CURRICULUM VITAE
Name : B. ARISTATILE
Sex : Male
Date of Birth : 03.01.1980
Nationality : Indian
Qualification : M.Sc., M.Phil., Ph.D.
Address for Communication : Department of Food Science and Nutrition,
CollegeofFoodand Agricultural Science,
KingSaudUniversity,
P.O. Box2460,
Riyadh11451,
Saudi Arabia.
Tel no:+9661467-9094.
Fax:+9661467-8394.
Permanent address : 454 New street
Puliyur Post- 607 301
Panruti(Tk)
Cuddalore (Dis)
Tamil Nadu. India.
Ph.+91-4142-279149
Email :
Educational Qualifications : B.Sc. Biochemistry, 57 %
Madras University, 2002
: M.Sc. Biochemistry, First Class
Periyar University University, 2005
: M.Phil. Biochemistry, First Class
Annamalai University, 2008
: Ph.D. Biochemistry,
Bharathiar University, (Likely to submit thesis)
Field of Research : Hepatotoxicity
Areas of Interest : Molecular mechanism and phytochemicals treatment in the prevention of hyperlipidemia and herbal medicine.
RESEARCH EXPERIENCE : Five years of research experience as M.Phil. & Ph.D Research Scholar in Annamalai University, India.
Working experience : Worked as a Biochemist in Department of Biochemistry, Sri Venkatashwara Medical College & Research Centre, Pondicherry. duration 2005-2006 ,
: Working as a researcher in King saud University. 2008 to till date
Title of M.Phil Thesis
Effect of Carvacrol on galctosamine induced hepatotoxicity in rats.
Title of Ph.D.Thesis
Hepatoprotective efficacy of carvacrol in D-GalN induced rats and its inhibitory effect on inflammatory markers.
Abstract
Carvacrol (2-mehtyl-5-(1-methylethyl)-phenol) is a predominant monoterpenic phenol which occurs in many essential oils of the family Labiatae including, Origanum, Satureja, Thymbra, Thymus, and Corydothymus species. In our earlier study we investigated the hepatoprotective activity of carvacrol, using D-galactosamine (D-GalN) induction as a model of hepatotoxicity at three different doses (20,40 and 80mg/kg body weight). D-GalN- hepatotoxic rats exhibited elevation in hepatic marker enzymes such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transpeptidase, Oral administration of carvacrol for 21 days reversed these parameters towards normalcy. The biochemical observations were supported by histological examination of rat liver and kidney tissue. The 20 mg/kg body wt. dose of the compound produced a better effect than 40 or 80 mg doses. These results suggest that carvacrol could afford a significant hepatoprotective effect against D-GalN induced rats.
Since it is 20 mg dose i.e. the lower dose, that showed better activity we would now like try lower doses. Further, using optimum dose we have proposed to investigate the lipid profile, lipidperoxidative markers such as thiobarbituric acid reactive substances and lipid hydroperoxides and the activities of enzymatic antioxidant enzymes superoxide dismutase, catalase, glutathione peroxides, and nonenzymatic antioxidants (vitamin C, vitamin E and reduced glutathione) in the plasma, erythrocytes and tissues in carvacrol- treated GalN-induced hepatoxic rats. We will examine PPAR-alpha and gene expression by RT-PCR and the inflammatory markers such as COX-2 & TNF-α protein expression by western blotting analysis upon carvacrol treatment. We will also investigate the localization of cytokines such as IL-6 and cytochrome P450 by immunohistochemical analysis in the liver after carvacrol treatment. These results will be compared with silymarin the standard drug used for hepatotoxicity.
Technical Expertise
Working knowledge :
Ø In Biochemistry
SDS-PAGE
Immunoelectrophoresis
Agarose gel electrophoresis
Ø IN CLINICAL BIOCHEMISTRY
Small Animal handling
Lipid peroxidation studies
Antioxidants and Detoxifying enzymes
Various Enzyme parameters
Ø In Molecular Biology
DNA and RNA isolation techniques
PCR and RT-PCR technique
Western Blot technique
INSTRUMENTS KNOWN TO HANDLE :
Spectrophotometer, Auto analyzers, Spectroflurimeter, ELISA Reader, TLC, HPTLC, HPLC.
