Table S1. US Patent Search for Plant Biotechnology Patents from 2001 to September 15, 2011

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Table S1. US Patent search for plant biotechnology patents from 2001 to September 15, 2011, using the search terms “Transgenic plant” and “NUE”. KD = Knockdown or reduced gene expression, KO = knockout or complete loss of gene expression and OX = gene over-expression.

Patent number / Gene/ gene product / Phenotype claimed / Inventors and assignee (s)
20080222753; 20110225675 / Ammonium transporters 1, 2. / KO/KD of chloroplast and/or leaf AMT(s) to minimize NH4+ loss, lead to better N assimilation. Increased dry matter and plant yield. / Gupta et al., Sept. 2011, Pioneer Hi-Bred International, Inc.
20110217780 / glutamine phenylpyruvate transaminase (GPT), Glutamine synthetase (GS1) stacked / GPT+GS1 OX Chlorella faster growth rate, increased chlorophyll production, increased RUBISCO level, increased NUE, increased biomass yield, increased 2-oxoglutaramate levels. / Unkefer et al., Sept. 2011 Los Alamos National Security, University of Maine
20110214207 / Two ISYT (synovial sarcoma translocation) intrinsic yield genes / OX of at least two ISYT polypeptides promotes plant growth and gives plants having enhanced yield-related traits. / Frankard et al., Sept. 2011, BASF Plant Science Company
20110209245; 20110099667 / LNT6 / Plant exhibits increased N stress tolerance with LNT6-OX. / Allen et al., Sept. 2011, E.I. du Pont de Nemours and Company and Pioneer Hi-Bred International
20090119804; 20100269218; 20110039263 / LNT2 from Arabidopsis At5g50930 gene; (lnt2-2 and lnt2-3 splice variants) / Growth of LNT2-OX under low N in field conditions results in increased N stress tolerance. / Aukerman et al., 2011, E.I. du Pont de Nemours and Company and Pioneer Hi-Bred International
20110023356 / LNT5 from Arabidopsis At1g80980 and a homologue from maize AC204252.3. / OX with ubiquitin promoter, increase in yield, biomass, or both, under limiting N. Increased N stress tolerance. / Aukerman et al., 2011, E.I. Du Pont de Nemours and Company and Pioneer Hi-Bred International
20110035837 / LNT3 / OX with ubiquitin promoter, increase in yield, biomass, or both, under limiting N. Increased N stress tolerance. / Aukerman et al., 2011, E.I. du Pont de Nemours and Company and Pioneer Hi-Bred International
20110209241 / Paclobutrazol resistance (PRE-like), SUMO Conjugating 1 (SCE1), Yield Enhancing Factor 1 (YEF1), subgroup III glutaredoxin (sister of FT) / Modulating expression of PRE-like increased seed yield. Modulating expression of SCE1 enhanced yield-related traits. YEF1 increased yield. Sister of FT protein altered root:shoot ratio. / Hatzfeld et al., 2011, BASF Plant Science Gmbh
20110195843 / 3-PGDH, adenylate kin, Cyc-NPDase, cys syn, EPPase, ger-ger reductase, hor-A-factor pre, mt succ-fum transporter, methylase hemk, myo-inositol transp, oxidoreductase, PPCtrans-isomerase, PK, R-5-P isomerase, / OX, increased yield / Thimm et al., 2011, BASF Plant Science Gmbh
20110167517 / Maize diurnal cycle genes: tocA, tocB, prr73, prr37, prr59, ztlA, ztlB, GIGANTIA orthologs: gigz1A, gigz1B / OX with a diurnal regulatory element, improved plant growth and improved source-sink relationships.
Field experiments. / Danilevskaya et al., 2011, Pioneer Hi-Bred International, Inc.
