Heat Treatment Induced Bacterial Changes in Irrigation Water and Their Implications For

Heat treatment induced bacterial changes in irrigation water and their implications for plant disease management

W. Hao* and C. X. Hong

Hampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, Virginia 23455, USA

*Corresponding author. Tel: +1 (757) 363-3941, fax: +1 (757) 363-3950, email:

Submitted to World Journal of Microbiology and Biotechnology

Table S2 Alignment of bacterialpartial sequences of 16S rDNAfrom water samples collected in February and May 2012 by selected cultures and excised DGGE bands

Sequence deposit / Closest match (Accession number)a
Sequence ID (Accession #) / Size (bp) / Similarity (%) / Frequency
February 2012 – selected cultures
251A1_P28_C01 (JX870915) / 304 / 100 / 6 / AcidovoraxdelafieldiiA3R07 (JQ689177)
251A2_P17_C04 (JX870916) / 300 / 100 / 3 / Pelomonassp. UKW24 (JQ687102)
251A5_P32_E06 (JX870917) / 303 / 99 / 4 / XenophilusaerolatusNML 100527 (JN585330)
251C4_P14_E08 (JX870922) / 300 / 100 / 5 / RheinheimeratexasensisJN-9 (JF496548)
251E1_P38_A09 (JX870924) / 301 / 100 / 2 / Bacillus cereus CT25 (EU111736)
251E4_P38_B09 (JX870925) / 304 / 100 / 3 / Bacillus pumilus TAD088 (FJ225317)
251E7_P30_H04 (JX870926) / 304 / 100 / 6 / Bacillus marisflavi BVC64 (JQ407799)
251E8_P38_C09 (JX870927) / 300 / 100 / 3 / Bacillus sp. DHC04 (JQ904715)
252D3_P31_H05 (JX870935) / 303 / 99 / 5 / Pseudomonas sp. HM05 (JQ396178)
253F8_P28_E02 (JX870961) / 302 / 100 / 1 / Bacillus vietnamensisKL2-3 (AB697709)
421A1_P38_A03 (JX870979) / 302 / 100 / 4 / Bacillus pumilus TAD088 (FJ225317)
421C6_P23_H10 (JX870966) / 305 / 99 / 12 / Pseudomonas aeruginosa NTS6 (HE858286)
422A7_P25_F01 (JX870985) / 299 / 100 / 2 / Bacillus pumilus JR5-4 (JQ229695)
422D2_P35_F12 (JX870972) / 300 / 100 / 5 / ExiguobacteriumprofundumCIBA BW17 (JQ073758)
423A2_P38_B04 (JX870991) / 299 / 100 / 2 / Bacillus sp. PZ_27 (JQ808526)
423A8_P38_C04 (JX870992) / 298 / 100 / 3 / Bacillus pumilus SRS83 (JQ965500)
423C2_P38_E04 (JX870996) / 302 / 100 / 7 / Bacillus cereus CT25 (EU111736)
423F8_P7_H02 (JX871001) / 303 / 100 / 13 / Pseudomonas sp. NBRC 101713 (AB681538)
481A5_P38_A09 (JX871002) / 299 / 100 / 4 / Bacillus sp. PZ_27 (JQ808526)
481A8_P37_F05 (JX871003) / 303 / 100 / 6 / Pseudomonas sp.R3S4-1(JQ928687)
481C6_P38_B09 (JX871005) / 303 / 100 / 15 / Bacillus cereus CT25 (EU111736)
481D1_P30_B07 (JX871006) / 300 / 99 / 3 / Bacillus marisflaviBVC64 (JQ407799)
481E3_P38_E09 (JX871013) / 304 / 99 / 8 / Bacillus sp. s-bf-PMW-5.1.1 (FR774585)
