Supplementary Table S1. Oligos Used in This Study

Supplementary Table S1. Oligos used in this study.

Table S1a:Oligos used for cloning pri-miRNAs.

Oligo name / Sequence (5’3’) / Description
Pri-has-miR-27a-s
Pri-has-miR-27a-as / GCCAAGCTTTGCCACCGAGGATGCTG
GCCCTCGAGAGCAGGATGGCAGGCAGAC / For cloning human pri-miR-27a to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-27a-s
pri-mmu-miR-27a-as / GCCAAGCTTTCTGCCTTGGGGGCTCCTGTC
GCCCTCGAGAGATGGGGTCCAGGGGGCG / For cloning pri-mmu-miR-27a to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-146b-s
pri-mmu-miR-146b-as / GCCAAGCTTCACAAGGCACACACTGAATAAATG
GCCCTCGAGTGTTTGGAGCCTGAAGGTGGT / For cloning pri-mmu-miR-146b to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-20a-s
pri-mmu-miR-20a-as / GCCAAGCTTTGGCCTGCTATTTACTTCAAGTGT
GCCCTCGAGGCTCAATAACAGGACAGTTGGCT / For cloning pri-mmu-miR-20a to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-142-5p-s
pri-mmu-miR-142-5p-as / GCCAAGCTTGTGGCCTGAAGAATCCCCGT
GCCCTCGAGCGTGGCGTGGTCTCCGAA / For cloning pri-mmu-miR-142-5p to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-17-s
pri-mmu-miR-17-as / GCCAAGCTTGCAGCGCCCACTGTGGCT
GCCCTCGAGCCTCACCTTGGGACTGAAGCT / For cloning pri-mmu-miR-17 to pcDNA3.1 in HindIII and Xho I enzyme sites
Pri-mmu-miR-155-s
pri-mmu-miR-155-as / GCCAAGCTTGCCATTTGTTCCATGTGAGTGC
GCCCTCGAGCATATCCCTTATCCTCTGGCTGCT / For cloning pri-mmu-miR-155 to pcDNA3.1 in HindIII and Xho I enzyme sites

Table S1b: Oligos used for cloning 3′UTR regions.

Oligo name / Sequence (5’3’) / Description
P38 3’UTR s
P38 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGATATCGAGTGGAAGAGCCTGACCTATGAT GCTCTAGACAAAGATAGGTGGACAGACGAAC
TCTATCTCACTTCAAGAGTAGGGGAAGAC
AAGGTCTTCCCCTACTCTTGAAGTGAGAT / For cloning human p383’UTR and mutated p38 3’UTR to PIS2 vector in EcoR V and XbaI enzyme sites
MAP2K4 3’UTR s
MAP2K4 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGAGCTCATGTCTGTATACTCCTGTCAC
GCGATATCGAAGAAAGTAAATTCCTCCTTTG
ATCAAGTTGTTGAACACAATTAGATCA
GATGTGATCTAATTGTGTTCAACAACT / For cloning human MAP2K4 3’UTR and mutated MAP2K4 3’UTR to PIS2 vector in Sac I and EcoRV enzyme sites
MAP2K7 3’UTR s
MAP2K7 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGAGCTCGAAGACTGAGTCACCGCGGAC
GCGATATCCACGCTGGGAGCGTGCAT
CCCCCGACAGACAACACAAACGGAAGA GCTGTCTTCCGTTTGTGTTGTCTGTCG / For cloning human MAP2K7 3’UTR and mutated MAP2K7 3’UTR to PIS2 vector in Sac I and EcoRV enzyme sites
TAB3 3’UTR s
TAB3 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGATATC AAGAATGTACACACAGAAGTCAAGA
GCTCTAGA AGCAGCAATTACAAATACCCT
CAGTTTATGCAAAACACAAATTCCCAG GCTCTGGGAATTTGTGTTTTGCATAAA / For cloning human TAB3 3’UTR and mutated TAB3 3’UTR to PIS2 vector in EcoRV and XbaI enzyme sites
FADD 3’UTR s
FADD 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGATATCCCGATGTCATGGAACTCAGACG
GCTCTAGAAACGCCACAGTGGTTGAGCAT
AGGTAGCCCAGCAACACAAAGACCCAG CCTGCTGGGTCTTTGTGTTGCTGGGCT / For cloning human FADD 3’UTR and mutated FADD 3’UTR to PIS2 vector in EcoRV and XbaI enzyme sites
STAT 3’UTR s
STAT 3’UTR as
3’UTR mutant s
3’UTR mutant as / GCGAGCTCTGCAGAAAGATACGACTGAGG
GCGATATCGAGTATGTAGCTATAGGTGGCCTG
TGAGCAATCTGGGAGACCTTAAAAATA
TCTCTATTTTTAAGGTCTCCCAGATTG / For cloning human STAT 3’UTR and mutated STAT3 3’UTR to PIS2 vector in Sac I and XbaI enzyme sites

Table S1c: Oligos used for cloning promoter regions.

