OIE RL for Foot and Mouth Disease Dr Jef Hammond United Kingdom

OIE RL for « Foot and Mouth Disease » – « Dr Jef Hammond » – « United Kingdom »

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / FMD
Address of laboratory / The Pirbright Institute
Ash Road, Pirbright, Surrey GU24 0NF
United Kingdom
Tel.: / +44 1483 231211
Fax: / +44 1483 232621
e-mail address: /
website: / http://www.pirbright.ac.uk
Name (including Title) of Head of Laboratory (Responsible Official): / Director: Professor John K Fazakerley
Name (including Title and Position) of OIE Reference Expert: / Dr Jef Hammond- Head Reference Laboratories for FMD and SVD
Date of submission to the OIE / 25-01-13

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/

Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):

ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test / For / Specificity / Total
Cell culture / Virus isolation / Group / 685
RT-PCR / Virus genome detection / Group / 2872
ELISA / Antigen / Serotype / 900
VP1 sequencing / Phylogeny / Strain / 380

Serology

/ Vaccine Matching / ’r’ values / 1192

VNT

/ Antibody / Serotype / 3435

ELISA

/ Structural Protein Antibody / Serotype / 4383

ELISA

/ Non-structural protein Antibody / Non-serotype / 1433

ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?

Yes

3. Did your laboratory supply standard reference reagents to OIE Member Countries?

Yes

Annual reports of OIE Reference Laboratories, 2012 19

OIE RL for « Foot and Mouth Disease » – « Dr Jef Hammond » – « United Kingdom »

Type of reagent available

/ Related diagnostic test / Produced/
stored / Supplied nationally / Supplied to EU Member States / Supplied to Other Countries / Name of recipient OIE Member Countries and of institutions

Rabbit and Guinea Pig Antisera

/ Ag and Ab ELISA / Produced / None / 13ml / 141ml / Canada / NCFAD, Winnipeg
Israel / Kimron Veterinary Institute
Kenya / SAHSP, Nairobi
Iraq / Vet. Central Lab, Bagdad
Tunisia / Institut Pasteur de Tunis
Turkey / SAP Institute, Ankara
Vietnam / National Centre Vet. Diag., Ha Noi
Uganda / NADDEC, Entebe

FMD Reference sera

/ Ag and Ab ELISA / Produced / None / 24ml / 37ml / Iraq / Vet. Central Lab, Bagdad
Romania / Inst. Diagn. Animal Health
South Korea / Animal Plant & Fish. Q&I Agency, Gyeonggi Province
Vietnam / National Centre Vet. Diag.

Inactivated virus

/ Ag and Ab ELISA / Produced / None / 70ml / 87ml / Iraq / Vet. Central Lab, Bagdad
Kenya / SAHSP, Nairobi
Romania / Inst. Diagn. Animal Health
Turkey / SAP Institute, Ankara
Vietnam / National Centre Vet. Diag.

Live virus

/ VNT / Produced / 38ml / 30ml / 50ml / The Netherlands- Central Veterinary Institute
Merial-UK
Intervet/MSD- Germany

Antigen ELISA kits

/ Ag ELISA / Produced / None / 2 / 20 / Bulgaria / Bulgarian Food Safety Agency
Croatia / Serology Lab. Viral disease, Zagreb
Egypt / Animal Health Research Institute, Giza
Estonia / Estonian Veterinary & Food Lab, Tartu
Ethiopia / Nat. Animal Health Diagnostic & Investigation Centre, Sebeta
HongKong / Agricult. Fish. & Conserv. Dept.,HKSAR
New Zealand / Animal Health Laboratory, Upper Hutt
Oman / Vet. Research Laboratory, Muscat
South Korea / Animal Plant & Fish. Q&I Agency, Gyeonggi Province
UAE / Dept. of President’s Affairs, Abu Dhabi
Uganda / NADDEC, Entebe
Japan / Animal Quarantine Service, Kanagawa
Kazakhstan / Research Institute for Biol. Safety
Mongolia / State Central Veterinary Laboratory
Kuwait / Central Vet. Lab. & Animal Research