482B6_P38_A10 (JX871019) / 298 / 100 / 3 / Paenibacillus sp. 140SI (JF825471)
482F4_P38_G10 (JX871031) / 298 / 100 / 3 / Bacillus cibiIARI-S-22(JN411495)
483A4_P25_A07 (JX871035) / 301 / 100 / 2 / Paenibacillus terrigena B30 (JN252080)
483A6_P35_D05 (JX871036) / 298 / 100 / 2 / Paenibacillus sp. BL16-1 (EU912455)
483D6_P38_A12 (JX871042) / 302 / 100 / 4 / Bacillus pumilus TAD088 (FJ225317)
483E6_P38_D12 (JX871045) / 300 / 100 / 4 / Bacillus sp. cmc28 (JQ917994)
February 2012 – DGGE
1A5_25_A09 (JX657295) / 377 / 99 / 1 / Uncultured Actinobacterium clone KWK1S.54 (JN656761)
1A7_25_C09 (JX657296) / 381 / 99 / 2 / Uncultured Bacteroidetes bacterium clone 30LAKE43F10 (HQ531074)
1B1_25_E09 (JX657298) / 374 / 99 / 1 / UnculturedPolynucleobacter sp. clone YL205 (HM856566)
1B2_25_F09 (JX657299) / 366 / 99 / 1 / UnculturedHerbaspirillumsp. clone FL_52 (HQ008597)
1B4_25_H09 (JX657300) / 375 / 99 / 1 / Uncultured Actinobacterium clone KWK6F.62 (JN656787)
1C1_25_B10 (JX657301) / 378 / 100 / 2 / Uncultured Actinobacterium clone w98 (HE654991)
1D1_25_D10 (JX657303) / 378 / 100 / 1 / Uncultured Actinobacterium clone w70 (HE654972)
1D2_25_E10 (JX657304) / 378 / 99 / 1 / Uncultured bacterium clone ncd2310d03c1 (JF198212)
1D5_25_G10 (JX657306) / 377 / 99 / 2 / Uncultured Mycobacterium sp. clone AMPE9 (AM935142)
1D8_25_H10 (JX657307) / 380 / 100 / 1 / Uncultured Sphinogobacterium sp. clone w21 (HE654931)
1E4_25_B11 (JX657309) / 375 / 100 / 2 / Uncultured Actinobacterium clone w60 (HE654966)
1E5_25_C11 (JX657310) / 375 / 99 / 1 / Uncultured bacterium clone CRP99-73 (AF428727)
1F1_25_D11 (JX657311) / 379 / 99 / 1 / Uncultured Mycobacterium sp. clone AMPE9 (AM935142)
1F4_25_E11 (JX657312) / 379 / 100 / 2 / Uncultured bacterium clone BST15-17 (HQ436607)
1G3_25_H11 (JX657314) / 378 / 98 / 1 / UnculturedRhodobactersp. clone rm15b3c02 (HM003636)
1H6_25_F12 (JX657315) / 377 / 99 / 1 / Uncultured α-Proteobacterium clone DC5-50-1 (AY145582)
2C1_42_E03 (JX871138) / 377 / 99 / 1 / Uncultured bacterium clone 261ds10 (AY212711)
2C2_42_F03 (JX871139) / 377 / 99 / 1 / Pseudomonas jessenii strain E62 (JF683673)
2C3_42_G03 (JX871140) / 377 / 99 / 2 / Uncultured bacterium clone CR98-5-65 (AF428795)
2D1_42_B04 (JX871142) / 382 / 100 / 1 / Uncultured Sphinogobacteriales bacterium clone W-LLR195 (HE654931)
2D3_42_D04 (JX871143) / 382 / 99 / 1 / Uncultured bacterium clone Lc2z_ML_201 (FJ355341)
2E1_42_E04 (JX871144) / 382 / 99 / 1 / Uncultured bacterium clone reservoir-169 (JF697550)
2E5_42_F04 (FX871145) / 382 / 98 / 1 / Uncultured bacterium clone nbw497f10c1 (GQ101655)
