Supplementary Table S1: Locality collected, collector, date and methods used in this study

Sample Code / Collection Information / Collector/ Provider / Collection Date / Collection Method
der30 / 34.7197N 120.0415W / California: Santa Barbara Co. / M.C. / 2005.05.29-12 / Unknown
der31 / 34.6993N 118.5465W / California: Los Angeles Co. / M.C. / 2007.04.14-28 / Unknown
der32 / 34.4855N 120.1423W / California: Santa Barbara Co. / M.C. / 2003.03.05-19 / Malaise
der33 / APHIS Interception / Origin: India / J.F. / 2009.07.29 / Unknown
der38 / APHIS Interception / Origin: Pakistan / J.F. / Unknown / Unknown
der39 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der40 / APHIS Interception / Origin: Saudi Arabia / J.F. / 2010.05.06 / Unknown
der41 / APHIS Interception / Origin: Saudi Arabia / J.F. / 2010.11.03 / Unknown
der42 / APHIS Interception / Origin: Argentina? / J.F. / 2010.11.19 / Unknown
der43 / APHIS Interception / Origin: India / J.F. / 2010.12.20 / Unknown
der44 / APHIS Interception / Origin: Saudi Arabia / J.F. / 2011.01.13 / Unknown
der47 / APHIS Interception / Origin: Unknown / J.F. / 1993.03.01 / Unknown
der48 / APHIS Interception / Origin: Unknown / J.F. / 1993.03.01 / Unknown
der55 / APHIS Interception / Origin: Syria / J.F. / Unknown / Unknown
der63 / APHIS Interception / Origin: Syria / J.F. / Unknown / Unknown
der64 / APHIS Interception / Origin: Syria / J.F. / Unknown / Unknown
der68 / APHIS Interception / Origin: Australia / J.F. / Unknown / Unknown
der71 / APHIS Interception / Origin: Colorado Springs / J.F. / Unknown / Unknown
der72 / APHIS Interception / Origin: Unknown / J.F. / Unknown / Unknown
der80 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der83 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der84 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der85 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der86 / APHIS Interception / Origin: Pakistan / J.F. / Unknown / Unknown
der88 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der89 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der91 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der94 / APHIS Interception / Origin: Unknown / J.F. / Unknown / Unknown
der98 / APHIS Interception / Origin: Washington D.C. / J.F. / Unknown / Unknown
der104 / APHIS Interception / Michigan: Williamston / R.L.O.O. / 2012.04.29 / Unknown
der105 / 39.55.420N 122.11.489W / California: Corning / R.L.O.O. / 2012.05.18 / Lure
der106 / Unknown / Florida: Baldwin Co. / EDRR / 2012.04.03 / Unknown
der116 / 35.20.583N 120.25.574W / California: San Luis Obispo Co. / R.L.O.O. / 2012.05.17 / Lure
der118 / 39.24.265N 122.09.478W / California: Sacramento Co / R.L.O.O. / 2012.05.18 / Lure
der131 / 35.11.410N 119.54.791W / California: San Luis Obispo Co. / R.L.O.O. / 2012.05.14 / Lure
der132 / 35.22.297N 120.27.724W / California: San Luis Obispo Co. / R.L.O.O. / 2012.05.12 / Lure
der138 / 35.21.384N 120.28.271W / California: San Luis Obispo Co. / R.L.O.O. / 2012.05.14 / Lure
der175 / Unknown / Florida: Palmetto Co. / EDRR / 2012.05.22 / Unknown
der176 / 46.55.19N 96.46.17W / North Dakota: Fargo / R.L.O.O. / 2012.06.26 / Lure
der177 / 46.52.45N 94.55.00W / North Dakota: Fargo / R.L.O.O. / 2012.06.26-27 / Lure
der180 / 42.07.02N 84.41.42W / Michigan: Lansing / G.L.P. / 2012.06.02-08 / Lure
der184 / Unknown / Arizona: Yavapai Co. / Unknown / 1999.09.06 / Unknown
der193 / Unknown / Florida: Merritt Island / EDRR / 2012.03.16 / Unknown
der194 / Unknown / Florida: Baldwin Co. / EDRR / 2012.04.23 / Unknown
der196 / Unknown / Florida: Baldwin Co. / EDRR / 2012.04.23 / Unknown
der199 / Unknown / Florida: Apopka Co. / EDRR / 2012.03.27 / Unknown
der200 / Unknown / Florida: Apopka Co. / EDRR / 2012.03.27 / Unknown
der201 / Unknown / Florida: Baldwin Co. / EDRR / 2012.04.23 / Unknown
der202 / Unknown / Ohio: Youngstown Co. / EDRR / 2012.05.29 / Unknown
der211 / Unknown / Colorado: Boulder / APHIS / 2011.07.15 / Unknown
der216 / APHIS Interception / Origin: Panama / J.F. / Unknown / Unknown
der217 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der219 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der220 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der221 / APHIS Interception / Origin: Pakistan / J.F. / Unknown / Unknown
der222 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der223 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der224 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der225 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der226 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der228 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der229 / APHIS Interception / Origin: Saudi Arabia / J.F. / Unknown / Unknown
der230 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der231 / APHIS Interception / Origin: United Arab Emirates / J.F. / Unknown / Unknown
der232 / APHIS Interception / Origin: India / J.F. / Unknown / Unknown
der233 / APHIS Interception / Origin: Unknown / J.F. / Unknown / Unknown
der234 / APHIS Interception / Origin: United Arab Emirates / J.F. / Unknown / Unknown
der236 / APHIS Interception / Origin: United Arab Emirates / J.F. / Unknown / Unknown
der239 / APHIS Interception / Origin: Minnesota / J.F. / Unknown / Unknown
der241 / Unknown / Florida: Miami / EDRR / 2012.09.02 / Unknown
der243 / Unknown / Arizona: Flagstaff / S.B. / 2012.07.06 / Lure
der244 / Unknown / Pennsylvania / EDRR / Unknown / Unknown
der247 / Unknown / Michigan: East Lansing / A.I.C. / 2012.09.15-17 / Lure
der248 / Unknown / Florida: Dade Co. / W.E.W. / 1983.07.04 / Unknown
der249 / Unknown / Arizona: Dewey Co. / R.S.B. / 2000.04.06 / Unknown
der250 / Unknown / New Hampshire / J.M. / 2006 / Unknown
der251 / Unknown / Arizona: Yavapai Co. / R.S.B. / 2012.06.18 / Unknown
der252 / Unknown / Arizona: Yavapai Co. / R.S.B. / 1987.06.10 / Unknown
der253 / Unknown / Montana: Prairie Co. / Unknown / 1970.08 / Unknown
der254 / Unknown / New York / A.I.C. / Unknown / Lure
der259 / 35.42.27N 85.44.40W / Tennessee: NRC / A.B. / 2012.08.20-28 / Lure
der262 / Unknown / Colorado: Larimer Co. / APHIS / 2011.07.07 / Unknown
der270 / Unknown / Arizona: Yavapai Co. / R.S.B. / 2008.07.12 / Unknown
der271 / Unknown / Colorado: Weld Co. / APHIS / 2011.07.07 / Unknown
der277 / Unknown / Arizona: Flagstaff / S.B. / 2012.07.06 / Unknown
der278 / Unknown / Arizona: Flagstaff / S.B. / 2012.07.06 / Lure
der279 / 46.55.19N 96.46.17W / North Dakota: Fargo / R.L.O.O. / 2012.06.26 / Lure
der280 / 42.07.02N 84.31.42W / Michigan: Lansing / G.L.P. / 2012.06.11-17 / Lure

