Materials:5 Test Tubestest Tube Rackiodinebenedict S Solution Hot Plate Beaker Amylase Enzyme
Enzyme Lab
Background: Enzymes are proteins that speed up chemical reactions and are not consumed by the reaction. The most easily understood use of enzymes is in the breakdown or digestive process. Starch is a polysaccharide; composed of chains of simple sugars (monosaccharides). The enzyme Amylase, found in bean juice, breaks the chemical bonds in the starch chain releasing the simple sugars.
The presence of starch and simple sugar can be determined using an iodine test (starch) and a Benedict’s test (simple sugar).
Purpose: The purpose of this lab is to demonstrate enzyme activity in starch digestion, and to observe how changes in the temperature affect enzyme performance.
Hypothesis:
Materials:5 test tubestest tube rackiodineBenedict’s solution hot plate beaker Amylase enzyme
Procedure:(See Lab Handout)
1. Label test tubes 1 – 5.
2. To determine if simple sugar is present in starch solution place 3 drops of starch solution and 3 drops of Benedict’s in test tube 1. Heat in a water bath for 1 minute and record your result.
3. To determine the affect of Amylase enzyme on starch place 3 drops of starch solution and 3 drops of amylase into test tubes 2 and 3. Gently shake the tubes to mix. Wait 5 minutes.
4. After 5 minutes do a starch test (iodine) on tube 2 and a simple sugar test (Benedict’s) on test 3. Record your results.
5. Meanwhile to determine the affect of temperature increase on an enzyme place 3 drops of Amylase into test tube 4 and 5. Heat the tubes in a boiling water bath for 2 minutes then remove.
6. 3 drops of starch solution into test tubes 4 and 5, gently shake to mix and wait 5 minutes.
7. After 5 minutes do a starch test on tube 4 and a simple sugar test on test 5. Record your results.
Data:
Test Tube / Contents / Prediction (color) / Result (color) / Starch or Sugar Present? / Enzyme worked or didn’t?1 / STARCH and BENEDICTS
2 / STARCH and IODINE
3 / STARCH and AMYLASE, wait for five minutes, IODINE TEST
4 / STARCH and AMYLASE, wait five minutes, BENEDICT’S TEST
5 / AMYLASE, boil for two minutes, add STARCH, wait five minutes, BENEDICT’S TEST
6 / AMYLASE, boil for two minutes, add STARCH, wait five minutes, IODINE TEST
Analysis:
1. In complete sentences explain how you knew if starch contained sugar.
2. Starch is a complex carbohydrate consisting of a chain of glucose molecules. Sketch a starch molecule (at least 5 units long) and label the individual sugar molecules.
3. In complete sentences explain what the amylase was doing to the starch.
4. In complete sentences explain how heating the amylase affected its ability to react with starch.
Conclusion:
Follow your guidelines to write a complete “Conclusion.”