Pgep-SD5 Restriction Map
pGep-SD5 (9571 bp)
- The pGep- retroviral cassette was from Phil Soriano (Chen, W.V. nature genetics 2004)
- The 3’LTR of pGep- do not include the gene supF. The viral promoter and enhancer are both deleted in the U3 region of the 3’ LTR to avoid potential interference with gene expression in the resulting provirus.
- The U3 region of 3’LTR is transfer to the 5’LTR upon provirus integration.
Plasmid Sequence:
gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcccaagaagacagtaagttccaaactctgggtgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagctctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgataccgtcgacTCGAGGCTAGAACTAGTGGATCCCCCGGGCTGCAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTCGCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGACCCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACGGTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAgaaggagtgagggctggataaagggaggatcgaggcggggtcgaacgaggaggttcaagggggagagacggggcggatggaggaagaggaggcggaggcttagggtgtacaaagggcttgacccagggaggggggtcaaaagccaaggcttcccaggtcacgatgtaggggacctggtctgggtgtccatgcgggccaggtgaaaagaccttgatcttaacctgggtgatgaggtctcggttaaaggtgccgtctcgcggccatccgacgttaaaggttggccattctgcagagcagaaggtaacccaacgtctcttcttgacatctaccgactggttgtgagcgatccgctcgacatctttccagtgacctaaggtcaaacttaagggagtggtaacagtctggcccatattctcagacaaatacagaaacacagtcagacagagacaacacagaacgatgctgcagcagacaagacgcgcggcgcggcttcggtcccaaaccgaaagcaaaaattcagacggaggcgggaactgttttaggttctcgtctcctaccagaaccacatatccctcctctaaggggggtgcaccaaagagtccaaaacgatcgggatttttggactcaggtcgggccacaaaaacggcccccgaagtccctgggacgtctcccagggttgcggccgggtgttccgaactcgtcagttccaccacgggtccgccagatacagagctagttagctaactagtaccgacgcaggcgcataaaatcagtcatagacactagacaatcggacagacacagataagttgctggccagcttacctcccggtggtgggtcggtggtccctgggcaggggtctcccgatcccggacgagcccccaaatgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagctcggggagcagaagcgcgcgaacagaagcgagaagcgaactgattggttagttcaaataaggcacagggtcatttcaggtccttggggcaccctggaaacatctgatggttctctagaaactgctgagggctggaccgcatctggggaccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattcagctgttccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattcagctgttccatctgttcctgaccttgatctgaacttctctattctcagttatgtatttttccatgccttgcaaaatggcgttacttaagctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaat4ggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga
Gep-SD5 (6368bp) Proviral Sequence:
tgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagcttaagctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgataccgtcgacTCGAGGCTAGAACTAGTGGATCCCCCGGGCTGCAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTCGCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGACCCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACGGTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAgaaggagtgagggctggataaagggaggatcgaggcggggtcgaacgaggaggttcaagggggagagacggggcggatggaggaagaggaggcggaggcttagggtgtacaaagggcttgacccagggaggggggtcaaaagccaaggcttcccaggtcacgatgtaggggacctggtctgggtgtccatgcgggccaggtgaaaagaccttgatcttaacctgggtgatgaggtctcggttaaaggtgccgtctcgcggccatccgacgttaaaggttggccattctgcagagcagaaggtaacccaacgtctcttcttgacatctaccgactggttgtgagcgatccgctcgacatctttccagtgacctaaggtcaaacttaagggagtggtaacagtctggcccatattctcagacaaatacagaaacacagtcagacagagacaacacagaacgatgctgcagcagacaagacgcgcggcgcggcttcggtcccaaaccgaaagcaaaaattcagacggaggcgggaactgttttaggttctcgtctcctaccagaaccacatatccctcctctaaggggggtgcaccaaagagtccaaaacgatcgggatttttggactcaggtcgggccacaaaaacggcccccgaagtccctgggacgtctcccagggttgcggccgggtgttccgaactcgtcagttccaccacgggtccgccagatacagagctagttagctaactagtaccgacgcaggcgcataaaatcagtcatagacactagacaatcggacagacacagataagttgctggccagcttacctcccggtggtgggtcggtggtccctgggcaggggtctcccgatcccggacgagcccccaaatgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagcttaagctagcttgccaaacctacaggtggggtctttca
Restriction Enzyme Map
Restriction Enzyme Map:
3’LTR
1 TGAAAGACCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAA 80
1 ACTTTCTGGGGGCGACTGCCCATCAGTTAGTGAGTCTCCTCTGGGAGGGTTCCTTGTCGCTCTGGTGTTCAGCCTACGTT 80
DdeI
81 CTGCAAGAGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCCCCGAGTGAGGGGTTGT 160
81 GACGTTCTCCCAAATAACCTATGTGCCCATGGGCCCGCTGAGTCAGTTAGCCTCCTGACCGCGGGGCTCACTCCCCAACA 160
RsaI HinfI NarI
KpnI DdeI
NciI
MspI
SmaI
NciI
161 GGGCTCTTTTATTGAGCTTAAGCTAGCTTGCCAAACCTACAGGTGGGGTCTTTCATTCCCCCCTTTTTCTGGAGACTAAA 240
161 CCCGAGAAAATAACTCGAATTCGATCGAACGGTTTGGATGTCCACCCCAGAAAGTAAGGGGGGAAAAAGACCTCTGATTT 240
AluI AluI
NheI
AluI
241 TAAAATCTTTTATTTTATCTATGGCTCGTACTCTATAGGCTTCAGCTGGTGATATTGTTGAGTCAAAACTAGAGCCTGGA 320
241 ATTTTAGAAAATAAAATAGATACCGAGCATGAGATATCCGAAGTCGACCACTATAACAACTCAGTTTTGATCTCGGACCT 320
RsaI PvuII HinfI EcoRII
AluI
321 CCACTGATATCCTGTCTTTAACAAATTGGACTAATCGATACCGTCGACTCGAGGCTAGAACTAGTGGATCCCCCGGGCTG 400
321 GGTGACTATAGGACAGAAATTGTTTAACCTGATTAGCTATGGCAGCTGAGCTCCGATCTTGATCACCTAGGGGGCCCGAC 400
EcoRV TaqI SalI TaqI SpeI BamHI NciI
TaqI Sau3AI SmaI
HincII DpnI NciI
HinfI MspI
XhoI
Human Adenovirus Tupe 3/5 SA splice site
401 CAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCG 480
401 GTCTAGACATCCCGCGTCATCAGGTCCCAAAGGAACTACTACAGTATGAATAGGACAGGGAAAAAAAAGGTGTCGAGCGC 480
PstI EcoRII AluI
BglII
Sau3AI
DpnI
-galactosidase
481 GTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATC 560
481 CAACTCCTGTTTGAGAAGCGCCAGAAAGGTCACCCCTAGCTGCCATAGCTATTCGAACTACTAGACACTGTACCGCCTAG 560
Sau3AI TaqI AluI Sau3AI BamHI
DpnI HindIII DpnI Sau3AI
TaqI DpnI
561 CCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCC 640
561 GGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGG 640
EcoRII
641 AGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGC 720
641 TCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGAAACG 720
PvuII Sau3AI BglI EcoRII
AluI DpnI
PvuI
721 CTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCT 800
721 GACCAAAGGCCGTGGTCTTCGCCACGGCCTTTCGACCGACCTCACGCTAGAAGGACTCCGGCTATGACAGCAGCAGGGGA 800
MspI MspI AluI Sau3AI DdeI
DpnI
801 CAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTT 880
801 GTTTGACCGTCTACGTGCCAATGCTACGCGGGTAGATGTGGTTGCACTGGATAGGGTAATGCCAGTTAGGCGGCAAACAA 880
881 CCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAAT 960
881 GGGTGCCTCTTAGGCTGCCCAACAATGAGCGAGTGTAAATTACAACTACTTTCGACCGATGTCCTTCCGGTCTGCGCTTA 960
HinfI AluI