Note: Collector/provider initials refer to: Michael Caterino (M.C.), Joel Floyd, USDA APHIS (J.F.), Rachel Olson (RLOO), 2012 USDA APHIS Early Detection Rapid Response Survey targeting bark beetles (EDRR), Stephen Burr (S.B.), Anthony Cognato (A.I.C.), W.E. Wyles (W.E.W.), R.S. Beal, Jr. (R.S.B.), Alicia Bray (A.B.) and Gary L. Parsons (G.L.P.)

Supplementary Table S2. Identification of individuals, identifier, indication of use in real-time PCR, PCR well number corresponds to Figure S5, 16S haplotype corresponds to Tables S3 and S4, location of voucher specimen storage and GenBank Accession numbers.

Sample Code / Species Identification / Identifier / Real Time-PCR ID / Haplotypes / Stored at / GenBank Accession
number
der30 / T. sternale / M.C. / X / 1 / SBNHM / KJ930428
der31 / Megatoma spp / M.C. / X / 1 / SBNHM / KJ930429
der32 / Megatoma spp / M.C. / X / 2 / SBNHM / KJ930430
der33 / T. granarium / USDA / X / 2 / USDA / KJ930431
der38 / T. granarium / USDA / X / 3 / USDA / KJ930432
der39 / T. granarium / USDA / X / 1 / USDA / KJ930433
der40 / T. granarium / USDA / X / 1 / USDA / KJ930434
der41 / T. granarium / USDA / X / 1 / USDA / KJ930435
der42 / T. granarium / USDA / X / 2 / USDA / KJ930436
der43 / T. granarium / USDA / X / 2 / USDA / KJ930437
der44 / T. granarium / USDA / X / 3 / USDA / KJ930438
der47 / T. granarium / USDA / X / 3 / USDA / KJ930439
der48 / T. granarium / USDA / X / 2 / USDA / KJ930440
der55 / T. granarium / USDA / X / 1 / USDA / KJ930441
der63 / T. granarium / USDA / X / 3 / USDA / KJ930442
der64 / T. granarium / USDA / X / 1 / USDA / KJ930443
der68 / T. variable / USDA / X / 1 / USDA / KJ930444
der71 / T. simplex / USDA / X / 3 / USDA / KJ930445
der72 / T. variable / USDA / X / 1 / USDA / KJ930446
der80 / T. granarium / USDA / X / 3 / USDA / KJ930447
der83 / T. granarium / USDA / X / 3 / USDA / KJ930448
der84 / T. granarium / USDA / X / 2 / USDA / KJ930449
der85 / T. granarium / USDA / X / 2 / USDA / KJ930450
der86 / T. granarium / USDA / X / 3 / USDA / KJ930451
der88 / T. granarium / USDA / X / 3 / USDA / KJ930452
der89 / T. granarium / USDA / X / 2 / USDA / KJ930453
der91 / T. granarium / USDA / X / 2 / USDA / KJ930454
der94 / T. inclusum / USDA / X / 1 / USDA / KJ930455
der98 / T. simplex / USDA / X / 1 / USDA / KJ930456
der104 / T. inclusum / R.L.O.O. / X / 1 / MSU / KJ930457
der105 / T. glabrum / R.L.O.O. / X / 1 / MSU / KJ930458
der106 / T. glabrum / R.L.O.O. / X / 1 / MSU / KJ930459
der116 / T. sternale sternale / R.L.O.O. / X / 2 / MSU / KJ930460
der118 / T. simplex / R.L.O.O. / X / 3 / MSU / KJ930461
der131 / T. grassmani / R.L.O.O. / X / 1 / MSU / KJ930462
der132 / T. simplex / R.L.O.O. / X / 3 / MSU / KJ930463
der138 / Megatoma spp / R.L.O.O. / X / 2 / MSU / KJ930464
der175 / T. sternale sp / R.L.O.O. / X / 3 / MSU / KJ930465
der176 / T. variable / R.L.O.O. / X / 1 / MSU / KJ930466
der177 / T. teukton / R.L.O.O. / X / 1 / MSU / KJ930467
der180 / T. inclusum / R.L.O.O. / X / 1 / MSU / KJ930468
der184 / T. grassmani / R.S.B. / X / 2 / CPGAM / KJ930469
der193 / T. fasciferum / R.L.O.O. / X / 1 / MSU / KJ930470
der194 / T. variable / R.L.O.O. / X / 1 / MSU / KJ930471
der196 / T. ornatum / R.L.O.O. / X / 1 / MSU / KJ930472
der199 / T. fasciferum / R.L.O.O. / X / 2 / MSU / KJ930473
der200 / T. fasciferum / R.L.O.O. / X / 3 / MSU / KJ930474
der201 / T. sternale sternale / R.L.O.O. / X / 4 / MSU / KJ930475
der202 / T. ornatum / R.L.O.O. / X / 2 / MSU / KJ930476
der211 / T. simplex / R.L.O.O. / X / 3 / MSU / KJ930477
der216 / T. granarium / USDA / X / 2 / USDA / KJ930478
der217 / T. granarium / USDA / X / 2 / USDA / KJ930479
der219 / T. granarium / USDA / X / 1 / USDA / KJ930480
der220 / T. granarium / USDA / X / 1 / USDA / KJ930481
der221 / T. granarium / USDA / X / 3 / USDA / KJ930482
der222 / T. granarium / USDA / X / 3 / USDA / KJ930483
der223 / T. granarium / USDA / X / 3 / USDA / KJ930484
der224 / T. granarium / USDA / X / 1 / USDA / KJ930485
der225 / T. granarium / USDA / X / 1 / USDA / KJ930486
der226 / T. granarium / USDA / X / 3 / USDA / KJ930487
der228 / T. granarium / USDA / X / 1 / USDA / KJ930488
der229 / T. granarium / USDA / X / 2 / USDA / KJ930489
der230 / T. granarium / USDA / X / 3 / USDA / KJ930490
der231 / T. granarium / USDA / X / 3 / USDA / KJ930491
der232 / T. granarium / USDA / X / 1 / USDA / KJ930492
der233 / T. granarium / USDA / X / 2 / USDA / KJ930493
der234 / T. granarium / USDA / X / 2 / USDA / KJ930494
der236 / T. inclusum / USDA / X / 1 / USDA / KJ930495
der239 / T. glabrum / USDA / X / 1 / USDA / KJ930496
der241 / T. sternale sp / R.L.O.O. / X / 5 / MSU / KJ930497
der243 / T. variable / R.L.O.O. / X / 1 / MSU / KJ930498
der244 / T. ornatum / R.L.O.O. / X / 1 / MSU / KJ930499
der247 / T. inclusum / R.L.O.O. / X / 1 / MSU / KJ930500
der248 / T. anthrenoides / R.S.B. / X / 1 / CPGAM / KJ930501
der249 / T. okumurai / R.S.B. / X / 1 / CPGAM / KJ930502
der250 / T. ornatum / R.S.B. / X / 1 / CPGAM / KJ930503
der251 / T. sternale / R.S.B. / X / 6 / CPGAM / KJ930504
der252 / T. sternale aspericolle / R.S.B. / X / 7 / CPGAM / KJ930505
der253 / T. teukton / R.S.B. / X / 1 / CPGAM / KJ930506
der254 / T. simplex / R.L.O.O. / X / 2 / MSU / KJ930507
der259 / T. glabrum / R.L.O.O. / X / 1 / MSU / KJ930508
der262 / T. sternale plagifer / R.S.B. / X / 8 / CPGAM / KJ930509
der270 / T. aspericolle / R.S.B. / X / 1 / CPGAM / KJ930510
der271 / T. teukton / R.L.O.O. / X / 1 / CPGAM / KJ930511
der277 / T. glabrum / R.L.O.O. / X / 1 / MSU / KJ930512
der278 / T. okumurai / R.L.O.O. / X / 2 / MSU / KJ930513
der279 / T. glabrum / R.L.O.O. / X / 1 / MSU / KJ930514
der280 / T. sternale plagifer / R.L.O.O. / X / 9 / MSU / KJ930515

