCoco. ** P<0.001, *** P<0.0001 . ### P<0.0001 compared to B6.ApcMin/+ fed the HFDCoco diet.