961 TATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGC 1040
961 ATAAAAACTACCGCAATTGAGCCGCAAAGTAGACACCACGTTGCCCGCGACCCAGCCAATGCCGGTCCTGTCAGCAAACG 1040
HincII EcoRII
HpaI
1041 CGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGC 1120
1041 GCAGACTTAAACTGGACTCGCGTAAAAATGCGCGGCCTCTTTTGGCGGAGCGCCACTACCACGACGCGACCTCACTGCCG 1120
DdeI MspI
1121 AGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACA 1200
1121 TCAATAGACCTTCTAGTCCTATACACCGCCTACTCGCCGTAAAAGGCACTGCAGAGCAACGACGTATTTGGCTGATGTGT 1200
Sau3AI AatII
DpnI
1201 AATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCG 1280
1201 TTAGTCGCTAAAGGTACAACGGTGAGCGAAATTACTACTAAAGTCGGCGCGACATGACCTCCGACTTCAAGTCTACACGC 1280
RsaI
1281 GCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTC 1360
1281 CGCTCAACGCACTGATGGATGCCCATTGTCAAAGAAATACCGTCCCACTTTGCGTCCAGCGGTCGCCGTGGCGCGGAAAG 1360
1361 GGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTG 1440
1361 CCGCCACTTTAATAGCTACTCGCACCACCAATACGGCTAGCGCAGTGTGATGCAGACTTGCAGCTTTTGGGCTTTGACAC 1440
TaqI Sau3AI TaqI
DpnI
PvuI
1441 GAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCT 1520
1441 CTCGCGGCTTTAGGGCTTAGAGATAGCACGCCACCAACTTGACGTGTGGCGGCTGCCGTGCGACTAACTTCGTCTTCGGA 1520
HinfI
1521 GCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTT 1600
1521 CGCTACAGCCAAAGGCGCTCCACGCCTAACTTTTACCAGACGACGACGACTTGCCGTTCGGCAACGACTAAGCTCCGCAA 1600
HinfI
TaqI
1601 AACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCA 1680
1601 TTGGCAGTGCTCGTAGTAGGAGACGTACCAGTCCAGTACCTACTCGTCTGCTACCACGTCCTATAGGACGACTACTTCGT 1680
HincII EcoRV
HpaI
1681 GAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGT 1760
1681 CTTGTTGAAATTGCGGCACGCGACAAGCGTAATAGGCTTGGTAGGCGACACCATGTGCGACACGCTGGCGATGCCGGACA 1760
RsaI
1761 ATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCG 1840
1761 TACACCACCTACTTCGGTTATAACTTTGGGTGCCGTACCACGGTTACTTAGCAGACTGGCTACTAGGCGCGACCGATGGC 1840
SspI HinfI Sau3AI MspI
DpnI
1841 GCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGA 1920
1841 CGCTACTCGCTTGCGCATTGCGCTTACCACGTCGCGCTAGCATTAGTGGGCTCACACTAGTAGACCAGCGACCCCTTACT 1920
MluI Sau3AI BclI HinfI
DpnI Sau3AI
PvuI DpnI
1921 ATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAG 2000
1921 TAGTCCGGTGCCGCGATTAGTGCTGCGCGACATAGCGACCTAGTTTAGACAGCTAGGAAGGGCGGGCCACGTCATACTTC 2000
Sau3AI TaqI NciI
DpnI Sau3AI MspI
DpnI
2001 GCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCT 2080
2001 CGCCGCCTCGGCTGTGGTGCCGGTGGCTATAATAAACGGGCTACATGCGCGCGCACCTACTTCTGGTCGGGAAGGGCCGA 2080
RsaI NciI
BssHII MspI
2081 GTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGC 2160
2081 CACGGCTTTACCAGGTAGTTTTTTACCGAAAGCGATGGACCTCTCTGCGCGGGCGACTAGGAAACGCTTATGCGGGTGCG 2160
EcoRII Sau3AI
DpnI
2161 GATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCT 2240
2161 CTACCCATTGTCAGAACCGCCAAAGCGATTTATGACCGTCCGCAAAGCAGTCATAGGGGCAAATGTCCCGCCGAAGCAGA 2240
2241 GGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGAT 2320
2241 CCCTGACCCACCTAGTCAGCGACTAATTTATACTACTTTTGCCGTTGGGCACCAGCCGAATGCCGCCACTAAAACCGCTA 2320
Sau3AI
DpnI
2321 ACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAA 2400