Note: Identifier initials refer to: Michael Caterino (M.C.), Rachel Olson (RLOO, R.S. Beal, Jr. (R.S.B.), Alicia Bray (A.B.), Dr. Natalia Vandenberg at the USDA Agricultural Research Service (USDA)

Supplementary Table S3: 16S sequence data for each unique haplotype (refer to Table 1 for haplotype groupings) and primer and probe alignment.

T.species probe(P3gen) ACGCTGTTATCCCTAAGGTAATT

T.gran 1 CGCAATCTCTCCTTTCGATAAGAACTCTCTAGAAGAATTACGCTGTTATCCCTAAGGTAAT

T.gran 2 CGCAATCTCTCCTTTCGATAAGAACTCTCTAGAAGAATTACGCTGTTATCCCTAAGGTAAT

T.gran 3 CGCAATCTCTCCTTTCGATAAGAACTCTCTAGAAGAATTACGCTGTTATCCCTAAGGTAAT

T.incl 1 CGCAAACTCTCCTTTCGATTAGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

T.simp 1CGCAAAATAAACTTTCGATAAGAACTCTCAAGTAAAATAACGCTGTTATCCCTAAGGTAAT

T.simp 2 CGCAAACTCTTCTTTCGATAAGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

T.simp 3 CGCAAACTCTTCTTTCGATAAGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

T.vari1 CGCAAACTTTTTTTTCGATAAGAACTCTCAAAAAAAATTACGCTGTTATCCCTAAGGTAAT

T.glab1 CGCAATCTCTCCTCTCGATAAGAACTCTCCAGAAGAATTACGCTGTTATCCCTAAGGTAAT

T.ster1 ------TCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.ster2 CGCAACCCCTTCTTTCGATAAGAACTCTCAAGAAAGATTACGCTGGTATCCCTAAGGTAAT

T.ster3 CGCAACCCCTTCTTTCGATAAGGACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.ster4 ------AAGATTACGCTGTTATCCCTAAGGTAAT

T.ster5 CGCAACCCCTTCTTTCGATAAGGACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.ster6 CGCAACCCCTTCTTTCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.ster7 CGCAACCCCTCCTTTCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.ster8 CGCAACCCCTTTTTTCGTTAAGGACTCTCAAAAAGGATTACGCTGTTATCCCTAAGGTAAT

T.ster9 CGCAACCCCTTTTTTCGATAAGAACTCTCAAAAAGGATTACGCTGTTATCCCTAAGGTAAT

T.teuk 1 CGCAAACTCTTCTTTCGATTTGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

T.fasc1 CGCAAACTCTTATTTCGATAGGAACTCTCAAAAAGAATTACGCTGTTATCCCTAAGGTAAT

T.fasc2 ------ACTCTCAAAAAGAATTACGCTGTTATCCCTAAGGTAAT

T.fasc3 CGCAAACTCTTATTTCGATAGGGACTCTCAAAAAGAATTACGCTGTTATCCCTAAGGTAAT

T.gras1 CGCAACCCCTTCTTTCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.gras 2 CGCAACCCCTTCTTTCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.orna1 CGCAACCCCTTCTATCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.orna2 CGCAACCCCTTCTATCGATAAGAACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.okum 1 CGCAACCCTCTCTTTCGATAAGGACTCTCTAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.okum 2 CGCAACCCTCTCTTTCGATAAGGACTCTCTAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.aspe1 CGCAACCCCTCCTTTCGATAAGGACTCTCAAGAAAGATTACGCTGTTATCCCTAAGGTAAT

T.anth1 ------AAGATTACGCTGTTATCCCTAAGGTAAT

M.spec1 CGCAAACTTCCCTTTCGATAAGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

