MS-Fit Search Results

MS-Fit Search Results

Sample ID (comment): Magic Bullet digest

Database searched: SwissProt

Molecular weight search (1000 - 100000 Da) selects 110898 entries.

Full pI range: 117326 entries

Combined molecular weight and pI searches select 110898 entries.

MS-Fit search selects 5211 entries (results displayed for top 20 matches).

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

Min. #
Peptides
to
Match / Peptide
Mass
Tolerance
(+/-) / Peptide
Masses
are / Digest
Used / Max. #
Missed
Cleavages / Cysteines
Modified by / Peptide N terminus / Peptide
C terminus / Input #
Peptide
Masses
4 / 50.000
ppm / monoisotopic / Trypsin / 1 / carbamidomethylation / Hydrogen (H) / Free Acid (OH) / 73

Result Summary

Rank / MOWSE / #(%)
Masses
Matched / Protein
MW
(Da)/pI / Species / SwissProt.
01Nov02
Accession # / Protein Name
1 / 2.13e+008 / 12/73 (16%) / 61055.0 / 5.70 / HUMAN / P10809 / 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein) (HuCHA60)
2 / 4.46e+006 / 10/73 (13%) / 60955.8 / 5.91 / MOUSE / P19226 / 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65)
3 / 4.46e+006 / 10/73 (13%) / 60988.9 / 5.84 / CRIGR / P18687 / 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1)
4 / 9.07e+004 / 8/73 (10%) / 50101.4 / 6.41 / MOUSE / P01872 / Ig mu chain C region secreted form
5 / 8.63e+004 / 8/73 (10%) / 52656.2 / 5.88 / MOUSE / P01873 / Ig mu chain C region membrane-bound form
6 / 2.97e+004 / 10/73 (13%) / 55356.6 / 5.71 / CAMJE / O52908 / Flagellar biosynthesis protein flhF (Flagella associated GTP-binding protein)
7 / 1.62e+004 / 7/73 (9%) / 63222.2 / 5.19 / METAC / Q8TUM3 / Arginyl-tRNA synthetase (Arginine—Trna ligase) (ArgRS)
8 / 1.55e+004 / 7/73 (9%) / 50544.5 / 8.85 / CAEEL / Q22555 / Nuclear hormone receptor family member nhr-44
9 / 1.15e+004 / 12/73 (16%) / 94171.9 / 5.97 / MYCTU / O53152 / Hypothetical protein Rv1461 [Contains: Endonuclease PI-MtuHIIP (Mtu pps1 intein)]
10 / 1.13e+004 / 8/73 (10%) / 97482.7 / 8.34 / ARATH / Q9T052 / Phospholipase D gamma 3 (AtPLDgamma3) (PLD gamma 3)
11 / 1.06e+004 / 6/73 (8%) / 97630.3 / 6.51 / GIALA / P51776 / Pyruvate,Phosphate Dikinase Pyruvate, Orthophosphate
Dikinase
12 / 8.4e+003 / 9/73 (12%) / 46954.6 / 7.06 / PSEFL / P52155 / Transcription termination factor RHO
13 / 8.16e+003 / 9/73 (12%) / 70973.4 / 5.32 / THEAN / P16019 / Heat Shock 70 KD protein (HSP 70.1)
14 / 7.89e+003 / 8/73 (10%) / 59518.9 / 5.13 / HUMAN / P13645 / Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
15 / 7.58e+003 / 6/73 (8%) / 34350.7 / 9.34 / YEAST / P38077 / ATP synthase gamma chain, mitochondrial precursor
16 / 7.06e+003 / 11/73 (15%) / 90571.2 / 9.43 / NEIMA / Q9JWA2 / Hemoglobin-haptoglobin utilization protein B precursor
17 / 6.24e+003 / 7/73 (9%) / 45625.6 / 4.74 / METKA / P58813 / Phosphoglycerate mutase Allene oxide synthase, chloroplast precursor
18 / 5.2e+003 / 8/73 (10%) / 59669.9 / 7.02 / LINUS / P48417 / (Hydroperoxide dehydrase) (Cytochrome P450 74A)
19 / 4.24e+003 / 7/73 (9%) / 46746.1 / 9.59 / BPPH8 / P06155 / Integrase
20 / 3.89e+003 / 7/73 (9%) / 60578.0 / 5.76 / THETN / Q8RCH9 / Urocanate hydratase (Urocanase)
(Imidazolonepropionate hydrolase)

Detailed Results

1. 12/73 matches (16%). 61055.0 Da, pI = 5.70. Acc. # P10809. HUMAN. 60 kDa heat shock protein, mitochondrial

precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein) (HuCHA60).