Membership in Professional Bodies
1. Society of Biological Chemists of India
2. Society for Free Radical Research – India (SFRR- India)
3. Indian Society of Atherosclerosis Research (ISAR)- India
4. Indian Science Congress, India
Workshops Attended
1. Participated UGC Sponsored National Level Workshop on ‘Herbal Drug Standardization’ held in the Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University during 19 & 20 February 2005.
2. Participated in International training course on ‘ICRO – International Cell Research Organisation’ organized by K.S.R College of Arts and Science held on 7-20 Sep 2003
PUBLICATIONS
1. Balakrishnan Aristatile, Khalid S. Al- Numair, Chinnadurai Veeramani and Kodukkur Viswanathan Pugalendi. Effect of carvacrol on hepatic marker enzymes and antioxidant status in D-galactosamine-induced hepatotoxicity in rats. Fundamental & Clinical PharmacologyVol. 23, 757–765. 2009.
2. Balakrishnan Aristatile, Khalid S. Al- Numair,§ Chinnadurai Veeramani and Kodukkur Viswanathan Pugalendi. Antihyperlipidemic effect of carvacrol on d-galactosamine-induced hepatotoxic rats. Journal of Basic & Clinical Physiology & Pharmacology Vol. 20, No. 1, 2009
3. Balakrishnan Aristatile, Khalid S. Al- Numair, Abdullah. H. Al – Assaf Chinnadurai Veeramani and Kodukkur Viswanathan Pugalendi. Protective effect of carvacrol on oxidative stress and cellular DNA damage induced by UVB irradiation in human peripheral lymphocytes. Journal of Biochemical and Molecular Toxicology (In Press).
4. C. Veeramani, B. Aristatile G. Pushpavalli, K.V. Pugalendi. Antihypertensive effect of Melothria maderaspatana leaf extracts on uninephrectomized DOCA-salt hypertensive rats. Basic and Clinical Physiology and Pharmacology 2010: Volume 22.
5. C. Veeramani, B. Aristatile G. Pushpavalli, K.V. Pugalendi. Antioxidants efficacy of Melothria maderaspatana leaf extracts on uninephrectomized DOCA-salt hypertensive rats. Saudi Journal of Biological Sciences, 18 (2011) 99-105
6. Balakrishnan Aristatile, Khalid S. Al- Numair, Abdullah. H. Al – Assaf and Kodukkur Viswanathan Pugalendi. Pharmacological effect of the carvacrol on D-galactosamine-induced mitochondrial enzymes and DNA damage by single-cell gel Electrophoresis.Experimental and Toxicologic Pathology, (Under Review).
7. Balakrishnan Aristatile, Khalid S. Al- Numair, Abdullah. H. Al – Assaf and Kodukkur Viswanathan Pugalendi. Protective effect of carvacrol on inflammatory markers in D-galactosamine--induced hepatotoxicity rats. Chemico-Biological Interactions, (Under Review).
Conference/Seminar Paper presented
1. International Conference on Natural Products in Health and ISARCON-2008 held during 8-10 December, 2008 in the department of Biochemistry and Biotechnology, Annamalai University, Tamil Nadu, India. (Oral presentation)
2. CSIR-ICMR-TNSCST-Sponsored National Conference on Diabetes Mellitus and Cancer (DIA-CAN’ 09) 19 & 20 th December, 2009 held in the Department of Biochemistry & Biotechnology, Annamalai University, Tamil Nadu, India.
3. Participated in 94th Indian Science Congress organized by Annamalai University, Annamalainagar held on 3-7 Jan 2007.
4. National /Conference on Free Radical and Antioxidants in Health, Annamalai University, Annamalainagar, Tamil Nadu, held on March 2-4, 2007. (Oral presentation).
5. Participated in UGC Sponsored National Workshop/Conference on ‘Free radicals and antioxidants in health’ held at Annamalai University, Annamalainagar, Tamil Nadu on March 2 – 4, 2007.
6. Participated in National conference on Daiabetes and Cancer (Dia-Can 07) December 15th & 16th, 2007, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Chidambaram.
Reference
1. Dr. K.V Pugalendi (Research Supervisor) 2. Dr. Venugopal P. Menon
Professor and Head Research Director
Dept. of Biochemistry & Annamalai University,
Biotechnology Annamalainagar-608 002
Annamalai University, Tamil Nadu, India.
Annamalainagar-608 002 Email:
Tamil Nadu, India.
Email:
3. Dr. Kanagavalli
Professor and Head
Sri Venkateshwaraa Medical College and research Centre
Pondicherry, India.
Ph. 0413 – 2644435