Appendix Results of Anovas for the Quantities of the 20 Most Abundant Epicuticular Hydrocarbon

Appendix Results of ANOVAs for the quantities of the 20 most abundant epicuticular hydrocarbon components in this study. Results are based on flies reared on defatted culture medium plus 1, 3, or 9% concentrations of triolein, tristearin, or tripalmitolein alone or combined. Controls (standard and defatted media) were not included in these analyses. Chemical names and equivalent chain lengths (in parentheses) are from Etges and Jackson (2001).

A. n-Octacosane (C28)B. 2-methyloctacosane (C28.65)

SourcedfType III SS FPr > FType III SS FPr > F

Population1192.2557.380.010 9908.25520.36< 0.001

Triacyglycerol (TAG)3 58.9480.750.52712692.473 8.69 <0.001

Concentration2133.2312.560.092 4640.485 4.77 0.015

Sex1 0.0380.000.970 193.026 0.40 0.533

Population X TAG3177.9082.280.097 514.417 0.35 0.788

Population X Sex1 12.3850.480.495 3009.742 6.18 0.018

TAG X Sex3164.3052.100.118 1360.006 0.93 0.436

TAG X Concentration5164.2301.260.302 4768.363 1.96 0.109

Pop X TAG X Sex3211.8882.710.060 794.954 0.54 0.655

Error35911.412 17035.791

C. 2-Methyltricontane (C30.65)D. 7- and 9-Hentricontene (C30.78)

SourcedfType III SS FPr > FType III SS FPr > F

Population1 791.8430.490.489 60.6222.990.093

Triacyglycerol (TAG)319274.0603.960.016353.7055.820.003

Concentration2 3592.3871.110.342 6.9670.170.843

Sex1 7.1090.000.948 40.1001.980.168

Population X TAG3 464.0000.100.962220.0483.620.022

Population X Sex1 6861.3204.230.047101.7985.020.031

TAG X Sex3 3995.3260.820.491 74.8021.230.313

TAG X Concentration510501.6921.290.289 95.3240.940.467

Pop X TAG X Sex3 3870.3410.800.505 19.7970.330.807

Error3556790.201709.131

E. Unknown (C33br1)F. 11- and13-Methyldotrocontane (C33br2)

SourcedfType III SS FPr > FType III SS FPr > F

Population1413.72828.49< 0.001626.35828.15 < 0.001

Triacyglycerol (TAG)3140.3373.220.034133.5352.000.132

Concentration2 45.2751.560.225 52.6771.180.318

Sex1 0.1460.010.921 9.2920.420.522

Population X TAG3 70.2271.610.204 43.8830.660.584

Population X Sex1 2.8140.190.663 1.8460.080.775

TAG X Sex3 32.9780.760.526159.7882.390.085

TAG X Concentration5 90.6481.250.308129.0021.160.348

Pop X TAG X Sex3 7.5510.170.914 68.5571.030.393

Error35508.304778.788

G. 31-Methyldotricont-8-ene (C32.47)H. 8, 24-Tricontadiene (C32.63)

SourcedfType III SS FPr > FType III SS FPr > F

Population11052.1231.730.197 6489.627105.34 < 0.001

Triacyglycerol (TAG)33359.3531.840.158 1792.666 9.70 < 0.001

Concentration2 540.7510.440.645 33.344 0.270.765

Sex1 101.2550.170.686 81.322 1.320.258

Population X TAG3 617.1170.340.798 1277.363 6.910.001

Population X Sex1 709.5241.170.288 202.971 3.290.078

TAG X Sex31849.8661.010.399 40.039 0.220.884

TAG X Concentration52305.4240.760.587 343.740 1.120.370

Pop X TAG X Sex3 805.1810.440.725 61.085 0.330.803

Error3521312.3962156.28599

I. 7, 25-Tricontadiene (C32.70)J. 10-, 12-, and 14-Tritricontene (C32.79)

SourcedfType III SS FPr > FType III SS FPr > F

Population12911.9669.390.0041922.79528.07 < 0.001

Triacyglycerol (TAG)33963.6604.260.0121265.3236.160.002

Concentration2 361.4950.580.564 186.1221.360.270

Sex1 30.1950.100.757 95.3911.390.246

Population X TAG32137.6372.300.095 676.0403.290.032