Antibody ELISA kits

/ Ab ELISA / Produced / None / 3 / 82 / Algeria / Inst. National de la Med. Veterinaire
Bangladesh / Livestock Research Institute, Dhaka
Belarus / Belarusian State Vet. Centre, Minsk
Bulgaria / Bulgarian Food Safety Agency
Croatia / Serology Lab. Viral disease, Zagreb
Estonia / Estonian Veterinary & Food Lab, Tartu
Indonesia / Veterinary Biological Centre, Surabaya
Iraq / Veterinary Central Laboratory, Bagdad
Japan / Animal Quarantine Service, Kanagawa
Kazakhstan / Research Institute for Biol. Safety
Kenya / SAHSP, Nairobi
Kuwait / Central Vet. Lab. & Animal Research
Kyrgyzstan / State Veterinary Laboratory, Bishkek
LAO PD / Dept. of Livestock & Fisheries, Vientiane
Malaysia / Dept. Veterinary Services, Putrajaya
Mongolia / State Central Veterinary Laboratory
Mozambique / Regional Animal Health Dept., Kan Tho
Pakistan / Animal Sciences Institute, Islamabad
Russia / Federal Centre Animal Health, Vladimir
Rwanda / National Veterinary Laboratory, Rubirizi
South Korea / Animal Plant & Fish. Q&I Agency, Gyeonggi Province
Sudan / Institute Endemic Diseases, Khartoum
Turkey / SAP Institute, Ankara
Vietnam / National Centre Vet. Diag.
Zambia / Central Vet. Res. Inst., Lusaka

Annual reports of OIE Reference Laboratories, 2012 19

OIE RL for « Foot and Mouth Disease » – « Dr Jef Hammond » – « United Kingdom »

4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

No

5. Did your laboratory produce vaccines?

No

6. Did your laboratory supply vaccines to OIE Member Countries?

No

ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

No

8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

No

ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes

In total the Pirbright Institute reference laboratory for FMD received 764 samples from 27 countries in 2012, 509 were collected in 2012, 230 were collected in 2011, 21 were collected in 2010, 2 were collected in 2009 and 2 were collected in 2008.

