Basic Instrumental Analysis
COURSE OUTCOME
CHM 260
Basic Instrumental Analysis
Semester: July – Oct 2007
Text:Principles of Instrumental Analysis, 5th edition, Skoog, Holler & Nieman, Saunders, 1998
Goal:Enable students to understand and apply scientific principles and methods of chemical analysis.
Learning outcomes:
On completion of this course, students should be able to do the following:
1. Identify and explain the different types of spectroscopic methods of analysis.
2Identify and explain the different types of chromatographic methods of analysis.
3.Identify and explain the different types of electromagnetic radiation.
4.Explain the principles and the working mechanism of Ultraviolet visible(UV) Absorption Spectroscopy, Infrared Absorption (IR) Spectroscopy, Atomic Absorption (AA) Spectroscopy, Flame Emission (FE) Spectroscopy, Gas Chromatography (GC) and High Performance Liquid Chromatography (HPLC).
5.Draw the schematic diagram of the related instruments in separation and identification techniques and able to explain the function of each components of the instrument. Differentiate between different instruments in terms of parts and functions. (For example: radiation sources and sample cells, detectors)
6.Identify, distinguish and apply the appropriate chemical analysis methods in determining unknowns.
7.Be able to use different instrumental methods of analysis to solve for specific analytical problems. The student should be able to choose the appropriate instrumental method for a particular investigation.
8.Interpret and evaluate analytical information from spectral data and diagnose a physical problem and decide the most appropriate instrumental method for further investigation and quantify the known compound graphically and numerically.
9.Operate the UV, IR, AA, FE, GC, HPLC instruments with minimal supervision.
10.Acquire the science skills related to spectroscopic and chromatographic analysis.
LESSON PLAN
1 / 1
1
1 / Diagnostics and Learning Skills • Conceptual Survey in Chemical Concept Inventory• Learning Styles • Concept Mapping
Introduction to Spectroscopic Methods of Analysis
Fundamental principles
Properties of electromagnetic radiation
2 / 1
1 / Introduction to Spectroscopic Methods of Analysis
Molecular absorption spectroscopy.
Terms employed in absorption spectroscopy: Transmittance and Absorbance
Beers’ Law: Application of Beer’s Law to mixtures, limitations to the applicability of Beer’s law
3 / 1
1 / Ultraviolet/Visible Spectrometry and Photometry
Molecular species that absorb UV/Vis radiation: Organic and inorganic compounds
Instrumentation for UV spectroscopy
4 / 1
1 / Ultraviolet/Visible Spectrometry and Photometry
Application of absorption measurement to qualitative analysis
Quantitative analysis by absorption measurement
Test 1
5 / 1
1 / Infrared Absorption Spectroscopy
Molecular species that absorb IR radiation
Instrumentation and sample handling techniques
6 / 1
1 / Infrared Absorption Spectroscopy
Qualitative applications: Structural Analysis
Qualitative applications: Structural Analysis
7 / 1
1 / Atomic Spectroscopy based on Flame Atomization
Fundamental principle
Sample atomization, interferences
8 / 1
1 / Atomic Spectroscopy based on Flame Atomization
Instrumentation: Hollow cathode lamp
Quantitative analysis
9 / 1
1 / Flame Emission Spectroscopy
Fundamental principles
Comparison with atomic absorption
Test 2
10 / 1
1 / Introduction to Chromatographic Separations
Migration rates of solutes, column efficiency and resolution
Qualitative/quantitative analysis
11 / 1
1 / Gas Chromatography
Principles of GC
Instrumentation: Carrier gas, sample injection system, column temperature (isothermal & thermal programming), types of columns.
12 / 2 / Gas Chromatography
Detectors: principles and application for flame ionization detector, thermal conductivity detector, electron capture detector
13 / 1
1 / High Performance Liquid Chromatography
Principles of HPLC
Instrumentation: Mobile phase reservoirs and solvent system (isocratic and gradient elution), pumping systems, detectors
14 / 2 / High Performance Liquid Chromatography
Types of Liquid Chromatography (Adsorption, Partition – normal and reverse phase, ion exchange and size exclusion); Principles and applications
Test 3
15 / Revision
16 / FINAL EXAMINATION
Assessment:
Tests (3x):30%
Practical Reports:20%
Final Examinations:50%
References:
a)Spectrochemical Analysis, James D. Ingle, Jr. and Stanley R. Crouch
b)Introduction to Spectroscopy, 3rd edition by Pavia, Lampman, Kriz
c)Analytical Chemistry 6th edition , Gary D. Christian John Wiley &Sons, Inc.