Tables1phenotypic Characteristics of 12 Species of the Genus Aeromicrobium

TableS1Phenotypic characteristics of 12 species of the genus Aeromicrobium.

Taxa: 1, A. halocynthiae(Kim et al. 2010);2, A. ginsengisoli(Kimet al., 2008); 3, A. erythreum (Milleret al., 1991; Cuiet al., 2007); 4, A. ponti (Lee & Lee, 2008); 5, A. alkaliterrae (Yoonet al., 2005; Kim et al., 2008); 6, A. marinum (Brunset al., 2003; Yoon et al., 2005; Kim et al., 2008); 7, A. panaciterrae(Cuiet al., 2007); 8, A. tamlense (Lee & Kim, 2007); 9, A. fastidiosum(Collins & Stackebrandt, 1989; Tamura & Yokota, 1994; Yoonet al., 2005; Cui et al., 2007); 10, A. flavum(Tang et al., 2008). 11, A. massiliense (Ramasamy et al. 2012), 12, A. camelliae(Niu et al., 2015).

+, Positive reaction; w, weakly positive; -, negative reaction; ND, not determined.

* The results for taxa 12 were derived from our experiments.

† All data were from the above-mentioned literature for each type strain.

Table S2BiologGEN III microplate oxidation of carbon resource results of strain YIMY47T and the related referencestrain. Taxa: 1YIMY47T, 2. A. massilienseJC14T, + positive reaction, - negative reaction.

Table S3Biolog GEN III microplate chemical sensitivity assay results of strain YIMY47T and the related reference strain. Taxa: 1YIMY47T, 2. A. massilienseJC14T, + positive reaction, - negative reaction.

Table S4 Cellular fatty acid compositions (%) of strain YIMY47T and reference strain of the most closely related species of the genusAeromicrobium.Taxa: 1YIMY47T, 2. A. massilienseJC14T,ND: not detected. All data are from this study.

*Summed features are groups of two or three fatty acids that cannot be separated by GLC with the MIDI system. Summed feature 3 comprised C16:1ω7c and/or C16:1ω6c; Summed Feature 4comprised iso-I C17:1and/or anteiso-B C17:1; Summed Feature 6 comprised C19:1ω11c and/or C19:1ω9c; Summed Feature 7 comprised unknown 18.846 and/or C19:1ω6c; Summed Feature 8 comprised C18:1ω7c or C18:1ω6c; Summed Feature 9 comprised iso-C17:1ω9c or 10-methyl C16:0

Table S5API assay results of strain YIMY47Tand reference strain of the most closely related species of the genusAeromicrobium. All data are from this study.All of the strains,no acid is produced according to the results from the API 50CH tests.

Taxa: 1 YIMY47T, 2. A. massilienseJC14T, +, positive reaction; -, negative reaction;w,weakly positive.

Fig. S1Scanning electron micrograph of strain YIMY47Tafter growth on TSA medium at 30°C for 4 days, Bar, 5μm.

Fig. S2 Two-dimensional TLC analysis of the polar lipids withstrain YIMY47T and A.massilienseJC14T.

Abbreviations: DPGdiphosphatidylglycerol, PGphosphatidylglycerol,PIphosphatidylinositol,PL unknown phospholipid.

Fig. S3 Maximum-likelihood phylogenetic tree based on 16SrRNA gene sequences showing the relationships between strain YIMY47T and other members of the family Aeromicrobium. Bootstrap values (≥ 50 %) based on 1000 replications are shown at branch nodes. Bar, 0.01 substitutions per nucleotide position.

Fig. S4 Maximum-parsimony phylogenetic tree based on 16SrRNA gene sequences showing the relationships between strain YIMY47T and members of the family Aeromicrobium. Bootstrap values (≥ 50 %) based on 1000 replications are shown at branch nodes. Bar, 0.01 substitutions per nucleotide position.

YIMY47 (16SrRNA), GenBank Accession Number: KT023073

AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGTAAGGCCCCTTTCGGGGGGTACACGAGCGGCGAACGGGTGAGTAACACGTGAGCAACCTGCCCTTCTCTTTGGGATAACCAGTGGAAACGCTGGCTAATACCGAATATGACCGCCTTGGGCATCCGGGGTGGTGGAAAGTTCCGGCGGAGAAGGATGGGCTCGCGGCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCACCGACGAAGCGAAAGTGACGGTAGGTGCAGAAGAAGGACCGGCCAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTCTGTCGCGTCGGGAGTGAAAACTCGGGGCTTAACCCCGAGCGTGCTTCCGATACGGGCAGACTAGAGGGATGCAGGGGAGAATGGAATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGTTCTCTGGGCATTTCCTGACGCTGAGGAGCGAAAGCATGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCGCTAGGTGTGGGGACCTTCCACGGTTTCCGTGCCGCAGCTAACGCATTAAGCGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGCTGATTAATTCGATGCAACGCGAAGAACCTTACCTGGGTTTGACATATGCCGGAAAGCTGCAGAGATGTGGCCTCCCTTGTGGCCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTCCTATGTTGCCAGCACGTGATGGTGGGGACTCATAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGATGACGTCAAGTCTTCATGCCCCTTATGTCCAGGGCTTCAAGCATGCTACAATGGCCGGTACAATGGGCTGCGAAATCGTAAGGTGGAGCGAATCCCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCAACGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCATGAAAGTCGGCAACACCCGAAGCCGGTGGCCTAACCCTTGTGGGAGGAGCCGTCGAAGGTGGGGCTGGCGATTGGGACGAAGTCGTAACAAGGTAGCCGTAC