Author Name, Date / Follow-up / Disease Activity Measure(s) Assessed / Statistical Analysis / Reported RBC MTXGlu Subtypes Correlated to Disease Activity Markers
RCT and Prospective Cohort Studies – RA Patients
Dervieuxet al 2006 / 6months / DAS28-CRP, physician assessment of disease activity / Generalised linear model / 3*
Stamp et al. 2011 / 24weeks / DAS28-ESR, physicians global score, CRP, pain, mHAQ / Spearmans rho / Concentration of 1, 2, 3, 4, 5, 1–5, 3-5
Hoblet al 2012 / 16weeks / DAS28-ESR1, duration of morning stiffness, mHAQ, pain and fatigue (10cm VAS) / Spearmans rho / Area under the concentration-time curve, maximal concentration, time to maximal concentration and half life of 1, 2 and 3
De Rotteet al 2013a (MTX-R) / 9months / DAS28, EULAR Response Criteria / Multivariate linear and logistic regression / Concentration of 1, 2, 3, 4 and 5 individually, and in quintiles and tertiles.
De Rotteet al 2013b (Rotterdam) / 9months / DAS28, EULAR Response Criteria / Multivariate linear and logistic regression / Concentration of 1, 2, 3, 4, 5 and 1–5.
Cross Sectional Studies – RA patients
Angelis-Stoforidiset al 1999 / unclear / Responder, partial responder or non-responder according to Physicians global clinical assessment (patient history, joint examination and comparative temporal assessment), In addition, ESR and CRP / t-test or Mann Whitney U Test / Total Concentration
Dervieuxet al 2004 / Single measure / 22 TJC and SJC, physicians global assessment, patient global assessment, mHAQ, physician assessment of response to MTX (10cm VAS) / Multivariate linear regression / 3
Dervieuxet al 2005 / Single measure / 22 TJC and SJC, physicians global assessment, mHAQ, physician assessment of response to MTX (10cm VAS) / Multivariate linear regression / 3
Stamp et al. 2010 / Single measure / DAS28-ESR, CRP, CDAI, SDAI, physician response to MTX, mHAQ, pain and fatigue (10cm VAS) / Spearmans rho and multivariate linear regression / Concentration of 1, 2, 3, 4, 5, 1–5, 3-5
Ando et al. 2013 / Single measure / DAS28-ESR and DAS28-CRP, patient assessment of pain (100-point VAS) / Linear regression / Concentration of 1, 2, 3, 4, 5 and presence of 4 and 5 according to DAS282.6 or DAS285.1
Prospective Cohort Studies – JIA Patients
BulatovicCalastanet al 2013 / 12months / Juvenile Arthritis Disease Activity Score (JADAS)-27, measured in 27 joints / Multivariate linear regression, ANCOVA and linear mixed-model analysis / 3, 4, 5, 1–5 and 3-5
Cross Sectional Studies – JIA patients
Dolezalovaet al 2005 / Single measure / number of active and limited joints, ESR, physicians global assessment, parents evaluation of general well-being / Not reported / Total Concentration
Becker et al. 2011 / Single visit with retrospective review at 6 and 12months / AJC, ESR and assessment of active arthritis / Multivariate logistic regression / Placed into 3 clusters according to pattern of polyglutamation.
Concentration of 1, 2, 3, 4, 5 and 3-5
NS – not stated, 1 – reported as primary outcome, Unclear=patients could have been assessed multiple times >1month apart
*presumably, based on previous studies by this group