M.spec2 CGCAAACTTTTCTTTCGATAAGAACTCTCAAGAAAAATTACGCTGTTATCCCTAAGGTAAT

T.gran 1 TTAATCTTATAATCACAA—GATGGATCATCTAATCATAAATCAATGTTTCAATAAAAGAAA

T.gran 2 TTAATCTTATAATCACAA—GATGGATCATCTAATCATAAATCAATGTTTCAATAAAAGAAA

T.gran 3 TTAATCTTATAATCACAA—GATGGATCATCTAATCATAAATCAATGTTTCAATAAAAGAAA

T.incl 1 TATCTCTTACAATCAAAAAAATTGGATCATATAATCATAAATAAATGTTTAAATAAAGGAA

T.simp 1TTAATCTTTAAATCAATA--AATGGATCAAAAAATCATAAATCAATGAAAAAAT-AAAGAA

T.simp 2 TTAATCTTATAATCGTAAATAACGGATCAATTAATCATAAATAAATGTAAAAATAAAAGAA

T.simp 3 TTAATCTTATAATCGTAAATAACGGATCAATCAATCATAAATAAATGTAAAAATAAAAGAA

T.vari1 TTTATCTTTTAATCACAAAAAATGGATCACTTTTCCATAAATCAATGTAAAAATAAAAAGA

T.glab1 TTACTCTTATAATCACAA—-AATGGATCAACTAATCATAAATCAATGTTTAAATAAAAGAA

T.ster1TTAGTCTTATAATCAAAAAAAAAGGATCAAATACTCAATTAAAATTGTATAAATAAAAAAA

T.ster2TTAATCTTATAATCAAAA-AAAAGGATCAAATACTCAATAAAAATTGTCTAAATAAAAAAA

T.ster3 TTAATCTTATAATCACAAAAAGTGGATCAAATTATCAAATAAATTTGTATTAATAAAAAAA

T.ster4 TTAATCTTATAATCAAANAAAAAGGATCAAATACTCAATAAAAATTGTCTAAATAAAAAAA

T.ster5 TTAATCTTATAATCACAAAAAGTGGATCAAATTATCAAATAAATTTGTATTAATAAAAAAA

T.ster6 TTAATCTTGTAATCACAAAAAATGGATCAAATAATCAATTAAATTTGTTCAAATAAAAAAA

T.ster7 TTAGTCTTATAATCACAAAAAATGGATCAAATGTTCAATTAAATTTGTTCAAATAAAAAAA

T.ster8 TTAATCTTATAATCACAAAAAATGGATCAAATATTCAATAAAACTTGTTTAAATAAAAAAA

T.ster9 TTAATCTTATAATCACAAAAAATGGATCAAATATTCAATAAAACTTGTTTAAATAAAAAAA

T.teuk 1 TATATCTTATAATCACAATCCGTGGATCATATAATCATAAATAAATGTAAAAATAAAGGAA

T.fasc1 TTTTTCTTCTAATCATAAAAAATGGATCATTCTCTCATAAATTAATGTTTAAATAAAAAAA

T.fasc2 TTTTTCTTCTAATCATAAAAAATGGATCATTCTCTCATAAATTAATGTTTAAATAAAAAAA

T.fasc3 TTTTTCTTCTAATCATAAAAAATGGATCATTCTCTCATAAATTAATGTTTAAATAAAAAAA

T.gras1 TTAATCTTATAATCACAAAAAATGGATCAAAAATTCAATTAAACTTGTTAAAATAAAAAAA

T.gras 2 TTAATCTTCTAATCACAAAAAATGGATCAAAAATTCAATTAAACTTGTTAAAATAAAAAAA

T.orna1 TTAATCTTATAATCACAAAATATGGATCACATACTCAATTAAATTTGTAAAAATAAAAAAA

T.orna2 TTAATCTTATAATCACAAAATATGGATCACATACTCAATTAAATTTGTAAAAATAAAAAAA

T.okum 1 TTAATCTTATAATCACAAAAAATGGATCAAATGCCCAATTAAAATTGTCCCCATAAAAAAA

T.okum 2 TTAATCTTATAATCACAAAAAATGGATCAAATGCCCAATTAAAATTGTCCCCATAAAAAAA

T.aspe1 TTAGTCTTATAATCACAAAAAATGGATCAAATGTTCAATTAAATTTGTTCAAATAAAAAAA

T.anth1 TTAATCTTATAATCACAAAATATGGATCACATACTCAATTAAATTTGTAAAAATAAAAAAA

M.spec1 TTAATCTTTAAATCAAAACTTTTGGATCAAAAAATCATAAATAAATGTAAAAATAAAAAGA

M.spec2 TAACTCTTATAATCAAAAATATTGGATCAAAAACTCATAAATCAATGTAAAAATAAAAAGA

der16SF4 CTAAAATTGAAAATTTCTATACT

T.gran 1 AGTTAAATAAATTTTCTAGTCACCCCAACCAAATTAACCTAAA-ATTGAAAA-TTCTATAC

T.gran 2AGTTAAATAAATTTTCTAGTCACCCCAACCAAATTAACCTAAA-ATTGAAAA-TTCTATAC

T.gran 3 AGTTAAATAAATTTTCTAGTCACCCCAACCAAATTAACCTAAA-ATTGAAAA-TTCTATAC

T.incl 1 AAGTTAATTAAATTTTCCAATCACCCCAACCAAATTGCCTTTAACATTAAAAA-TTAAATA

T.simp 1 AAGTTTATTTAATTTTCTAGTCACCCCAACCAAATTAAGCTTTAATTAAAAACCATAATAC

T.simp 2 AAGTTAAATAAATTTTCCGATCACCCCAACCAAATTATTTTACATTTTAAGAA-TCTATTA

T.simp 3 AAGTTAAATAAATTTTCCAATCACCCCAACCAAATTATTTTACATCTTAAGAA-TATATTA

T.vari1 AAGTTTTTTTAATTTCCCAATCACCCCAACCAAATTACCTTTAATATTAAAAA-TTTAATT

T.glab1AAGTTAAATAAATTTTCCAATCACCCCAATCAAATTAACCCAAA-GTTGAAAA-CTCGATT

T.ster1 AAGTTAACTAAATTTTTCAATCACCCCAACCAAATTTTTCTAAATATTCAAAC-TTAAATA

T.ster2 AAGTTAACTAAATTTTTCAGTCACCCCAACCAAATTTTCCCAAATTTTTAAAC-TTAAATA

T.ster3 AAGTTTAATCAATTTTTCAATCACCCCAATTAAATTTTAAAAATTCATAAAAA-TTAAATA

T.ster4 AAGTTAACTAAATTTTTCAGTCACCCCAACCAAATTTTCCCAAATTTTTAAAC-TTAAATA

T.ster5 AAGTTTAATCAATTTTTCAATCACCCCAATTAAATTTTAAAAATTCATAAAAA-TTAAATA

T.ster6 AAGTTAAATCAATTTTTCAATCACCCCAATCAAATTTCTCCAAATCGTAAAAT-TTAAATA

T.ster7 AAGTTAAATCAATTTTTCAATCACCCCAATTAAATTTTTCCCAAATGTAAAAT-TTTAATA

T.ster8 AAGTTAAACCAATTTTTCAATCACCCCAACCAAATTTTCCAAATTCATGAAAC-TAAAATA

T.ster9 AAGTTAAACCAATTTTTCAATCACCCCAACCAAATTTTCCAAATTCATGAAAC-TAAAATA

T.teuk 1 AAGTTCAATCAATTTTCCAGTCACCCCAACTAAATTGTTTCTAACATTGAAAG-CTAAATA

T.fasc1 AAGTTAATTAAATTTTTTAGTCACCCCAACTAAATTAAAAAATAAATTAACAA-ATAAATA

T.fasc2 AAGTTAATTAAATTTTTTAGTCACCCCAACTAAATTAAAAAATAAATTAACAA-ATAAATA

T.fasc3 AAGTTAATTAAATTTTTTAGTCACCCCAACTAAATTAAAAAATAAATTAACAA-ATAAATA