Oligo name / Sequence (5’3’) / Description
-1357fragment as
-1357 fragment s
-966 fragment s
-865 fragment s
-366 fragment s
-243 fragment s
-178 fragment s
-103 fragment s
-74 fragment s / GAAGATCTGGACAAGGAGGCTACAGCAACT (Bg1-II)
CCGCTCGAGACCACTCCTGGCTGATTTTTTAGTG(Xho I)
CCGCTCGAGGAGACCAGCCTGGCCAACATG (Xho I)
CTAGCTAGCAAAAATACAAAACTTAACTGGGCGTG(Nhe I)
CCGCTCGAGACTCTGGAGGTGGAGGTTGC (Xho I)
CCGCTCGAGGGGCACTTACTGAGTGACATACATACT (Xho I)
GGGGTACCTGAACCTTGGGCTGTTTGG (Kpn I)
CTAGCTAGCGGGGTGCGTGTCTAGAAAGAG (Nhe I)
CTAGCTAGCGGGAGGCCTGGTGGTTTCC (Nhe I) / For cloning mutant fragment of miR-27a promoter into pGL3 vector
SP1 binding region (-1066_-1058 mutation) / CAAAAATTAGGGCCAGACACGGTGGCTCACGCC
GGATTACAGGCGTGAGCCACCGTGTCTGGCCC / For cloning mutant fragment of miR-27a promoter into pGL3 vector with cloning fragment primers
SP1 binding region (-932_-923 mutation) / CAAAACTTAACTGGACACGGTGGC
CCCGCCACCGTGTCCAGTTAAG / For cloning mutant fragment of miR-27a promoter into pGL3 vector with cloning fragment primers
SP1 binding region (-749_-741 mutation) / GAAAAAATTAGCCGGGACACGTGGTGC
CAGCTATGCACCACGTGTCCCGGC / For cloning mutant fragment of miR-27a promoter into pGL3 vector with cloning fragment primers
SP1 binding region (-185_-177 mutation) / CCTGTGCTCTGGGGGGACACGGGTGCGTG
CTAGACACGCACCCGTGTCCCCCCAGAG / For cloning mutant fragment of miR-27a promoter into pGL3 vector with cloning fragment primers
SP1 binding region (-83_-75 mutation) / GACCGGGCCACGGGACACGAGGCCCTG
CACCAGGGCCTCGTGTCCCGTGGCCC / For cloning mutant fragment of miR-27a promoter into pGL3 vector with cloning fragment primers

Table S1d: Oligos used for cloning SP1.

Oligo name / Sequence (5’3’) / Description
SP1 s
SP1 as
SP1(deleted 620-785aa) s
SP1(deleted 620-785aa) as / ATGAGCGACCAAGATCACTCCATG
GAAGCCATTGCCACTGATATTAATGG
ATGAGCGACCAAGATCACTCCATG
AGGATCCCCCGAGCCCCTTCCTTC / For cloning human SP1 and mutated SP1 into pCDNA.3.1 Topo/His

Table S1e: Oligos used for PCR analyses.

