LOCUS Exported File 4930 Bp Ds-DNA Circular SYN 03-JAN-2013
LOCUS Exported File 4930 bp ds-DNA circular SYN 03-JAN-2013
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pTCP (empty vector)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4930)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Thursday, Jan 03, 2013 from SnapGene 1.4.0
FEATURES Location/Qualifiers
source 1..4930
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
/note="color: #ffffff; direction: RIGHT"
misc_feature 652..661
/note="Site of cDNA insert"
/note="color: #ff99cc"
rep_origin 815..1243
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 1257..1586
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 1598..2197
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/note="color: #ccffcc"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 2356..2477
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
primer_bind complement(2526..2542)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
rep_origin complement(2928..3513)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(3684..4544)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1:3684..4475/#ccffcc
2:4476..4544/#ccffcc/signalsequence
Cleavage site after base 4475"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4545..4649)
/gene="bla"
/note="AmpR promoter"
/note="color: #ffffff; direction: LEFT"
enhancer 4915..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
ORIGIN
1 tagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccg
61 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt
121 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca
181 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc
241 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
301 catgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattac
361 catggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggg
421 atttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacg
481 ggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgt
541 acggtgggaggtctatataagcagagctggtttagtgaaccgtggatcccgtcgcttacc
601 gattcagaatggttgatatccgccattctgaatcggtaagcgacgaagcttaataaagga
661 tcttttattttcattggatctgtgtgttggttttttgtgtgcggccgccctcgactgtgc
721 cttctagaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcg
781 gaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagc
841 gcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgccc
901 gctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagct
961 ctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaa
1021 aaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgc
1081 cctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaaca
1141 ctcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctat
1201 tggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgt
1261 gtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgc
1321 atctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagta
1381 tgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcc
1441 cgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttttta
1501 tttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggct
1561 tttttggaggcctaggcttttgcaaaaagctcccgggatgaccgagtacaagcccacggt
1621 gcgcctcgccacccgcgacgacgtcccgcgggccgtacgcaccctcgccgccgcgttcgc
1681 cgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccga
1741 gctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcgga
1801 cgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgtt
1861 cgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaaca
1921 gatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgt
1981 cggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagt
2041 ggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacct
2101 ccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggacc
2161 gcgcacctggtgcatgacccgcaagcccggtgcctgattcgaatgaccgaccaagcgacg
2221 cccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttc
2281 ggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggag
2341 ttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagc
2401 atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaa
2461 ctcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaa
2521 tcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacata
2581 cgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacatta
2641 attgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
2701 tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcg
2761 ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaag
2821 gcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaa
2881 ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctc
2941 cgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgaca
3001 ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg
3061 accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttct
3121 catagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt
3181 gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgag
3241 tccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagc
3301 agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctac
3361 actagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaaga
3421 gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtttttttgtttgcaag
3481 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg
3541 tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa
3601 aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata
3661 tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcg
3721 atctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgata
3781 cgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccg
3841 gctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct
3901 gcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt
3961 tcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc
4021 tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatga
4081 tcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagt
4141 aagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtc
4201 atgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaa
4261 tagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgcca
4321 catagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctca
4381 aggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatct
4441 tcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgcc
4501 gcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa
4561 tattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatt
4621 tagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtc
4681 gacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatg
4741 ccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcg
4801 cgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgc
4861 ttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacatt
4921 gattattgac
//