Supplemental Table 1.Primers used in this study

Gene name
P. dolloi / Primer / Sequence (5'-3') / Application
3'Adaptor / CTCGAGATCGATGCGGCCGCTTTTTTTTTTTTTTTTTTTTTT / 3' RACE
3'Primer adaptor / CTCGAGATCGATGCGGCCGC / 3' RACE
TMPrimer / CAGGAAGAACARGRTRATRAA / qPCR
IgM1 / IgM1F0 / GCTTAGCAACAGACTACCTTCCAGG / qPCR
IgM1R0 / CCTGTGTACGTGCCTTTGTTAC / qPCR/ 5' RACE
IgM1F1 / CCACACTAATCAAATAAGGGAAG / 3' RACE
IgM1dolF2 / CGACCTCTGTCAATGTCACTC / 3' RACE
IgM1dolR2 / CTGTGAAAACAGCTGGGAATGC / 5' RACE
IgM2 / IgM2F0 / GCAAACCACTTGTTCCAGGGAG / qPCR
IgM2R0 / CATGTAAATTCTTCCTCAGAG / qPCR
IgM2F1 / GGTACATTCAGCATCATTAGC / 3' RACE
IgM2dolF2 / GGTCACTATCAAGAAGCACAATGG / 3' RACE
IgM2dolR1 / GGTTCAGAGCATGAAGCAACTGG / 5' RACE
IgM2dolR2 / GACTGCTGCCTATGTATGTTCCC / 5' RACE
IgW1s / IgW1sF0 / CCTGAAGTGTACAGCAATGGTCG / qPCR
IgW1sR0 / GTTGAAGTGCCTCTAAACCATGCC / qPCR
IgW1sdolFall / GACTTAACCTTACGTAATGAAATG / RT-PCR
IgW1sdolRall / GCTCAGATCAGTGGGACATTGG / RT-PCR
IgW1L / IgW1LF0 / CGACTGCCTACACCTGAAGAGG / qPCR
IgW1LR0 / GGTTGACCATGCAGGTATACG / qPCR / 5' RACE
IgW1LdolRall / GGCAATATGTACATTCAGTAAG / 5' RACE
IgW2L / IgW2LF0 / GCACAGAATGCCCAGAATACAC / qPCR / 3' RACE
IgW2LR0 / CAGTCCAGGTTATGTTAATATTGC / qPCR
IgW2LF1 / GACTGTGGACCAGAAACACCTAC / 3' RACE
IgW2LR1 / GCATTCTGGGCATTCTGTGC / 5' RACE
IgW2LR2 / GTAGCTTCATGCTTCACATCG / 5' RACE
IgN1 / IgN1F0 / CCACCATCTCTCCAAGTCCAAG / qPCR
IgN1R0 / CCTGTGTACGTGCCTTTGTTAC / qPCR/ 5' RACE
IgN1F1 / CCACCTTCCTTGTCCATCTTGG / 3' RACE
IgN1F2 / CCTTCATATCAAGAATTGGCCAG / 3' RACE
IgN1R2 / CCAAGATGGACAAGGAAGGTGG / 5' RACE
EF-1α / EF1aF / GGAAAGTCTCTCGAGGCAAGG / qPCR
EF1aR / CCATACCAGGTTTCAAGACACC / qPCR
Gene name
P. annectens / Primer / Sequence (5'-3') / Application
3'Adaptor / AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT / 3' RACE
3'Primer adaptor1 / AACTGGAAGAATTCGCGGCC / 3' RACE
3'Primer adaptor2 / AAGAATTCGCGGCCGCAGGAA / 3' RACE
IgM1 / 69944V-F1 / GTTCACCATCTCCCGTGACAAT / 3' RACE
LMGSP-F1 / TCGTTTGGTAAGAACTCAG / 5' RACE
LMGSP-F2 / AACACTGTTGGAGCTGCCTTCT / 5' RACE
M1S-F1 / AACCCAAGATTGTTCTTCTTCCAC / Southern blot
M1S-R1 / GATGCAGGTGAAGGAATGACCAT / Southern blot
IgM2 / 69974V-F1 / GCTCCTGGGAAAGGGCTTGAAT / 3' RACE
LM2GSP-F1 / GATCTCCTGGAAGGTAGTC / 5' RACE
LM2GSP-F2 / TGGAAGGTAGTCTGTTGCTAAG / 5' RACE
M2S-F1 / AACCCCAGATTTTCCTACTTCCA / Southern blot
M2S-R1 / TCTAAAGACTCATGACCGACAAT / Southern blot
IgM3 / 60639JF-1 / TGAATACTGGAGTCAAGGGAC / 3' RACE
LM3GSP-F1 / GTTACATAGCCTTCTGGGT / 5' RACE
LM3GSP-F2 / TAGCCTTCTGGGTCTATTTCA / 5' RACE