Name of OIE Member Country seeking assistance / Date of collection
(dd/mm) / No. samples received for provision of diagnosis and characterisation of FMD
AFGHANISTAN / 07-Apr-11
07-Apr-11
08-Apr-11
17-Apr-11
09-May-11
28-May-11
29-May-11
29-May-11
30-May-11
30-May-11
30-May-11
30-May-11
30-May-11
30-May-11
30-May-11
31-May-11
31-May-11
31-May-11
14-Jun-11
16-Aug-11 / 20
BAHRAIN / 05-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
06-Mar-12
06-Mar-12
13-Mar-12
20-Mar-12
21-Mar-12
21-Mar-12
21-Mar-12
26-Mar-12
05-Mar-12
05-Mar-12
05-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
28-Mar-12
05-Apr-12
08-Apr-12
18-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
22-Apr-12
24-Apr-12
24-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
25-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
26-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
29-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
30-Apr-12
07-May-12
07-May-12
07-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
08-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
09-May-12
24-May-12 / 129
BHUTAN / 26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12
26-Jun-12 / 9
BOTSWANA / 21
EGYPT / 14-Oct-10
15-Oct-10
15-Oct-10
06-Jan-11
09-Jan-11
13-Feb-11
21-Feb-11
11-Apr-11
21-Apr-11
21-Apr-11
19-May-11
14-Jun-11
13-Jul-11
08-Feb-12
17-Feb-12
18-Feb-12
27-Feb-12
27-Feb-12
27-Feb-12
28-Feb-12
29-Feb-12
29-Feb-12
29-Feb-12
29-Feb-12
29-Feb-12
01-Mar-12
03-Mar-12
04-Mar-12
08-Mar-12
08-Mar-12
09-Mar-12
28-Feb-12
09-Mar-12
13-Mar-12
13-Mar-12
22-Mar-12
02-Apr-12
03-Apr-12
25-Apr-12
08-May-12
17-May-12
19-May-12
19-May-12
21-May-12
22-May-12
22-May-12
03-Jun-12
04-Jul-12
05-Aug-12 / 49
ERITREA / 16-Nov-11
17-Nov-11
17-Nov-11
17-Nov-11
27-Nov-11
27-Nov-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11
05-Dec-11 / 18
ETHIOPIA / 15-Jul-11
15-Jul-11
29-Jul-11
29-Jul-11
29-Jul-11
29-Jul-11
19-Sep-11
19-Sep-11
19-Sep-11
04-Oct-11
04-Oct-11
08-Oct-11
08-Oct-11
08-Oct-11
08-Oct-11
08-Oct-11
08-Oct-11
08-Oct-11
14-Oct-11
14-Oct-11
14-Oct-11
14-Oct-11
14-Oct-11
25-Nov-11
25-Nov-11
25-Nov-11
25-Nov-11
29-Nov-11
29-Nov-11
29-Nov-11
29-Nov-11
29-Nov-11
01-Dec-11
01-Dec-11
01-Dec-11
01-Dec-11
14-Jan-2012
14-Jan-2012
03-Feb-2012
09-Feb-2012
09-Feb-2012
09-Feb-2012
17-Feb-2012
17-Feb-2012
20-Mar-2012
20-Mar-2012
20-May-2012
20-May-2012
29-May-2012
20-Jun-2012
20-Jun-2012 / 51
HONG KONG, SAR OF PRC / 28-Mar-12
28-Mar-12
28-Mar-12 / 3
IRAN / 19-Sep-11
08-Oct-11
13-Oct-11
15-Oct-11
29-Oct-11
31-Oct-11
08-Nov-11
09-Nov-11
12-Nov-11
13-Nov-11
18-Nov-11
18-Nov-11
19-Nov-11
20-Nov-11
21-Nov-11
21-Nov-11
23-Nov-11
28-Nov-11
11-Dec-11
25-Dec-11
25-Dec-11
26-Dec-11
27-Dec-11
27-Dec-11
31-Dec-11
01-Jan-12
02-Jan-12
03-Jan-12
03-Jan-12
03-Jan-12
10-Jan-12
12-Jan-12
12-Jan-12
14-Jan-12
17-Jan-12
26-Jan-12
26-Jan-12
17-Jun-2012
23-Jun-2012
24-Jun-2012
24-Jun-2012
26-Jun-2012
28-Jun-2012
30-Jun-2012
30-Jun-2012
02-Jul-2012
09-Jul-2012
14-Jul-2012 / 48
ISRAEL / 20-Mar-12
21-Mar-12 / 2
JAPAN / 17-Apr-10 / 1
KENYA / 17-Jan-11
30-Jan-11
02-Feb-11
15-Feb-11
19-Feb-11
19-Feb-11
01-Mar-11
01-Mar-11
13-Mar-11
Apr-11
06-Jul-11
05-Sep-11
05-Sep-11
18-Sep-11
22-Sep-11
23-Sep-11
23-Sep-11
23-Sep-11
27-Oct-11
27-Oct-11
Mar-2011
Mar-2011
Mar-2011 / 23
KUWAIT / 20-Dec-11
20-Dec-11
20-Dec-11
20-Dec-11
20-Dec-11
20-Dec-11
27-Dec-11
27-Dec-11
27-Dec-11
07-Jan-12
09-Feb-12
09-Feb-12 / 12
LIBYA / 18-Dec-11
20-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