20110154530 / PPCK, arg/ala aminopeptidase, D-alanyl-D-ala CP, DGP-phosphatase, dityr transporter, farnesyl-diP farnesyl transferase, NAD+-dep betaine aldehyde DH, ser hydrolase, ketoconazole resistance TF, uridine kin, yal043c-a, ybr071w, ydr445c / OX of yield-Related Proteins, increased yield / Blasing et al., 2011, BASF Plant Science Gmbh
20110145946 / 10 N use efficiency genes / OX with 35S promoter, improved plant NUE under limiting N, larger plants with a larger photosynthetic capacity under limiting. / Vinocur et al., 2011, Evogene
20110119791 / Aquaporins / OX with 35S promoter, At6669 promoter, increased fertilizer use efficiency / Ronen et al., 2011, Evogene
20110098183 / 30S rib S11, 60S rib, Adaptin medium chain homolog APM2, B0252, BRICK1-like, Cav1, Chloroplast chaperonin, DNA polymerase, flagellar protein, G2/mitotic-specific cyclin, GRE1 (Hydrophilin), Membrane protein, ORF YPL249c-a, RNA polymerase II cyclin-like subunit, Ser/thre-PK, Short chain DH, Sterol-C-methyltransferase, TF (farr), Ykr015c, YPL167C / OX, increased yield / Blasing et al., 2011, BASF Plant Science Gmbh
20110047649
20100050293 / Opine synthase and oxidase genes / Spatial/ temporal separated expression, increased opine activity and/or level results in dwarf plants. Modulation of opine activity in the plant modulates both root and shoot growth. / Gupta et al., 2011, Pioneer Hi-Bred International, Inc.
20110061132 / YNT1; yeast nitrate transporter 1, YNR1; nitrate reductase 1 (with some aa substitutions, transformed separately or stacked) / Using root promoters from: tubulin; ubiquitin, metallothionein; lipid transfer protein 2; banana streak virus truncated and full versions, maize NAS2, maintained or increased yield under low or normal N fertility. Field yield trials. / Wang and Loussaert 2011, Pioneer Hi-Bred International, Inc.
20110047647 / Four Shuffled YNT1; isolated nitrate transporter variants / Maize root metallothionein promoter, increased yield. Field yield trials. / Liu et al., 2011, Pioneer Hi-Bred International, Inc.
20110041216
20080313775 / Nitrate reductases, NR from Porphyra perforata (ppnr) and Porphyra yezoensis (pynr). Stacked transformation. / Const. promoter glyceraldehyde-3-PDH,
UBI promoter, nitrate reductase ZMNR (in maize), increased yield and growth. / Loussaert et al., 2011, Pioneer Hi-Bred International, Inc.
20110010800 / B3293-protein, and phenylacetic acid degradation operon negative regulatory protein (paax) / Targeted to sub cellular and or tissues, improved crop yield or harvestable yield / Ritte et al., 2011, BASF Plant Science Gmbh
20110010797 / Arabidopsis nitrate transporter NRT 1.7 / OX, expressed in phloem, source-to-sink remobilization of nitrate. Nitrate remobilization from older to young leaves is regulated and so plant growth is regulated. / Tsay et al. 2011, Academia Sinica
20100293665 / At1g74730-protein, At3g63270-protein, protein kinase, protein ser/thre phosphatase, SET domain protein / Inactivated or down-regulated genes, increased yield when grown under non-N-deficiency conditions. / Puzio et al., 2010, BASF Plant Science Gmbh
20100287669 / 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (inhibits ethylene synthesis) / Increase in yield, improvement of N stress tolerance through inhibition of ACC synthase can also result in increased root mass and/or length, increased ear, leaf, seed, and/or endosperm size, and/or improved standability. / Bate et al., 2010, Pioneer Hi-Bred International, Inc.
20100162433 / bZIP" TFs, some aa substitutions and alterations. / Normal phenotype. / McLaren et al., 2010
20100146663 / N assimilation/metabolism component operably linked to a plant promoter / The OX plants may be productively cultivated under low N fertilizer input or in N poor soils. Faster growing or maturing crops, higher crop yields and/or more nutritious products under ideal cultivation conditions. / Schneeberger et al., 2010, Ceres, Inc.
20100115667 / At1g67330 gene
transmembrane domain protein / Expressed in the root hair zone, lateral root, and elongation zone, novel N uptake gene in plants, increase fresh weight of plants at low N levels and to increase root and leaf mass with sucrose. / Frank and Simmons 2010, Pioneer Hi-Bred International, Inc.
20100115662 / ZmPEPC1 pro :ZmGS1-3
ZmUBI pro:ZmGS1-3
ZmRM2 pro:ZmGS1-3
pZM-PEPC::ZM-GS1-4
pZM-UBI::ZM-GS1-4
ZmRM2 PRO:ZmGS1-4 / Promoters from PEPC1, UBI, RM2 (root) Glutamine synthase sequences are provided for improving grain yield and plant growth. / Gupta and Dhugga, 2010, Pioneer Hi-Bred International, Inc.
