SYLLABUSOF
M. Phil.
PLANT BIOTECHNOLOGY
GENETIC ENGINEERING
(w.e.f. JUNE – 2010)
Re-Accredited Grade 'B' by NAAC(CGPA 2.93)
DEPARTMENT OF BIOSCIENCES
SAURASHTRA UNIVERSITY
RAJKOT – 360 005
DEPARTMENT OF BIOSCIENCES
SAURASHTRA UNIVERSITY
M. PHIL. SYLLABUS
CBCS System
May, 2010
The M. Phil syllabus in this Department is now converted to choice based CBCS system. There will be two semesters with one theory course in each. Each theory course shall be of 80 marks but there will not be any practicals. The Dissertation will be spread over the two semesters and account for 200 marks. Though it will commence in the beginning of the first semester, it will be evaluated and grade points will be given with the second and final semester. This CBCS based M. Phil syllabus will be applicable from current academic session of 2010.
Thus, the M. Phil Programmes being run in the Department of Biosciences will be having the following structure:
Programme : M. Phil
Duration : 2 Semesters
Courses : 1 in each Sem. Core course in 1st Sem. and Elective in 2nd Sem.
Credit : Each paper will be of 4 credits in 4 h/week/Sem. 08 Credits
Seminar / Assignment course 1 credit in 1h/w/Sem 02
Dissertation: 15 hours/week/Sem. 10 Credits
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Total 20 Credits
Marks : Each paper 80 marks (2 Papers) 160 Marks
Dissertation 200 Marks
Seminar / Assignment(1 in each Sem) 40 Marks
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Total 400 Marks
Examination Pattern:
- The Semester end Theory examination will comprise of four questions each of 20 marks from each Unit of the syllabus with 100% internal option.
- Seminar / Assignment will be evaluated based on the Seminar or presentation made by them.
- Dissertation will be evaluated by both, external referee and Viva-voce examination in the Department, in consultation with the Dissertation Supervisor. For this purpose out of allocated 200 marks for dissertation, 150 marks will be for the evaluation of M. Phil. Thesis and 50 marks for the Viva-voce by the External Referee and Internal Dissertation Supervisor.
99
M.Phil. Syllabus
Plant Biotechnology & Genetic Engineering
CHOICE BASED CREDIT SYSTEM (CBCS)
(Total 20 Credits)
COURSE / PAPER NAME / HOURS / WEEK / CREDIT / MARKSSEMESTER - I
M.Phil.PBGE-101 / Course – 1 / 4 / 4 / 80
M.Phil.PBGE -102 / Assignment & Seminar Course / 1 / 1 / 20
M.Phil.PBGE -103 / Dissertation – I* / 15 / 5
SEMESTER TOTAL / 20 / 10 / 100
SEMESTER - II
M.Phil.PBGE -201 / Course – 2 / 4 / 4 / 80
M.Phil.PBGE -202 / Assignment & Seminar Course / 1 / 1 / 20
M.Phil.PBGE -103 / Dissertation – II* / 15 / 5 / 200
SEMESTER TOTAL / 20 / 10 / 300
GRAND TOTAL / 40 / 20 / 400
*Dissertation will commence in the beginning of the first Semester but will be evaluated and grade points will be given in the Final Semester.
Semester – I
Course -101
Unit – 1: Cell & Molecular Biology
1.1Cell and its Organelles-Structure and Function
1.2Plant Cell Membrane Ultrastructure and Function
1.3Basic Component of Plant Cell Wall
1.4Mechanism of Cell Elongation
1.5Regulation of Cellular Metabolism
1.6Enzyme and Enzyme Kinetics
1.7DNA Mutations, Repair and Recombination
1.8 Chromosomal Aberration and Polyploidy
Unit – 2 : Plant Hormones & its Regulation
2.1Phytohormones and Plant Growth Regulators
2.2Synthetic and Natural Plant Growth Regulators
2.3Role of Phytohormones in Growth and Development
2.4 Mode of Action and Regulation (Auxin, Gibberellins, Cytokinins, Abscisic Acid, Ethylene, Brassinosteroids, Jasminoids etc.)
2.5 Signal Transduction (Definition and Overview, Receptors, Second Messengers and mechanism of action)
2.1Phytohormones and Its Applications in Plant Biotechnology
Unit – 3 : Immunological Techniques
3.1Immune System and Types of Immunity
3.2Antigen-Antibody Types, Structure and Function
3.3Generation of Immune Diversity
3.4Basic Techniques for Plant Immunology
3.5Hybridoma Technology
3.6Plantibody
3.7 Plant Diagnostics-Disease Screening and Identification of Plant Variety
Unit – 4 : Computer & Bioinformatics
4.1Introduction to Bioinformatics
4.2Overview of Standard Operation and Application Softwares
4.3Data Analysis and Presentation
4.4General Awareness to Popular Softwares in Plant Biology
4.5Biological Data Bank
4.6 Sequence Alignment and Homology
Semester – II
Course – 201
Unit: 1 Plant Propagation Techniques
1.1Choice of Propagation Methods
1.2Propagation Structure, Equipment and Media
1.3Seed and Vegetative Propagation
1.4Layering, Grafting and Budding Techniques
1.5Bonsai Techniques
1.6Greenhouse Technology
1.7Hydroponics
1.8Wormiculture and Biofertilizers
1.9Biological Control of Pests
1.10Factors Regulating Propagation
Unit: 2 Tissue Culture Techniques
2.1Basics of Plant Tissue Culture
2.2Plant Material Selection
2.3Culture Medium Selection, Preparation and Manipulation
2.4Material Preparation
2.5Callus Induction and Subculturing
2.6Protoplast Isolation, Culture and Selection of Hybrids
2.7Anther Culture and Production of Haploids
2.8Organ Culture and Organogenesis
2.9Preparation of Artificial Seeds
2.10Somaclonal Variation
2.11Production of Secondary Metabolites
Unit: 3 Genetic Engineering in Plants
3.1Evolution of Genetic Engineering
3.2 Basic techniques and Instrumentation related with genetic engineering: DNA and RNA Isolation, Gene Mapping and Blot Techniques (Northern, Southern etc.), PCR and DNA Fingerprinting Techniques (RAPD, AFLP etc.), Sequencing
3.3cDNA
3.4Gene Cloning Techniques
3.5Protein Isolation and Purification
3.6Agrobacterium-mediated transformation
3.7Microprojectile bombardment-mediated transformation and Target tissue preparation, Microcarrier preparation and DNA delivery
Unit: 4 Legal Issues in Plant Biotechnology
4.1Overview of Intellectual Property
4.2IPR: Indian and Global Scenario
4.3Related International Treaties and Agreements
4.4Patent : Criteria of Patentability, Indian Patent Act, Patenting of Biological Material
4.5Plant Variety Protection
4.6Farmer’s and Breeder’s Rights
4.7Biodiversity Act
4.8 Protection of Traditional Knowledge