2E7_42_G04 (JX871146) / 382 / 100 / 1 / Microvirgaflocculans strain TFB (JF438969)
2F2_42_H04 (JX871147) / 382 / 100 / 1 / Pseudomonas aeruginosa strain NTS6 (HE858286)
2F5_42_A05 (JX871148) / 382 / 100 / 1 / Uncultured bacterium clone R242 (HM069056)
2G2_42_C05 (JX871149) / 382 / 99 / 1 / Uncultured bacterium clone C24.47 (GU559802)
2G3_42_D05 (JX871150) / 377 / 98 / 1 / Pseudomonas sp. Cf0-5 (JF683673)
2H3_42_G05 (JX871151) / 377 / 100 / 1 / Uncultured bacterium clone CR98-5-65 (AF428795)
2I1_42_B06 (JX871152) / 377 / 99 / 1 / Uncultured bacterium clone ML-3-55 (DQ166460)
2I3_42_D06 (JX871153) / 376 / 99 / 1 / Hydrogenophagasp. WLSH-321 (JF682011)
2I6_42_G06 JX871154) / 376 / 100 / 1 / Hydrogenophagasp. p3(2011) (HQ652595)
2J1_42_A07 (JX871155) / 382 / 96 / 1 / Uncultured α-Proteobacteria bacterium clone QEDV2BC02 (JQ696175)
2J5_42_D07 (JX871156) / 372 / 100 / 1 / Uncultured α-Proteobacterium clone DC5-50-1 (AY145582)
3C2_48_D09 (JX871157) / 372 / 100 / 1 / Uncultured bacterium clone SINN828 (HM128813)
3C7_48_F09 (JX871158) / 375 / 99 / 1 / Uncultured bacterium clone TSBAR002 (AB486314)
3D8_48_H09 (JX871159) / 376 / 98 / 2 / UnculturedBalneimonassp. clone UHAS4.3 (JN037960)
3E1_48_A10 (JX871160) / 383 / 97 / 1 / Chitinophaga sp. BS27 (JF806525)
3E5_48_B10 (JX871161) / 383 / 97 / 1 / Uncultured bacterium clone 2N1-3 (EU160054)
3F5_48_E10 (JX871162) / 383 / 98 / 1 / Uncultured bacterium clone 5-12 (JQ923510)
3G2_48_H10 (JX871164) / 383 / 99 / 2 / Uncultured bacterium clone MFC_19D7 (JF204132)
3G8_48_C11 (JX871165) / 369 / 96 / 1 / Uncultured α-Proteobacteria bacterium clone QEDV2BC02 (JQ696175)
3H2_48_E11 (JX871166) / 354 / 99 / 1 / Uncultured bacterium clone Lc2z_ML_201 (FJ355341)
May 2012 – selected cultures
251A3_P2_F06 (JX628693) / 306 / 100 / 1 / Chitinibactersp. SK16 (JN981166)
251A5_P3_G07 (JX628694) / 306 / 100 / 4 / Hydrogenophagasp. p3(2011) (HQ652595)
251A8_P6_H08 (JX628695) / 306 / 100 / 1 / Microbacteriumsp. F1 1010 (JQ691553)
251B1_P7_C09 (JX628696) / 306 / 100 / 1 / Pseudomonas sp. MLB28 (JQ765424)
251B4_P9_A10 (JX628698) / 306 / 99 / 2 / CandidatusAzospirillummassiliensisstrain URAM1 (EF394925)
251B6_P11_F02 (JX628699) / 306 / 100 / 2 / Pseudomonas sp. NCCP-279 (AB641888)
251C4_P10_H01 (JX628701) / 301 / 99 / 3 / Pseudomonas sp. HM05 (JQ396178)
251D4_P15_D05 (JX628705) / 306 / 100 / 2 / Enterobactersp. X40 (HE662680)
251E4_P13_H03 (JX628706) / 306 / 100 / 6 / Bacillus sp. cmc28 (JQ917994)
251F7_P16_C06 (JX628709) / 306 / 100 / 4 / Bacillus mycoidesTN12 (JQ415984)
252G5_P8_H09 (JX628714) / 301 / 100 / 1 / Rheinheimerasp. E49 (JQ922424)