Population X Sex11282.0074.140.050 133.8801.950.171

TAG X Sex31362.0911.460.241 569.4192.770.056

TAG X Concentration5 579.1230.370.863 276.7991.350.275

Pop X TAG X Sex3 657.7070.710.554 357.3721.040.408

Error3510851.2702397.272

K. 8,26-Tetracontadiene (C34diene1) L. 6,26 and 6,24-Tetracontadiene (C34diene2)

SourcedfType III SS FPr > FType III SS FPr > F

Population1 58.19016.64<0.001439.3656.590.015

Triacyglycerol (TAG)3 56.3585.37 0.004628.4413.140.039

Concentration2 15.4562.21 0.126173.1471.300.287

Sex1 23.9676.85 0.013639.1919.590.004

Population X TAG3 11.9193.41 0.029519.8782.600.069

Population X Sex1 6.7701.94 0.174 90.4931.360.253

TAG X Sex3 25.8232.46 0.081210.4481.050.383

TAG X Concentration4 55.2173.95 0.010197.2790.740.572

Pop X TAG X Sex3 21.5482.05 0.126245.5321.230.316

Error32111.9112133.666

M. 10-, 12-, and 14 Tetratricontene (C34ene) N. 33-Methyltetratricont-10-ene (C35ene1)

SourcedfType III SS FPr > FType III SS FPr > F

Population1320.4397.410.010 83.9244.900.034

Triacyglycerol (TAG)3260.3252.010.133245.2244.770.007

Concentration2 25.8820.300.743 59.8941.750.190

Sex1 65.1221.510.229 3.2830.190.665

Population X TAG3141.8951.090.366 23.5240.460.714

Population X Sex1 24.5300.570.457 1.0990.060.802

TAG X Sex3108.6550.840.483126.3592.460.081

TAG X Concentration4 17.5230.100.981334.3224.880.004

Pop X TAG X Sex3219.1761.690.189 5.1370.100.960

Error321382.898548.361

O. 33-Methyltetratricont-8-ene (C35ene2)P. 9,25-Pentatricontadiene (C34.59)

SourcedfType III SS FPr > FdfType III SSFPr > F

Population1 362.6098.770.0061138957.04862.77< 0.001

Triacyglycerol (TAG)3 646.7915.220.005335532.318 5.35 0.004

Concentration2 161.0371.950.159260675.59913.70< 0.001

Sex1 63.0561.530.2261 3773.200 1.70 0.200

Population X TAG3 23.0960.190.905322247.384 3.35 0.030

Population X Sex1 0.0030.000.9931 5663.540 2.56 0.119

TAG X Sex3 239.2151.930.1453 1792.964 0.27 0.847

TAG X Concentration4 616.5293.730.013555427.740 5.01 0.002

Pop X TAG X Sex3 48.6790.390.7593 5206.237 0.78 0.511

Error321322.8623577481.117

Q. 8,26 and 7,27-Pentatricontadiene (C34.66)R. 9,27-Heptatricontadiene (C36.5)

SourcedfType III SS FPr > FType III SSFPr > F

Population1 54219.9322.060.1601693.79627.19< 0.001

Triacyglycerol (TAG)3210442.0802.670.063 957.8265.120.005

Concentration2100690.6261.920.162 385.2473.090.058

Sex1 3323.3740.130.724 22.9840.370.548

Population X TAG3111752.6111.420.254 189.0461.010.399

Population X Sex1 34822.2441.330.257 0.0030.000.995

TAG X Sex3 44699.1220.570.640 218.0591.170.336

TAG X Concentration5 51126.4070.390.853 346.6811.110.371

Pop X TAG X Sex3 83506.4611.060.379 57.8100.310.819

Error35919009.5212180.654

S. 8,28-Heptatricontadiene (C36.6)T. 14-, 16-, and 12-Hexatricontene (C36.7)

SourcedfType III SS FPr > FType III SSFPr > F

Population1 41.9820.170.685 1753.4295.060.031

Triacyglycerol (TAG)36209.8118.27<0.001 2757.7872.650.064

Concentration24803.5929.59<0.001 818.2141.180.319

Sex1 471.0601.880.179 1066.7213.080.088

Population X TAG31640.8642.180.107 1835.8311.760.172

Population X Sex1 33.0770.130.718 954.5862.750.106

TAG X Sex3 712.8910.950.428 1416.0121.360.271

TAG X Concentration54618.9143.690.009 971.3060.930.435

Pop X TAG X Sex3 530.4940.710.555 859.7150.500.777

Error358762.62212140.319