1. Oligonucleotides for Plasmid Construction

1. Oligonucleotides for plasmid construction

pGSO146 pET21b-hfq (ampr)

#731 5’-TAT ACA TAT GGC TAA GGG GCA ATC TTT ACA AG

#680 5’-CCA AGC TTA TTC GGT TTC TTC GCT GTC C

pGSO147 pSP64-T7/oxySG102C (ampr) linearized with HindIII

#819 5’-CTT TTG CGG ATC TCC ACG ATC CGC AAG CTT GTA

#820 5’-TAC AAG CTT GCG GAT CGT GGA GAT CCG CAA AAG

pGSO136 pSP64- T7/oxyS64®109 (ampr) linearized with HindIII

#691 5'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA ATA ACT AAA GCC AA

#690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO137 pSP64- T7/spf (ampr) linearized with HindIII

#786 5'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGT AGG GTA CAG AGG TAA G

#787 5'-TAC AAG CTT TAA AAA ACG CCC CAG TC

pGSO138 pSP64- T7/fhlA-107®+96 (ampr) linearized with HindIII

#790 5’-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA CCA CAC GCC GAA CCG C

#791 5'-TAC AAG CTT CTC ACA CAG CGA GGC C

pGSO148 pSP64- T7/fhlAC-13G (ampr) linearized with HindIII

#821 5’-GCT TTG CGG TGC TTT CGT GGA AGA ACA AAA TGT

#822 5’-ACA TTT TGT TCT TCC ACG AAA GCA CCG CAA AGC

pGSO115 pCR2.1-T3/6S (ampr, kanr) linearized with SmaI

T3/6S(5'-3) 5'- GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GAT TTC TCT GAG ATG TTC GC

6S(3'-3) 5'- CGC CCG GGA ATC TCC GAG ATG CCG CC

pGSO113 pCR2.1-T3/5S (ampr, kanr) linearized with DraI

T3/5S(5'-1) 5'-GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GTG CCT GGC GGC CGT AGC GCG

5S(3'-1) 5'-CCC CGG GTT TAA ATG CCT GGC AGT TCC CTA CTC)

pGSO139 pCR2.1-T3/oxyS (ampr, kanr) linearized with HindIII

#797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO140 pCR2.1-T3/oxyS1®90 (ampr, kanr) linearized with DraI

#797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#800 5'-GCT TTA AAA GTT CAC GTT GGC TTT AG

pGSO141 pCR2.1-T3/oxyS1®69 (ampr, kanr) linearized with SspI

#797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#799 5'-GCA ATA TTA TTC GAG TTG AGA AAC AC

pGSO142 pCR2.1-T3/oxyS48®109 (ampr, kanr) linearized with HindIII

#798 5'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G

#690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO143 pCR2.1-T3/oxyS48®90 (ampr, kanr) linearized with DraI

#798 5'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G

#800 5'-GCT TTA AAA GTT CAC GTT GGC TTT AG

pGSO144 pGEM2-T3/oxyS48®69 (ampr) linearized with SspI

#801 5’-AAT TCA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC GAA TAA TAT TA

#802 5’-AGC TTA ATA TTA TTC GAG TTG AGA AAC TCT CCT TTA GTG AGG GTT AAT TG

pGSO145 pGEM2-T3/oxyS64®90 (ampr) linearized with DraI

#803 5’-AAT TCA ATT AAC CCT CAC TAA AGG AAT AAC TAA AGC CAA CGT GAA CTT TTA A

#804 5’-AGC TTT AAA AGT TCA CGT TGG CTT TAG TTA TTC CTT TAG TGA GGG TTA ATT G

2. Oligonucleotides for oligo blocking analysis

anti-OxyS1-20 5’-AAG AGG TGC CGC TCC GTT TC

anti-OxyS6-27 5’-GGG TTA AAA GAG GTG CCG CTC C

anti-OxyS20-40 5’-GCA GTG ACT TCA AGG GTT AA

anti-OxyS52-71 5’-AGT TAT TCG AGT TGA GAA AC

anti-OxyS64-83 5’-CAC GTT GGC TTT AGT TAT TC

anti-OxyS76-92 5’-GCA AAA GTT CAC GTT GG

3. Oligonucleotides for primer extension assay

OxyS RNA

#188 5’-GCA AAA GTT CAC GTT GG

fhlA mRNA

#153 5’-TGT CCG AGA TCA CTC ATC GGT GTA TAT GAC

Spot42 RNA

#823 5’-CAG CCA AAT CCG ATT ACG