Table 3 - Descriptive Statistical Analysis of the Candidate Genes in Mouse Hemibrain At

Table 1 supplementary - Housekeeping genes used for gene expression studies on mouse brain regions

Brain
(n=101) / Cerebellum (n=70) / Brain Stem
(n=68) / Hippocampus
(n=76) / Septum
(n=12) / Neocortex
(n=18) / Olfactory bulb
(n=36)
Gapdh / 38.6% (n=39) / 34.3% (n=24) / 44.1% (n=30) / 47.4% (n=36) / 33.3% (n=4) / 50% (n=9) / 41.7% (n=15)
B-actin / 31.7% (n=32) / 37.1% (n=26) / 29.4% (n=20) / 36.8% (n=28) / 25% (n=3) / 33.3% (n=6) / 41.7% (n=15)
Hprt / 6.9% (n=7) / 7.1% (n=5) / 8.8% (n=6) / 6.6% (n=5) / 8.3% (n=1) / 11.1% (n=2) / 5.5% (n=2)
B2m / 3% (n=3) / 4.3% (n=3) / 5.9% (n=4)
Tbp / 1% (n=1)
B-tubulin / 2.6% (n=2)
18s / 19.8% (n=20) / 20% (n=14) / 16.2% (n=11) / 11.8% (n=9) / 25% (n=3) / 22.2% (n=4) / 8.3% (n=3)
28s / 4.3% (n=3) / 7.4% (n=5) / 8.3% (n=1)
16s / 1% (n=1) / 8.3% (n=1) / 5.5% (n=2)
5s / 1% (n=1) / 1.4% (n=1) / 2.9% (n=2)
40s / 1.4% (n=1) / 1.5% (n=1) / 1.3% (n=1)
14s / 2.8% (n=1)
39s / 1% (n=1)
Cyclophilin A / 1% (n=1) / 1.4% (n=1)
Cyclophilin B / 4% (n=4)
Pgk1 / 1.4% (n=1)

Table 2 supplementary - Descriptive statistical analysis of the candidate genes in mouse hemibrain at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 6.110·10-09 / 8.187·10-09 / 3.851·10-09 / 1.595·10-09 / 2.256·10-09 / 2.684·10-09 / 1.908·10-09 / 3.106·10-10
18 s / 1.235·10-04 / 1.663·10-04 / 6.801·10-05 / 4.294·10-05 / 2.038·10-05 / 2.830·10-05 / 1.323·10-05 / 5.423·10-06
Tfrc / 7.763·10-09 / 1.044·10-08 / 6.170·10-09 / 1.899·10-09 / 2.204·10-09 / 3.079·10-09 / 1.357·10-09 / 6.883·10-10
Gapdh / 1.035·10-07 / 1.653·10-07 / 6.410·10-08 / 3.834·10-08 / 5.795·10-08 / 7.197·10-08 / 4.212·10-08 / 1.379·10-08
Gusb / 2.314·10-09 / 2.997·10-09 / 1.237·10-09 / 6.555·10-10 / 4.433·10-10 / 5.409·10-10 / 3.754·10-10 / 6.173·10-11
B2m / 3.559·10-09 / 5.107·10-09 / 1.701·10-09 / 1.250·10-09 / 1.652·10-09 / 1.947·10-09 / 1.381·10-09 / 2.090·10-10

Table 3 supplementary -Descriptive statistical analysis of the candidate genes in mouse cerebellum at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 5.154·10-10 / 1.034·10-09 / 3.618·10-10 / 2.906·10-10 / 3.295·10-10 / 4.238·10-10 / 9.735·10-11 / 1.364·10-10
18 s / 3.178·10-06 / 8.489·10-06 / 7.123·10-07 / 3.092·10-06 / 1.732·10-06 / 2.812·10-06 / 2.525·10-07 / 9.344·10-07
Tfrc / 7.865·10-10 / 1.482·10-09 / 2.153·10-10 / 4.671·10-10 / 4.372·10-10 / 5.813·10-10 / 1.681·10-10 / 1.704·10-10
Gapdh / 8.959·10-09 / 1.520·10-08 / 5.398·10-09 / 3.967·10-09 / 7.381·10-09 / 1.302·10-08 / 3.034·10-09 / 4.930·10-09
Gusb / 2.312·10-10 / 4.794·10-10 / 1.250·10-10 / 1.417·10-10 / 6.562·10-11 / 9.077·10-11 / 3.141·10-11 / 2.173·10-11
B2m / 4.637·10-10 / 8.301·10-10 / 2.487·10-10 / 2.308·10-10 / 2.967·10-10 / 4.136·10-10 / 9.967·10-11 / 1.209·10-10

