SupplementaryTable 1The markers linked to theBror gene

Primer / Forward primer (5′→3′) / Reverse primer (5′→3′)
BrID10733 / TTCTCTGTTCTCATGCTGTG / CGATTTGAAAAGTTTCGAGC
BrID120681 / CAGATGGATCTGCATGAAA / GAAAACCTGTCTGGGAAGTC
BrID90343 / TCGTCTTGCAACAATGACAAT / TTGATGGAAACTTTATTATATGCCT
BrID11773 / AGTTGTCGGCACTTATAGGA / CTTCTTCCACTCCATTTTTG
BrID120101 / ATTGCTCGTCTAAATCTTCG / GATATCGTGGGACACAAGTC
BrID11763 / CGGTTAAAGCTTATAGATCGC / GGCGGTGATCAAGAAAAA
BrID11765 / AATCTCCTGTCGGAAGGTAT / GTTGACCCTCTTAGGGTTTC
BrID90317 / TTCAAGACCCAACGAGAAGG / GGTGGTGGATACCTGCATCT
BrID11769 / CTCAATGGGATATCGAGTGT / AAGCAAGTGAGAGGTTTTGA
BrID120685 / CAACAGCATCTATGGAGTCTT / TGATCAGAGGTCAGGAGAAG
BrID130041 / TATTGTCGTGTGTGCAGTCT / TGTTGAGTTCTCGCAGTTTA
BrID11527 / CGCTTTACCAGTTTTTCTTC / AACTATCCGGAGACTAGGAA
SB13007 / TTGGATTTGGAGGAAAGACG / AACCATCATCAACGCAAACA
SB13031 / GATTCGACGAGTGAAGCCAT / ATAGAGAAAGGGGGCCTGAA
SB13037
SB14001 / GCCCTTCTTAAAGGGATTGC
TGTATGCATATTTCGCGGAT / GGCGGCTTTCAAAAGTAAGA
TGGGGACGACTTGTTGAGAT
SB13049 / AGTTGTCCTGAAATGACCCG / ACGCGGAGAGTTGTTTCACT
SB13023 / AGCATAAGCGATTCTGAGCC / GAGAAGATTCGATTTGCCGA
BrID130049 / CTGGTTCCAATTCAGTTCAT / GATGACCTAAACAAAGGCAA
BrID130051
GAA2 / TGCTGCTCTGTTAGGGTAGT
TCGGAAGAAGAATCATGTGG / ACATTTTGATTACCTCCCG
CTAATCCTCCAATCCCAGAC

Supplementary Table 2 The amplified primers of BrCRTISOcoding sequencesand promoter sequences

Primer / Location / Direction / Sequence (5′→3′)
Bio130094 / 5′-UTR / Forward / CCAACGGATAGGTGTAAC
Bio130137 / 3′-UTR / Reverse / AGACTATGCGAGTGTCCTTA
Bio130174 / CDS-Start / Forward / ATGAATCTCTGTCTCCACAATCCCG
Bio130175 / CDS-End / Reverse / CTATGCGAGTGTCCTTAACCAACCA
Bio130160 / Exon 8 / Forward / ATACAGATTGCCATCATTTC
Bio130127 / Exon 8 / Reverse / GATGGCAATCTGTATCTGG
Bio130253 / Exon 12 / Reverse / CCAATGTCAGCAGCTACACGATG
Bio130254
Bio130479
Bio130470
Bio130471
Bio130480
Bio140286
Bio140287 / Exon 12
894 -bp
Exon 1
1292 -bp
Exon 1
812 -bp
333 -bp / Forward
Forward
Reverse
Forward
Reverse
Forward
Reverse / TAGATGGTTTGTACTGCGTTGG
ACGGTCTCAACATACTCGTTCAAG
ACGGGATTGTGGAGACAGAGATT
CCAAGTAGAAGAAGAATCAG
GAGGAGCTGCGATCAGCACA
TTGGGCCATTTGATTTATTC
CCTTCCTGTAGCCTTTGTTT

Note: “-”means the upstream of the candidate gene BrCRTISO.

Supplementary Table 3 Identification of carotenoids that accumulated in the inner leaves of Chinese cabbage

NO. / Compound / Retention Time /min / Spectral characteristics/nm / III/II % b
Observed / Reported / Observed / Reported
1 / Violaxanthin / 6.73 / 416,441,469 / 416,440,465 / 97 / 98
2 / Luteina / 10.55
3 / Zeaxanthina / 11.62
4 / Prolycopene / 35.74 / 423,442,464 / 424,442,464 / 10 / 10
5 / Proneurosporene / 36.70 / 413,434,462 / 412,434,462 / 10 / 10
6 / Pro-ζ-carotene / 37.74 / 380,402,427 / 380,404,428 / 107 / 107
7 / ζ-carotene / 39.47 / 381,402,427 / 378,400,425 / 98 / 103
8 / 9-cis-β-carotene / 40.97 / 423,447,469 / 422,446,470 / 18 / 18
9 / β-carotenea / 42.03
10 / Neurosporene / 43.95 / 414,439,468 / 414,439,467 / 100 / 100

Note: a The compound was identified by standards, so spectral characteristics were not shown; b Ratio of the height of the longest-wavelength absorption peak, designated III, and that of the middle absorption peak, designated II, taking the minimum between two peaks as baseline, multiplied by 100 (Britton 1995).

Supplementary Table 4 Cis-acting regulatory element analysis of the promoter sequences in mutational region

No. / Site Name / Position / sequence / function
1 / Box I / 371 / TTTCAAA / light responsive element
2 / CAAT-box / 399 / CAAAT / common cis-acting element in promoter and enhancer regions
3 / CAAT-box / 308 / CAAT / common cis-acting element in promoter and enhancer regions
4 / CAAT-box / 368 / CAATT / common cis-acting element in promoter and enhancer regions
5 / CAAT-box / 353 / CAAAT / common cis-acting element in promoter and enhancer regions
6 / CAAT-box / 370 / CAAAT / common cis-acting element in promoter and enhancer regions
7 / GCN4_motif / 494 / TGAGTCA / cis-regulatory element involved in endosperm expression
8 / HSE / 353 / AAAAAATTTC / cis-acting element involved in heat stress responsiveness
9 / Skn-1_motif / 493 / GTCAT / cis-acting regulatory element required for endosperm expression
10 / Sp1 / 469 / CC(G/A)CCC / light responsive element
11 / TATA-box / 296 / TATAA / core promoter element around -30 of transcription start
12 / TATA-box / 297 / TATA / core promoter element around -30 of transcription start
13 / TATA-box / 336 / TTTTA / core promoter element around -30 of transcription start
14 / TATA-box / 345 / TTTTA / core promoter element around -30 of transcription start
15 / TATA-box / 358 / TTTAAAAA / core promoter element around -30 of transcription start
16 / TATA-box / 359 / TTTTA / core promoter element around -30 of transcription start
17 / TATA-box / 362 / TTTTA / core promoter element around -30 of transcription start
18 / TATA-box / 420 / TATA / core promoter element around -30 of transcription start
19 / TATA-box / 428 / TTTAAAAA / core promoter element around -30 of transcription start
20 / TATA-box / 429 / TTTTA / core promoter element around -30 of transcription start
21 / TATA-box / 432 / TTTTA / core promoter element around -30 of transcription start
22 / TATA-box / 444 / TAATA / core promoter element around -30 of transcription start

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