Supplementary Figure

Supplementary Figure

Fig.1Nucleotide sequence of the mdlB. The predicted amino acid sequence for the mdlBgene is given below the DNA sequence.

1 ATGAGCCAGAATCTCTTTAACGTTGAGGACTATCGCAAGCTCGCGCAAAAGCGCTTGCCC

1 M S Q N L F N V E D Y R K L A Q K R L P

61 AAAATGGTGTACGACTATCTGGAAGGTGGGGCTGAAGACGAATACGGGGTGAAACACAAC

21 K M V Y D Y L E G G A E D E Y G V K H N

121 CGCGACGTCTTTCAGCAATGGCGATTCAAACCAAAGCGGCTGGTAGATGTCAGCCGCCGC

41 R D V F Q Q W R F K P K R L V D V S R R

181 AGTCTTCAAGCGGAAGTGCTTGGAAAGAGGCAGTCAATGCCTCTCTTGATTGGCCCAACT

61 S L Q A E V L G K R Q S M P L L I G P T

241 GGGCTGAATGGTGCGCTCTGGCCTAAAGGGGATCTCGCCCTTGCTCAAGCAGCAACCAAG

81 G L N G A L W P K G D L A L A Q A A T K

301 GCCGGCATCCCGTTCGTGCTGTCGACCGCCTCCAACATGTCCATTGAAGACCTCGCTCGT

101 A G I P F V L S T A S N M S I E D L A R

361 CAGTGTGATGGCGATCTATGGTTCCAGCTCTATGTGATCCACCGAGAGATCGCGCAGGGG

121 Q C D G D L W F Q L Y V I H R E I A Q G

421 ATGGTGCTCAAAGCCCTGCACTCCGGTTACACCACACTGGTACTCACAACAGACGTCGCA

141 M V L K A L H S G Y T T L V L T T D V A

481 GTTAATGGCTATCGCGAGCGAGACCTGCACAACCGATTCAAGATGCCGATGAGCTACACC

161 V N G Y R E R D L H N R F K M P M S Y T

541 CCAAAGGTGATGCTGGACGGATGCCTGCATCCACGCTGGTCGCTCGATCTGGTGCGCCAC

181 P K V M L D G C L H P R W S L D L V R H

601 GGCATGCCGCAACTGGCCAACTTCGTCAGCAGTCAAACGTCCAGCTTAGAGATGCAGGCA

201 G M P Q L A N F V S S Q T S S L E M Q A

661 GCATTGATGAGCCGCCAAATGGATGCCAGTTTCAACTGGGAGGCATTGAGATGGCTGCGT

221 A L M S R Q M D A S F N W E A L R W L R

721 GACCTCTGGCCGCACAAACTCCTCGTAAAGGGGTTACTCAGTGCTGAAGACGCCGATCAT

241 D L W P H K L L V K G L L S A E D A D H

781 TGCATCGCTGAAGGTGCCGACGGCGTGATCCTGTCAAACCACGGCGGTCGCCAACTCGAT

261 C I A E G A D G V I L S N H G G R Q L D

841 TGCGCGGTATCGCCAATGGAAGTTTTGGCTCAATCGGTAGCGAAAACTGGAAAACCAGTG

281 C A V S P M E V L A Q S V A K T G K P V

901 CTTATCGATAGCGGCTTCCGACGGGGTTCGGACATCGTTAAAGCGCTTGCGCTAGGTGCT

301 L I D S G F R R G S D I V K A L A L G A

961 GAGGCTGTACTCCTGGGACGTGCAACTTTGTATGGCCTTGCAGCACGAGGTGAAACGGGT

321 E A V L L G R A T L Y G L A A R G E T G

1021 GTTGACGAGGTGCTAACCCTCCTCAAAGCGGATATCGACCGCACCTTGGCCCAGATTGGA

341 V D E V L T L L K A D I D R T L A Q I G

1081 TGCCCTGACATCACCTCCCTTTCTCCTGATTACCTCCAAAGCGAGGGAGTGACTAGCACC

361 C P D I T S L S P D Y L Q S E G V T S T

1141 GCTCCAGTCGATCACCTCATTGGTAAAGGAACACACGCATAA

381 A P V D H L I G K G T H A *

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Fig.2SDS-PAGE of total proteins of E.coli/pET28a-mdlC.LaneM: protein molecular weight marker; lane1: uninduced total proteins of E.coli/pET28a-mdlC; lane2: induced total proteins of E.coli/pET28a-mdlC; lane3: uninduced total proteins of E.coli/pET28a; lane 4: induced total proteins of E.coli/pET28a

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