Table 4 supplementary -Descriptive statistical analysis of the candidate genes in mouse brain stem at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 3.883·10-10 / 6.636·10-10 / 2.195·10-10 / 1.770·10-10 / 5.955·10-10 / 1.230·10-09 / 1.340·10-10 / 4.279·10-10
18 s / 2.770·10-06 / 3.721·10-06 / 1.886·10-06 / 7.563·10-07 / 1.881·10-06 / 2.636·10-06 / 8.194·10-07 / 7.711·10-07
Tfrc / 6.901·10-10 / 7.963·10-10 / 5.519·10-10 / 8.861·10-11 / 4.806·10-10 / 8.267·10-10 / 2.074·10-10 / 2.459·10-10
Gapdh / 8.760·10-09 / 1.396·10-08 / 7.191·10-10 / 5.835·10-09 / 9.780·10-09 / 1.464·10-08 / 4.261·10-09 / 4.175·10-09
Gusb / 1.260·10-10 / 1.661·10-10 / 5.745·10-11 / 4.216·10-11 / 1.063·10-10 / 1.932·10-10 / 4.226·10-11 / 5.625·10-11
B2m / 2.936·10-10 / 8.382·10-10 / 6.308·10-11 / 3.131·10-10 / 3.085·10-10 / 6.856·10-10 / 7.317·10-11 / 2.450·10-10

Table 5 supplementary -Descriptive statistical analysis of the candidate genes in mouse hippocampus at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 2.037·10-10 / 3.468·10-10 / 5.064·10-11 / 1.238·10-10 / 3.794·10-10 / 8.823·10-10 / 1.345·10-10 / 2.947·10-10
18 s / 1.555·10-06 / 2.543·10-06 / 3.323·10-07 / 8.500·10-07 / 1.618·10-06 / 3.616·10-06 / 3.667·10-07 / 1.207·10-06
Tfrc / 3.120·10-10 / 7.745·10-10 / 5.492·10-11 / 2.719·10-10 / 4.358·10-10 / 1.076·10-09 / 1.883·10-10 / 3.641·10-10
Gapdh / 5.095·10-09 / 1.235·10-08 / 1.079·10-09 / 4.393·10-09 / 1.010·10-08 / 2.351·10-08 / 4.483·10-09 / 7.824·10-09
Gusb / 6.091·10-11 / 1.004·10-10 / 9.769·10-12 / 3.933·10-11 / 1.105·10-10 / 2.054·10-10 / 4.059·10-11 / 7.426·10-11
B2m / 1.279·10-10 / 1.832·10-10 / 5.106·10-11 / 5.571·10-11 / 2.981·10-10 / 8.232·10-10 / 5.785·10-11 / 3.007·10-10

Table 6 supplementary -Descriptive statistical analysis of the candidate genes in mouse medial septum at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 2.539·10-10 / 4.082·10-10 / 9.892·10-11 / 1.437·10-10 / 2.116·10-10 / 4.119·10-10 / 8.416·10-11 / 1.345·10-10
18 s / 1.027·10-06 / 1.640·10-06 / 4.163·10-07 / 5.212·10-07 / 7.415·10-07 / 1.534·10-06 / 2.925·10-07 / 4.663·10-07
Tfrc / 2.887·10-10 / 3.702·10-10 / 2.148·10-10 / 6.969·10-11 / 2.256·10-10 / 3.859·10-10 / 8.393·10-11 / 1.315·10-10
Gapdh / 4.071·10-09 / 6.348·10-09 / 3.072·10-09 / 1.333·10-09 / 4.605·10-09 / 1.089·10-08 / 1.292·10-10 / 4.107·10-09
Gusb / 9.212·10-11 / 1.173·10-10 / 6.170·10-11 / 2.071·10-11 / 6.829·10-11 / 1.043·10-10 / 2.729·10-11 / 3.181·10-11
B2m / 1.53·10-10 / 2.104·10-10 / 9.153·10-11 / 5.311·10-11 / 2.137·10-10 / 3.643·10-10 / 7.241·10-11 / 1.216·10-10