20100107280 / Glutamine synthetase / OX and reduction, improving kernel productivity, Gln1-3 controls the number of kernels, Gln1-4 controls the size of kernels. Act on grain production, without affecting vegetative biomas. / Hirel and Perez 2010
20100037350 / Phosphoribosyl pyrophosphate synthases (PRPP synthetase, PRS) / OX, increased yield / Zank et al., 2010, BASF Plant Science Gmbh
20090300794 / NH4+ transporter and glucose-6-phosphate-DH / OX, enhanced N assimilation, accumulation and/or utilization and/or for the increased total N content / Plesch et al., 2009, Metanomics GmbH.
20090288224 / Alanine aminotransferase / OX OsANT1, root specific, Increased biomass and seed yield maintain a desired yield while reducing the need for high levels of N application. Field tested. / Good et al., 2008, Arcadia Biosciences
20090241218 / Extensin Receptor-Like Kinase, F-Box WD40, RAN-Binding Protein, Golden2-like TF, REV delta homeodomain leucine zipper, CLE protein, Seed Yield Regulator / GOS2 constitutive promoter, OX, improved growth characteristics. / Frankard et al., 2009, Cropdesign
20090199308 / Multiple genes, i.e.
Arabidopsis-Corn chimeric pyruvate orthophosphate dikinase (PPDK). / Enhanced N use efficiency, increased yield, enhanced water use efficiency, enhanced tolerance to cold stress and/or improved seed compositions. / Duff and Chitra, 2009, Monsanto Company
20090183270 / HAP3 TF / OX, increased yield / Adams et al., 2009, Monsanto Company
20090049573 / Plant HAP3 TF, N-terminal domain of a 14-3-3 protein, or both stacked / Improved tolerance to water deficit (HAP3-ox), cold or reduced N availability (N-terminal 14-3-3-ox for reduction of native 14-3-3 protein). Improved agronomic traits with stacked HAP3 TF and an N-terminal domain 14-3-3 expression / Dotson et al., 2009, Monsanto Company
20090044297 / A plant HAP3 TF protein, b) a 14-3-3 protein, or c) both / OX and reduction, increased yield / Andersen et al., 2009, Monsanto Company
20090044288 / Multiple TF proteins / Field trials with three levels of N fertilizer applied (Low (0 N), medium (80 lb/ac) and high (180 lb/ac)). Plants with enhanced NUE provide higher yield. / Abad et a., 2009, Monsanto Company
20080148432 / Seven in-absentia proteins, Pfam domain SINA; E3 ligases. / Increased yield with corn lines resistant to Mal de Rio Cuarto virus or the Puccina sorghi fungus or both / Monsanto Company
20080301839 / Sucrose phosphoylase, protein regulators / Field trials done, measured for increased N in leaf and increased yield. / Ravanello, 2008, Monsanto Company
20090025102 / Glutamate receptor genes / Reduction to altering N utilization and/or uptake in plants. / Hershey et al., 2009, Pioneer Hi-Bred International, Inc.
20080256666 / RUBISCO large subunit variant (aa substitutions and shuffling) / OX, one-fourth of total leaf N may be in Rubisco, so changes in Rubisco activity may effect NUE. / Zhu et al., 2008, Pioneer Hi-Bred International, Inc.
20090070897 / Genes from the protein domain family Pfam, RNApolL / OX by shoot specific promoters, increased yield and increased NUE. / Goldman et al., 2009, Monsanto Company
20080141390 / Genes upregulated by N in corn / OX by N inducible, const., and tissue specific promoter Corn improvement / Schnable and Dash, 2008, Iowa State University Research Foundation
20070169219
60/778,568, 60/758,8 / N responsive nucleic acids, i.e. 14-3-3 proteins, which repress nitrate reductase. / Antisense expression and OX, improvement in plant size, vegetative growth, growth rate, seedling vigor and/or biomass. N responsive nucleic acids also down-regulate genes that lead to feedback inhibition of N uptake and reduction. / Nadzan et al., 2007
20070162995 / Alanine aminotransferase / OX with OsANT1 (root), improved NUE. / Good et al., 2007, Arcadia Biosciences Ltd.
20070074312 / Flavohemoglobin (heme-binding domain and a ferredoxin reductase-like domain) / OX; decreased NO levels, increased biomass under sufficient N, increased chlorophyll content under limiting N, higher yield. Field tested. / Basra et al., 2007, Monsanto Company
20050044585 / Alanine aminotransferase / OX with btg 26, Improved NUE. / Good et al., 2005