Supplementary Material

Converging and Unique Mechanisms of Mechanotransduction at Adhesion Sites

Mitchell K.L. Han and Johan de Rooij

Dept. Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Stratenum 3.231, Universiteitsweg 100, 3584 CG, Utrecht, the Netherlands

Corresponding author: De Rooij, J. ()

Table S1. The Cadherin Adhesome

Proteins present in both BirAcadhesomes [1,2,3] / Proteins from the literature cadhesome [4] / Proteins confirmed by localization in [1]
ABLIM3 / MARK2 / ABI1 / CDH8 / LIMA1 / PVRL1 / ANXA1
ACBD3 / MARK3 / ABI2 / CDH9 / LIN7A / PVRL2 / ANXA2
AGFG1 / NAV2 / ABL1 / CGNL1 / LMO7 / RAC1 / BAIAP2
ATP2B4 / NHS / ACP1 / CLIP1 / LPP / RACGAP1 / BAIAP2L1
BSG / NHSL1 / ACTB / CSK / MAGI1 / RAP1A / BMPR2
CANT1 / NOTCH2 / ACTN1 / CSNK1E / MAPK8 / RAPGEF1 / CDC42EP1
CLINT1 / NUMBL / ACTN4 / CSNK2A1 / MET / RAPGEF2 / CDC42EP4
CRK / OCLN / ACTR3 / CTNNA1 / MLLT4 / RDX / CDCA3
DSC3 / PEAK1 / ADAM10 / CTNNA2 / MPP7 / RHOA / CNN3
DSG2 / PFN1 / ADAM9 / CTNNA3 / MSN / ROCK1 / CXADR
DST / PGRMC2 / ADD1 / CTNNB1 / MYH10 / SCRIB / FLOT1
EFR3A / PHACTR4 / AJAP1 / CTNND1 / MYH9 / SDC1 / FRS2
EHBP1L1 / PICALM / AKAP5 / CTNND2 / MYO6 / SDCBP / KRT18
EHD1 / PLEKHA1 / ANK3 / CTTN / MYO7A / SH3BP1 / PAK4
EPHA2 / PLEKHA5 / ARF6 / DBN1 / NF2 / SHC1 / PDLIM1
EPN2 / PLEKHA6 / ARHGAP12 / DIAPH1 / NME1 / SHROOM3 / PDLIM5
EPS15L1 / PLEKHO2 / ARHGAP21 / DLC1 / NUMB / SIPA1L3 / PDLIM7
ERC1 / PPP1R13B / ARHGAP32 / DLG1 / PAK1 / SORBS1 / PKP3
FAM110C / PPP1R13L / ARHGAP35 / DLG5 / PALLD / SORBS3 / PPP1CA
FAM83B / SKT / ARHGAP5 / DYNC1H1 / PARD3 / SPTBN1 / PVRL4
FAM84B / SLC30A1 / ARVCF / ECT2 / PDZD2 / SRC / RAB10
GGA3 / SLC3A2 / CAPN1 / EGFR / PIK3CA / SSX2IP / S100A11
GOLGA2 / SLC4A7 / CAPZA1 / ENAH / PIP2 / SVIL / SNAP23
GOLGA3 / SNAP29 / CASP3 / EPB41 / PIP5K1C / SYK / STXBP6
GOLGA5 / SYAP1 / CBLL1 / ERBB2IP / PKD1 / TIAM1 / TMOD3
GOLGB1 / TBC1D22B / CD151 / EXOC3 / PKP4 / TJP1 / TRIM29
GORASP2 / TMEM199 / CD2AP / EZR / PLEKHA7 / TJP2
HSPA8 / TMEM87A / CDC42 / FAT1 / PPP2CA / TLN1
KANK2 / TPD52L2 / CDH1 / FBLIM1 / PRKCD / TRIO
KIAA1217 / TRIP11 / CDH10 / FER / PRKD1 / TRIOBP
KIAA1462 / UACA / CDH11 / FLNB / PSEN1 / TRIP6
KIAA1522 / USP43 / CDH12 / FSCN1 / PTEN / TRPC4
KIDINS220 / USP6NL / CDH15 / FYN / PTK2B / TRPV4
LAD1 / USP8 / CDH18 / GNA12 / PTPN1 / TUBA1B
LDHA / WWOX / CDH19 / HEG1 / PTPN11 / VASP
LLGL1 / YWHAZ / CDH2 / ILK / PTPN12 / VCL
LSR / ZFYVE16 / CDH20 / IQGAP1 / PTPN14 / VEZT
LZTS2 / CDH22 / ITGA3 / PTPN6 / VIM
CDH24 / ITGB1 / PTPRF / WASF2
CDH3 / JUB / PTPRJ / WASL
CDH4 / JUP / PTPRK / WIPF2
CDH5 / KEAP1 / PTPRM / ZYX
CDH6 / KIAA1543 / PTPRT
CDH7 / KRIT1 / PTPRU