Table 7 supplementary -Descriptive statistical analysis of the candidate genes in mouse frontal neocortex at postnatal day 7 and at 6 months of age

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 1.982·10-10 / 2.643·10-10 / 3.643·10-11 / 9.737·10-11 / 7.404·10-10 / 1.757·10-09 / 3.710·10-10 / 5.774·10-10
18 s / 1.184·10-06 / 2.394·10-06 / 3.746·10-07 / 7.851·10-07 / 2.288·10-06 / 5.875·10-06 / 9.697·10-07 / 2.022·10-06
Tfrc / 4.840·10-10 / 7.718·10-10 / 1.236·10-10 / 2.356·10-10 / 5.404·10-10 / 1.052·10-09 / 2.266·10-10 / 3.058·10-10
Gapdh / 5.491·10-09 / 1.227·10-08 / 1.577·10-09 / 4.049·10-09 / 1.043·10-08 / 1.990·10-08 / 5.416·10-09 / 5.667·10-09
Gusb / 7.434·10-11 / 1.092·10-10 / 7.553·10-12 / 3.937·10-11 / 1.017·10-10 / 1.995·10-10 / 5.984·10-11 / 5.591·10-11
B2m / 1.315·10-10 / 2.252·10-10 / 1.898·10-11 / 7.713·10-11 / 3.863·10-10 / 8.952·10-10 / 2.081·10-10 / 2.878·10-10

Table 8 supplementary -Descriptive statistical analysis of the candidate genes in mouse olfactory bulb at P7 and at 6M

Postnatal day 7 / 6 months
Gene / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd / Avg 2 -CT / Max 2 -CT / Min 2 -CT / Sd
Pgk1 / 3.61·10-10 / 4.071·10-10 / 3.143·10-10 / 3.792·10-11 / 4.959·10-10 / 5.72510-10 / 4.501·10-10 / 5.335·10-11
18 s / 1.299·10-06 / 1.528·10-06 / 8.577·10-07 / 3.000·10-07 / 1.390·10-06 / 1.76410-06 / 1.084·10-06 / 3.143·10-07
Tfrc / 4.245·10-10 / 4.751·10-10 / 3.001·10-10 / 8.325·10-11 / 2.899·10-10 / 3.53210-10 / 2.132·10-10 / 5.825·10-11
Gapdh / 6.078·10-09 / 7.127·10-09 / 4.998·10-09 / 1.190·10-09 / 9.672·10-09 / 1.35910-08 / 7.607·10-09 / 2.690·10-09
Gusb / 8.713·10-11 / 1.184·10-10 / 7.455·10-11 / 2.091·10-11 / 7.565·10-11 / 1.12610-10 / 3.888·10-11 / 3.075·10-11
B2m / 2.686·10-10 / 3.449·10-10 / 1.982·10-10 / 6.241·10-11 / 3.467·10-10 / 4.784·10-10 / 1.200·10-10 / 1.586·10-10

Table 9a supplementary -Comparison of the six candidate reference genes in mouse hemibrain at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.245 / Pgk1 / 0.207 / Pgk1 / 0.856
2 / Pgk1 / 0.261 / Tfrc / 0.269 / Gusb / 0.893
3 / Gusb / 0.283 / Gusb / 0.310 / B2m / 1.002
4 / 18s / 0.348 / 18s / 0.390 / 18s / 1.006
5 / B2m / 0.351 / B2m / 0.441 / Gapdh / 1.052
6 / Gapdh / 0.370 / Gapdh / 0.453 / Tfrc / 2.001
Best combination of two genes / Pgk1+ Gusb / 0.141

