Rauvolfia serpentina CAA44208 ------MAKLSDSQTMALFTVFLLFLSSSLALSSPILKEILIEA--
Rauvolfia verticillata AAY81922 ------MAKLSDSQTMALFTVFLLFLSSSLALSSPILKEILIEA--
Catharanthus roseus CAA71255 ------MANFSESKSMMAVFFMFFLLLLSSSSSSSSSSPILKKIFIES--
Ophiorrhiza pumilaBAB47180 ------MHSSEAMVVSILCALFLSSLSLVSSSPEFFEFIEA--
Arabidopsis thaliana LAP3 MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKH
: : : * : *. :
Rauvolfia serpentina CAA44208 ------PSYAPNSFTFDSTNKGFYTS
Rauvolfia verticillata AAY81922 ------PSYAPNSFTFDSTNKGFYTS
Catharanthus roseus CAA71255 ------PSYAPNAFTFDSTDKGFYTS
Ophiorrhiza pumilaBAB47180 ------PSYGPNAYAFDSDGE-LYAS
Arabidopsis thaliana LAP3 EVAPYKEVMGSWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTG
:.*:: *** .. *:.
Rauvolfia serpentina CAA44208 VQDGRVIKYEGPNSGFVDFAYASPYWNKAFCENSTDAEK------RPLCG
Rauvolfia verticillata AAY81922 VQDGRVIKYEGPNSGFVDFAYASPYWNKAFCENSTDAEK------RPLCG
Catharanthus roseus CAA71255 VQDGRVIKYEGPNSGFTDFAYASPFWNKAFCENSTDPEK------RPLCG
Ophiorrhiza pumilaBAB47180 VEDGRIIKYDKPSNKFLTHAVASPIWNNALCENNTNQDL------KPLCG
Arabidopsis thaliana LAP3 LADGRVVRWMGEAIGWETFSVVTSKWSEEACVRGVDSTTNKQWKHEKLCG
: ***:::: : .: .:. *.: * ...: . ***
Rauvolfia serpentina CAA44208 RTYDISYNLQNNQLYIVDCYYHLSVVGSEGGHATQLATSVDGVPFKWLYA
Rauvolfia verticillata AAY81922 RTYDISYNLQNNQLYIVDCYYHLSVVGSEGGHATQLATSVDGVPFKWLYA
Catharanthus roseus CAA71255 RTYDISYDYKNSQMYIVDGHYHLCVVGKEGGYATQLATSVQGVPFKWLYA
Ophiorrhiza pumilaBAB47180 RVYDFGFHYETQRLYIADCYFGLGFVGPDGGHAIQLATSGDGVEFKWLYA
Arabidopsis thaliana LAP3 RPLGLRFHKETGNLYIADAYYGLLVVGPEGGIATPLATHVEGKPILFAND
* .: :. :. .:**.* :: * .** :** * *** :* : :
Rauvolfia serpentina CAA44208 VTVDQRTGIVYFTDVSTLYDD--RGVQQIMDTSDKTGRLIKYDPSTKETT
Rauvolfia verticillata AAY81922 VTVDQRTGIVYFTDVSTLYDD--RGVQQIMDTSDKTGRLIKYDPSTKETT
Catharanthus roseus CAA71255 VTVDQRTGIVYFTDVSSIHDDSPEGVEEIMNTSDRTGRLMKYDPSTKETT
Ophiorrhiza pumilaBAB47180 LAIDQQAGFVYVTDVSTKYDD--RGVQDIIRINDTTGRLIKYDPSTEEVT
Arabidopsis thaliana LAP3 LDIHR-NGSIFFTDTSKRYDR--ANHFFILLEGESTGRLLRYDPPTKTTH
: :.: * ::.**.*. :* . *: .: ****::***.*: .
Rauvolfia serpentina CAA44208 LLLKELHVPGGAEVSADSSFVLVAEFLSHQIVKYWLEGPKKGTAEVLVKI
Rauvolfia verticillata AAY81922 LLLKELHVPGGAEVSADSSFVLVAEFLSHQIVKYWLEGPKKGTAEVLVKI
Catharanthus roseus CAA71255 LLLKELHVPGGAEISADGSFVVVAEFLSNRIVKYWLEGPKKGSAEFLVTI
Ophiorrhiza pumilaBAB47180 VLMKGLNIPGGTEVSKDGSFVLVGEFASHRILKYWLKGPKANTSEFLLKV
Arabidopsis thaliana LAP3 IVLEGLAFPNGIQLSKDQSFLLFTETTNCRLVKYWLEGPKMGEVEVVADL
:::: * .*.* ::* * **::. * . :::****:*** . *.: :
Rauvolfia serpentina CAA44208 P-NPGNIKRNADGHFWVSSSEELDGNMHGRVDPKGIKFDEFGNILEVIPL
Rauvolfia verticillata AAY81922 P-NPGNIKRNADGHFWVSSSEELDGNMHGRVDPKGIKFDEFGNILEVIPL
Catharanthus roseus CAA71255 P-NPGNIKRNSDGHFWVSSSEELDGGQHGRVVSRGIKFDGFGNILQVIPL
Ophiorrhiza pumilaBAB47180 R-GPGNIKRTKDGDFWVASSD----NNGITVTPRGIRFDEFGNILEVVAI
Arabidopsis thaliana LAP3 PGFPDNVRINEEGQFWVAIDCCRTPAQEVLTNNPWIRSIYFRLPIPMKLL
*.*:: . :*.***: . . *: * : : :
Rauvolfia serpentina CAA44208 PPPFAGEHFEQIQEHDGLLYIGTLFHGSVGILVYDKK---GNSFVSSH--
Rauvolfia verticillata AAY81922 PPPFAGEHFEQIQEHDGLLYIGTLFHGSVGILVYDKK---GNSFVSSH--
Catharanthus roseus CAA71255 PPPYEGEHFEQIQEHDGLLYIGSLFHSSVGILVYDDHDNKGNSYVSS---
Ophiorrhiza pumilaBAB47180 PLPYKGEHIEQVQEHDGALFVGSLFHEFVGILHNYKSSVDHHQEKNSGGL
Arabidopsis thaliana LAP3 AKTMGMRMYTVISRFDEEGKVLEVLEDRQGKVMKLWIGTVAHNHIATLPY
. . . :...* : ::. * : :. :
Rauvolfia serpentina CAA44208 ------
Rauvolfia verticillata AAY81922 ------
Catharanthus roseus CAA71255 ------
Ophiorrhiza pumilaBAB47180 NASFKEFSSF
Arabidopsis thaliana LAP3 PLTMNQ----
Supplemental figure 1: Alignment of four empirically characterized strictosidine synthases with LAP3 from Arabidopsis thaliana (Stockigt, Barleben, 2008)
Red Background: contact with indole part of strictosidine
Green background: contact with the terpenoid part of secologanin
Blue background: contact with the glucose moiety
Purple background: sequence motifs for -propeller folds
Orange background: catalytic residue (Glu309); in co1tact with indole of strictosidine
Red letters: conserved residues forming disulfide bridge that pulls 2 -helices together