(%) / Hypocotyllargerthan 1 cm (%) / Cotyledonslargerthan 1 cm (%)
0 / 15 / 100 a / 0 a / 0 a / 0 a
3 / 15 / 100 a / 0 a / 0 a / 0 a
6 / 15 / 100 a / 66.6 b / 0 a / 53.3 b
10 / 15 / 93.3 b / 73.3bc / 53.3 b / 66.6 b
14 / 15 / 93.3 b / 80 d / 80 c / 86.6 c
21 / 15 / 86.6 c / 86.6 d / 80 c / 86.6 c
ABA / Days of culture / Number of explants / Survival (%) / Germination (%) / Hypocotyllargerthan 1 cm (%) / Cotyledonslargerthan 1 cm (%)
0 / 15 / 100 a / 0 a / 0 a / 0 a
3 / 15 / 93.3 b / 0 a / 0 a / 0 a
6 / 15 / 93.3 b / 13.3 b / 0 a / 0 a
10 / 15 / 93.3 b / 86.6 c / 86.6 b / 80 b
14 / 15 / 93.3 b / 93.3 d / 86.6 b / 86.6bc
21 / 15 / 93.3 b / 93.3 d / 93.3 c / 93.3 c
BA / Days of culture / Number of explants / Survival (%) / Adventitiousshootinduction (%) / Basal callus
(%) / Cotyledonslargerthan 1 cm (%)
0 / 15 / 100 a / 0 a / 0 a / 0 a
3 / 15 / 100 a / 0 a / 86.6 b / 0 a
6 / 15 / 100 a / 0 a / 86.6 b / 0 a
10 / 15 / 93.3 b / 40 b / 86.6 b / 0 a
14 / 15 / 86.6 c / 66.6 c / 86.6 b / 20 b
21 / 15 / 86.6 c / 80 d / 86.6 b / 73.3 c
IBA / Days of culture / Number of explants / Survival (%) / Indirectrootinduction (%) / Basal callus
(%) / Cotyledonslargerthan 1 cm (%)
0 / 15 / 100 a / 0 a / 0 a / 0 a
3 / 15 / 100 a / 0 a / 0 a / 0 a
6 / 15 / 100 a / 0 a / 0 a / 0 a
10 / 15 / 100 a / 66.6 b / 93.3 b / 6.66 a
14 / 15 / 100 a / 100 c / 100 b / 33.4 b
21 / 15 / 100 a / 100 c / 100 b / 93.3 c
2,4-D / Days of culture / Number of explants / Survival (%) / Celldedifferentiation (%) / Basal callus
(%) / Cotyledonslargerthan 1 cm (%)
0 / 15 / 100 a / 0 a / 0 a / 0 a
3 / 15 / 93.3 b / 0 a / 26.6 b / 0 a
6 / 15 / 93.3 b / 53.3 b / 53.3 c / 0 a
10 / 15 / 93.3 b / 93.3 c / 93.3 d / 0 a
14 / 15 / 93.3 b / 93.3 c / 93.3 d / 13.3 b
21 / 15 / 93.3 b / 93.3 c / 93.3 d / 13.3 b
SupplementaryTable S2: Macromorphological response of P. radiata embryos/seedlings cultured in basal medium QLP, and in basal medium QLP plus growth regulators BA, ABA, IBA and 2,4-D (22.2 µM). Different letters indicate significant differences by Tuckey test after ANOVA D= 0.01%.