MycorrhizaElectronic SupplementalMaterial

Article Title: Plant species differ in early seedling growth and tissue nutrient responses to arbuscular and ectomycorrhizal fungi

Authors: Ellen K. Holste1*, Richard K. Kobe1, and Catherine A. Gehring2

1 Department of Forestry, Michigan State University, Natural Resources Building,

480 Wilson Rd, Room 126, East Lansing, MI 48824, USA

2 Department of Biological Sciences and Merriam-Powell Center for Environmental Research, Northern Arizona University, 617 S. Beaver Street, Flagstaff, AZ 86011, USA

* Corresponding Author: ; Tel.: (517) 355-0090; Fax: NA

Online Resource 1. Percent fungal colonization (mean ± standard error) by tree species, fungal and nutrient treatments. Fungal colonizationvalues labeled with the same letter do not differ significantly (P<0.05) by ANOVA among treatments within each tree species.

Tree species / Fungal treatment / Nutrient
treatment / Fungal
colonization (%)
E. grandis / AMF / Low N and P / 10.10 ± 4.44 ab
AMF / High N and P / 14.83 ± 5.95 ac
AMF / Low N:P ratio (low N + high P) / 13.73 ± 4.83 ac
AMF / High N:P ratio (high N + low P) / 12.00 ± 6.17 ab
EMF / Low N and P / 4.42 ± 1.65 b
EMF / High N and P / 4.50 ± 1.34 b
EMF / Low N:P ratio (low N + high P) / 6.13 ± 1.47 b
EMF / High N:P ratio (high N + low P) / 7.78 ± 4.35 bc
Q. costaricensis / AMF / Low N and P / 8.91 ± 6.63 ab
AMF / High N and P / 14.11 ± 7.59 ab
AMF / Low N:P ratio (low N + high P) / 14.27 ± 6.26 a
AMF / High N:P ratio (high N + low P) / 6.88 ± 2.78 ab
EMF / Low N and P / 5.11 ± 2.74 b
EMF / High N and P / 9.29 ± 3.92 ab
EMF / Low N:P ratio (low N + high P) / 7.86 ± 2.09 ab
EMF / High N:P ratio (high N + low P) / 5.89 ± 2.31 ab

Online Resource 2. Shoot androot dry weight as functions of total plant dry weightby mycorrhizal fungal treatment (arbuscular (AMF) and ectomycorrhizal (EMF) fungi) and tree species. Fungal associated Eucalyptusgrandis (AMF and EMF) havesignificantly lower (a) shoot but greater (b) root dry weight than sterile-inoculatedplants (P<0.05), as indicated by different lettersnext to linear regression lines;conversely, allocation to (c)shoots and(d) roots in Quercuscostaricensisdo not significantly differ among fungal treatments (see Online Resource 3).

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Online Resource 3. Slope estimates, 95% confidence intervals, and coefficients of determination for plant components as functions of total plant dry weight based on linear regression models. Slope estimates labeled with the same letter do not differ significantly (i.e., 95% confidence intervals overlap by less than 29% indicating P < 0.05, in accordance with Austin and Hux 2002 and Kobe et al. 2010) among shoot or root dry weight allocation within tree species.

Tree species / Plant
component / Fungal treatment / Slope ± 95% CI / R2
E. grandis / Shoot / AMF / 0.52 ± 0.08 a / 0.81
Shoot / EMF / 0.54 ± 0.04 a / 0.94
Shoot / Sterile-inoculated / 0.66 ± 0.07 b / 0.88
Root / EMF / 0.46 ± 0.08 a / 0.93
Root / AMF / 0.48 ± 0.08 a / 0.79
Root / Sterile-inoculated / 0.34 ± 0.07 b / 0.66
Q. costaricensis / Shoot / AMF / 0.55 ± 0.06 a / 0.89
Shoot / EMF / 0.62 ± 0.07 a / 0.88
Shoot / Sterile-inoculated / 0.59 ± 0.06 a / 0.90
Root / AMF / 0.45 ± 0.06 a / 0.85
Root / EMF / 0.38 ± 0.07 a / 0.73
Root / Sterile-inoculated / 0.41 ± 0.06 a / 0.82

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Online Resource 4.Slope estimates, 95% confidence intervals, and associated statistics for tissue nutrient content (in grams of nutrient per foliar, stem or root tissue) as functions of plant dry weight(in grams of foliar, stem or root tissue) based on linear regression models. Slope estimates labeled with the same letter did not differ significantly (i.e., 95% confidence intervals overlap by less than 29% indicating P < 0.05, in accordance with Austin and Hux 2002 and Kobe et al. 2010) among foliar, stem, or root tissue nutrient contents within tree species.