Oligo name / Sequence (5’3’) / Description
Human P38 s
Human P38 as / ATGCGAAAAGAACCTACAGAGAACT
GGGTCACCAGATACACATCATTG / qRT-PCR for human p38 detection
Mouse P38 s
Mouse P38 as / ACCTACTGGAGAAGATGCTCGTTT
GACTTCATCATAGGTCAGGCTCTTC / qRT-PCR for mouse p38 detection
MAP2K4 s
MAP2K4 as / GCAAGGTTTACTCTGAATCCCAA
AACCATAAGCTCCTCGTCCAATT / qRT-PCR for human MAP2K4 detection
MAP2K7 s
MAP2K7 as / TGGCAACAGGACAGTTTCCC
CGCTTGATGAAGCTGTGTTCA / qRT-PCR for human MAP2K7 detection
FADD s
FADD as / GAGCGGCCTAGACCTATTCTC
CCAGGTGGGCCACTGTTGC / qRT-PCR for human FADD detection
IRAK2 s
IRAK2 as / GAGTGGGACTGGATGGAGTTCG
CCGGTTTCCAGTTCAGGATGA / qRT-PCR for human IRAK2 detection
IRAK4 s
IRAK4 as / ATAACTTTGATGAACGACCCATTTC
TCTCCATCACTTGAGAAACCAAGTA / qRT-PCR for human IRAK4 detection
TAB 1 s
TAB 1 as / ACCAGCAAGACCAGCGTGAC
TGCGTGTTGGTGGACTGC / qRT-PCR for human TAB1 detection
TAB 2 s
TAB2 as / CAGCCTGGTCCCTGGACTACT
ATGAATGGTTGGTGGTTGTGAA / qRT-PCR for human TAB2 detection
TAB3 s
TAB3 as / GCTGTTACATCAACGAGCAAGG
GCGTAGGGATCGCAGTGGT / qRT-PCR for human TAB3 detection
IRF4 s
IRF4 as / GACCCTCAGAGGCAAAGAAAGCT
TCTTGAATAGAGGAATGGCGGATAG / qRT-PCR for human IRF4 detection
IRF5 s
IRF5 as / CAGGTGGAACTCTTCGGCC
TTTGCGGTCAGGCCATTCTT / qRT-PCR for human IRF5 detection
IRF8 s
IRF8 as / CCGAGCCATACAAAGTTTACCG
CAGAGCGACCGCACTCCA / qRT-PCR for human IRF8 detection
MAP3K14 s
MAP3K14 as / TCTCGTGGTGCCCATGAGA
ATCCTGTGCACCTCTCCGAAG / qRT-PCR for human MAP3K14 detection
TNFα s
TNFα as / ATGAGCACTGAAAGCATGATCCGGGAC
TCACAGGGCAATGATCCCAAAGTAG / RT-PCR for human TNFαdetection
IFNα s
IFNα as / ATGGCCTCGCCCTTTGCTTTACTGATG
CCTTCCTCCTTAATCTTTCTTGCAAG / RT-PCR for human IFNαdetection
IL-1β s
IL-1β as / ATGGCAGAAGTACCTGAGCTCGCCAG
GGAAGACACAAATTGCATGGTG / RT-PCR for human IL-1β detection
IL-6 s
IL-6 as / ATGAACTCCTTCTCCACAAGCGCCTTC
CTACATTTGCCGAAGAGCCCTC / RT-PCR for human IL-6 detection
IL-12p40 s
IL-12p40 as / ATGTGTCACCAGCAGTTGGTCATCTC
CTAACTGCAGGGCACAGATGC / RT-PCR for human IL-12p40 detection
IL-23A s
IL-23A as / ATGCTGGGGAGCAGAGCTGTAATG
GGGACTCAGGGTTGCTGCTCC / RT-PCR for human IL-23 detection
IL-10 s
IL-10 as / ATGCACAGCTCAGCACTGCTCTGTTG
TCAGTTTCGTATCTTCATTGTCATG / RT-PCR for human IL-10 detection
SP1 s
SP1 as / TGGCTGCCGCTCCCAACTTA
ATGATGTTGCCTCCACTTCCTCG / qRT-PCR for human SP1 detection
AML-1 s
AML-1 as / CGAAGACATCGGCAGAAACTAGA
CTGAGGGTTAAAGGCAGTGGAGT / qRT-PCR for human AML-1 detection
IRF7 s
IRF7 as / ACCCTGGCTGTGCCGAGT
AAGCACTCGATGTCGTCATAGAG / RT-PCR for human IRF7 detection
SMAD2 s
SMAD2 as / CTTGATGGTCGTCTCCAGGTATC
TCGCAGTTTTCAATTGCCTTG / RT-PCR for human SMAD2 detection
SMAD3 s
SMAD3 as / TGCCTTTCACTCCCCCGAT
TGCCCCGTCTTCTTGAGTTTC / RT-PCR for human SMAD3 detection
SMAD4 s
SMAD4 as / CCCAGGATCAGTAGGTGGAATAG
AGGTTGTGGGTCTGCAATCG / RT-PCR for human SMAD4 detection
SMAD6 s
SMAD6 as / CTGTCCGATTCCACATTGTCTTA
TGGTCGTACACCGCATAGAGG / RT-PCR for human SMAD6 detection
SMAD7 s
SMAD7 as / ACACTGGTGCGTGGTGGCATAC
AAAGCCATTCCCCTGAGGTAGAT / RT-PCR for human SMAD7 detection
CBP s
CBP as / CAGCATTGATAACAGAGTCCCTACC
TTGGATTCACCAGGATCGGG / qRT-PCR for human CBP detection
Has-pre-miR-27a s
Has-pre-miR-27a as / AAATCTCATTACCTCCTTTGCTCTC
AACCAACTGTGTTTCAGCTCAGTAG / qRT-PCR for has-pri-miR-27a detection
mmu-pre-miR-27a s
mmu-pre-miR-27a as / GATTTCCAACTGACCCTGTGCT
ACTGGAATCAACTGTTTCAGCTCA / qRT-PCR for mmu-pri-miR-27a detection
Mouse GAPDH s
Mouse GAPDH as / TCAACGGCACAGTCAAGG
TACTCAGCACCGGCCTCA / qRT-PCR for mouse GAPDH detection
Human GAPDH s
Human GAPDH as / GTTGCCATCAATGACCCCTTC
CTTCTCCATGGTGGTGAAGAC / qRT-PCR for human GAPDH detection

1