M3S-F1 / AGATTTTCCTTCTCCCACCTTCA / Southern blot
M3S-R1 / ACCGACAATGCAGGAGTAGGAAT / Southern blot
IgW1L / WL379-F1 / TTTGCGACTTGTCAGACTTTGC / 3' RACE
WL531-F2 / GATTGGAGTAATGGGATTGAGT / 3' RACE
LWGSP-F1 / AGTTACAAAGCAACCAATC / 5' RACE
LWGSP2-F2 / CAAAGCAACCAATCTTCAAAGG / 5' RACE
W1S-F4 / TTATATATGTCATCTTGTGACCCAG / Southern blot
W1S-R4 / TTTCAGAATGTGTAACTAGGCATTT / Southern blot
IgW2S / W-F1 / GCAACTCCTAGTGGACCTTTTGTGTAT / 3' RACE
LWGSP-F1 / AGTTACAAAGCAACCAATC / 5' RACE
LWGSP2-F2 / CAAAGCAACCAATCTTCAAAGG / 5' RACE
W1S-F4 / TTATATATGTCATCTTGTGACCCAG / Southern blot
W1S-R4 / TTTCAGAATGTGTAACTAGGCATTT / Southern blot
IgN1 / N1-F1 / AACCTGGATGAAAGATGGCAAG / 3' RACE
N1-F2 / AGCAGACAAAGAGGATGAGCCA / 3' RACE
GSPN1-1 / ATTCTGGTACAGTGATAAC / 5' RACE
GSPN1-2-1 / TTAGAATGATTGCCCACTTTACA / 5' RACE
GSPN1-2-2 / GTCCAGGTCACAGGCAGGTACTT / 5' RACE
GSPN1-2-3 / TACCCCAGTTGTAACTGGACCAG / 5' RACE
1281J-F1 / GGAAAGGGACCTTTGTCACC / RT-PCR
GSPN1-2(146) / TCTCTTTTAAGGTCCCATCCAA / RT-PCR
N1S-F1 / ATAGGATATATTGGCTGCCTGGT / Southern blot
N1S-R1 / ACAACTTTAGAATGACTGCCCAC / Southern blot
IgN2 / N2-F1 / AACCTGGATGAAAGATGGCAAG / 3' RACE
N2-F2 / AGCAGACAAAGAGGATGAGCCA / 3' RACE
GSPN2-1 / CAACTTTAGAATGACTGCC / 5' RACE
GSPN2-2-1 / GGTCACAGACAGGCACTTTCAAT / 5' RACE
GSPN2-2-2 / AGACAGGCACTTTCAATGAAGAA / 5' RACE
GSPN2-2-3 / CTGTAATTGGACCAGAAGATGCTT / 5' RACE
12714J-F1 / GATTACTGGGGACAAGGAACC / RT-PCR
GSPN2-2(146) / TCTCTTTTAAGGTCCCATCCAA / RT-PCR
N2S-F1 / ATAGGATATATTGGCTGCCTGGT / Southern blot
N2S-R1 / ACAACTTTAGAATGACTGCCCAC / Southern blot
IgN3 / N3-F1 / AACCTGGATGAAAGATGGCAAG / 3' RACE
N3-F2 / AGCAGACAAAGAGGATGAGCCA / 3' RACE
GSPN3-1 / ATTTACACTTGTAGCTCTT / 5' RACE
GSPN3-2-1 / GAGTAACTGCCACCGACATAAAC / 5' RACE
GSPN3-2 / AAGATGATTCCCATGATGCGTAA / 5' RACE
GSPN3-2-2 / TTTGGATCTGAGAGACCTGTGAC / 5' RACE
18793J-F1 / GGATTACTGGGGACAAGGAACC / RT-PCR
GSPN3-2(146) / TCTCTTTTAAGGTCCCATCCAA / RT-PCR
N3S-F1 / GATACATGCCCCTCTGTTTTTCC / Southern blot
N3S-R1 / CTTCTGACACACTGGAACAGCTT / Southern blot
IgQ / Q-F1 / AGTTGACAGTGCTGGGAAATAC / 3' RACE
Q-F2 / CATCTGACTGGAGCAAAGGAAG / 3' RACE
GSPQ-1 / GGACCAAAGTTTGTAATA / 5' RACE
GSPQ-2-1 / TGAAATAAGAATCCCATCAATGT / 5' RACE
GSPQ-2-2 / TAAAAGGCTGGGATAAAATTCAG / 5' RACE
GSPQ-2-3 / TTAGGCAATTGATGATGTCTGGT / 5' RACE
1281J-F1 / GGAAAGGGACCTTTGTCACC / RT-PCR
GSPQ-137 / TGCTCCAGTCAGATGGCAGAA / RT-PCR
VH2 family / V2S-F2 / GGGTATTTGGAAGCCTGGAGAT / Southern blot
V2S-R2 / AGTAATACAGGGCGCTGTCTTC / Southern blot