2321 TGCGGCTTGCTAGCGGTCAAGACATACTTGCCAGACCAGAAACGGCTGGCGTGCGGCGTAGGTCGCGACTGCCTTCGTTT 2400
Sau3AI Eco47III
DpnI
PvuI
2401 ACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCG 2480
2401 TGTGGTCGTCGTCAAAAAGGTCAAGGCAAATAGGCCCGTTTGGTAGCTTCACTGGTCGCTTATGGACAAGGCAGTATCGC 2480
MspI TaqI
NciI
2481 ATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCA 2560
2481 TATTGCTCGAGGACGTGACCTACCACCGCGACCTACCATTCGGCGACCGTTCGCCACTTCACGGAGACCTACAGCGAGGT 2560
AluI
SacI
2561 CAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGT 2640
2561 GTTCCATTTGTCAACTAACTTGACGGACTTGATGGCGTCGGCCTCTCGCGGCCCGTTGAGACCGAGTGTCATGCGCATCA 2640
MspI MspI RsaI
NciI MluI
2641 GCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTG 2720
2641 CGTTGGCTTGCGCTGGCGTACCAGTCTTCGGCCCGTGTAGTCGCGGACCGTCGTCACCGCAGACCGCCTTTTGGAGTCAC 2720
MspI EcoRII DdeI
NciI
2721 TGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAG 2800
2721 ACTGCGAGGGGCGGCGCAGGGTGCGGTAGGGCGTAGACTGGTGGTCGCTTTACCTAAAAACGTAGCTCGACCCATTATTC 2800
TaqI
AluI
2801 CGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCG 2880
2801 GCAACCGTTAAATTGGCGGTCAGTCCGAAAGAAAGTGTCTACACCTAACCGCTATTTTTTGTTGACGACTGCGGCGACGC 2880
BglI
2881 CGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCG 2960
2881 GCTAGTCAAGTGGGCACGTGGCGACCTATTGCTGTAACCGCATTCACTTCGCTGGGCGTAACTGGGATTGCGGACCCAGC 2960
Sau3AI ApaLI EcoRII
DpnI TaqI
2961 AACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTG 3040
2961 TTGCGACCTTCCGCCGCCCGGTAATGGTCCGGCTTCGTCGCAACAACGTCACGTGCCGTCTATGTGAACGACTACGCCAC 3040
EcoRII ApaLI
3041 CTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAG 3120
3041 GACTAATGCTGGCGAGTGCGCACCGTCGTAGTCCCCTTTTGGAATAAATAGTCGGCCTTTTGGATGGCCTAACTACCATC 3120
MluI MspI MspI
3121 TGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGC 3200
3121 ACCAGTTTACCGCTAATGGCAACTACAACTTCACCGCTCGCTATGTGGCGTAGGCCGCGCCTAACCGGACTTGACGGTCG 3200
MspI PvuII
AluI
3201 TGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGT 3280
3201 ACCGCGTCCATCGTCTCGCCCATTTGACCGAGCCTAATCCCGGCGTTCTTTTGATAGGGCTGGCGGAATGACGGCGGACA 3280
3281 TTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGAC 3360
3281 AAACTGGCGACCCTAGACGGTAACAGTCTGTACATATGGGGCATGCAGAAGGGCTCGCTTTTGCCAGACGCGACGCCCTG 3360
Sau3AI RsaI
DpnI
3361 GCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGA 3440
3361 CGCGCTTAGCTTAATACCGGGTGTGGTCACCGCGCCGCTGAAGGTCAAGTTGTAGTCGGCGATGTCAGTTGTCGTTGACT 3440
HinfI HincII
TaqI
3441 TGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGT 3520
3441 ACCTTTGGTCGGTAGCGGTAGACGACGTGCGCCTTCTTCCGTGTACCGACTTATAGCTGCCAAAGGTATACCCCTAACCA 3520
TaqI NdeI
3521 GGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTG 3600
3521 CCGCTGCTGAGGACCTCGGGCAGTCATAGCCGCCTTAAGGTCGACTCGCGGCCAGCGATGGTAATGGTCAACCAGACCAC 3600
HinfI EcoRI PvuII MspI
EcoRII AluI
DdeI
PGK-1 promoter
3601 TCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGG 3680
3601 AGTTTTTATTATTATTGGCCCGTCCCCCCTAGGGGGTCTAGTAGCTTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTCC 3680
MspI BamHI Sau3AI EcoRI MspI
NciI Sau3AI DpnI NciI
DpnI TaqI
3681 CAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCAC 3760
3681 GTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGTGTGTAAGGTG 3760
