Fig. Icalibration Curves of Sixvalidation Standards Were Constructed at Six Concentration

SupplementalData

Fig. ICalibration curves of sixvalidation standards were constructed at six concentration levels (C1-C6: 0.01, 0.02, 0.1, 0.2, 1, 2 µg/ml) and the average peakarea oftriplicate measurements at eachconcentration level was calculated from the extracted ionchromatograms. A: PG(14:0/14:0); B: PE(14:0/14:0), C: PC(14:1/14:1); D: SPE(d17:0/12:0); E: LPG(17:1); F: LPE(17:1).

Table I721 lipid species, 415 identified by targeted MS/MS and 306 by highly accurate masses, were detected in rat peritoneal surface layers by our 2D-LC-MS method. Information on six exogenous lipids (validation standards, VS), was listed in this table as well.Lipids containing choline (PC, SM, and LPC) were best detected as [M+HCOO-]- and other lipids (PI, PG, PE, PS, FFA, LPI, LPG, LPE and LPS) were obtained with [M-H+]- as the molecular ions in negative mode.