252H1_P6_B09 (JX628716) / 307 / 100 / 1 / Microbacteriumsp. E56 (JF501120)
252H6_P17_E06 (JX628718) / 305 / 100 / 1 / Pelomonassp. UKW24 (JQ687102)
252H7_P1_D01 (JX628719) / 301 / 100 / 1 / Acidovoraxsp. DPS2 (HQ704415)
252I1_P15_F05 (JX628720) / 306 / 100 / 2 / Mitsuariasp. Vi30 (JF501194)
252I2_P10_B02 (JX628721) / 301 / 100 / 1 / Azospirillumsp. DJM2D4 (JF753513)
253A4_P12_G03 (JX628733) / 308 / 99 / 1 / Mesorhizobiumsp. RITF 741 (JQ697665)
253A7_P1_E01 (JX628735) / 301 / 100 / 1 / Acidovoraxsp. NF1078 (JQ782387)
253F1_P13_F04 (JX628746) / 306 / 100 / 3 / Bacillus marisflavip53_B09 (JQ834634)
421G6_P2_H12 (JX871050) / 301 / 100 / 9 / Bacillus sp. cmc28 (JQ917994)
421I4_P18_C11 (JX871051) / 301 / 100 / 4 / Vogesellasp. TPS6 (FJ821602)
421L6_P20_F02 (JX871066) / 301 / 100 / 14 / Pseudomonas sp. J61 (JQ670671)
421L7_P19_A12 (JX871067) / 307 / 100 / 8 / Pseudomonas aeruginosa NTS6 (HE858286)
422E4_P11_B10 (JX871055) / 306 / 100 / 2 / Pseudomonas sp. K7 (FR874238)
423G7_P22_C03 (JX871073) / 308 / 100 / 2 / Pseudomonas sp. DCA-9 (FJ493141)
423H4_P4_A04 (JX871079) / 306 / 100 / 2 / Brevibacillus parabrevisIFO 12334 (NR_040981)
481B2_P24_G09 (JX871086) / 301 / 100 / 2 / Paenibacillus sp. DHC15 (JQ904726)
481C6_P12_C05 (JX871093) / 308 / 100 / 6 / Bacillus sp. PZ_7 (JQ808525)
481D8_P13_D0 (JX871096) / 306 / 100 / 2 / Bacillus sp. Pt1 (JF900600)
481E1_P26_F10 (JX871097) / 306 / 100 / 5 / Bacillus marisflavip53_B09 (JQ834634)
481E2_P13_E06 (JX871098) / 301 / 99 / 2 / Bacillus koreensisWIF49 (HM480320)
482G7_P25_A10 (JX871104) / 306 / 100 / 2 / Paenibacillus timonensis3584BRRJ (JF309265)
482H1_P6_H12 (JX871105) / 301 / 98 / 3 / Paenibacillus sp. Dv34 (HQ728085)
482H4_P2_G08 (JX871107) / 306 / 100 / 3 / Cohnellasp. CC-Alfalfa-35 (JN806384)
482I1_P5_C12 (JX871110) / 302 / 99 / 3 / Paenibacillus sp. KMC003 (EU219972)
482I5_P16_C08 (JX871112) / 306 / 100 / 3 / Paenibacillus sp. WYT011 (JQ807858)
482I6_P13_H06 (JX871113) / 303 / 100 / 3 / Bacillus sp. PZ_35 (JQ808528)
482K4_P2_H08 (JX871116) / 306 / 100 / 2 / Bacillus pumilusCE4 (JQ435701)
483A5_P26_D11 (JX871120) / 301 / 100 / 4 / Bacillus ginsengihumiANA15 (HQ219846)
483B6_P25_C10 (JX871123) / 306 / 100 / 4 / Paenibacillus sp. MN2-8 (JQ396530)
483B7_P27_F11 (JX871124) / 308 / 100 / 8 / Brevibacillus sp. SE12 (JQ714095)
483D6_P13_F07 (JX871132) / 301 / 100 / 7 / Bacillus sp. cmc28 (JQ917994)
483F1_P2_C09 (JX871134) / 306 / 100 / 2 / Bacillus sp. DHC04 (JQ904715)
May 2012 - DGGE
1A2_25_C01 (JX657316) / 411 / 100 / 1 / Uncultured bacterium clone SINN828 (HM128813)
1A4_25_D01 (JX657317) / 411 / 96 / 1 / Uncultured bacterium clone Lc2z_ML_055 (FJ355132)