Table 9b supplementary -Comparison of the six candidate reference genes in mouse hemibrain at 6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / B2m / 0.126 / Gusb / 0.058 / Gusb / 2.892
2 / Pgk1 / 0.138 / Pgk1 / 0.099 / Pgk1 / 2.896
3 / Gusb / 0.139 / B2m / 0.120 / B2m / 2.904
4 / Gapdh / 0.238 / 18s / 0.188 / 18s / 2.937
5 / 18s / 0.266 / Gapdh / 0.231 / Tfrc / 3.037
6 / Tfrc / 0.312 / Tfrc / 0.291 / Gapdh / 13.396
Best combination of two genes / Pgk1+ Gusb / 0.074

Table 9c supplementary -Candidate reference genes in mouse hemibrain (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gapdh / 0.449 / Pgk1 / 0.180 / Pgk1 / 0.764
2 / B2m / 0.504 / Tfrc / 0.226 / B2m / 0.878
3 / Pgk1 / 0.550 / Gusb / 0.327 / Gusb / 0.905
4 / Tfrc / 0.647 / B2m / 0.354 / Gapdh / 0.963
5 / Gusb / 0.783 / 18s / 0.442 / 18s / 1.014
6 / 18s / 0.855 / Gapdh / 0.448 / Tfrc / 1.452
Best combination of two genes / Pgk1 + Tfrc / 0.118 / Pgk1 + B2m / 0.376

Table 10a - Comparison of the six candidate reference genes in mouse cerebellum at P7.

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gapdh / 0.443 / Gusb / 0.076 / Gusb / 0.464
2 / B2m / 0.498 / Pgk1 / 0.160 / Pgk1 / 0.521
3 / Pgk1 / 0.564 / Gapdh / 0.196 / Gapdh / 0.549
4 / Tfrc / 0.594 / Tfrc / 0.399 / Tfrc / 0.720
5 / Gusb / 0.613 / B2m / 0.461 / B2m / 0.735
6 / 18s / 0.973 / 18s / 0.530 / 18s / 0.844
Best combination of two genes / Pgk1+ Gusb / 0.220

Table 10b - Comparison of the six candidate reference genes in mouse cerebellum at 6M.

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gusb / 0.331 / Pgk1 / 0.109 / B2m / 0.531
2 / Tfrc / 0.390 / Tfrc / 0.131 / Pgk1 / 0.542
3 / B2m / 0.407 / B2m / 0.139 / Tfrc / 0.553
4 / Pgk1 / 0.414 / Gusb / 0.240 / Gusb / 0.622
5 / 18s / 0.539 / 18s / 0.489 / 18s / 0.831
6 / Gapdh / 0.668 / Gapdh / 0.786 / Gapdh / 1.184
Best combination of two genes / Tfrc+ B2m / 0.173

Table 10c supplementary - Candidate reference genes and the best combination of two genes in mouse cerebellum (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / B2m / 0.512 / Pgk1 / 0.125 / Pgk1 / 0.595
2 / Gapdh / 0.526 / Tfrc / 0.170 / Tfrc / 0.693
3 / Pgk1 / 0.557 / B2m / 0.196 / B2m / 0.706
4 / Tfrc / 0.620 / 18s / 0.268 / Gusb / 0.796
5 / Gusb / 0.872 / Gapdh / 0.305 / 18s / 0.841
6 / 18s / 0.930 / Gusb / 0.342 / Gapdh / 0.937
Best combination of two genes / Pgk1+
Tfrc / 0.126 / Pgk1+
B2m / 0.335

Table 11a supplementary - Comparison of the six candidate reference genes in mouse brain stem at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.128 / Gusb / 0.132 / Pgk1 / 0.948
2 / 18s / 0.273 / Pgk1 / 0.207 / Tfrc / 1.004
3 / Gusb / 0.335 / Tfrc / 0.349 / Gusb / 1.040
4 / Pgk1 / 0.456 / 18s / 0.567 / 18s / 1.134
5 / Gapdh / 0.666 / B2m / 0.850 / B2m / 1.450
6 / B2m / 1.067 / Gapdh / 1.247 / Gapdh / 1.897
Best combination of two genes / 18s+ Tfrc / 0.374