Table S2. The Consensus Integrin Adhesomea

Name Used in Fig. 2 / Shared? / Gene nameb / Protein name
Core adhesome
α-actinin1 / yes / ACTN1 / actinin, alpha 1
α-actinin4 / yes / ACTN4 / actinin, alpha 4
PIX / no / ARHGEF7 / Rho guanine nucleotide exchange factor (GEF) 7
caldesmon / no / CALD1 / caldesmon 1
calponin / yes / CNN2 / calponin 2
CSK / yes / CSK / c-src tyrosine kinase
migfilin / yes / FBLIM1 / filamin binding LIM protein 1
kindlin2 / yes / FERMT2 / fermitin family member 2
filamin / yes / FLNC / filamin C, gamma
GIT / no / GIT2 / G protein-coupled receptor kinase interacting ArfGAP 2
ILK / yes / ILK / integrin-linked kinase
IQGAP1 / yes / IQGAP1 / IQ motif containing GTPase activating protein 1
LASP1 / no / LASP1 / LIM and SH3 protein 1
pinch / no / LIMS1 / LIM and senescent cell antigen-like domains 1
LPP / yes / LPP / LIM domain containing preferred translocation partner in lipoma
palladin / yes / PALLD / palladin, cytoskeletal associated protein
parvin / no / PARVA / parvin, alpha
PDLIM1 / yes / PDLIM1 / PDZ and LIM domain 1
PDLIM5 / yes / PDLIM5 / PDZ and LIM domain 5
PDLIM7 / yes / PDLIM7 / PDZ and LIM domain 7 (enigma)
plastin / no / PLS3 / plastin 3
FAK (PYK2) / yes / PTK2 (PTK2b) / protein tyrosine kinase 2, focal adhesion kinase
PXN / no / PXN / paxillin
RSU1 / no / RSU1 / Ras suppressor protein 1
ponsin / yes / SORBS1 / sorbin and SH3 domain containing 1
vinexin / yes / SORBS3 / sorbin and SH3 domain containing 3
TES / yes / TES / testis derived transcript (3 LIM domains)
Hic5 / no / TGFB1I1 / transforming growth factor beta 1 induced transcript 1
TRIP6 / yes / TRIP6 / thyroid hormone receptor interactor 6
VASP / yes / VASP / vasodilator-stimulated phosphoprotein
vinculin / yes / VCL / vinculin
zyxin / yes / ZYX / zyxin
Integrin, or only integrin-subunit interaction
talin / yes / TLN1 / talin 1
ANXA1 / yes / ANXA1 / annexin A1
FHL2 / no / FHL2 / four and a half LIM domains 2
FHL3 / no / FHL3 / four and a half LIM domains 3
ITGA5 / no / ITGA5 / integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
ITGAV / no / ITGAV / integrin, alpha V
ITGB1 / yes / ITGB1 / integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2 ,MSK12)
ITGB3 / no / ITGB3 / integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
TNS3 / no / TNS3 / tensin 3
No known interaction to other adhesome protein or F-actin
TGM2 / no / TGM2 / transglutaminase 2
ALYREF / no / ALYREF / Aly/REF export factor
BRIX1 / no / BRIX1 / BRX1, biogenesis of ribosomes, homolog (S. cerevisiae)
DDX18 / no / DDX18 / DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
DDX27 / no / DDX27 / DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
DIMT1 / no / DIMT1 / DIM1 dimethyladenosinetransferase 1 homolog (S. cerevisiae)
Hsp40 / no / DNAJB1 / DnaJ (Hsp40) homolog, subfamily B, member 1
FAU / no / FAU / Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed
FEN1 / no / FEN1 / flap structure-specific endonuclease 1
H1FX / no / H1FX / H1 histone family, member X
HP1BP3 / no / HP1BP3 / heterochromatin protein 1, binding protein 3
LIMD1 / no / LIMD1 / LIM domains containing 1
MRTO4 / no / MRTO4 / mRNA turnover 4 homolog (S. cerevisiae)
P4HB / no / P4HB / prolyl 4-hydroxylase, beta polypeptide
POLDIP3 / no / POLDIP3 / polymerase (DNA-directed), delta interacting protein 3
PPIB / no / PPIB / peptidylprolyl isomerase B (cyclophilin B)
RPL23A / no / RPL23A / ribosomal protein L23a
SIPA1 / yes / SIPA1 / signal-induced proliferation-associated 1
SYNCRIP / no / SYNCRIP / synaptotagmin binding, cytoplasmic RNA interacting protein

aThis table is adapted from [5].

bThe Gene names were used to compare proteins between integrin and cadherin adhesomes.

Supplementary references

S1Guo, Z., et al. (2014) E-cadherin interactome complexity and robustness resolved by quantitative proteomics.Sci Signal 7, rs7

S2 Van Itallie, C.M., et al.(2014) Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci 127, 885-895

S3 Padmanabhan, A., et al.(2015) Jack of all trades: functional modularity in the adherens junction. CurrOpin Cell Biol 36, 32-40

S4 Zaidel-Bar, R. (2013) Cadherin adhesome at a glance.J Cell Sci 126, 373-378

S5 Horton, E.R., et al.(2015) Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly.Nat Cell Biol 17, 1577-1587