Table S1: Bacterial diversity from different hot springs studied previously

S. No. / Name of the hot spring / Sample type / Physiochemical conditions (temp., pH, altitude, trace elements) / Technique used / Dominant Phylum / Dominant genera / References
1 / Any Endre (Romania) / Microbial mat / 55°C, 8, Na+, K+, Ca+ / Unculturable / Euryarchaeota, Cyanobacteria, Chloroflexi / Methanomethylovorans
and Chloroflexus / [29]
2 / Rosiori (Romania) / Microbial mat / 45°C, 8, Na+, K+, Ca+ / Unculturable / Euryarchaeota, Cyanobacteria, Chloroflexi / Methanomassiliicoccus and Fischerella / [29]
3 / Siloam (South Africa) / Water with bulk of biofilm / 63°C, 9.5, 345 m HCO3-Na-Cl / Unculturable (454 sequencing) / Proteobacteria, Cyanobacteria, Bacteroidetes / Stenotrophomonas and Zavarzinella / [17]
4 / Mphephu / Water / 43°C, 8.19,850m, HCO3-Na-Cl / Unculturable (454 sequencing) / Proteobacteria, Cyanobacteria / Flectobacillus and Limnobacter / [30]
5 / Tshipise / Water / 58°C, 8.94, 188m, Cl-Na- HCO3 / Unculturable (454 sequencing) / Proteobacteria / Chloroplast GpIIb and Rheinhemera / [30]
6 / Sagole / Water / 45°C, 9.2, HCO3-Na-Cl / Unculturable (454 sequencing) / Proteobacteria / Rheinhemera and Vogesella / [30]
7 / Soutini / Water / 43.9°C, 7.20, Cl-Na-SO4 / Unculturable (454 sequencing) / Proteobacteria / Pseudomonas and Stenotrophomonas / [30]
8 / Eiland / Water / 43.9°C, 7.20, 420m, Cl-Na-SO4 / Unculturable (454 sequencing) / Proteobacteria / Stenotrophomonas and Hydrogenophaga / [30]
9 / Bakreshwar (India) / Water / 55°C, 9.2, 84m / Culturable / Firmicutes / Bacillus / [8]
10 / Balrampur (India) / Water / 90°C, 7.8, 623m / Culturable / Firmicutes / Bacillus / [8]
11 / Chumathang (India) / Water / 72°C, 8.4, 4023m / Culturable / Firmicutes, Proteobacteria and Actinobacteria / Bacillus / [8]
12 / Vashist (India) / Water / 47°C, 7.2, 1982m / Culturable / Firmicutes and Proteobacteria / Bacillus and Acinetobacter / [8]
13 / Tengchong / Microbial mat / 72°C, 6.5, 1520m / RFLP / Proteobacteria, Chlorobia, Firmicutes, Cyanobacteria / Leptolyngbya / [31]
14 / Langpu / Microbial mat / 65°C, 8.5, 1119m / RFLP / Aquificae and Proteobacteria / Deinococcus, Nitrospirae and Thermodesulfovibrio / [31]
15 / Borkhleung, Thailand / Microbial mat / 57°C, 6.8 / DGGE-PCR / Cyanobacteria and Bacteroidetes, / Thermosynechococcus and Roseiflexus related Chloroflexi / [32]

[29] Coman C, Drug B, Hegedus A, Sicora C, Drago N (2013) Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania. Extremophiles 17:523–534. doi:10.1007/s00792-013-0537-5

[30] Tekere M, Lotter A, Olivier J, Venter S (2015) Bacterial Diversity in Some South African Thermal Springs : A Metagenomic Analysis. Proceedings of the World Geothermal Congress, 2015:1–8.

[31] Pagaling E, Grant WD, Cowan DA, Jones BE, Ma Y, Ventosa A, Heaphy S (2012) Bacterial and archaeal diversity in two hot spring microbial mats from the geothermal region of Tengchong, China. Extremophiles, 16:607–618. doi:10.1007/s00792-012-0460-1

[32] Portillo MC, Sririn V, Kanoksilapatham W, Gonzalez JM (2009) Differential microbial communities in hot spring mats from Western Thailand. Extremophiles 13:321–331. doi:10.1007/s00792-008-0219-x

Fig. S1 Unrooted phylogenetic tree based on comparison of laccase conserved (142 bp) encoding gene fragment (a) and the corresponding amino acid sequences (b) from hot spring soil with their closest phylogenetic relatives. Phylogenetic tree was constructed based on aligned datasets using Neighbour joining (NJ) method and MEGA 5 software. Numbers on the tree indicate percentage of bootstrap sampling derived from 1000 random samples.