SphI
3761 ATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGT 3840
3761 TAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAGGAGGGGATCAGTCCTTCA 3840
MspI NarI MspI
3841 TCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGAC 3920
3841 AGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTG 3920
AluI SpeI
3921 AGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCA 4000
3921 TCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCGAGT 4000
DdeI StuI BglI AluI DdeI
4001 GAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAG 4080
4001 CTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTC 4080
MspI DdeI DdeI NarI EcoO109I
NciI MspI
Neo*
4081 GCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGC 4160
4081 CGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCTGGACG 4160
NciI MspI TaqI
MspI NciI
4161 AGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCT 4240
4161 TCGGTTATACCCTAGCCGGTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGA 4240
PstI Sau3AI MspI
DpnI
4241 ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTT 4320
4241 TACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAA 4320
MspI NarI
NciI
MspI
4321 GTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGT 4400
4321 CAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCA 4400
MspI PstI
4401 TCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATC 4480
4401 AGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAG 4480
PvuII TaqI MspI Sau3AI
AluI NciI DpnI
4481 TCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCG 4560
4481 AGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGC 4560
Sau3AI
DpnI
MspI
4561 GCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCA 4640
4561 CGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGT 4640
TaqI TaqI RsaI MspI TaqI
Sau3AI
DpnI
4641 GGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG 4720
4641 CCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGC 4720
Sau3AI EcoRII BssHII SphI
DpnI
4721 ATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGAC 4800
4721 TACTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTG 4800
Sau3AI NcoI HinfI
DpnI TaqI
4801 TGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGA 4880
4801 ACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCT 4880
MspI AluI
4881 ATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG 4960
4881 TACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGC 4960
HinfI
Mouse Hprt SD splice site
4961 AGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTC 5040
4961 TCAAGAAGACTCCCCTAGTTAAGAGATCGCGCGGACTCATGAAGTCCCTAAACTTACATTCATTACGAAAAAAAAAAGAG 5040
DdeI Sau3AI BssHII ScaI
DpnI DdeI
RsaI
5041 GCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGAC 5120
5041 CGAGAGCAAAAAGTTTTTGGGTGTATTTTTAACTCCTTTCCCTTCTTAACAAAAGAGGAAGGTCGTGGAGCATTAAACTG 5120
5121 CCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACG 5200
5121 GGCTGACTACCAAGGGTAATCAGTGTATTTCGACATCAGTTCATGTCTGCAGGAATCTTGACCTTGAACCGGTCCGATGC 5200
AluI RsaI AatII EcoRII
DdeI
5201 GTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAGAAGGAGTGAGGGC 5280
