Supplementary Table 1ANOVA of the effect of temperature and pH (both fixed factors) on percentage of a. fertilisation, b. cleavage, c. hatching, d. gastrulation and e. abnormal embryos of the New Zealand and Australian populations ofCentrostephanus rodgersii. Levene's test p-values for each developmental stage and each site are 0.095 for New Zealand and 0.109 for Australia (fertilisation), 0.394 for New Zealand and 0.210 for Australia (cleavage), 0.183 for New Zealand and 0.300 for Australia (hatching), 0.429 for New Zealand and 0.272 for Australia (gastrulation), and 0.905 for New Zealand and 0.067 for Australia (abnormal embryos).

Stage / Population / Source / SS / df / F / p
a. Fertilisation / New Zealand / Temperature / 0.889 / 1 / 7.762 / 0.192
pH / 125.903 / 3 / 366.596 / <0.001
Temperature:pH / 0.894 / 3 / 2.603 / 0.075
Residuals / 2.748 / 24
Australia / Temperature / 0.324 / 1 / 3.143 / 0.089
pH / 236.498 / 3 / 765.899 / <0.001
Temperature:pH / 0.181 / 3 / 0.587 / 0.629
Residuals / 2.470 / 24
b. Cleavage / New Zealand / Temperature / 147.870 / 1 / 643.768 / <0.001
pH / 346.870 / 3 / 503.396 / <0.001
Temperature:pH / 0.730 / 3 / 1.059 / 0.371
Residuals / 20.210 / 88
Australia / Temperature / 2.270 / 1 / 7.092 / <0.01
pH / 996.100 / 3 / 1038.091 / <0.001
Temperature:pH / 0.1900 / 3 / 0.198 / 0.898
Residuals / 28.150 / 88
c. Hatching / New Zealand / Temperature / 154.820 / 1 / 353.304 / <0.001
pH / 427.000 / 3 / 324.806 / <0.001
Temperature:pH / 13.160 / 3 / 10.011 / <0.001
Residuals / 38.560 / 88
Australia / Temperature / 3.700 / 1 / 13.749 / <0.001
pH / 754.880 / 3 / 934.193 / <0.001
Temperature:pH / 7.78 / 3 / 9.631 / <0.001
Residuals / 23.70 / 88
d. Gastrulation / New Zealand / Temperature / 1.387 / 1 / 6.696 / <0.05
pH / 280.649 / 3 / 451.659 / <0.001
Temperature:pH / 0.048 / 3 / 0.078 / 0.972
Residuals / 18.227 / 88
Australia / Temperature / 2.200 / 1 / 10.043 / <0.01
pH / 1135.420 / 3 / 1730.456 / <0.001
Temperature:pH / 2.650 / 3 / 4.038 / <0.01
Residuals / 19.250 / 88
e. Abnormal embryos / New Zealand / Temperature / 8.978 / 1 / 12.903 / 0.001
pH / 92.910 / 3 / 44.510 / <0.001
Temperature:pH / 0.816 / 3 / 0.391 / 0.760
Residuals / 61.230 / 88
Australia / Temperature / 17.685 / 1 / 41.905 / <0.001
pH / 138.864 / 3 / 109.681 / <0.001
Temperature:pH / 1.864 / 3 / 1.472 / 0.228
Residuals / 37.138 / 88

1