T.gras1 AAGTTAAACCAATTTCTCAATCACCCCAACCAAATTCCTACAAATCACAAAAC-ATAAATA

T.gras 2 AAGTTAAACCAATTTCCCAATCACCCCAACCAAATTCCTACAAATCACAAAAC-ATAAATA

T.orna1 AAGTTTAATCAATTTTTCAATCACCCCAATCAAATTTTCAAAATTCATAAAAT-TTAAATA

T.orna2 AAGTTTAATCAATTTTTCAATCACCCCAATCAAATTTTCAAAATTCATAAAAT-TTAAATA

T.okum 1 AAGTTTTATCAATTTTTCAATCACCCCAATTAAATTTTCACAACTCACATAAA-ACAAATA

T.okum 2 AAGTTTAATCAATTTTTCAATCACCCCAATTAAATTTTCACAACTCACATAAA-ACAAATA

T.aspe1 AAGTTAAATCAATTTTTCAATCACCCCAATTAAATTTTCCCCAAATGTAAAAT-TTAAATA

T.anth1 AAGTTTAATCAATTTTTCAATCACCCCAATCAAATTTTCAAAATTCATAAAAT-TTAAATA

M.spec1 AAGTTAAATAAATTTCTCAATCACCCCAATCAAATCTATTCTAATATTAAAAT-ACAACAA

M.spec2 AAGTTAAATAAATTTCTTAATCACCCCAACTAAATTTAATATAATATTAAAAT-ACACATT

T.gran 1TAACAATTTAACAACTAAAGAAATAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAAA

T.gran 2 TAACAATTTAACAATTAAAGAAATAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAAA

T.gran 3 TAACAATTTAACAATTAAAGAAATAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAAA

T.incl 1 CCAAAATTCTATAAAAACAA----CAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.simp 1TAATTAACAAATTAAAAAAAACAAAAATAAAACTCTATAGGGTCTTCTCGTCTTTCACTAA

T.simp 2 CCAAAACTCAATAAAAATTAAA--TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAACAA

T.simp 3 CCAAAACTCAATAAAAATTAAA--TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAACAA

T.vari1 CCAAAATTTTATAAAT-CAAG---TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.glab1 CAATCAATCTAACAACCAAAGAAATAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster1 CTTAAAATAAAAAATATTCCAA--AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster2 CCTAAAATAAAAAATATTCCAAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster3 CCTAAAATTAATAAAAATTTCAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster4 CCTAAAATAAAAAATATTCCAAA-AAATAAAACTCTATNGGGTCTTCTCGTCTTTTAAAAA

T.ster5 CCTAAAATTAATAAAAATTTCAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster6 CCTAAAATTAATACAAAAAAGAAAAAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster7 CTTAAAATTAACACAAAAAAGAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster8 CCTAAAATAAACATAAAAAAAAGAAAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.ster9 CCTAAAATAAACATAAAAAAAGA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.teuk 1 CCAAAACCTTACAAATACAA----CAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAATA

T.fasc1 CCAAAAATGTACAAAAAACCTTT-TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.fasc2 CCAAAAATGTACAAAAAACCTTT-TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.fasc3 CCAAAAATGTACAAAAAACCTTT-TAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.gras1 CCAAAAATTAACATAAACTGCAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.gras 2 CCAAAAATTAACATAAACTGCAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.orna1 CCTAAAATTAATAAAAATCTAA--AAATAAAACTCTATAGGGTCTTCTCGTCTTTCAAAAA

T.orna2 CCTAAAATTAATAAAAATCCAA--AAATAAAACTCTATAGGGTCTTCTCGTCTTTCAAAAA

T.okum 1 CCTAAAATTAATGAAAACCGCAA-AAATCAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.okum 2 CCTAAAATTAATGAAAACCGTAA-AAATTAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.aspe1 CTTAAAACTAACACAAAAAAGAA-AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

T.anth1 CCTAAAATTAATAAAAATCTAA--AAATAAAACTCTATAGGGTCTTCTCGTCTTTCAAAAA

M.spec1 CCATACATATATAATAAAAAAT--AAATAAAACTCTATAGGGTCTTCTCGTCTTTTAAAAA

M.spec2 CCAAATACTTATAATATTAAAT--AAATAAAACTCTGTAGGGTCTTCTCGTCTTTTAAAAA

T.gran 1ATTTGAGCCTTTTAA-CTCAAAAGTAAAATTCATCAAAA-TAAAAAAGAGACAGTAATCAT

T.gran 2ATTTGAGCCTTTTAA-CTCAAAAGTAAAATTCATCAAAA-TAAAAAAGAGACAGTGATCAT

T.gran 3ATTTGAGCCTTTTAA-CTCAAAAGTAAAATTCATCAAAA-TAAAAAAGAGACAGTAATCAT

T.incl 1AATTTGAGCCTTTTTACTCAAAAGTCAAATTCACAAATA-TAGGGAAGAGACAGTAATTAT

T.simp 1 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAAATAAAATAATTAAGAGACAAAATATTC

T.simp 2 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAACAAAA-TTAGAAAGAGACAGAACTTTT

T.simp 3 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATAAAA-TTAGAAAGAGACAGAACTTTT

