RNA Gel Blots for Measuring the Levels of Endogenous Mir165/166 and STTM165/166-31In Arabidopsis

Supplementary Table 1 Primers used in this study

Supplementary Table 2 Predicted targets for miR858 in tomato by psRNATarget

Supplementary Figure Legend

Supplementary Figure S1. Multiple alignment and phylogenetic analysis of selected R2R3 MYB proteins. aAlignment of deduced amino acid sequences of SlMYB7-like with orthologs from Arabidopsis thaliana (AtMYB7) and Antirrhinum majus (AmMYB330). The conserved protein-protein interaction motif (([D/E]LX2[R/K]X3LX6L X3R) and the putative repressor motif (LNLE/DL)are indicated by a red and blue line, respectively. bThe phylogenetic tree of SlMYB7-like along with selected R2R3 MYBs was constructed using neighbor-joining method using MEGA5 software. The R2R3 MYB proteins, their respective plant species, andGenBank accession numbers are as follows: PhAn2, Petunia hybrida (AAF66727); NtAn2, Nicotiana tabacum (ACO52470);SlANT1, Solanum lycopersicum (AAQ55181); PAP1 (AAG42001), Arabidopsis thaliana; PAP2, A. thaliana (AAG42002); AmRosea, Antirrhinum majus (ABB83826); ZmC1, Zea mays (AAA33482); AtMYB123, A. thaliana (NP_179263); SlMYB7-like, S. lycopersicum (XP_004246088); AtMYB7, A. thaliana (NP_179263); AmMYB330, A. majus (MYB30_ANTMA).

Supplementary Figure S2.Map pOT2-STTM858 cloningvector and pC2300-pOT2-STTM858 binary vector over-expressing STTMmiR858.

Supplementary Figure S3.PCR analysis of STTM858 transgenic tomato lines. The numbers refer to the independent transgenic lines.

Supplementary Figure S4. Analysis of the expression levels of SlMYB12in STTM858 transgenic tomatoes.Relative expression of SlMYB12 in transgenic tomato lines was measured using qRT-PCR. SlCAC was used as an endogenous control for normalization. The expression level of each gene in the control is set to 1. Values represent mean ± SD of triplicate experiments from three independent biological samples.