Table 11b supplementary - Comparison of the six candidate reference genes in mouse brain stemat 6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / 18s / 0.410 / Gusb / 0.309 / 18s / 0.767
2 / Gapdh / 0.427 / 18s / 0.310 / Tfrc / 0.776
3 / Tfrc / 0.512 / Tfrc / 0.311 / Gusb / 0.857
4 / Gusb / 0.529 / Gapdh / 0.521 / Gapdh / 0.914
5 / Pgk1 / 0.719 / B2m / 0.615 / B2m / 1.092
6 / B2m / 0.794 / Pgk1 / 0.793 / Pgk1 / 1.246
Best combination of two genes / 18s+ Tfrc / 0.272

Table 11c supplementary - Candidate reference genes and the best combination of two genes in mouse brain stem (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.352 / Gusb / 0.105 / Gusb / 0.964
2 / 18s / 0.369 / Tfrc / 0.152 / Tfrc / 0.983
3 / Gusb / 0.413 / 18s / 0.199 / 18s / 1.043
4 / Gapdh / 0.519 / Pgk1 / 0.228 / Pgk1 / 1.133
5 / Pgk1 / 0.666 / B2m / 0.328 / B2m / 1.259
6 / B2m / 0.881 / Gapdh / 0.397 / Gapdh / 1.528
Best combination of two genes / Tfrc + Gusb / 0.096 / 18s + Tfrc / 0.309

Table 12a supplementary - Comparison of the six candidate reference genes in mouse hippocampus at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.105 / 18s / 0.096 / 18s / 0.467
2 / Gapdh / 0.196 / Pgk1 / 0.100 / Pgk1 / 0.496
3 / Tfrc / 0.196 / Gapdh / 0.293 / Gapdh / 0.608
4 / 18s / 0.231 / B2m / 0.316 / B2m / 0.629
5 / B2m / 0.232 / Tfrc / 0.462 / Gusb / 0.757
6 / Gusb / 0.240 / Gusb / 0.468 / Tfrc / 0.760
Best combination of two genes / Pgk1+18s / 0.278

Table 12b supplementary - Comparison of the six candidate reference genes in mouse hippocampus at 6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.108 / Pgk1 / 0.084 / Pgk1 / 0.449
2 / Tfrc / 0.201 / Tfrc / 0.249 / Gapdh / 0.561
3 / 18s / 0.226 / Gapdh / 0.273 / 18s / 0.567
4 / Gapdh / 0.278 / 18s / 0.277 / B2m / 0.571
5 / Gusb / 0.407 / B2m / 0.278 / Tfrc / 0.573
6 / B2m / 0.457 / Gusb / 0.535 / Gusb / 0.840
Best combination of two genes / 18s + B2m / 0.314

Table 12c supplementary - Candidate reference genes and the best combination of two genes in mouse hippocampus (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.196 / Pgk1 / 0.105 / Pgk1 / 0.515
2 / 18s / 0.215 / B2m / 0.181 / B2m / 0.619
3 / Tfrc / 0.274 / Tfrc / 0.207 / Gapdh / 0.637
4 / Gusb / 0.308 / Gapdh / 0.233 / Tfrc / 0.679
5 / Gapdh / 0.345 / Gusb / 0.276 / 18s / 0.698
6 / B2m / 0.387 / 18s / 0.300 / Gusb / 0.803
Best combination of two genes / Pgk1+ Tfrc / 0.117 / Pgk1+ Gapdh / 0.374