T.vari1 AATTTGAGCTTTTTAACTCAAAAATAAAATTCAATTAAA-TAGGAGAGAGACAGTAGTTAT

T.glab1 AATTTGAGCCTTTTAACTCAAAAGTAAAATTCACCAAAA-TAAAAAAGAGACAGTAATTAT

T.ster1 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATTTA--TCAAAAAGAGACAGTCATTTT

T.ster2 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATTTA--TCAAAAAGAGACAGCCATTTT

T.ster3 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCATCTTAA-TTAAAAAGAGACAGTTATTTT

T.ster4 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATTTA--TCAAAAAGAGACAGCCATTTT

T.ster5 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCATCTTAA-TTAAAAAGAGACAGTTATTTT

T.ster6 AATTTAAGCTTTTTAACTTAAAAATAAAATTCATTACAA-TTAAAAAGAGACAGTCAATTT

T.ster7 AATCTAAGCTTTTTAACTTAAAAATAAAATTCATTGTAA-TTAAAAAGAGACAGCTGATTT

T.ster8 AATTTAAGCTTTTTTACTTAAAAATAAAATTCATTTAAA-TTAAAAAGAGACAGTTATTTT

T.ster9 AATTTAAGCTTTTTTACTTAAAAATAAAATTCATTTAAA-TTAAAAAGAGACAGTTATTTT

T.teuk 1 AATTTGAGCCTTTTAACTCAAAAGTAAAATTCATATAAA-TAAGGGGGAGACAGAAACTAT

T.fasc1 AATTTGAGCCTTTTAACTCAAAAGTTAAATTCAGATAAA-TTAAAAAGAGACAGCACCCTT

T.fasc2 AATTTGAGCCTTTTAACTCAAAAGTTAAATTCAGATAAA-TTAAAAAGAGACAGCACCCTT

T.fasc3 AATTTGAGCCTTTTAACTCAAAAGTTAAATTCAGATAAA-TTAAAAAGAGACAGAACCCTT

T.gras1 AATTTGAGCTTTTTAACTCAAAAATAAAATTCATCCTAA-TCAAAAAGAGACAGTTATTTT

T.gras 2 AATTTGAGCTTTTTAACTCAAAAATAAAATTCATTCTAA-TCAAAAAGAGACAGTTATTTT

T.orna1AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAACCAAA-TCAGAAAGAGACAGTCATTTT

T.orna2 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATCAAA-TCAGAAAGAGACAGTCATTTT

T.okum 1 AATTTAAGCTTTTTAACTTAAAAATAAAATTCATCACAA-TCAAAAAGAGACAGTAATTTT

T.okum 2 AATTTAAGCTTTTTAACTTAAAAATAAAATTCATCACAA-TCAAAAAGAGACAGTAATTTT

T.aspe1 AATCTAAGCTTTTTAACTTAAAAATAAAATTCATTGTAA-TTAAAAAGAGACAGCAGGTTT

T.anth1 AATTTAAGCCTTTTAACTTAAAAGTAAAATTCAATCCCAATCAGAAAGAGACAGTCATTTT

M.spec1 AATTTAAGCCTTTTAACTCAAAAGTAAAATTCAAAAAC--TAAAAAAGAGACAGACTTTAT

M.spec2 AATTTTAGCCTTTTAACTAAAAAGTAAAATTCAAAAAT--TCAAAAAGAGACAGATTTTTT

T.G p2.1 3’ TGGTAAGTAAGGTCAAGGGT 5’

T.gran 1 TTCGTCCAACCATTCATTCCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.gran 2 TTCGTCCAACCATTCATTCCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.gran 3 TTCGTCCAACCATTCATTCCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.incl 1 CTCGTCCAACCATTCATTCCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.simp 1 CTCGTCCAATCATTCATTCCAGCTTACAATTAATAAACTAATTATTATGCTACCTTTGCA

T.simp 2 CTCGTCCAACCATTCATACCAGTTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.simp 3 CTCGTCCAACCATTCATACCAGTTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.vari1 CTCGTCCAACCATTCATTCCAGTTCCCAATTAAAAAACTAATTATTATGCTACCTTCGCA

T.glab1 TTCGTCCAACCATTCATTCCAGTTCCCAATAAAGGAACTAATTATTATGCTACCTTCGCA

T.ster1 CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTTGCA

T.ster2CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.ster3 CTCGTCCAACCATTCATACCAGTTTCCAATTAAAAAACTAATTATTATGCTACCTTCGCA

T.ster4 CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.ster5 CTCGTCCAACCATTCATACCAGTTTCCAATTAAAAAACTAATTATTATGCTACCTTCGCA

T.ster6 CTCGTCCAACCATTCATACCAGTTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.ster7 CTCGTCCAGCCATTCATACCAGTTACTAATCAAGTAACTAATTATTATGCTACCTTCGCA

T.ster8 CTCGTCCAACCATTCATTCCAGCTCCCAATAAAAGAACTAATTATTATGCTACCTTCGCA

T.ster9 CTCGTCCAACCATTCATTCCAGCTCCCAATAAAAGAACTAATTATTATGCTACCTTCGCA

T.teuk 1 CTCGTCCGACCATTCATTCCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.fasc1 CTCGTCAAATCGTTCATACCAGATCCTAATTAAGAACCTAATTATTATGCTACCTTCGCA

T.fasc2 CTCGTCAAATCATTCATACCAGATCCTAATTAAGAACCTAATTATTATGCTACCTTTGCA

T.fasc3 CTCGTCAAATCATTCATACCAGATCCTAATTAAGAACCTAATTATTATGCTACCTTTGCA

T.gras1 CTCGTCCAGCCATTCATTCCAGTTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.gras 2 CTCGTCCAGCCATTCATTCCAGTTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.orna1 CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.orna2 CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

T.okum 1 CTCGTCTAGCCATTCATTCCAGCTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.okum 2 CTCGTCCAGCCATTCATTCCAGCTCCCAATCAAGAAACTAATTATTATGCTACCTTCGCA

T.aspe1 CTCGTCCAGCCATTCATACCAGTTACTAATCAAGTAACTAATTATTATGCTACCTTCGCA

T.anth1 CTCGTCCAACCATTCATACCAGTTCCCAATAAAGAAACTAATTATTATGCTACCTTCGCA

M.spec1 CTCGTCAAACCATTCATACTAGATTCCAATAAAAAAACTAATTATTATGCTACCTTCGCA

M.spec2 CTCGTCCAACCATTCATACCAGTTCTCAATAAAAAAACTAATTATTATGCTACCTTCGCA

der16SR1 3’ AGTTAGTCCCTCGTCCGATC 5’