21-Dec-11
24-Dec-11
24-Dec-11
25-Dec-11
25-Dec-11
25-Dec-11
25-Dec-11
27-Dec-11
29-Dec-11
30-Dec-11
30-Dec-11
30-Dec-11
01-Jan-12
01-Jan-12
01-Jan-12
01-Jan-12
12-Jan-12
12-Jan-12
17-Jan-12
17-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
18-Jan-12
15-Feb-12
15-Feb-12
15-Feb-12
15-Feb-12
15-Feb-12
15-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
16-Feb-12
17-Feb-12
17-Feb-12
17-Feb-12
17-Feb-12
17-Feb-12
17-Feb-12
18-Feb-12
18-Feb-12
18-Feb-12
18-Feb-12
18-Feb-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
19-Apr-12
22-Apr-12
22-Apr-12
23-Apr-12
23-Apr-12
23-Apr-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
02-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12
03-May-12 / 125
MALAYSIA / 31-Jan-12
31-Jan-12
31-Jan-12
05-Feb-12
21-Feb-12
28-Mar-12
02-Apr-12
03-Apr-12
03-Apr-12
12-Apr-12
13-Apr-12
13-Apr-12
17-Apr-12
17-Apr-12
18-Apr-12
23-Apr-12
28-Apr-12
30-Apr-12 / 18
PAKISTAN / 23-Dec-11
23-Dec-11
08-Jan-12
08-Jan-12
08-Jan-12
14-Jan-12
14-Jan-12
16-Jan-12
16-Jan-12
16-Jan-12
16-Jan-12
07-Feb-12
09-Feb-12
21-Feb-12
24-Feb-12
20-Mar-12
20-Mar-12
06-Apr-12
07-Apr-12
08-Apr-12
08-Apr-12
11-Apr-12
12-Apr-12
26-Apr-12
27-Apr-12
27-Apr-12
27-Apr-12
27-Apr-12
08-May-12
09-May-12
14-May-12
14-May-12
30-May-12
20-Jun-12
20-Jun-12
21-Jun-12
21-Jun-12
30-Jun-12
30-Jun-12
28-Sep-12
05-Nov-12
23-Nov-12
23-Nov-12 / 43
PALESTINIAN AUTONOMOUS TERRITORIES / 16-Apr-12
16-Apr-12 / 2
KINGDOM OF SAUDI ARABIA / 02-Jul-2012
07-Jul-2012
07-Jul-2012
07-Jul-2012 / 4
SRI LANKA / 09-Sep-09
13-Jan-10
13-Jan-10
18-Jan-10
15-Feb-10
31-Mar-10
31-Mar-10
21-Apr-10
21-Apr-10
02-Jun-10
04-Jun-10
09-Jun-10
02-Dec-10
03-Jun-11
14-Mar-12
15-Jun-12 / 16
SUDAN / 13-Dec-2009
11-Jan-2010
12-Jan-2010
09-Feb-2010
09-Feb-2010
09-Feb-2010
28-Feb-2011
28-Feb-2011
28-Feb-2011
01-Mar-2011
01-Mar-2011
01-Mar-2011
01-Mar-2011
03-Mar-2011
03-Mar-2011
03-Mar-2011
03-Mar-2011
03-Mar-2011
03-Mar-2011
14-Mar-2011
14-Mar-2011
02-May-2011
02-May-2011
02-May-2011
24-Nov-2011 / 25
TAIWAN, PROVINCE OF CHINA / 30-Jan-12 / 1
TANZANIA / 20-Aug-11
20-Aug-11
27-Nov-11
27-Nov-11
27-Nov-11
08-Feb-11
08-Feb-11
03-Feb-12
03-Feb-12
04-Feb-12
04-Feb-12
29-Feb-12
29-Feb-12
01-Mar-12
01-Mar-12
02-Mar-12
02-Mar-12
25-Mar-12
25-Mar-12
17-Apr-12
17-Apr-12
18-Apr-12
18-Apr-12
18-Apr-12
28-Apr-12
28-Apr-12
29-Apr-12
29-Apr-12
30-Apr-12
30-Apr-12
01-May-12
01-May-12
02-May-12
02-May-12
03-May-12
03-May-12
04-May-12
04-May-12
05-May-12
05-May-12
25-May-12
27-May-12
27-May-12
27-May-12
31-May-12
31-May-12
01-Jun-12
01-Jun-12
02-Jun-12
02-Jun-12
12-Jul-12
12-Jul-12
12-Jul-12 / 59
THAILAND / 29-Aug-11
21-Oct-11
24-Oct-11
24-Oct-11
24-Oct-11
24-Oct-11
24-Oct-11
12-Nov-11
12-Nov-11
23-Nov-11
23-Nov-11
14-Dec-11
18-Jan-12
18-Jan-12
26-Mar-12
26-Mar-12
26-Mar-12
30-Apr-12
02-May-12
04-May-12
08-May-12
18-May-12
18-Jan-12
30-Apr-12 / 24
TURKEY / 20-Oct-11
17-Nov-11
24-Nov-11
01-Dec-11
06-Dec-11
07-Dec-11
08-Dec-11
12-Dec-11
16-Dec-11
19-Dec-11
27-Dec-11
27-Dec-11
27-Dec-11
29-Dec-11
29-Dec-11
02-Jan-12
05-Jan-12
06-Jan-12
10-Jan-12
11-Jan-12 / 20
UNITED ARAB EMIRATES / 23-Dec-11 / 1
VIETNAM, SOCIALIST REPUBLIC OF / 04-Feb-08
18-Jul-08
22-Feb-11
10-Mar-11
21-Mar-11
28-Sep-11
28-Sep-11
01-Dec-11
01-Dec-11
05-Jan-12
16-Jan-12
03-Feb-12
04-Feb-12
06-Feb-12
22-Feb-12
14-Mar-12
14-Mar-12
14-Mar-12
14-Mar-12
14-Mar-12
24-Mar-12
24-Mar-12
16-Apr-12
16-Oct-12
16-Oct-12
16-Oct-12
16-Oct-12
16-Oct-12
16-Oct-12
05-Nov-12
05-Nov-12 / 31
ZAMBIA / 9

