Name: ______
Hour:______
Energy and Enzyme WebQuest
Objectives:
Energy:
Use the activities on topic 1 and 2 on the following site to answer questions 1-4:
http://ats.doit.wisc.edu/biology/cb/td/td.htm
1. What are the laws of Thermodynamics?
a. 1st Law:
b. 2nd Law:
2. Why do cells need energy?
3. Compare and contrast endergonic and exergonic reactions.
4. Look at the following graphs. Label each endergonic or exergonic.
ATP:
Go to the following link and use pages 2 & 3. Answer questions 5-7:
http://legacy.hopkinsville.kctcs.edu/instructors/Jason-Arnold/VLI/Module%202/m2cellfunctionandenergetics/m2cellfunctionandenergetics2.html
5. Label where energy is stored in the following drawing of ATP:
6. Explain in your own words how ATP is produced and recycled.
7. Explain what a coupled reaction is in your own words.
Enzymes:
Use the following site to answer questions 8-9.
http://www.emc.maricopa.edu/faculty/farabee/BIOBK/biobookenzym.html
Scroll down to “Enzymes: Organic Catalysts”
8. Define activation energy.
9. On the following graph that shows the energy progression of a reaction, answer the following:
a. Is this reaction endergonic or exergonic?
b. Draw a line onto the graph that represents how the graph would change in the presence of an enzyme.
Now go to the following site and choose: “The Basics" to answer questions 10-15.
http://www.northland.cc.mn.us/biology/Biology1111/animations/enzyme.html
10. What category of macromolecules do enzymes belong to?
11. They are catalysts. What does that mean?
12. Define.
a. Active Site:
b. Substrate:
13. In your own words, describe induced fit.
14. Imagine you have just had a glass of milk. Your task is to use the animation to help you draw a series of pictures that shows the catalytic cycle that shows the breakdown of lactose. Be sure to label and color code the parts involved (substrate, enzyme, products, active site, enzyme-substrate complex) and show the chronological progression with arrows.
15. What is the name of the enzyme that is lacking or not functioning correctly in individuals with lactose intolerance?
Use the following website the answer questions 16-17.
http://www.learnerstv.com/animation/animation.php?ani=324&cat=Biology
16. Explain why cells have thousands of different enzymes.
17. Explain how enzymes can make large polymers like starch.
Use the following website to answer questions 18-22.
http://leavingbio.net/ENZYMES.htm
18. Scroll to “Names of Enzymes.” What patterns do you see?
Scroll to “Factors that Effect Enzyme Activity” for questions 19-23.
19. Draw a graph to show the effect of temperature on enzyme function. Put temperature on the x-axis and rate of catalyzed reaction on the y-axis. Then describe in words why the graph appears the shape that it does.
20. Draw a graph to show the effect of pH on enzyme function. (Use the graph of chymotrypsin’s function as your example.) Put pH on the x-axis and rate of catalyzed reaction on the y-axis. Then describe in words why the graph appears the shape that it does.
21. Draw a graph to show the effect of enzyme concentration on enzyme function. Put enzyme concentration on the x-axis and rate of catalyzed reaction on the y-axis. Then describe in words why the graph appears the shape that it does. (Be careful the graph on the website is labeled incorrectly, but the shape is okay)
22. We often talk about the nucleus controlling cell activity. The control in the nucleus involves controlling gene activity (which proteins are being made and how many copies of that protein are made.) Explain the connection between controlling gene activity and controlling cell activity. Hint: Think about the graph you just drew in #21.
23. Draw a graph to show the effect of substrate concentration on enzyme function. Put substrate concentration on the x-axis and rate of catalyzed reaction on the y-axis. Then describe in words why the graph appears the shape that it does.
Inhibition:
Use the following website to answer questions 24-25.
http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html
24. What is a competitive inhibitor and how does it work?
25. What is a non-competitive inhibitor and how does it work?
Watch the 2 animations on the following website and answer questions 26-27.
http://highered.mcgraw-hill.com/sites/0072437316/student_view0/chapter8/animations.html#
26. In your own words, what is a biological pathway?
27. Describe feedback inhibition.
Use the following website to answer questions 28-29.
http://alevelnotes.com/Enzyme-Inhibitors/148
Scroll to the sections titled: Enzyme Inhibitors as Metabolic Poisons and Enzyme Inhibitors as Medicine
28. Choose one example of poison. Name it and explain how it exhibits inhibition.
29. Choose one example of a medicine. Name it and explain how it exhibits inhibition.
Application:
Go to: http://www.kscience.co.uk/animations/model.swf
First change your container size to 200. Use this size for all of the following parts.
30. Use the default settings as a control. Press setup and start to begin the reaction. Record the time it takes for all the substrate to be converted into product. (You will need to use your watch or a stopwatch to time it.) Record the time: ______
31. Choose either the number of enzymes (if your last name begins with a-m) or the number of substrates (if your last name begins with n-z) to alter. Create a data table to record your results. Try at least 5 different variations and record the times for each.
What trend does your data show?
32. Return to defaults by refreshing the webpage. Choose either the temperature (if your last name begins with a-m) or pH (if your last name begins with n-z) to alter. Create a data table to record your results. Try at least 5 different variations and record the times for each.
What trend does your data show?
33. Return to defaults by refreshing the webpage. Alter the number of inhibitors. Create a data table to record your results. Try at least 5 different variations and record the times for each.
What trend does your data show?
34. What type of inhibitor is this model demonstrating? Justify your answer.