T.gran 1CAGTCAAAATACTGCGGCTCTTAAAATCAATCAGGGAGCAGGCTAGACCTTATATAAA

T.gran 2CAGTCAAAATACTGCGGCTCTTAAAATCAATCAGGGAGCAGGCTAGACCTTATATAAA

T.gran 3 CAGTCAAAATACTGCGGCTCTTAAAATCAATCAGGGAGCAGGCTAGACCTTATATAAA

T.incl 1 CAGTCAAAATACTGCGGCTCTTAAAAACC-TCAGGGAGCAGGCCAGACTTTAAATAAA

T.simp 1 CAGTCAAAATACTGCGGCCCTTCAACTAA-TCAGCGGGCAGATTAAACCTTATATAAT

T.simp 2 CAGTCAAAATACTGCGGCTCTTTAATTCA-TCAGTGAGCAGACCAGACTTTAAATAAA

T.simp 3 CAGTCAAAATACTGCGGCTCTTTAATTTA-TCAGTGAGCAGACCAGACTTTAAATAAA

T.vari1 CAGTCAAAATACTGCGGCTCTTAAACACC-TCAGGGAGCAGGCTAGACTTTAAATAAA

T.glab1 CAGTCAAAATACTGCGGCTCTTAAAAAAA-TCAGGGAGCAGGTTAGACCTTATATAAA

T.ster1 CAGTCAAAATACCGCGGCTCTTAAATTAC-TCAGGGAGCAGACCAGACTTTAAATAAA

T.ster2 CAGTCAAAATACTGCGGCTCTTAAATCAC-TCAGGGAGCAGACCAGACTTTAAATAAA

T.ster3 CAGTCAAAATACTGCGGCTCTTTAAAAA—-TCAGTGAGCAGACCAGACCTTATATAAA

T.ster4 CAGTCAAAATACTGCGGCTCTTAAATCAC-TCAGGGAGCAGACCAGACTTTAAATAAA

T.ster5 CAGTCAAAATACTGCGGCTCTTTAAAAA--TCAGTGAGCAGACCAGACCTTATATAAA

T.ster6 CAGTCAAAATACTGCGGCTCTTTAAATT--TCAGTGAGCAGACCAGACTTTAAATAAA

T.ster7 CAGTCAAAATACTGCGGCTCTTTAAATT--TCAGTGAGCAGACCAGACTTTAAATAAA

T.ster8 CAGTCAAAATACTGCGGCTCTTAAAACT—-TCAGTGAGCAGGCCAGACCTTAAATAAA

T.ster9 CAGTCAAAATACTGCGGCTCTTAAAACT--TCAGTGAGCAGGCCAGACCTTAAATAAA

T.teuk 1 CAGTCAAAATACTGCGGCTATTAAATACC-TCAGGGAGCAGGCCAGACTTTTAATAAA

T.fasc1 CGGTCAAAATACCGCGGCTCTTTAAACT--TCAGTGAGCAGATCAGACTTTAAAAAAA

T.fasc2 CGGTCAAAATACCGCGGCTCTTTAAACT--TCAGTGAGCAGATCAGACTTTAAAAAAA

T.fasc3 CGGTCAAAATACCGCGGCTCTTTAAACT--TCAGTGAGCAGATCAGACTTTAAAAAAA

T.gras1 CAGTCAAAATACTGCGGCTCTTAAAAAA--TCAGTGAGCAGACCAGACTTTAAATAAA

T.gras 2 CAGTCAAAATACTGCGGCTCTTAAAAAA--TCAGTGAGCAGACCAGACTTTAAATAAA

T.orna1 CAGTCAAAATACTGCGGCTCTTTAAAAC--TCAGTGAGCAGACCAGACTTTATATAAA

T.orna2 CAGTCAAAATACTGCGGCTCTTTAAAAC--TCAGTGAGCAGACCAGACTTTATATAAA

T.okum 1 CAGTCAAAATACTGCGGCTCTTAAAAAT--TCAGTGAGCAGACCAGACTTTAAATCAA

T.okum 2 CAGTCAAAATACTGCGGCTCTTAAAAAT--TCAGTGAGCAGACCAGACTTTAAATTAA

T.aspe1 CAGTCAAAATACTGCGGCTCTTTAAATT--TCAGTGAGCAGACCAGACTTTAAATAAA

T.anth1 CAGTCAAAATACTGCGGCTCTTTAAAAC--TCAG------

M.spec1 CGGTCAAAATACCGCGGCTCTTCAAATCC-TCAGTGAGCAGGTCAGACTTTAAATCAA

M.spec2 CGGTCAAAATACCGCGGCTCTTCAATTAA-TCAGCGAGCAGGCCAGACCTTAAATTAA

Supplemental Table S4. Real time PCR results for samples included in the study using P1 + P3 probes. CT values for Probe 3 and 1 are provided, the difference between these values, estimated concentration of DNA templates, and assay results. P1 CT values reported as failed indicate that no product was measured in the 45 cycles tested. The results are reported as positive, negative or inconclusive (P3 CT>30).