Additional Serotyping data for samples received to Pirbright Reference Laboratory for FMD 2012

Country / No. / Virus isolation in cell culture/ELISA / RT-PCR for FMD (or SVD)
of / FMD virus serotypes / SVD / NVD / NT / virus (where appropriate)
samples / O / A / C / SAT 1 / SAT 2 / SAT 3 / Asia 1 / virus / Positive / Negative / NT
AFGHANISTAN / 20 / 3 / 4 / - / - / - / - / 5 / - / 8 / - / 20 / - / -
BAHRAIN / 129 / 2 / - / - / - / 5 / - / - / - / 73 / 53 / 9 / 109 / 11
BHUTAN / 9 / - / - / - / - / - / - / - / - / 9 / - / - / 9 / -
BOTSWANA / 21 / - / - / - / - / 21 / - / - / - / - / - / 21 / - / -
EGYPT / 49 / 7 / 11 / - / - / 18 / - / - / - / 13 / - / 45 / 3 / 1
ERITREA / 18 / 15 / - / - / - / - / - / - / - / 3 / - / 16 / 2 / -
ETHIOPIA / 51 / 19 / - / - / - / - / - / - / - / 32 / - / 43 / 7 / 1
HONG KONG, SAR OF PRC / 3 / 1 / - / - / - / - / - / - / - / 2 / - / 3 / - / -
IRAN / 48 / 9 / 15 / - / - / - / - / 20 / - / 4 / - / 45 / 3 / -
ISRAEL / 2 / 2 / - / - / - / - / - / - / - / - / - / 2 / - / -
JAPAN / 1 / 1 / - / - / - / - / - / - / - / - / - / 1 / - / -
KENYA a / 23 / 9 / - / - / 1 / 10 / - / - / - / 6 / - / 18 / 5 / -
KUWAIT / 12 / 12 / - / - / - / - / - / - / - / - / - / 12 / - / -
LIBYA / 125 / 15 / - / - / - / 3 / - / - / - / 107 / - / 47 / 78 / -
MALAYSIA / 18 / 6 / 3 / - / - / - / - / - / - / 9 / - / 17 / 1 / -
PAKISTAN / 43 / - / 3 / - / - / - / - / 7 / - / - / - / 10 / - / -