Table S1: Summary of assayed constructs.
TABLE S1
Position in human chromosome 16 (kb) / Zebrafish primers / Length of the fragment / Promoter used / Restriction site used for subcloning 5’ of promoter54102 (UCRB1) / F:AGAAATGCCAACTGACCCCTGAGC
R:ACCGTCAGAAACTTGTACCAGCCC / 3316 / zIrx3a
XIrx3
/ EcoRI54129 / F: CAACCTGGACGATCAATAGC
R: GAGTCACATGGCTGTAGAGT / 2917 / zIrx3a / EcoRI
54163 / F; GGGCTTGTAAGCAATCAGTG
R: ACAACACTTGGAAGTGTAACAC / 1475 / zIrx3a / EcoRI
54272 / F: GTGATCAGATATGGCAATGAC
R: AGTTAACGGTATGATGCCGG / 1515 / zIrx3a / EcoRI
54302 / F: CTCGCTAGTGAAGTGTTCAG
R: GTGTTGTCTCTGATTGGCATG / 1754 / zIrx3a / EcoRI
54351 / F: GGTGTTGGGTGACTGAGTG
R: CTTCAGATACTGTATGCTGG / 1341 / zIrx3a / EcoRI
54355 / F: CAGTCACCAGACATGAACATGC
R: GGATGAGTCCTGTGGCGG / 1883 / zIrx3a / XbaI+Blunt(NotI)
54358 / Used that of mouse
54389 / F: CTGTCCAGCTGTCACATCG
R: AGCAGGGGCATGAACTTGG / 788 / zIrx3a / SacII+PstI
54390 / F: CCCCCTGCTTAAGACACAG
R: GCTCTCGCAGTTGTGGGC / 588 / zIrx3a
XIrx3
XOP / EcoRI
54395-97 / F: TATACTCCATGCCTCCCCC
R: TTCATTCCGCTGTGGCTGC / 1704 / zIrx3a / EcoRI
54403 / F: GAACCGCTAACTTATCCAGC
R: CCAATTGCTCATCACTGACG / 1217 / zIrx3a / EcoRI
54429 / F: TAGTCACCAGCTCACCT
R: GTAAAACTCAGGCCAGGGC / 1637 / zIrx3a / EcoRI
54444-45 / F: GATCATGTGACTTAAGACTGG
R: CTCAGCCTGTGGTAGACAC / 1798 / zIrx3a / EcoRI
54458 / F: GGCTGAACGCTCACAGGC
R: TCACCCTTCCACGGTAAGG / 939 / zIrx3a / EcoRI
54499 / F: ATGTATTCCGTATTCTGTGAGG
R: AAGACGGAGAGTGCGTGAC / 808 / zIrx3a / EcoRI
54781 / F: TCTCCTGTAGCGCAGCCG
R. TTGTTGTTTGGGAACCTGGG / 1025 / zIrx3a / EcoRI
54858 / F: AATCAAACTGTTGGTTTGATGC
R: GCAGCACACAGTGGGATGG / 548 / zIrx3a / EcoRI
55001 (UCRB2) / Used that of Fugu
55019 / F: AATGATCGCGCACTAGTGTG
R: GCCCATCCCCAAATAATAGC / 974 / zIrx3a / EcoRI
55203 / F: AATGCTGTATAGAGGCTGGC
R: CTAACAACCACTTTTCTTCCCC / 1327 / zIrx3a / EcoRI
UCRB1b / F: GCTTAAAAGAACTTGACCCCGCCC
R:GGAGGACTGCGGGGAAACTCG / 2917 / zIrx3b / EcoRI in pGL
Position in human chromosome 16 (kb) / Xenopus primers / Length of the fragment / Promoter used / Restriction site used for subcloning 5’ promoter
54039 / F: GTGACCCACCTACATTTCC
R: GTGGTAATGATTTGCCTCC / 1459 / zIrx3a / PstI+SacII
54102 (UCB1) / F: TTACTGCAGATTGAAAGGTGGGGC
R: GTTACACCAGTTCCCCCC / 971 / XIrx3a / EcoRI
54102 (UCB1) core / F: TTATTCTCAGCCATTACAAACAGGC
R: TTACTAACATGACACAGTGTGAAAAAAAAATCTC / 522 / XIrx3a / EcoRI
54129 / F: ACTTCCTGTCTGCCTTGG
R: CTTTACATACTGCGTGGGG / 1288 / XIrx3 / EcoRI
54176 / F: TAGCTTGACACTGCGGG
R: GGCAGAGGTGTAGGAGTACG / 1532 / XIrx3 / EcoRI
54182 / F: TGAGAGCAGCAGGAGGC
R: CCAAGCAGGGTGTTCAC / 1794 / XIrx3 / EcoRI
54195 / F: GCCCCTAAAAGTTGTGCC
R: AACTCTGGCCTTAGTAGGACC / 930 / XIrx3 / EcoRI
54199 / F: GGGAGAAGGGAATAAGTGC
R: GGATATGACATGACTAAGCACC / 963 / XIrx3 / EcoRI
54272 / F: CTGTGAACTTAAAATGCTTGG
R: CATTTGTTCAGGAAGCAGC / 1386 / XIrx3 / EcoRI
54302 / F: GAGAACCATGCAACATACG
R: CACCTAACCATTCACACCC / 991 / zIrx3a / PstI+SacII
54360 / F: TATGATCAAGGGGAGAAACC
R: TGATAAATTTGCCCCTAAGTG / 1199 / XIrx3 / EcoRI
54397-98 / F: TACTGAGGACAGGGAATGC