1A5_25_E01 (JX657318) / 411 / 98 / 1 / Uncultured bacterium clone Filt_WallC02 (GQ247163)
1A8_25_F01 (JX657319) / 411 / 99 / 1 / Uncultured Actinobacteriumclone 30LAKE01B08 (HQ530574)
1B4_25_H01 (JX657320) / 411 / 99 / 2 / Uncultured bacterium clone JC43 (JN868893)
1B8_25_C02 (JX657322) / 411 / 99 / 1 / Uncultured bacterium clone 060127_T6S1_W_T_SDP_144 (FJ350708)
1C1_25_D02 (JX657323) / 420 / 99 / 1 / Uncultured Rheinheimera sp. clone 48PP (JF278072)
1C2_25_E02 (JX657324) / 420 / 99 / 1 / Uncultured bacterium clone S0052 (FJ820417)
1C3_25_F02 (JX657325) / 420 / 99 / 1 / Uncultured Polynucleobacter sp. clone YL205 (HM856566)
1C4_25_G02 (JX657326) / 420 / 99 / 1 / Uncultured bacterium clone S0052 (FJ820417)
1C5_25_H02 (JX657327) / 420 / 100 / 1 / Uncultured Bacteroidetes bacterium clone ME002C1 (FJ827913)
1C6_25_A03 (JX657328) / 420 / 99 / 1 / Uncultured bacterium clone S0052 (FJ820417)
1D3_25_C03 (JX657329) / 404 / 99 / 2 / Uncultured bacterium clone SINN1113 (HM128496)
1D5_25_D03 (JX657330) / 404 / 100 / 1 / Uncultured Rhodobactersp. clone rm15b3c02 (HM003636)
1E1_42_F03 (JX871167) / 406 / 100 / 1 / Uncultured bacterium clone L7S16_ML_056 (FJ354286)
1E2_42_G03 (JX871168) / 407 / 100 / 1 / Uncultured bacterium clone L7S16_ML_056 (FJ354286)
1E4_42_H03 (JX871169) / 408 / 99 / 1 / Uncultured bacterium clone JN59 (JN869004)
1E5_42_A04 (JX871170) / 408 / 97 / 1 / Uncultured α-Proteobacteria bacterium QEDN8CG03 (CU927557)
1F1_42_B04 (JX871171) / 408 / 99 / 1 / Uncultured bacterium clone nbw44h10c1 (GQ060166)
1F2_42_C04 (JX871172) / 408 / 99 / 1 / Uncultured Bacteroidetes bacterium TQQ6 (HE793031)
1F7_42_D04 (JX871173) / 408 / 99 / 1 / Pseudomonas aeruginosa NTS6 (HE858286)
1F8_42_E04 (JX871174) / 408 / 99 / 1 / Uncultured bacterium clone YHW13 (GU305837)
1G1_42_F04 (JX871175) / 408 / 99 / 1 / Uncultured bacterium clone MABRDTU30 (FJ529983)
1G2_42_G04 (JX871176) / 408 / 99 / 1 / Uncultured bacterium clone nbw44h10c1 (GQ060166)
1G3_42_H04 (JX871177) / 408 / 96 / 1 / Uncultured Methylosinussp. clone Plot18-F12 (FJ889302)
1G5_42_A05 (JX871178) / 417 / 97 / 1 / UnculturedFluviicola sp. clone JXS1-92 (JN873202)
1I2_42_G05 (JX871179) / 417 / 100 / 2 / Pseudomonas aeruginosa NTS6 (HE858286)
1I4_42_A06 (JX871181) / 417 / 97 / 1 / Uncultured α-Proteobacteria bacterium QEDN8CG03 (CU927557)
1I5_42_B06 (JX871182) / 401 / 96 / 1 / Uncultured Pseudomonas sp. clone GE7GXPU01AGHHE (HM973327)
1I7_42_C06 (JX871183) / 402 / 99 / 1 / Uncultured bacterium clone YHW13 (GU305837)
