Resume
Hailin Wang, Ph D
Environmental Health Sciences,
Department of Public Health Sciences,
University of Alberta
10-110 Clinical Sciences building,
Edmonton, Alberta, Canada T6G 2G3
Email:
TEL: 1-780-492-7776
FAX: 1-780-492-7800
EDUCATION
PhD 09/1994-06/1997. Chinese Academy of Sciences, Dalian Institute of Chemical Physics. Major in Analytical Chemistry.
MSc 09/1991-08/1994. Chinese Academy of Sciences, Dalian Institute of Chemical Physics. Major in Analytical Chemistry.
BSc 09/1987-08/1991. Department of Chemistry, Wuhan University, Major in Analytical Chemistry.
Work EXPERIENCE:
Postdoctoral Fellow/Research Associate, 07/2000-present. Environmental Health Sciences Program, Department of Public Health Sciences, University of Alberta
Associate Professor, 04/1999-07/2000. Chinese Academy of Sciences, Dalian Institute of Chemical Physics.
Assistant Professor, 07/1997-04/1999. Chinese Academy of Sciences, Dalian Institute of Chemical Physics.
AWARDS:
A. PRESIDENTIAL SPECIAL AWARD for graduate student by Chinese Academy
of Sciences. (10/1997).
B. Excellent Student Prize Granted by Chinese Academy of Sciences, Dalian Institute of Chemical Physics. (07/1997).
C. CAIA (Chinese Association of Instrumental Analysis) awards for three years (1995, 1998, and 1999).
PAST RESEARCH PROJECTS:
· Novel chromatographic techniques for isolation and purification of proteins and enzymes (membrane chromatography and monolithic chromatography).
· Studies of interactions of drug-protein and metal ion-metalloprotein using microdialysis/HPLC.
· Protein analysis, peptide mapping and fingerprinting analysis using HPLC, CE, and MADLI-TOF MS.
· Enzyme microreactors.
· Immobilization of proteins, enzymes, DNA and related binding partners on artificial membranes and silicon to develop novel biochromatographic technique.
· Screening and analysis of biologically active components in natural products and Chinese medicine using affinity chromatography, microdialysis/HPLC, and MADLI-TOF MS.
CURRENT RESEARCH INTEREST
· Affinity capillary electrophoresis. Investigating DNA-Protein interaction and antigen-antibody interaction to clarify the binding stoichiometry and the underlined mechanisms.
· DNA damage and repair. Developing novel ultrasensitive methods and techniques for detection of DNA damage. Techniques include capillary electrophoresis and laser-induced fluorescence, and single cell gel electrophoresis (comet assay).
· Assessment of human exposure to carcinogens using DNA adducts as biomarkers. Assessment of genotoxicity of environmental carcinogens, such as benzo(a)pyrene and arsenic, present at the levels relevant to environmental exposure.
SELECTIVE PUBLICATIONS:
1. Le, X.C.; Pavski, V.; Wang, H. Affinity recognition, capillary electrophoresis and laser induced fluorescence polarization for ultrasensitive bioanalysis. Submitted to Canadian Journal of Chemistry. (Invited paper).
2. Wang, H.; Lu, M.; Mei, N.; Lee, J.; Weinfeld, M.; Le, X. C. Immunoassay using capillary electrophoresis laser induced fluorescence detection for DNA adducts. Analytica Chimica Acta, 500, 13-20 (2003). (Invited paper).
3. Wang, H.; Lu, M.; Weinfeld, M.; Le, X.C. Enhancement of immunocomplex detection and application to assays for DNA adduct of benzo[a]pyrene. Analytical Chemistry, 75, 247-254 (2003).
4. Wang, H.; Xing, J.; Tan, W.; Lam, M.; Carnelley, T.; Weinfeld, M.; Le, X.C. Binding stoichiometry of DNA adducts with antibody studied by capillary electrophoresis and laser-induced fluorescence. Analytical Chemistry, 74, 3714-3719 (2002).