R: CCTTCTTGTTATATCATGCCC / 1291 / XIrx3 / EcoRI
54429 / F: GCCTTTCCTGTCATAGTGC
R: GTAATCCAAAGCCCCCAC / 906 / XIrx3 / EcoRI
54441 / F: GCACTCTGGAAATTGAAGG
R: TGTGTTCACTTCAATGTCTGTC / 946 / XIrx3 / EcoRI
54444 / F: CAGTGGAAGCCTCTTAGGG
R: GCTTCATGGGGGAATATG / 731 / zIrx3a / PstI+SacII
54445 / F: TCCCTTCGTGAAGTTTGTC
R: GAGAACTCCTGATAGGTCCG / 853 / XIrx3 / EcoRI
54578 / F: GGGGTCAAAACAACAGTCTG
R: CATTCTTGGTTGAGGTGGG / 792 / zIrx3a / PstI+SacII
54674 / F: CGAAAATATTGCGACGG
R: GCTGACCCTGTAGTGTTGG / 1200 / XIrx3 / EcoRI
54702 / F: GGGAAATTATGGCATCAGG
R: GGAAATGCAGGGTATAGCC / 1363 / zIrx3a / PstI+SacII
54733 / F: GCGATGCACTATTAATGAGC
R: CGGGCCTTGTGTTTGAC / 1176 / XIrx3 / EcoRI
54751 / F: TTCTATGTCTGTCAGCCTCAC
R: AATCTGCCCCTTTGTGTC / 587 / XIrx3 / EcoRI
54752 / F: TTGGGGAATTGGAATACAG
R: GAACCCAAATAAGCGCAG / 726 / XIrx3 / EcoRI
54781 / F: AACTGAACCTGCTTTGGC
R: CGTTTCATCTTCAGCCC / 870 / XIrx3 / EcoRI
54850-53 / F: AATCCCCAGAAGTCCCG
R: TTATTTGCCCAAGATCCG / 1672 / XIrx3 / EcoRI
55001 (UCB2) / F: TCCTCTCCTCTCCTGCTG
R: GAAAGGTTTTCCTGCCG / 1874 / zIrx3a / PstI+SacII
55001 (UCB2) core / F: ATTTGTAGGAGCATAGTAAATTGTTTCATCT
R: CTATTATTTGGGCCCAAGCTG / 524 / XIrx3 / EcoRI
55108 / F: CATGTTCAATCCAGTGCG
R: GCTGTCTTCCATGGGTCC / 1213 / XIrx3 / EcoRI
55203 / F: AGCCTTCCGAGTTTTCC
R: AAGTTGTAACGGCGACG / 1569 / XIrx3 / EcoRI
Control 1 between conserved regions 54129-54176 / F: CTTTTTGTGAATGAGCGGG
R: GCAATTTGTGATGCGTGC / 905 / XIrx3 / EcoRI
Control 2 between conserved regions 54578-54627 / F: CTGGGCCAGTGCACC
R: GGGAAAAGCAAGCACACAC / 755 / XIrx3 / EcoRI
Control 3 between conserved regions 55204-55256 / F: AGCACCATGCCAGACC
R: TGTAGCCACAGGTAGGAGC / 881 / XIrx3 / EcoRI
Position in human chromosome 5 (kb) / Xenopus primers / Length of the fragment / Promoter used / Restriction site used for subcloning 5’ promoter
3564 (UCRA1) / F: ATATTGGCAACAGTGGAGC
R: TGTCATTGCTTAATGTTGCTC / 2495 / XIrx3
zIrx3 / EcoRI
3564 (UCRA1) core / F: ATAGACACAGCTCAGACTATCTGGCA
R: ATGTCAAGGCTATGAAAAATGCTTAGC / 510 / XIrx3 / EcoRI
2165 (UCRA2) / F: TCTAAAATTGTGCATGACTGG
R: ATCTGTTGCCCTGGGTC / 1787 / zIrx3a / PstI+SacII
2165 (UCRA2) core / F: ATTTGTCAGGCAGTAATAAATGGC
R: ATGTCAAGGCTATGAAAAATGCTTAGC / 527 / XIrx3 / EcoRI
Position in human chromosome 16 (kb) / mouse primers / Length of the fragment (bp) / Promoter used / Restriction site used for subcloning 5’ promoter
54102 (UCRB1) / F: GCTGGAGGACAACTACAAGG
R: GCCTCTGGACCAGGGACAG / 850 / zIrx3a / EcoRI
54102 (UCRB1) core / F:CTTTCCTCCCAAGGCTCAGAG
R:AGGGGGAGAGAGAGAAAAGGA / XIrx3 / EcoRI
54390 / F: GCCTGTTCAGTGATAGTCCC
R:AAGAGCCGAAGCATCTTACC / 799 / zIrx3a / EcoRI
54358 / F: TAGCAGTACCTGGCTTGTCC
R: CCTTCCAAATGAGTTCCC / 901 / zIrx3a / EcoRI
Position in human chromosome 16 (kb) / Fugu primers / Length of the fragment / Promoter used / Restriction site used for subcloning 5’ promoter
1075 55001 (UCRB2) / F:CAGAGATCCACAGTAGCAGC
R:GTTTAGGAATCTGTGGAAGCC / 635 / zIrx3a / EcoRI
54390 / F: GGGAAATAATGCCGGTGGC
R: GGCTTCCAAACCCTGAACC / 763 / zIrx3a / EcoRI
4