(A) (B) (C)
Fig.S1DGGE analysis obtained from PCR amplification of 16S rDNA V3 region with eubacterial primers 352T and 519r. DGGE profile for seedling stage (A), tillering stage (B) and maturity stage (C) in Dongjin and RS526 rice. DJ, Dongjin; RS526, Resveratrol GM rice 526 ; M, DGGE molecular weight marker; DJ1,DJ 2, andDJ 3 indicate individualDJ soil sample and DJ4 indicates mixed soil sample of DJ1, DJ 2, and DJ 3; RS1, RS 2, andRS 3 indicate individual RS526 soil sample and RS4 indicates mixed soil sample of RS1, RS2, andRS3
Table S1 Soil chemical properties in therhizosphere soil of Dongjin and RS526 rice
GrowthStage / Sample / pH / Pa
(mg/kg) / ECb
(dS/m) / T-Nc
(%) / OMd
(g/kg) / Cation(cmol/kg)
K / Ca / Mg / Na
Seedling
Stage / DJ / 6.4±0.02 / 71.0±0.59 / 0.3±0.00 / 0.1±0.00 / 20.4±0.16 / 0.6±0.01 / 7.6±0.17 / 1.5±0.01 / 0.2±0.00
RS526 / 6.3±0.01 / 71.3±0.18 / 0.3±0.00 / 0.2±0.00 / 21.2±0.49 / 0.6±0.00 / 7.1±0.13 / 1.4±0.00 / 0.2±0.01
Tillering
Stage / DJ / 6.5±0.01 / 55.4±1.24 / 0.3±0.00 / 0.2±0.00 / 21.2±0.64 / 0.5±0.01 / 7.4±0.11 / 1.6±0.01 / 0.1±0.00
RS526 / 6.3±0.01 / 52.8±0.72 / 0.3±0.00 / 0.2±0.00 / 20.0±0.21 / 0.4±0.01 / 6.7±0.13 / 1.5±0.00 / 0.2±0.00
Maturity
stage / DJ / 6.2±0.03 / 71.1±0.35 / 0.3±0.00 / 0.2±0.01 / 19.3±0.84 / 0.5±0.02 / 7.3±0.10 / 1.7±0.00 / 0.2±0.01
RS526 / 5.9±0.06 / 68.0±0.14 / 0.3±0.01 / 0.2±0.00 / 19.4±0.27 / 0.4±0.01 / 6.8±0.12 / 1.6±0.00 / 0.2±0.00
a, Available phosphate; b, Electrical conductivity; c, Total nitrogen; d, Soil organic matter
Table S2Average number of colony-forming units of therhizosphere soil
GrowthStage / Microbes / Dongjin / RS526
Seedling
Stage / Bacteria(x106) / 56±1.7 / 57.7±8.1
Fungi(x103) / 10.3±0.6 / 12.3±2.3
Tillering
Stage / Bacteria(x106) / 40.7±5.7 / 43.0±3.0
Fungi(x103) / 9.3±0.6 / 10.3±1.2
Maturity
stage / Bacteria(x106) / 36.7±4.5 / 33.0±4.4
Fungi(x103) / 11.7±0.6 / 7.7±2.5
Values indicate the colony forming unit(CFU)/g wet weight ± standard deviation from three replications.The asterisk denotes a significant difference (t-test, p0.05).