These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.
Chemical Use in Animal Research ● Standard Operating Procedures LAB Name
Carcinogens: 7, 12-Dimethylbenz(A)anthracene (DMBA)
Date SOP Created: June 16, 2010
Instructions: Insert specific details pertaining to your research; contact EH&S at 642-3073 or OLAC at 642-9232 for assistance.
Human Health Hazard information / Provide known hazards to humans and physical description, color, odor characteristics, etc. Example: DMBA is highly carcinogenic in experimental animals. Large single and multiple doses produce tumors of the skin, breast, and stomach or leukemias regardless of route of administration. Skin of mice is particularly sensitive to low, topically applied doses.DMBA is a strong mutagen after metabolic activation.
DMBA is absorbed through the skin and respiratory and intestinal tracts; and by intravenous and
intraperitoneal injection, ingestion, and inhalation. It is carcinogenic and may irritate tissues and induce sensitivity. Avoid formation and breathing of dusts.
Laboratory operations should be conducted in a fume hood posted “Carcinogen in Use – restricted area”. Wash hands after handling material, treated animals, or soiled bedding and removing gloves.
Preparation / List procedures used to make the chemical solution. Be specific about the physical form (solid, liquid, etc.) and locations for work (bench top, fume hood, biosafety cabinet), and be very specific about personal protective equipment (PPE) to be worn when handling the material.
Include room number, use of fume hood, lab coat, nitrile gloves, wash hands after handling, etc, Solid DMBA and solutions are stored in dark-colored, tightly-closed containers.
Transportation /
Discuss the precautions that will be taken if the chemical is to be transported; if all work will be done within the same lab, this section is not applicable. Example: The solution is transported to Xin a primary container that is leakproof placed in suitable package for transport to Room X (fume hood required) along with a large clear carcinogen warning and contact information for the PI.
Use / List specifics regarding use of the chemical. Include information regarding PPE and location of work. Example: Treatment: To contain spills place a diaper on the fume hood work surface where topical application will be done for the animals. Animals are given a single injection of DMBA (consult with your protocol or vet for proper gauge needle). Animals that are receiving application of DMBA will be placed on the diaper in the fume hood to contain spills. Excess DMBA solution will be carried back to the lab in the same container and will be stored under the fume hood. The cages housing the animals must be clearly identified with the word Toxic, DMBA, date of treatment, and “Do not change cage”; and the OLAC staff must be aware of the presence of a toxic chemical in the cage, and that they must not change bedding for one week after the date of injection.Disposal
If unsure, contact EH&S at 642-3073 to determine if chemical disposal is necessary for bedding, unused drinking water, and/or carcasses following treatment. / Include language describing the proper method for disposing of unwanted waste. This waste may include chemically contaminated materials from the preparation of solutions; in some cases bedding and waste from the animal needs to be handled as hazardous waste. Example:
No waste streams containing DMBA shall be disposed of in sinks or general trash.
Chemical waste must be disposed by EH&S by completing an electronic Material Packing List (eMPL) form available at http://mpl.ehs.berkeley.edu/ and submitting it to EH&S. The unused portions of injectable solutions and of stock solutions will be brought back to Room X and disposed as liquid waste. Contaminated syringes and needles will be disposed immediately after use into appropriate sharps disposal containers. Double-bagged “Toxic Waste” container will be clearly marked and kept for daily generated dry waste (gloves, wipes, protective paper, soiled cage bedding etc.). When full it will be returned to Room X until pickup by EH&S.
The cage bedding must be changed by the researchers after treatment with DMBA for at least one week. Soiled bedding will be collected within a biosafety cabinet in red bag waste and deposited into the biohazardous waste red barrels in the cold room for weekly pick up by EH&S. Used cages will be wiped inside with a wetted paper towel (towels also should be discarded as biohazardous waste) two times, then delivered to OLAC for automatic cage washing.
Spill response and emergency procedures / Small amounts of dilute chemical should be readily absorbed by a spill pillow, paper towels or granular absorbent and immediately placed in a fume hood. Any waste generated should be disposed of through EH&S as chemical waste, see disposal section. A large spill of concentrated chemical can create a potentially dangerous level of airborne vapor. Absorbent material should be placed over the spill if possible and the lab should be evacuated of all personnel if necessary. For emergency clean up response or if there is an inhalation hazard, notify EH&S at 642-3073.
Hazard communication, signs, cage cards, etc. / All researchers handling this material should read and sign this document, be familiar with the lab’s Chemical Hygiene Plan and know how to access the MSDS. When animals have been treated with/administered this chemical, all cage cards should be labeled appropriately with the name of the carcinogen and the words “Carcinogen,” “Don not change cage” and this SOP should be posted in a plastic sleeve on the applicable cage rack or housing door to notify OLAC staff and other personnel. Also have the OLAC manager review and initial below prior to posting.
Unique instructions / Insert any specific precautions or procedures.
Additional information or references / Refer to the MSDS and TOMES chemical database available at http://www.ehs.berkeley.edu/healthsafety/msds.html, your lab’s chemical hygiene plan, or contact your supervisor or EH&S at 642-3073 for further guidance.
Additional guidance on handling and hazards of DMBA: (insert others if applicable)
http://www.hi.umn.edu/sop/RJM%20SOP%20for%20Hazardous%20Chemical%20DMBA.pdf
http://www.ehs.uci.edu/programs/sop_library/
http://ehs.georgetown.edu/chemsafe/forms/SOPTEMPLATES/DMBA.pdf
Training documentation (name and date):
Office of Environment, Health & Safety ● http://ehs.berkeley.edu/ ● 642-3073
OLAC Manager Initials/Date: ______Effective Dates: ______to ______
These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.
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Office of Environment, Health & Safety ● http://ehs.berkeley.edu/ ● 642-3073
OLAC Manager Initials/Date: ______Effective Dates: ______to ______