Table 13a supplementary - Comparison of the six candidate reference genes in mouse medial septum at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gusb / 0.225 / B2m / 0.089 / B2m / 0.344
2 / Tfrc / 0.241 / Tfrc / 0.157 / Tfrc / 0.382
3 / Gapdh / 0.327 / Gapdh / 0.222 / Gusb / 0.420
4 / B2m / 0.346 / Gusb / 0.226 / Gapdh / 0.430
5 / 18s / 0.508 / 18s / 0.276 / 18s / 0.502
6 / Pgk1 / 0.566 / Pgk1 / 0.369 / Pgk1 / 0.589
Best combination of two genes / Tfrc + Gusb / 0.190

Table 13b supplementary - Comparison of the six candidate reference genes in mouse medial septum at 6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gusb / 0.466 / Tfrc / 0.076 / Pgk1 / 0.825
2 / B2m / 0.569 / Gusb / 0.236 / Tfrc / 0.838
3 / Tfrc / 0.583 / Pgk1 / 0.236 / Gusb / 0.879
4 / 18s / 0.629 / 18s / 0.258 / B2m / 0.883
5 / Pgk1 / 0.636 / B2m / 0.364 / 18s / 1.017
6 / Gapdh / 0.892 / Gapdh / 1.751 / Gapdh / 2.550
Best combination of two genes / Pgk1+ B2m / 0.211

Table 13c supplementary - Candidate reference genes and the best combination of two genes in mouse medial septum (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gusb / 0.352 / Tfrc / 0.052 / Tfrc / 0.724
2 / Tfrc / 0.407 / B2m / 0.101 / Gusb / 0.751
3 / B2m / 0.512 / Gusb / 0.103 / Pgk1 / 0.772
4 / 18s / 0.554 / 18s / 0.119 / 18s / 0.808
5 / Pgk1 / 0.572 / Pgk1 / 0.135 / B2m / 0.838
6 / Gapdh / 0.667 / Gapdh / 0.441 / Gapdh / 1.750
Best combination of two genes / Tfrc + Gusb / 0.058 / Tfrc + Gusb / 0.318

Table 14asupplementary - Comparison of the six candidate reference genes in mouse frontal neocortex at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.487 / Tfrc / 0.084 / Pgk1 / 0.506
2 / Pgk1 / 0.491 / Pgk1 / 0.129 / Tfrc / 0.510
3 / Gusb / 0.530 / B2m / 0.333 / B2m / 0.616
4 / B2m / 0.586 / Gapdh / 0.358 / Gapdh / 0.635
5 / 18s / 0.663 / Gusb / 0.416 / 18s / 0.691
6 / Gapdh / 0.737 / 18s / 0.425 / Gusb / 0.701
Best combination of two genes / Gapdh+18s / 0.235

Table 14bsupplementary - Comparison of the six candidate reference genes in mouse frontal neocortex at 6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gapdh / 0.543 / Pgk1 / 0.100 / Pgk1 / 0.257
2 / Gusb / 0.550 / Gusb / 0.104 / Gusb / 0.271
3 / Tfrc / 0.566 / Gapdh / 0.140 / B2m / 0.282
4 / B2m / 0.745 / B2m / 0.142 / Gapdh / 0.290
5 / Pgk1 / 0.780 / Tfrc / 0.194 / Tfrc / 0.339
6 / 18s / 0.884 / 18s / 0.196 / 18s / 0.343
Best combination of two genes / Pgk1+ B2m / 0.107

Table 14csupplementary - Candidate reference genes and the best combination of two genes in mouse frontal neocortex (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.506 / Gapdh / 0.154 / Gapdh / 0.586
2 / Gusb / 0.543 / 18s / 0.241 / 18s / 0.632
3 / Gapdh / 0.669 / Gusb / 0.254 / B2m / 0.647
4 / 18s / 0.899 / B2m / 0.328 / Gusb / 0.649
5 / B2m / 0.926 / Pgk1 / 0.352 / Pgk1 / 0.668
6 / Pgk1 / 1.031 / Tfrc / 0.378 / Tfrc / 0.715
Best combination of two genes / Pgk1+Tfrc / 0.065 / Pgk1+ B2m / 0.239

Table 15asupplementary - Comparison of the six candidate reference genes in mouse olfactory bulb at P7

