Legends for Supplementary Figures

Legends for Supplementary Figures

Supplementary Figure 1. Differential display profiles of RNA isolated from control and LT-treated leaves of Rheum. Arrows indicate the differentially expressed cDNA fragments that were used in the present work. C, control (25°C); LT, exposure of plants to 4°C for 4 h.

Supplementary Figure2. Nucleotide and deduced amino acid sequences of (A) RaMPK1 (GenBank accession number DQ078119) and (B) RaMPK2 (GenBank accession number DQ078123). The alternative polyadenylation signals are shown in bold underlined.

Supplementary Figure 3. Comparison of deduced amino acid sequences of (A)RaMPK1 and (B)RaMPK2 with MAPK homologs from other plant species using CLUSTAL W program. The sequences were aligned and gaps (dashes) introduced to maximize alignment. In the alignment asterisks (*) indicate perfectly conserved residues while dots (.) represent conserved amino acid substitutions. The 11 conserved sub-domains of protein kinases are indicated by Roman numerals above the sequences. The conserved TEYmotif is depicted by an arrow. The proline and glutamine rich region is shown by a line above the region. The GenBank accession numbers of MAPKs used for alignment are: GmMPK2 (AF329506), StMPK (AB062139), Ntf4-2 (DQ229078), LeMPK2 (AY261513), NtSIPK (U94192), PcMPK6 (AY173413), MsMMK1 (X66469), OsMPK6 (AB183398), AtMPK6 (AY120737), NbSIPK (AB098730), CsMPK (EF185418), NaWIPK (DQ991136), LeMPK3 (AY261514), NbWIPK (AB098729), CaMPK1 (AF247135), AtMPK3 (AF386961), BnMPK3 (AY642433), CbMPK3 (AY805424), PcMPK1 (Y12785), PsMPK3 (AF153061), PcMPK3b (AY173415), NtWIPK (D61377).

Supplementary Figure4. Predicted secondary structure of(A) RaMPK1; (B) RaMPK2, by SOPMA.Helices, sheets, turns, and coils are indicatedwith the longest, the second longest, the second shortest and the shortest vertical lines, respectively.

Supplementary Figure5. Hydropathy plots of (A) RaMPK2 and (B) RaMPK2

Supplementary Figure 3A.

Supplementary Figure 3B

Supplementary Figure 4A.

Supplementary Figure 4B.

Supplementary Figure 5A.

Supplementary Figure 5B.

Supplementary table 1. Primer sequences used in this study

Supplementary table 2: ESTs generated using DD