Tree species / Tissue nutrient / Plant component / Fungal treatment / Slope ± 95% CI / R2
E. grandis / Nitrogen / Foliar / AMF / 1.79 ± 0.48a / 0.61
Nitrogen / Foliar / EMF / 0.71 ± 0.28b / 0.41
Nitrogen / Foliar / Sterile-inoculated / 1.57 ± 0.34 a / 0.68
Nitrogen / Stem / AMF / 1.00 ± 0.24a / 0.67
Nitrogen / Stem / EMF / 0.61 ± 0.20b / 0.55
Nitrogen / Stem / Sterile-inoculated / 0.85 ± 0.20ab / 0.70
Nitrogen / Root / AMF / 0.64 ± 0.09 a / 0.81
Nitrogen / Root / EMF / 0.52 ± 0.08 b / 0.81
Nitrogen / Root / Sterile-inoculated / 0.46 ± 0.12 c / 0.59
Phosphorus / Foliar / AMF / 0.15 ± 0.05a / 0.19
Phosphorus / Foliar / EMF / 0.07 ± 0.05b / 0.44
Phosphorus / Foliar / Sterile-inoculated / 0.11 ± 0.04 ab / 0.43
Phosphorus / Stem / AMF / 0.13 ± 0.04a / 0.57
Phosphorus / Stem / EMF / 0.14 ± 0.04a / 0.56
Phosphorus / Stem / Sterile-inoculated / 0.14 ± 0.02a / 0.78
Phosphorus / Root / AMF / 0.05 ± 0.02 a / 0.46
Phosphorus / Root / EMF / 0.05 ± 0.01 a / 0.67
Phosphorus / Root / Sterile-inoculated / 0.06 ± 0.02 a / 0.57
Sum of Base Cations / Foliar / AMF / 0.08 ± 0.01 a / 0.76
Sum of Base Cations / Foliar / EMF / 0.09 ± 0.01 a / 0.87
Sum of Base Cations / Foliar / Sterile-inoculated / 0.09 ± 0.02 a / 0.77
Sum of Base Cations / Stem / AMF / 0.04 ± 0.01 a / 0.78
Sum of Base Cations / Stem / EMF / 0.04 ± 0.01 a / 0.76
Sum of Base Cations / Stem / Sterile-inoculated / 0.04 ± 0.01a / 0.88
Sum of Base Cations / Root / AMF / 0.10 ± 0.01 a / 0.90
Sum of Base Cations / Root / EMF / 0.07 ± 0.01 b / 0.90
Sum of Base Cations / Root / Sterile-inoculated / 0.06 ± 0.01 b / 0.74

Online Resource 4 (cont).

Tree species / Tissue nutrient / Plant component / Fungal treatment / Slope ± 95% CI / R2
Q. costaricensis / Nitrogen / Foliar / AMF / 1.04 ± 0.11a / 0.92
Nitrogen / Foliar / EMF / 1.03 ± 0.15a / 0.86
Nitrogen / Foliar / Sterile-inoculated / 0.88 ± 0.13 a / 0.86
Nitrogen / Stem / AMF / 0.29 ± 0.07a / 0.70
Nitrogen / Stem / EMF / 0.31 ± 0.11a / 0.53
Nitrogen / Stem / Sterile-inoculated / 0.26 ± 0.08a / 0.56
Nitrogen / Root / AMF / 0.47 ± 0.09 a / 0.73
Nitrogen / Root / EMF / 0.39 ± 0.12 a / 0.52
Nitrogen / Root / Sterile-inoculated / 0.45 ± 0.10 a / 0.67
Phosphorus / Foliar / AMF / 0.07 ± 0.01a / 0.87
Phosphorus / Foliar / EMF / 0.08 ± 0.02a / 0.69
Phosphorus / Foliar / Sterile-inoculated / 0.08 ± 0.01a / 0.80
Phosphorus / Stem / AMF / 0.19 ± 0.03a / 0.86
Phosphorus / Stem / EMF / 0.20 ± 0.04a / 0.75
Phosphorus / Stem / Sterile-inoculated / 0.14 ± 0.05a / 0.57
Phosphorus / Root / AMF / 0.10 ± 0.03 a / 0.56
Phosphorus / Root / EMF / 0.11 ± 0.05 a / 0.35
Phosphorus / Root / Sterile-inoculated / 0.12 ± 0.04 a / 0.51
Sum of Base Cations / Foliar / AMF / 0.11 ± 0.01 a / 0.93
Sum of Base Cations / Foliar / EMF / 0.12 ± 0.02 a / 0.85
Sum of Base Cations / Foliar / Sterile-inoculated / 0.11 ± 0.02 a / 0.87
Sum of Base Cations / Stem / AMF / 0.04 ± 0.01 a / 0.79
Sum of Base Cations / Stem / EMF / 0.05 ± 0.01a / 0.72
Sum of Base Cations / Stem / Sterile-inoculated / 0.04 ± 0.01a / 0.69
Sum of Base Cations / Root / AMF / 0.05 ± 0.01 a / 0.92
Sum of Base Cations / Root / EMF / 0.04 ± 0.01 a / 0.75
Sum of Base Cations / Root / Sterile-inoculated / 0.05 ± 0.01 a / 0.84

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