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.105 / B2m / 0.045 / B2m / 0.217
2 / Gapdh / 0.196 / Tfrc / 0.123 / Tfrc / 0.248
3 / Tfrc / 0.197 / Gapdh / 0.131 / 18s / 0.261
4 / 18s / 0.231 / Gusb / 0.145 / Gapdh / 0.270
5 / B2m / 0.232 / 18s / 0.146 / Gusb / 0.280
6 / Gusb / 0.240 / Pgk1 / 0.192 / Pgk1 / 0.325
Best combination of two genes / 18s+ Tfrc / 0.071

Table 15bsupplementary - Comparison of the six candidate reference genes in mouse olfactory bulb at6M

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.108 / Pgk1 / 0.083 / Pgk1 / 0.476
2 / Tfrc / 0.201 / Tfrc / 0.166 / 18s / 0.526
3 / 18s / 0.226 / 18s / 0.173 / Tfrc / 0.537
4 / Gapdh / 0.278 / Gapdh / 0.367 / Gapdh / 0.716
5 / Gusb / 0.407 / Gusb / 0.436 / Gusb / 0.750
6 / B2m / 0.457 / B2m / 0.633 / B2m / 0.983
Best combination of two genes / 18s+Tfrc / 0.212

Table 15csupplementary - Candidate reference genes and the best combination of two genes in mouse olfactory bulb (P7 and 6M considered together)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Pgk1 / 0.196 / 18s / 0.108 / 18s / 0.484
2 / 18s / 0.215 / Pgk1 / 0.199 / Pgk1 / 0.504
3 / Tfrc / 0.274 / B2m / 0.217 / Tfrc / 0.605
4 / Gusb / 0.308 / Gusb / 0.259 / Gusb / 0.623
5 / Gapdh / 0.345 / Gapdh / 0.285 / Gapdh / 0.635
6 / B2m / 0.387 / Tfrc / 0.290 / B2m / 0.706
Best combination of two genes / Pgk1+Tfrc / 0.122 / 18s + Tfrc / 0.379

Table 16asupplementary - Candidate reference genes in mouse brain at P7 (global analysis of the six brain regions)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.594 / Pgk1 / 0.169 / Pgk1 / 0.663
2 / Pgk1 / 0.603 / Gusb / 0.241 / Gusb / 0.761
3 / Gapdh / 0.627 / Gapdh / 0.256 / Tfrc / 0.773
4 / Gusb / 0.748 / Tfrc / 0.257 / 18s / 0.834
5 / B2m / 0.827 / B2m / 0.259 / B2m / 0.846
6 / 18s / 0.838 / 18s / 0.294 / Gapdh / 0.934
Best combination of two genes / Pgk1+ Tfrc / 0.153 / Tfrc+ 18s / 0.519

Table 16bsupplementary - Candidate reference genes in mouse brain at 6M (global analysis of the six brain regions)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Gusb / 0.55 / Tfrc / 0.099 / Tfrc / 0.744
2 / Gapdh / 0.602 / 18s / 0.133 / 18s / 0.775
3 / Tfrc / 0.605 / Pgk1 / 0.141 / Pgk1 / 0.813
4 / B2m / 0.677 / Gusb / 0.156 / Gusb / 0.837
5 / 18s / 0.701 / B2m / 0.176 / B2m / 0.854
6 / Pgk1 / 0.771 / Gapdh / 0.293 / Gapdh / 1.270
Best combination of two genes / Tfrc + 18s / 0.088 / Tfrc+ 18s / 0.519

Table 16csupplementary - Candidate reference genes in mouse brain (P7 and 6M considered together. global analysis of the six brain regions)

CV method / NormFinder method / GeNorm method
Ranking order / Gene / CV / Gene / Stability valuea / Gene / Stability value(M)b
1 / Tfrc / 0.606 / 18s / 0.167 / Pgk1 / 0.782
2 / Gapdh / 0.630 / Pgk1 / 0.173 / Tfrc / 0.800
3 / Gusb / 0.689 / Gusb / 0.178 / 18s / 0.830
4 / B2m / 0.747 / Tfrc / 0.191 / Gusb / 0.836
5 / Pgk1 / 0.748 / B2m / 0.200 / B2m / 0.886
6 / 18s / 0.78 / Gapdh / 0.213 / Gapdh / 1.131
Best combination of two genes / Pgk1+ Tfrc / 0.059 / 18s + Tfrc / 0.552

