Infectious hepodermal and haematopoietic necrosis
OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Infectious hypodermaland haematopoietic necrosis
Address of laboratory: / Maricultural Organism Diseases Control &
Molecular Pathology Laboratory
Yellow Sea Fisheries Research Institute,
Chinese Academy of Fishery Sciences
106 Nanjing Road
Qingdao, SD 266071
THE PEOPLE’S REPUBLIC OF CHINA
Tel.: / Phone: +86-532-5823062
Mobile: +86-138-05421513
Fax: / +86-532-5811514
e-mail address: /
website: / www.ysfri.ac.cn
Name (including Title and Position) of Head of Laboratory (Responsible Official): / Dr Jie Huang, Senior Researcher
Name(including Title and Position) of OIE Reference Expert: / Dr Jie Huang, Senior Researcher
Name (including Title and Position) of writer of this report
(if different from above): / Ms Bing Yang, Associate Researcher
Dr Jie Huang, Senior Researcher
Part I: Summary of general activities related to the disease
1. Test(s) in use/or available for the specified disease/topic at your laboratory
Maricultural Organism Diseases Control and Molecular Pathology Laboratory (MODCAMPL) of Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (YSFRI, CAFS) provided diagnosis and surveillance services for shrimp viral diseases using the recommended methods outlined the Manual of Diagnostic Tests for Aquatic Animals (OIE 2011) in 2011, as well as has been engaged in the development of new technology for the diagnosis, surveillance and prevention for shrimp viral diseases. Tests in use for infectious hypodermal and hematopoietic necrosis included polymerase chain reaction (PCR), histopathology and loop-mediated isothermal amplification (LAMP).
Test / For / Specificity / TotalPCR / Diagnosis and surveillance / All known IHHNV genotypes / 51
Histopathology / Initial diagnosis / Lesions / 6
LAMP / Confirmatory diagnosis / All known IHHNV genotypes / 225
2. Production and distribution of diagnostic reagents
MODCAMPL of YSFRI has made a series of field-usinghigh sensitive detection kits for detection of white spot syndrome virus (WSSV), infectious hepodemal and haematopoitec necrosis virus (IHHNV), Taura syndrome virus (TSV), yellow head virus (YHV), hepatopancreas parvovirus (HPV), spherical baculovirus (MBV), infectious myonecrosis virus (IMNV), tetrahedral baculovirus (BP), Penaeus vannamei nodavirus (PvNV), Macrobrachium rosenbergiinodavirus (MrNV), necrotising hepatopancreatitis bacteria (NHPB), Spiroplasma penaei. The kits were provided for the shrimp farms, hatcheries, aquaculture disease control stations, some research laboratoriesin the coastal provinces of China in 2011.The total of 26 field-using high sensitive detection kits for IHHNV was distributed.
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines
Dr Jie Huang and other staff were invited in the national consultation meeting to review the Manual of Diagnostic Tests for Aquatic Animals (OIE 2011) and provided comments and revisions for 4 locations in the IHHN chapter. The comments was submitted to OIE by the OIE Delegate of China.
4. Preparation and supply of international reference standards for diagnostic tests or vaccines
Date / Contact person,Institution, Country / Type of material provided / Quantity of material / Purpose
6/8/11 / Dr Wen-Hong, Fang
ECSFRI, CAFS, China / IHHNV DNA / 2 tubes / Positive infected shrimp tissues
7/25/11 / Dr Dong-Chun, Yan
Ludong University, China / IHHNV-infected tissues / 30g / Positive infected shrimp tissues
5. Research and development of new procedures for diagnosis and control
LAMP diagnostic method and a relavant field using kit for IHHN was developed in MODCAMPL in recent years. The specificity and sensitivity have been improved and reevaluated in 2011. Moreover, three technologies for kit shipment and preservation under normal temperature was developed in 2011. A uniform and easy sample preservation technology under normal temperature for DNA, RNA, and protein in aquatic animal tissue has been developed and evaluated for diagnostic and surveillance purposes.
6. Collection, analysis and dissemination of epizootiological data relevant to international disease control
Dr Jie Huang was noticed with the epizootiological information for massive shrimp disease outbreak in Vietnam by Dr Eduardo Leano from NACA in 2011 and rapid disseminated this information to the OIE focal point of China and the Fisheries Bureau of Ministry of Agriculture.
7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned
Dr Jie Huang and other experts in MODCAMPL were invited to participate in the meetings for the administration regulation for the international reference laboratories in China and the agreements for facilitating of rapid international materials and reagents shipment organized by the Veterinary Authority of China in 2011.
The laboratory set up two specific rooms with biosafety separation for the disease diagnostic tests and the kit production. In order to standardize and enhance the quality assurance management of the laboratory, four staff of the laboratory was assigned to take the training course for inner examination and verification in this year.
8. Provision of consultant expertise to OIE or to OIE Member Countries
Mr. Huang, a manager of a diagnostic kit manufacturer in Chinese Taipei visit our laboratory and present their new products and asked for technical advicesand opinion on the applicability for their equipment on Sept. 21, 2011. Dr Jie Huang and two more staff in the laboratory receive the visiting and provide a series of opinions for their products.