1I8_42_D06 (JX871184) / 403 / 96 / 2 / Uncultured Pseudomonas sp. clone F5OHPNU07H0UQZ (HQ053550)
1K2_42_B07 (JX871186) / 404 / 99 / 1 / Uncultured bacterium clone JN59 (JN869004)
1K3_42_C07 (JX871187) / 416 / 99 / 1 / Uncultured γ-Proteobacterium clone 30LAKE02G02 (HQ530658)
1K4_42_D07 (JX871188) / 416 / 100 / 1 / Uncultured bacterium clone 060127_T6S1_W_T_SDP_186 (FJ350717)
1K5_42_E07 (JX871189) / 416 / 96 / 1 / Uncultured α-Proteobacteria bacterium QEDN8CG03 (CU927557)
1K7_42_F07 (JX871190) / 416 / 99 / 1 / Uncultured bacterium clone 6-77 (JQ923731)
1K8_42_G07 (JX871191) / 416 / 99 / 1 / Uncultured bacterium clone 6-77 (JQ923731)
2A1_42_C08 (JX871192) / 416 / 99 / 1 / Uncultured bacterium clone JN59 (JN869004)
2A4_42_E08 (JX871193) / 408 / 99 / 1 / Uncultured α-Proteobacteria bacterium clone QEDV2BC02 (JQ696175)
2A6_42_G08 (JX871194) / 412 / 99 / 2 / Uncultured Azospirillum sp. clone GE7GXPU01AVTUJ (HQ044242)
2B2_42_B09 (JX871195) / 412 / 99 / 1 / Uncultured bacterium clone JN59 (JN869004)
2B8_42_D09 (JX871197) / 412 / 99 / 2 / Uncultured bacterium clone nbw44h10c1 (GQ060166)
2C2_42_E09 (JX871198) / 412 / 97 / 1 / Uncultured bacterium clone HK220 (FJ204047)
2C3_42_F09 (JX871199) / 412 / 99 / 1 / Uncultured bacterium clone nbw44h10c1 (GQ060166)
2C8_42_A10 (JX871200) / 412 / 99 / 1 / Uncultured bacterium clone nbw44h10c1 (GQ060166)
2E1_48_F10 (JX871201) / 403 / 99 / 1 / Uncultured Bacteroidetes bacterium clone 30LAKE43F10 (HQ531074)
2F1_48_H10 (JX871202) / 402 / 97 / 2 / Chitinophaga sp. BS27 (JF806525)
2G5_48_B11 (JX871204) / 403 / 97 / 1 / Uncultured Sphingomonadales bacterium clone SSF018 (JF733360)
2G7_48_C11 (JX871205) / 404 / 100 / 1 / Uncultured bacterium clone 14-66 (JF697368)
2G8_48_D11 (JX871206) / 412 / 99 / 1 / Lysobacter sp. YIM 77875 (JQ746036)
2H2_48_E11 (JX871207) / 414 / 98 / 1 / Uncultured Bacillus sp. clone SGR28 (JQ793404)
2H8_48_G11 (JX871208) / 412 / 99 / 1 / Uncultured bacterium clone SING1058 (HM128975)
2J3_48_A12 (JX871210) / 412 / 98 / 1 / Uncultured bacterium clone DP7.2.51 (FJ612260)
2J4_48_B12 (JX871211) / 412 / 98 / 1 / Uncultured bacterium clone ncd2158f12c1 (JF185564)
2J6_48_C12 (JX871212) / 412 / 98 / 1 / Uncultured bacterium clone DP7.2.51 (FJ612260)
2J7_48_D12 (JX871213) / 412 / 96 / 1 / Uncultured bacterium clone Fec185 (FJ911492)
2K3_48_F12 (JX871214) / 412 / 96 / 1 / Uncultured bacterium clone HK220 (FJ204047)
2K4_48_G12 (JX871215) / 412 / 99 / 1 / Uncultured bacterium clone SING1058 (HM128975)
2K6_48_H12 (JX871216) / 413 / 97 / 1 / Uncultured Bacillus sp. clone SGR28 (JQ793404)

aSequences aligned to their closest matches in GenBank using BLAST