Taxon / Code / Result / Ct P3 / Ct P1 / Ct diff (P3-P1) / ng/µl
Trogoderma sternale sp / der30 / Negative / 24.69 / 39.23 / -14.54
Megatoma sp. / der31 / Negative / 27.85 / failed / 32
Megatoma sp. / der32 / Negative / 25.21 / failed / 63
Trogoderma granarium / der33 / Positive / 19.86 / 19.47 / 0.39 / 94
Trogoderma granarium / der38 / Positive / 25.27 / 24.22 / 1.05
Trogoderma granarium / der39 / Positive / 21.3 / 20.34 / 0.96
Trogoderma granarium / der40 / Positive / 19.68 / 19.25 / 0.43 / 67
Trogoderma granarium / der41 / Positive / 23.21 / 21.56 / 1.65 / 68
Trogoderma granarium / der42 / Positive / 23.61 / 21.96 / 1.65 / 40
Trogoderma granarium / der43 / Positive / 21.11 / 20.44 / 0.67 / 19
Trogoderma granarium / der44 / Positive / 21.11 / 21.17 / -0.06 / 26
Trogoderma granarium / der47 / Positive / 26.04 / 21.61 / 4.43 / 158
Trogoderma granarium / der48 / Positive / 25.2 / 22.38 / 2.82 / 35
Trogoderma granarium / der55 / Positive / 27.49 / 27.17 / 0.32
Trogoderma granarium / der63 / Positive / 27.75 / 26.35 / 1.4
Trogoderma granarium / der64 / Positive / 22.92 / 22.04 / 0.88 / 40
Trogoderma variable / der68 / Negative / 27.58 / 38.5 / -10.92 / 32
Trogoderma simplex / der71 / Inconclusive / 31.51 / 37.46 / -5.95 / 23
Trogoderma variable / der72 / Negative / 28.73 / 39.79 / -11.06 / 40
Trogoderma granarium / der80 / Positive / 25.43 / 23.75 / 1.68 / 33
Trogoderma granarium / der83 / Positive / 22.08 / 21.29 / 0.79
Trogoderma granarium / der84 / Positive / 24.02 / 22.91 / 1.11
Trogoderma granarium / der85 / Positive / 27.39 / 25.05 / 2.34
Trogoderma granarium / der86 / Positive / 21.4 / 20.72 / 0.68 / 62
Trogoderma granarium / der88 / Inconclusive / 33.06 / 30.8 / 2.26 / 44
Trogoderma granarium / der89 / Positive / 25.22 / 22.64 / 2.58 / 51
Trogoderma granarium / der91 / Inconclusive / 31.24 / 28.26 / 2.98 / 30
Trogoderma inclusum / der94 / Negative / 19.78 / 39.46 / -19.68 / 63
Trogoderma simplex / der98 / Negative / 20.17 / 38.82 / -18.65
Trogoderma inclusum / der104 / Negative / 29.31 / 41.05 / -11.74 / 60
Trogoderma glabrum / der105 / Negative / 27.24 / 41.2 / -13.96 / 211
Trogoderma glabrum / der106 / Negative / 20.78 / 40.98 / -20.2 / 215
Trogoderma sternale sternale / der116 / Negative / 16.77 / 40.93 / -24.16 / 95
Trogoderma simplex / der118 / Negative / 22.44 / 43.31 / -20.87 / 39
Trogoderma grassmani / der131 / Negative / 17.17 / 37.69 / -20.52
Trogoderma simplex / der132 / Negative / 20.98 / failed / 72
Megatoma sp. / der138 / Negative / 17.61 / failed
Trogoderma sternale sp / der175 / Negative / 18.7 / 38.32 / -19.62
Trogoderma variable / der176 / Negative / 17.89 / 40.94 / -23.05
Trogoderma teukton / der177 / Negative / 20.23 / 43.05 / -22.82 / 30
Trogoderma inclusum / der180 / Negative / 23.47 / failed / 27
Trogoderma grassmani / der184 / Negative / 25.41 / 39.31 / -13.9 / 30
Trogoderma fasciferum / der193 / Negative / 27.06 / 38.62 / -11.56 / 62
Trogoderma variable / der194 / Negative / 19.94 / 42.68 / -22.74
Trogoderma ornatum / der196 / Negative / 24.24 / failed / 67
Trogoderma fasciferum / der199 / Negative / 22.86 / 40.29 / -17.43 / 37
Trogoderma fasciferum / der200 / Negative / 22.47 / 41.73 / -19.26 / 42
Trogoderma sternale sternale / der201 / Negative / 23.53 / failed
Trogoderma ornatum / der202 / Negative / 21.52 / 38.58 / -17.06 / 21
Trogoderma simplex / der211 / Negative / 20.08 / failed / 20
Trogoderma granarium / der216 / Positive / 27.5 / 23.55 / 3.95 / 29
Trogoderma granarium / der217 / Positive / 21.91 / 19.45 / 2.46 / 20
Trogoderma granarium / der219 / Positive / 29.59 / 27.12 / 2.47 / 82
Trogoderma granarium / der220 / Positive / 21.4 / 19.8 / 1.6 / 26
Trogoderma granarium / der221 / Positive / 19.84 / 18.89 / 0.95 / 64
Trogoderma granarium / der222 / Positive / 24.48 / 21.53 / 2.95 / 11
Trogoderma granarium / der223 / Positive / 19.67 / 17.45 / 2.22 / 91
Trogoderma granarium / der224 / Positive / 28.42 / 23.24 / 5.18 / 60
Trogoderma granarium / der225 / Inconclusive / 33.38 / 28.91 / 4.47 / 52
Trogoderma granarium / der226 / Inconclusive / 34.55 / 28.29 / 6.26 / 69
Trogoderma granarium / der228 / Positive / 26.18 / 23.63 / 2.55 / 76
Trogoderma granarium / der229 / Positive / 22.75 / 20.16 / 2.59 / 41
Trogoderma granarium / der230 / Positive / 26.46 / 23.75 / 2.71 / 107
Trogoderma granarium / der231 / Positive / 23.18 / 20.28 / 2.9 / 24
Trogoderma granarium / der232 / Positive / 24.68 / 23.25 / 1.43 / 43
Trogoderma granarium / der233 / Positive / 29.77 / 30.23 / -0.46 / 22
Trogoderma granarium / der234 / Inconclusive / 30.7 / 29.16 / 1.54 / 34
Trogoderma inclusum / der236 / Inconclusive / 31.36 / 38.37 / -7.01
Trogoderma glabrum / der239 / Negative / 20.33 / 42.43 / -22.1 / 27
Trogoderma sternale sp / der241 / Negative / 22.68 / 39.71 / -17.03
Trogoderma variable / der243 / Negative / 13.21 / 41.54 / -28.33 / 23
Trogoderma ornatum / der244 / Negative / 17.49 / 40.47 / -22.98 / 35
Trogoderma inclusum / der247 / Negative / 26.26 / failed / 29
Trogoderma anthrenoides / der248 / Not Tested
Trogoderma okumurai / der249 / Negative / 23.26 / failed / 36
Trogoderma ornatum / der250 / Negative / 16.64 / 41.89 / -25.25 / 21
Trogoderma sternale plagifer / der251 / Negative / 18.51 / 39.57 / -21.06
Trogoderma sternale aspericolle / der252 / Negative / 26.05 / failed / 120
Trogoderma teukton / der253 / Inconclusive / 31.68 / 35.37 / -3.69 / 72
Trogoderma simplex / der254 / Negative / 23.13 / failed / 20
Trogoderma glabrum / der259 / Negative / 26.53 / 43.98 / -17.45 / 23
Trogoderma sternale plagifer / der262 / Negative / 20.36 / failed / 30
Trogoderma aspericolle / der270 / Negative / 26.93 / failed
Trogoderma teukton / der271 / Negative / 25.74 / 40.16 / -14.42 / 60
Trogoderma glabrum / der277 / Negative / 23.44 / failed
Trogoderma okumurai / der278 / Negative / 24.22 / failed / 59
Trogoderma glabrum / der279 / Negative / 24.27 / 39.32 / -15.05 / 30
Trogoderma sternale plagifer / der280 / Negative / 23.11 / failed

1