5. Carnelley, T.J.; Barker, S.; Wang, H.; Tan, W.G.; Weinfeld, M.; Le, X.C. Synthesis, characterization, and applications of a fluorescent DNA damage probe. Chemical Research in Toxicology, 14, 1513-1522 (2001).
6. Tan, W.G.; Carnelley, T.J.; Murphy, P.; Wang, H.; Lee, J.; Barker, S.; Weinfeld, M.; Le, X.C. Detection of DNA adducts of benzo[a]pyrene using immuno-electrophoresis with laser-induced fluorescence: analysis of A549 cells. Journal of Chromatography A, 924, 377-386 (2001).
7. Guo, M.; Zou H.; Wang, H. Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography. Analytica Chimica Acta 443(1):91-99 (2001).
8. Luo, Q.; Zou, H.; Wang, H. Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate- co-ethylene dimethacrylate) for affinity chromatography. Chinese J. Anal. Chem. 29(5):497-501 (2001).
9. Jiang, H.; Zou, H.; Wang, H.; Ni J.; Zhang, Y. On-line characterization of the activity and reaction kinetics of immobilized enzyme by high performance frontal analysis. J. Chromatogr. A, 903(1-2):77-84 (2000).
10. Jiang, H.; Zou, H.; Wang, H.; Zhang, Q.; Ni, J.; Zhang, Q.-C.; Guo, Z.; Chen, X. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping (I). Science in China (Series B), 30(5):385-391 (2000).
11. Jiang, H.; Zou, H.; Wang, H.; Zhang, Q.; Ni, J.; Zhang, Q.-C.; Guo, Z.; Chen, X. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping (II). Science in China (Series B), 43(6):625-633 (2000).
12. Zhang, Q.; Zou, H.; Wang, H.; Ni, J. Synthesis of a silica-bonded BSA s-triazine chiral stationary for high-performance liquid chromatographic resolution of enantiomers. J. Chromatogr. A, 866:173~181 (2000).
13. Wang, H.; Kong, L.; Zou, H.; Ni, J.; Zhang, Y. Screening and analysis of biologically active compounds in angelica sinensis by molecular biochromatography. Chromatographia, 50:439 (1999).
14. Wang, H.; Zou, H.; Zhang, Y. Determination of ginsengoside Rg3 in plasma by solid phase extraction and high performance liquid chromatography for pharmacokinetic study. J. Chromatogr. B, 731:403 (1999).
15. Zou, H.; Wang, H.; Zhang, Y. Drug-protein interaction studied by technique of microdialysis combined with high performance liquid chromatography. Rewiews in Anal. Chem., 18:383 (1999) (Invited review).
16. Wang, H.; Zou, H.; Zhang, Y. Quantitative study of competitive binding of drugs to protein by microdialysis / high-performance liquid chromatography. Analytical Chemistry, 70(2):373-377 (1998). Reprint: HTML/PDF
18. Wang, H.; Zou; H.; Feng; A.; Zhang; Y. Binding of sulfamethoxazole to human serum albumin by a combined technique of microdialysis with high performance liquid chromatography. Analytica Chimica Acta, 342:159-165 (1997).
19. Wang, H.; Feng A.; Zou H.; Lee R.; Zhang J.; Chao R.; Zhang Y. Determination of bile Acids in human serum by high performance liquid chromatography with fluorescence Detection. Chinese J. Instr. Analysis, 16(5):18 (1997).
20. Wang, H.; Li, T.; Zou, H.; Zhang, Y.; Chao, J.; Chao, L. The purification of human urinary kallikrein with ion-exchange radial flow membrane chromatography. Biomed. Chromatogr., 10: 139 (1996).
21. Zou, H.; Wang, H.; Lee, R.; Zhang, Y. Separation mechanism of micellar-electrokinetic capillary chromatography studied by quantitive-structure retentionship. Progress in Nature Science (Chinese), 6: 683 (1996).
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