Dr Jie Huang was invited by the diagnostic kits assessment officer Dr Dr François Diaz in OIE to provide the consultatant expertise for the request from a kit manufacturer in the framework of the OIE Procedure for validation and certification of diagnostic assays Dr Jie Huang provided the encouraging suggesting and technical opinions based on the knowledge to the new equipment and kits developed by the manufacturer on Nov. 29, 2011 via email.
As an OIE expert for IHHN and an AAC member, Dr Jie Huang was invited to review Dr Lightner’s manuscript for the 2nd Globle OIE Conference on Aquatic Animal Health. Dr Jie Huang finished the revision and provided the expertise for the manuscript on Sept. 22, 2011.
9. Provision of scientific and technical training to personnel from other OIE Member Countries
None in 2011.
10. Provision of diagnostic testing facilities to other OIE Member Countries
Dr Megahed Mohamed, a PhD student at Suez Canal University, Egypt emailed Dr Jie Huang for the help for diagnostic test materials and technical training for the Mediterranean coast in Egypt. Dr Jie Huang answered the email immediately and indicate that the lab can provide specific field-using high sensitive detection kits for shrimp pathogens, histopathology services, and some technological training on disease diagnosis.The PhD student provide his personal CV and required materials listing and phone calls to Dr Huang. According to his requirement, our laboratory sent the kits samples to FedEx in Beijing for the safety identification for international shipment. The Identification and Classification Reports for Air Transport of Goods issued by the Beijing DGM Air Transport Technology Co. Ltd Transport Hazard Inspection Laboratory were just received. We are preparing to air-mail the kits to him.
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
The laboratory organised an opening ceremony for the designation of OIE Reference Laboratories for WSD and IHHN in Aug. 2011. The President of the Aquatic Animal Health Standards Commission of the OIE, the OIE Delegate of China, and the officials from the Veternary Authority, the Fisheries Bureau of Ministry of Agriculture, the General Administration of Quality Supervision, Inspection and Quarantine, and other governmental and local organisations attended the ceremony. A workshop on the knowledge of OIE was organised after the ceremony. Dr Barry Hill, the President of AAC presented the presentationsof ‘the OIE Aquatic Animal Health Standards’ and ‘OIE Reference Laboratories’. Dr Jie Huang, as a member of AAC, presented the presentation of ‘Approaches for the application of OIE standards for aquatic animal health in Asia’..
12. Participation in international scientific collaborative studies
Dr Qing-Gang Xue, a scientist in Department of Veterinary Science, Louisiana State University, USA was invited to visit the laboratory as a visiting scholar for one month collaboration on aquatic animal health during July – Aug. 2011. The interaction between WSSV and shrimp cellular receptor was investigated and concluded during his visiting.
13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)
¡ Presentations at international conferences and meetings
Huang, J., The Aquatic Animals Commission and the OIE Aquatic Code. OIE Workshop for Chinese Delegation. Feb. 2011, OIE Headquarter, Paris, France.
Huang, J., Approaches for the application and development of OIE standards for aquatic animal health in eastern Asia. OIE Training Workshop for Focal Points in Asian-Pacific, April, 2011, Hu Chi Ming City, Vietnam.
AAC, OIE.Outcomes from OIE General Session (May 2011) and AAC meeting (October 2011) (presented by Dr Barry Hill, prepared by Dr Jie Huang). NACA AGM10, Nov., 2011, Mangalore, India.
AAC, OIE. Chapter 1.3.Diseases Listed by the OIE.(Due to Indian visa, the presentation was presented by Dr Barry Hill). NACA AGM10, Nov., 2011, Mangalore, India.
Zhang,Q.-L., Huang, J.*, Li, M.-X., Du, Y.-B., Wang, Q.-T., Yang, B., Song, X.-L.Establishment of the technological platform for the development of rapid detection kits of aquatic animal pathogens. The 8th International Symposium on Diseases in Asian Aquaculture, Nov. 2011, Mangalore, India. (Due to Indian visa problem, the presentation was not presented in the conference).
¡ Scientific publications in peer-reviewed journals
Huang J, Yang B, Zhu Z-W, Song X-L, Sun X-M, Zhao H-P, Liu L. Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV). Standard of People’s Republic of China, 2011, Standardization Administration of the People’s Republic of China Press, GB/T 25878-2010.
¡ Other communications
Answering and providing materials for OIE requests for the short introduction for OIE Bulletin on the OIE RLs for WSD and IHHN, recommendation of experts and their contacting address to review some diseases chapters of Aquatic Manual, the photos for the OIE Aquatic Code Cover,
Prepareing the presentation ‘Guidelines for the OIE-NACA Regional Core’ for NACA AGM10, according to the document made byMs. Simona Forcella. the OIE Animal Health Information Department.
Participating in the national consultation meeting organised by the Chinese Veternary Authority and providing the comments for OIE Aquatic Manual and OIE Aquatic Code.
Participating in the International Scientific Program Committee of DAA8 and proposing tentative keynote and invited presentations.
Receiving the short visiting of a delegation of scientists from Belgium and Norway on aquaculture technology.
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Annual reports of OIE Reference Centres, 2011 5