VH family specific sense primer / VH1-54-F1 / CAGACTGACCTGTAATGCCTCC / 1r primer
VH1-145-F2 / TGGAAAGGGACTCGAATGGG / 2r primer
VH2-27-F1 / GGGTATTTGGAAGCCTGGAGAT / 1r primer
VH2-54-F1 / CACCCTGACTTGTCGCTGTT / 2r primer
VH3-3-F1 / CATCGTGTTGACCCAGTCCG / 1r primer
VH3-100-F2 / ATGGAATGGGTGAGGCAGTT / 2r primer
VH4-36-F1 / ATCACCAGGCAGTACCATTG / 1r primer
VH4-100-F2 / GTAAGCTGGGTGCGACAAAC / 2r primer
VH5-137-F1 / GACTGGATTGGGTCGCCTAT / 1r primer
VH5-237-F2 / TCCCCAAAGGCTCCTCTAC / 2r primer
VH6-44-F1 / GAGACACGACCCGTCTGACCT / 1r primer
VH6-208-F2 / TCACGAGATGTCCCCAAAG / 2r primer
VH7-14-F1 / CCCAGTCTGAGGCCGAAGT / 1r primer
VH7-199-F2 / TTTTCGGCATCCAAGGACAGT / 2r primer
VH8-110-F1 / TCCGACAGCATCAAGGGAAAG / 1r primer
VH8-124-F2 / GGGAAAGGGCTCGAATTGTTA / 2r primer
VH9-21-F1 / CGGTGGAGACGTTAAAAGACC / 1r primer
VH9-52-F2 / CTCAGACTGACCTGCACAACC / 2r primer
VH10-36-F1 / GAAGCCTGGTGACACTTTGCG / 1r primer
VH10-123-F2 / AGGGAAGGGGTTGGTTTGG / 2r primer
VH11-13-F1 / AATCAGCCTGGTCCTGAAATT / 1r primer
VH11-52-F2 / GTCAGGCTGTCCTGCAAAGTG / 2r primer
VH12-6-F1 / AACGCTGAAGGAGACTGGAGG / 1r primer
VH12-207-F1 / AACCAAGGATAACTCCAAAGGG / 2r primer
VH13-44-F1 / GGGACAACATCATGCTGACCTG / 1r primer
VH13-197-F2 / TCAAAGTTCGGGTCACCATC / 2r primer
VH14-32-F1 / CGGGTCAGTCCCTGAAGCTA / 1r primer
VH14-111-F1 / TCCAGGGAAGGGGCTTGAGT / 2r primer
VH15-34-F1 / GGAGCCTCTGTGACCCTACA / 1r primer
VH15-114-F2 / AGGAACGGCACAGATGGAAT / 2r primer
VH16-7-F1 / GAGTCTGGAGGAGATGTGGTGA / 1r primer
VH16-111-F2 / TGCTGGTAAAGGGTTAGACTGG / 2r primer
V17-54-F1 / CCGGTTGAACTGCAAGGTGT / 1r primer
V17-115-F2 / CAGCCACCGGGTCAGACATT / 2r primer
Ig CH specific antisense primer / M1C-606-R1 / GTATGTTCCCTTGGCGCTTAT / 1r primer
M1C-572-R2 / GTCTTCGGCAGAAAGAGTGAG / 2r primer
M2C-1222-R1 / CGGTAGGGCTGTCAAGCTCATAT / 1r primer
M2C-784-R2 / ACTGCCCACAGCCAGAAGTAAAT / 2r primer
M3C-1246-R1 / CGACAGCAGCCTCCGTTTCATA / 1r primer
M3C-779-R2 / GCCACTGGAGGAAGAATGAACAC / 2r primer
W1l-964-R1 / TTCTCCAGTCCGCTATTGATAT / 1r primer
W1l-664-R2 / CAATCCCATTACTCCAATCCTCT / 2r primer
W2s-551-R1b / TACTCCGCTCCCTGATCCCAC / 1r primer
W2s-307-R2b / TGGTTGGACACCCACAATCTC / 2r primer
N1-810-R1 / CCAGATAATTTCCACTCCTCGT / 1r primer
N1-661-R2 / TTATCTTCCCAACTGTTGCTTT / 2r primer
N2-1482-R1 / ATTTATCTTTGCGCTGGTGGC / 1r primer
N2-616-R1 / AACCTGAAATTGTTTTGGAAGTCTT / 2r primer
N3-784-R1 / TATCATGCCCAGCAAACCACTT / 1r primer
N3-455-R2 / GAGTAACTGCCACCGACATAAA / 2r primer
Q-758-R1 / TAATAGTCAGGACGAGGACACC / 1r primer
Q-738-R2 / CCTCCTAAGATACCAGAAACCC / 2r primer