Table supplementary 17 - Pgk1 expression in mouse brain regions

E14 / P0 / P7 / P14 / P60 / 6M / 18M / Linear regression / Correlation
Hemibrain / 8.919·10-9
(±9.434·10-10) / 7.844·10-9
(±5.362·10-10) / 7.905·10-9
(±7.882 ·10-10) / 7.559·10-9
(±1.169 ·10-9) / 1.058·10-9
(±1.978 ·10-9) / 7.212·10-9
(±3.563 ·10-10) / - / y =0.851 ·10-8 +3.529 ·10-12 x / r = -0.088
P=0.617
Cerebellum / - / - / 4.043·10-10
(±2.516·10-11) / 4.053·10-10
(±3.178·10-11) / 6.01·10-10
(±6.094·10-11) / 4.150·10-10
(±5.286·10-11) / 2.948·10-10
(±1.427·10-11) / y =0.476 ·10-9 +2.984 ·10-13 x / r = -0.360
P=0.046
Brain Stem / - / - / 5.953·10-10
(±9.616·10-11) / 1.083·10-9
(±1.456·10-10 / 1.188·10-9
(±9.160·10-11) / 8.647·10-10
(±8.577·10-11) / 9.486·10-10
(±1.124·10-10) / y = 0.930·10-9 +5.415 ·10-14x / r = 0.028
P=0.088
Hippocampus / - / - / 3.165·10-10
(±4.611·10-11) / 9.044·10-10
(±9.336·10-11) / 6.835·10-10
(±1.022·10-10) / 5.365·10-10
(±8.292·10-11) / 6.375·10-10
(±1.037·10-10) / y = 0.626·10-9 +3.787 ·10-14x / r = -0.024
P=0.902
Medial septum / - / - / 4.686·10-10
(±9.680·10-11) / 5.947·10-10
(±8.210·10-11) / 4.271·10-10
(±9.123·10-11) / 3.549·10-10
(±7.474·10-11) / 5.725·10-10
(±5.921·10-11) / y = 0.472·10-9 +6.139 ·10-14x / r = 0.049
P=0.796
Frontal neocortex / - / - / 2.547·10-10
(±3.173·10-11) / 9.161·10-10
(±2.109·10-10) / 8.538·10-10
(±1.371·10-10) / 8.141·10-10
(±1.446·10-10) / 9.559·10-10
(±6.053·10-11) / y = 0.660·10-9 +6.348 ·10-13x / r = 0.282
P=0.146
Olfactory bulb / - / - / 3.541·10-10
(±3.452·10-11) / 4.877·10-10
(±1.818·10-11) / 5.871·10-10
(±1.253·10-11) / 5.063·10-10
(±2.638·10-11) / 8.251·10-10
(±4.221·10-11) / y = 0.440·10-9 +6.967 ·10-13x / r = 0.851
P=1.95·10-6

Table 1 supplementary - Housekeeping genes used for gene expression studies on mouse brain regions

Results from a PubMed search concerning the use of housekeeping genes in gene expression studies performed on different mouse brain regions. We had access to 101 articles from November 2007 to December 2006 for brain, to 70 articles from October 2007 to December 2004 for cerebellum, to 68 articles from September 2007 to December 2004 for brain stem, to 76 articles from October 2007to December 2004 for hippocampus, to 12 articles from June 2007 to May 1999 for septum, to 18 articles from December 2006 to May 1997 for neocortex and to 36 articles fromNovember 2007 to December 1994 for olfactory bulb. Each cell of the table contains the percentage of articles citing a certain reference gene, calculated on the number of all considered articles. It is notable that the whole number of the citations exceeded the number of the considered articles for each tissue, as some of them used more than one housekeeping gene.