Supplementary table 1: Alcohol’s effects on µ-opioid receptor binding and coupling in wt and Arrb2 knockout mice .

[3H]-DAMGO binding / [35S]-GTPgS
baseline / [35S]-GTPgS
% stimulated
Mean / SEM / Mean / SEM / Mean / SEM
CgC / KO
WT / EtOH
Saline
EtOH
Saline / 167.55
208.43
176.04
171.77 / 6.47
12.25
6.55
15.95 / 239.58
255.64
232.89
215.64 / 9.83
13.32
25.24
25.86 / 21.99
23.91
21.98
22.39 / 0.21
0.51
2.23
0.51
AcbS / KO
WT / EtOH
Saline
EtOH
Saline / 238.69
328.70
302.07
289.93 / 17.71
52.48
21.20
27.28 / 842.62
712.34
475.43
743.35 / 76.25
29.47
21.08
97.34 / 69.23
66.30
38.66
63.84 / 4.15
3.25
2.68
6.52
AcbC / KO
WT / EtOH
Saline
EtOH
Saline / 261.25
307.91
274.76
279.07 / 9.26
73.99
3.38
17.16 / 821.78
803.03
566.28
731.47 / 91.14
39.43
68.72
43.62 / 73.81
75.36
51.11
62.93 / 7.29
4.77
5.11
0.46
CPu / KO
WT / EtOH
Saline
EtOH
Saline / 318.91
366.85
320.55
302.81 / 17.03
8.41
14.48
8.22 / 586.37
333.02
319.73
316.69 / 37.81
11.14
38.47
45.46 / 53.02
29.37
27.05
26.50 / 2.58
0.85
3.57
3.25
BNST / KO
WT / EtOH
Saline
EtOH
Saline / 77.06
31.64
46.67
37.95 / 14.20
10.50
5.14
4.31 / 1021.60.621.45
604.90
579.50 / 78.81
77.06
198.14
81.80 / 68.25
42.16
45.74
40.67 / 2.86
3.82
20.04
2.20
CeA / KO
WT / EtOH
Saline
EtOH
Saline / 90.15
63.90
66.69
65.78 / 3.06
1.13
4.18
2.63 / 1716.21
1476.07
1660.96
1501.58 / 180.83
86.10
139.46
62.44 / 54.09
42.97
36.43
42.90 / 1.28
3.65
2.77
3.03
MeA / KO
WT / EtOH
Saline
EtOH
Saline / 276.42
260.83
259.86
245.36 / 3.48
6.27
6.33
2.99 / 2082.61
2283.23
2010.27
1788.50 / 294.43
407.06
251.18
82.03 / 59.58
42.80
32.92
35.31 / 1.83
1.53
0.94
1.04
BLA / KO
WT / EtOH
Saline
EtOH
Saline / 180.25
183.87
171.74
168.55 / 2.56
4.55
4.66
2.52 / 1282.71
1144.68
1154.71
1164.53 / 147.28
79.40
73.79
112.95 / 43.68
41.03
35.35
30.29 / 2.43
3.56
1.61
2.16
BMA / KO
WT / EtOH
Saline
EtOH
Saline / 296.17
292.46
283.19
244.35 / 6.95
9.53
4.16
4.21 / 1633.96
1323.36
1535.12
1317.93 / 169.99
132.80
132.31
93.44 / 54.01
51.74
43.80
56.90 / 1.40
2.46
3.17
2.06
VTA / KO
WT / EtOH
Saline
EtOH
Saline / 139.46
133.27
121.04
130.91 / 13.57
18.15
4.67
3.01

KO - Arrb2 knockout mice WT-wildtype. Brain regions: mPFC – medial prefrontal cortex, AcbC –nucleus accumbens core regions, AcbS –nucleus accumbens nucleus, shell region, BNST – bed nucleus of the striaterminalis, CPu – caudate putamen, CeA, MeA, BLA, BMA – central, medial, basolateral and basomedial nucleus of the amygdala, respectively, VTA- ventral tegmental area.