m/z
submitted / MH
matched / Delta
ppm / start / end / Peptide Sequence / Modifications
833.3795 / 833.3906 / -13.3367 / 302 / 309 / (K)APGFGDNR(K)
855.4486 / 855.4688 / -23.6461 / 134 / 141 / (K)GANPVEIR(R)
961.4818 / 961.4856 / -3.9462 / 302 / 310 / (K)APGFGDNRK(N)
1344.7076 / 1344.7163 / -6.4967 / 61 / 72 / (R)TVIIEQSWGSPK(V)
1428.7894 / 1428.8136 / -16.9574 / 143 / 156 / (R)GVMLAVDAVIAELK(K)
1504.7365 / 1504.7569 / -13.5358 / 206 / 218 / (K)TLNDELEIIEGMK(F)
1601.7471 / 1601.7521 / -3.1292 / 237 / 249 / (K)CEFQDAYVLLSEK(K)
1684.9232 / 1684.9056 / 10.4136 / 430 / 446 / (R)AAVEEGIVLGGGCALLR(C)
1771.8602 / 1771.8536 / 3.6937 / 447 / 462 / (R)CIPALDSLTPANEDQK(I)
1919.0678 / 1919.0714 / -1.8717 / 251 / 268 / (K)ISSIQSIVPALEIANAHR(K)
2365.3466 / 2365.3343 / 5.2218 / 269 / 290 / (R)KPLVIIAEDVDGEALSTLVLNR(L)
2560.2610 / 2560.2491 / 4.6473 / 97 / 121 / (K)LVQDVANNTNEEAGDGTTTATVLAR(S)

61 unmatched masses: 807.3361 810.4794 813.2400 817.2957 836.3026 839.1650 842.5100 855.0473 861.0666 877.0331

893.0086 903.4262 920.4649 1037.5137 1045.5553 1070.5693 1104.5591 1118.5106 1148.5499 1162.5755 1165.5900

1178.6079 1179.6085 1202.6227 1234.6648 1268.6957 1318.6238 1320.6060 1330.7564 1365.6504 1383.6660 1390.6856

1398.6730 1419.6952 1427.7834 1434.7630 1460.8052 1474.7763 1475.7630 1493.7232 1505.6980 1512.6996 1519.7151

1537.7213 1549.7095 1593.8256 1597.8040 1633.7943 1635.8417 1649.8053 1666.8754 1683.9202 1698.9428 1716.8511

1749.8699 1791.7564 1855.0160 2211.1046 2225.1132 2239.1385 2277.0358

The matched peptides cover 29% (167/573 AA's) of the protein.

Coverage Map for This Hit (MS-Digest index #): 101132

2. 10/73 matches (13%). 60955.8 Da, pI = 5.91. Acc. # P19226. MOUSE. 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65).

m/z
submitted / MH
matched / Delta
ppm / start / end / Peptide Sequence / Modifications
833.3795 / 833.3906 / -13.3367 / 302 / 309 / (K)APGFGDNR(K)
855.4486 / 855.4688 / -23.6461 / 134 / 141 / (K)GANPVEIR(R)
961.4818 / 961.4856 / -3.9462 / 302 / 310 / (K)APGFGDNRK(N)
1344.7076 / 1344.7163 / -6.4967 / 61 / 72 / (R)TVIIEQSWGSPK(V)
1428.7894 / 1428.8136 / -16.9574 / 143 / 156 / (R)GVMLAVDAVIAELK(K)
1504.7365 / 1504.7569 / -13.5358 / 206 / 218 / (K)TLNDELEIIEGMK(F)
1601.7471 / 1601.7521 / -3.1292 / 237 / 249 / (K)CEFQDAYVLLSEK(K)
1684.9232 / 1684.9056 / 10.4136 / 430 / 446 / (R)AAVEEGIVLGGGCALLR(C)
2365.3466 / 2365.3343 / 5.2218 / 269 / 290 / (R)KPLVIIAEDVDGEALSTLVLNR(L)
2560.2610 / 2560.2491 / 4.6473 / 97 / 121 / (K)LVQDVANNTNEEAGDGTTTATVLAR(S)

63 unmatched masses: 807.3361 810.4794 813.2400 817.2957 836.3026 839.1650 842.5100 855.0473 861.0666 877.0331

893.0086 903.4262 920.4649 1037.5137 1045.5553 1070.5693 1104.5591 1118.5106 1148.5499 1162.5755 1165.5900

1178.6079 1179.6085 1202.6227 1234.6648 1268.6957 1318.6238 1320.6060 1330.7564 1365.6504 1383.6660 1390.6856

1398.6730 1419.6952 1427.7834 1434.7630 1460.8052 1474.7763 1475.7630 1493.7232 1505.6980 1512.6996 1519.7151

1537.7213 1549.7095 1593.8256 1597.8040 1633.7943 1635.8417 1649.8053 1666.8754 1683.9202 1698.9428 1716.8511

1749.8699 1771.8602 1791.7564 1855.0160 1919.0678 2211.1046 2225.1132 2239.1385 2277.0358

The matched peptides cover 23% (133/573 AA's) of the protein.

Coverage Map for This Hit (MS-Digest index #):43159

3. 10/73 matches (13%). 60988.9 Da, pI = 5.84. Acc. # P18687. CRIGR. 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1).