Supplementary Table S1

Profile of sequences analyzed for selective pressure by hosts.

Host / Number / Year / Mean diversity
Human / 1217 / 1918-2008 / 0.050
Aviana / 1154 / 1902-2008 / 0.072
Swine / 123 / 1930-2006 / 0.069
Canine/Equine / 53 / 1963-2005 / 0.015

Footnote

a Chicken sequencing data were excluded.


Supplementary Table S2

Profile of human influenza sequences analyzed for selective pressure by time.

Temporal group / Number / Year / Mean diversity
Before 1965 / 62 / 1918-1965 / 0.023
After 1966 / 1157 / 1966-2008 / 0.050
Before 1999 / 440 / 1918-1999 / 0.038
After 2000 / 784 / 2000-2008 / 0.052


Supplementary Table S3

P-values for differential selective pressure on sites associated with amantadine resistance between hosts

A)

Human / Avian / Swine / Canine/Equine
Human / 0.54 / 0.16 / 1
Avian / 0.024a / 0.087 / 1
Swine / 0.79 / 0.093 / 1
Canine/Equine / 0.99 / 1 / 0.99

B)

Human / Avian / Swine / Canine/Equine
Human / 0.86 / 0.73 / 0.10
Avian / 0.70 / 0.50 / 0.063
Swine / 0.63 / 0.29 / 0.068
Canine/Equine / 0.036 a / 0.017 a / 0.057


C)

Human / Avian / Swine / Canine/Equine
Human / 0.20 / 0.31 / 1
Avian / 0.40 / 0.77 / 1
Swine / 0.95 / 0.45 / 1
Canine/Equine / 1 / 1 / 1

D)

Human / Avian / Swine / Canine/Equine
Human / 0.36 / 0.35 / 0.21
Avian / 0.089 / 0.77 / 0.31
Swine / 0.28 / 0.88 / 0.15
Canine/Equine / 0.075 / 0.16 / 0.16

Footnote

Results of eFEL and iFEL testing differential selective pressure between hosts for sites associated with amantadine resistance for (A) position 26, (B) position 27, (C) position 30, and (D) position 31, respectively.

Below left shows the P-values by eFEL and upper right shows P-values by iFEL.

a Statistically significant difference (P 0.05)