5201 CACTGTGAAGAACGACCGACTTTATCAACTTGTCGAAATTATATCCGCGCGATCTCAGCTGGAGCTCTTCCTCACTCCCG 5280
AluI BssHII HinfI XhoI
SalI TaqI
TaqI
HincII
5281 TGGATAAAGGGAGGATCGAGGCGGGGTCGAACGAGGAGGTTCAAGGGGGAGAGACGGGGCGGATGGAGGAAGAGGAGGCG 5360
5281 ACCTATTTCCCTCCTAGCTCCGCCCCAGCTTGCTCCTCCAAGTTCCCCCTCTCTGCCCCGCCTACCTCCTTCTCCTCCGC 5360
Sau3AI TaqI
DpnI
TaqI
5361 GAGGCTTAGGGTGTACAAAGGGCTTGACCCAGGGAGGGGGGTCAAAAGCCAAGGCTTCCCAGGTCACGATGTAGGGGACC 5440
5361 CTCCGAATCCCACATGTTTCCCGAACTGGGTCCCTCCCCCCAGTTTTCGGTTCCGAAGGGTCCAGTGCTACATCCCCTGG 5440
DdeI RsaI EcoRII EcoRII EcoO109I
EcoRII
5441 TGGTCTGGGTGTCCATGCGGGCCAGGTGAAAAGACCTTGATCTTAACCTGGGTGATGAGGTCTCGGTTAAAGGTGCCGTC 5520
5441 ACCAGACCCACAGGTACGCCCGGTCCACTTTTCTGGAACTAGAATTGGACCCACTACTCCAGAGCCAATTTCCACGGCAG 5520
EcoRII Sau3AI EcoRII
DpnI
5521 TCGCGGCCATCCGACGTTAAAGGTTGGCCATTCTGCAGAGCAGAAGGTAACCCAACGTCTCTTCTTGACATCTACCGACT 5600
5521 AGCGCCGGTAGGCTGCAATTTCCAACCGGTAAGACGTCTCGTCTTCCATTGGGTTGCAGAGAAGAACTGTAGATGGCTGA 5600
PstI
5601 GGTTGTGAGCGATCCGCTCGACATCTTTCCAGTGACCTAAGGTCAAACTTAAGGGAGTGGTAACAGTCTGGCCCATATTC 5680
5601 CCAACACTCGCTAGGCGAGCTGTAGAAAGGTCACTGGATTCCAGTTTGAATTCCCTCACCATTGTCAGACCGGGTATAAG 5680
Sau3AI TaqI DdeI
DpnI
5681 TCAGACAAATACAGAAACACAGTCAGACAGAGACAACACAGAACGATGCTGCAGCAGACAAGACGCGCGGCGCGGCTTCG 5760
5681 AGTCTGTTTATGTCTTTGTGTCAGTCTGTCTCTGTTGTGTCTTGCTACGACGTCGTCTGTTCTGCGCGCCGCGCCGAAGC 5760
DdeI PstI
5761 GTCCCAAACCGAAAGCAAAAATTCAGACGGAGGCGGGAACTGTTTTAGGTTCTCGTCTCCTACCAGAACCACATATCCCT 5840
5761 CAGGGTTTGGCTTTCGTTTTTAAGTCTGCCTCCGCCCTTGACAAAATCCAAGAGCAGAGGATGGTCTTGGTGTATAGGGA 5840
5841 CCTCTAAGGGGGGTGCACCAAAGAGTCCAAAACGATCGGGATTTTTGGACTCAGGTCGGGCCACAAAAACGGCCCCCGAA 5920
5841 GGAGATTCCCCCCACGTGGTTTCTCAGGTTTTGCTAGCCCTAAAAACCTGAGTCCAGCCCGGTGTTTTTGCCGGGGGCTT 5920
DdeI ApaLI HinfI Sau3AI HinfI
DpnI DdeI
PvuI
5921 GTCCCTGGGACGTCTCCCAGGGTTGCGGCCGGGTGTTCCGAACTCGTCAGTTCCACCACGGGTCCGCCAGATACAGAGCT 6000
5921 CAGGGACCCTGCAGAGGGTCCCAACGCCGGCCCACAAGGCTTGAGCAGTCAAGGTGGTGCCCAGGCGGTCTATGTCTCGA 6000
EcoRII AatII MspI AluI
EcoRII NciI
6001 AGTTAGCTAACTAGTACCGACGCAGGCGCATAAAATCAGTCATAGACACTAGACAATCGGACAGACACAGATAAGTTGCT 6080
6001 TCAATCGATTGATCATGGCTGCGTCCGCGTATTTTAGTCAGTATCTGTGATCTGTTAGCCTGTCTGTGTCTATTCAACGA 6080
AluI RsaI
SpeI
5’LTR
6081 GGCCAGCTTACCTCCCGGTGGTGGGTCGGTGGTCCCTGGGCAGGGGTCTCCCGATCCCGGACGAGCCCCCAAATGAAAGA 6160
6081 CCGGTCGAATGGAGGGCCACCACCCAGCCACCAGGGACCCGTCCCCAGAGGGCTAGGGCCTGCTCGGGGGTTTACTTTCT 6160
AluI NciI EcoRII Sau3AI
MspI DpnI
NciI
MspI
6161 CCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAACTGCAAG 6240
6161 GGGGGCGACTGCCCATCAGTTAGTGAGTCTCCTCTGGGAGGGTTCCTTGTCGCTCTGGTGTTCAGCCTACGTTGACGTTC 6240
DdeI
6241 AGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCCCCGAGTGAGGGGTTGTGGGCTCT 6320
6241 TCCCAAATAACCTATGTGCCCATGGGCCCGCTGAGTCAGTTAGCCTCCTGACCGCGGGGCTCACTCCCCAACACCCGAGA 6320
RsaI HinfI NarI
KpnI DdeI
NciI
MspI
SmaI
NciI
6321 TTTATTGAGCTTAAGCTAGCTTGCCAAACCTACAGGTGGGGTCTTTCA 6368
6321 AAATAACTCGAATTCGATCGAACGGTTTGGATGTCCACCCCAGAAAGT 6368
AluI AluI
AluI
NheI
Enzymes that do not cut:
______
ApaI, DraI, MunI, NotI, NruI, NsiI, SacII, SnaBI, SrfI, VspI, XbaI, XmnI