Subject Experimental Research in Medical Chemistry
APSTIPRINĀTS
Rīgas Stradiņa universitātesCilvēka fizioloģijas un bioķīmijas katedras
Sanāksme 28.01.2016., protokola nr. 13.14.3/1
Subject“ experimental research in Medical chemistry ".
Time Lectures 12 hours;Practical work 20 hours.2 Credit points12-24 students in 1-2 groups.
Lecture Wensdayat 1615÷1745from 02.III to 14. VI or Wensday at 1600÷1730 from 02..III to 30. VI......
Prctical Class Wensday at 1615÷1830 from 02.III to 14.VIor Wensday at 1615÷1830 from 02.III to 9.VI....
Lecturesthen times inweeks 1.,2.,3.,4., 5.
Nr. / Week / Practical / Lecture / Nr. / Week / Practical1 / March18 / 2h / 8. / 16-20May / 2h
1. 2 / March25 / 2h / 2h / 9. / 23.-27. May / 2h
2. 3 / 01.-02.April / 2h / 2h / 10. / 01–04 June / 2h
3. 4 / 05.-09. April / 2h / 2h
4. 5 / 12.-16. April / 2h / 2h
5. 6. / 19.-30. April / 2h / 2h
6. / 02-06May / 2h / 2h
7. / 09-13May / 2h / 04. June 2016.Year / Wensday / Final class
Department Human Physiology and Biochemistry asist.professor Āris Kaksis: 08.jule 2015.year
1.-17.groupsSSNMF 10 students Gr.6, 9,14,
Experimental Research in Medical ChemistryLecture room Nr 406
Nr. / Week / Lectures Thursday 1615÷1745 24.IX÷22.X118. March Install applications on Your computer: Raswin2.6,ISIS Draw4,ChemScape, FireFo x3.5.5, Mage Windows in adress: avilable for MacOSX MacBook On Parallel Desktop: XP2 Windows Install applicationsRaswin2.6,ISISDraw4,ChemScape,FireFox3.5.5,Mage:
2. / 25.
March / Computer preparation for molecules experimental research. Instalalation.
Medical BioChemistry data base htdocs building and structure.
3. / 02.
April / Computer and FireFox3.5.5 configuration for molecular coordinates experimental research. Home page content in folder htdocs:... internet address:
4. / 09.
April / Molecules geometric research CO2,CO32,HCOOH,NH3,H2O, C2H4, C2H2, PCl5, [Al(OH)6]3- Task to molecules structure-geometry:
Molecular coordinates data research in angstroms for distance, bond angles and geometry
5. / 16.
April / 20 amino acid Fisher projections on pages to L-Amino acids
In Santa Barbara university given:
Isoelectric point pI calculation in myoglobine molecule pI=(pNKa+∑npKan+ pCKa)/(n+2)
6. / 16.
April / Guard Enzyme Glycoproteins immunoglobulin,immunological marker FUCose,
Nr. / Weak / Practical Lab. work 3 hours in week room Nr A406 Thursday 1615÷1830,
1. / 23.
oktober / Cell membrane Aquaporins 0,1 AQP0,AQP1Human HOMEOSTASIS physiology.
;
Aquaporins cell membranes crosing transport proteins of human body. Substrates waterH2O, oxygenO2, nitric oxideNO and enzyme inhibitor carbon mono oxide CO. All are channeling small neutral molecules:/Research/Aquaporine0.doc;/Research/Aquaporine1.doc
2. / 29-31.
oktober2 May / Carbonic Anhydrase CA: pH=7.36 determines ratio in expression of Henderson-Haselbalh7.36=pH=7.025+lg([HCO3]/[CO2]); CA ENZYME build by coordinative Zn2+ complex H2O+CO2=H++HCO3 Brensted acid H2O+CO2 base HCO3 alkaline reserve [HCO3]/[CO2]=2.036/1:
3. / 5.-9.
May / O2 Adsorbtion-Desorbtion Enzyme stored oxygen amount as blood reserve 459 times over and sensitive to blood plasma concentration [O2aqua]=6 10-5M what Myoglobin,Hemoglobin maintaines safe. Oxi-, deoxi-,carbomonoxi-CO & Fe+3metoxi-hemoglobin visible light specters. Chlorohemin crystalins. Triplet •:O≡:::≡O:• inactive safe oxygen stored and Singlet •::O-:-O::• activated oxygen: oxygen O2 and carbon dioxide CO2exchange ENZYMES Hemoglobin and CA:
4. / 12-16.
May / Heme coordinated iron(III) CATALASE HOMEOSTASIS activity in human organism
=Aexp(-Ea/RT) Activation energy Ea=29 J/mol, Bolcman factor exp(-Ea/RT) = 0,988
geometric factor A=0,13:
5. / 19-23.
May / /Research/NADalcoholDeHydr.doc:ENZYME alcohol dehydrogenase ADH. oxidise ethanol -CH2-OH to aldehyde -HC=O or ketone >C=O tuneling-transfer hydride ion Hwith proton H+ dissociation transfere to H2O:/ChemFiles/AlhoDeHydrogenase/NadDehydrogenase.htm
6. / 26-30.
May / /Research/PhosphLipidBilayerMembranB.doc Cell membrane structure of human physiology:
Cell membrane structure is water molecules and soluted compounds impermeable wall in organisms.
Normal ratio Cholesterol 0,9÷1/1 phospholipid in human erytrocites. Lipoproteins, Lipokalins:
1/3 cholesterols:1/3 phospholipids:1/3 proteins /Research/LipdBiLayerMemb.doc
7. / 03.-06. deceember / serum albumin HSA research HOMEOSTASIS physiology with Medical Chemistry. HSA lipoprotein: fatty acids, Hem, bilirubin and medicine like: aspirin, warfarin, ibuprofen, indometacin transporters throughblood plasma waterH2Omedium:
8. / 10.-11. deceember /
Nuclear receptor proteins: RORalpha HOMEOSTASISkey meaning in Cell regulation Cholesterol sulphate ester bound retinoic acid are orphan hormon to nuclear receptor:
androgen;
Mineral corticoid receptors complex Zn2+ finger motifs. Brane lysosome Cholesterol HOMEOSTASISmetabolism & transport class protein Lipocalin in water medium cytosol.
9. / 17.-18. deceember / Cyclo oxigenase human HOMEOSTASIS Physiology within Medical Chemistry. Enzyme protein research Task./Research/COX.doc: Triplet •:O≡:::≡O:• inactive safe and active
Singlet •::O-:-O::•oxygen Eicosatetraenoic acid sorce of:prostaglandinsPGs, prostacyclinsPGIs, thromboxanes TXs and leukotrienes LTs inhibitors: aspirin, warfarin, tylenol, paracetamol, ibuprofen:
10. / 07.-08. january / Genom HOMEOSTASIS instruments DNA methyl transferases:DNMT1HhaI;DNMT3GC ≡CG
Methylation Protein DNMT3experimental research task:/Research/DNAmethylTransferase.doc; Medical Chemistry Zn2+finger motifs: /hhaiDNAmethylCtransferKeny/methmast.htm
RSU dep. Human Physiology and Biochemistry responsible - assistent professor, Āris Kaksis.
Final classJanuary04.06.2016. on Wensday