Supplementary Fig. S2. Multiple Alignments of Α-, Β-, and Γ-Type Cas

Supplementary Fig. S2. Multiple alignments of α-, β-, and γ-type CAs.

α-type CAs

Streptococcus_thermophilus_CNRZ1 ----MNKKSFKLIGLLSLIAATILLVACQSDTKTEASKPKD------VQWGYTGSTGPDHWGDLSKDYELSKNGKEQSPI

Streptococcus_thermophilus_LMG18 ----MNKKSFKLIGLVSLVASTILLVACHSDTKTEASKPKD------VQWGYTGSTGPDHWGDLSKDYELSKNGKEQSPI

Streptococcus_thermophilus_LMD9_ ----MNKKSFKLIGLLSLIAATILLVACQSETKSKTSKSQE------VQWGYTGITGPEHWGDLSKDYELSKNGKEQSPI

Streptococcus_mutans_SMU.1595 ----MKKK---FIYLTVLVVVAVFCISTFLTLKGD-SQDRE------AHWSYSGSTSPEHWGDLSDDYKAAKDGKEQSPI

Listeria_welshimeri_SLCC5334_lwe ------MTKEK------VLWGYDEKTGPEMWGHICSDFEIAHTGKAQSPV

Listeria_monocytogenes_F2365_LMO ------MTKEK------VLWGYDEKTGPEMWGHICSDFEIAHTGKAQSPV

Listeria_innocua_lin0807 ------MTKEK------VLWGYDEKTGPEMWGHICSDFEIAHTGKAQSPV

Listeria_monocytogenes_EGDe_lmo0 ------MTKEK------VLWGYDEKTGPEMWGHICSDFEIAHTGKAQSPV

Bacillus_halodurans_BH0360 MKKYLWGKTCLVVSLSVMVTACSSAPSTEPVDEPSETHEETSGGAHEVHWSYTGDTGPEHWAELDSEYGACAQGEEQSPI

Bacillus_clausii_ABC3182 MKRSHLFTSITLASVVTLATAP--AASAASFLSPLQALKAS------WSYEGETGPEFWGDLDEAFAACSNGKEQSPI

Lactobacillus_casei_LSEI_0650 ------MSILNYAKQSSWPNGFGFQQSPI

Lactobacillus_brevis_LVIS_2247 ------MSILNYAEQDSWSNGFGSQQSPI

Lactobacillus_plantarum_lp_2736 ------MTFDYAHQTDWPMTSGQHQSPI

Oenococcus_oeni_OEOE_0238 ------MPKLDYDNQNNWLFKTGQMQSPV

Lactobacillus_delbrueckii_ATCC_B ------MEYINYQTQDQWQG-PASQQSPI

Lactobacillus_delbrueckii_ATCC_1 ------MEYINYQTQDQWQG-PASQQSPI

Enterococcus_faecalis_EF1711 ------MKKIKNMD------VEWSYTGNDGPEHWHTLCDWFAEGAKFAYQSPI

Pediococcus_pentosaceus_PEPE_065 ------MRKLKYTQQDQWESEARETQSPI

Synechococcus_elongatus_PCC7942_ ------MRRRSLLAALGGSCIGWLGSSQPVWAS------ADWDYSRRRGPRQWAKLDPAYAICQQGRQQSPI

Streptococcus_thermophilus_CNRZ1 NITGAEDVDLP--ELNLNNQESEAQVENNGHTIEVSFKN-PKNTLTIGKEVYKLQQFHFHAPAENEIDG--KTYPLEGHF

Streptococcus_thermophilus_LMG18 NITGAEDVDLP--ELNLNNQESEAQVENNGHTIEVSFKN-PKNTLTIGKEVYKLQQFHFHAPAENEIDG--KTYPLEGHF

Streptococcus_thermophilus_LMD9_ NITGAEDVDLP--ELNLNNQESEAQVENNGHTIEVSFKN-PKNTLTIGKEVYKLQQFHFHAPAENEIDG--KTYPLEGHF

Streptococcus_mutans_SMU.1595 NITGAKDVDLP--SLQLNSKNSKAEVENNGHTIEVKLKN-KKNTLKFNGKTYTLEQFHFHAPSENQIDG--KTYPLEGHF

Listeria_welshimeri_SLCC5334_lwe NIEQADVVKLKPSTMKFYYKETDYTIRRIEQSVHVFPHD-KEQGLRFNGEYYPLVSFHAHIPAEHLLDG--YIYPIEWHF

Listeria_monocytogenes_F2365_LMO DIEQADVVKLKPSTMKFYYKETDYTIRRIEQSVHVFPHD-KEQGLRFNGEYYPLVSFHAHIPAEHLLDG--YVYPIEWHF

Listeria_innocua_lin0807 NIEQADVVKLKPSTMKFYYKETDYTIKRIEQSVHVFPHD-KEQGLRFNGEYYPLVSFHAHIPAEHLLDG--YVYPIEWHF

Listeria_monocytogenes_EGDe_lmo0 DIEQADVVKLKPSTMKFYYKETDYTIRRIEQSVHVFPHD-KEQGLRFNGEYYPLVSFHAHIPAEHLLDG--YIYPIEWHF

Bacillus_halodurans_BH0360 NLDKAEAVDTD-TEIQVHYEPSAFTIKNNGHTIQAETTS-DGNTIEIDGKEYTLVQFHFHIPSEHEMEG--KNLDMELHF

Bacillus_clausii_ABC3182 NLFYDREQTSK-WNWAFSYSEAAFSVENNGHTIQANVENEDAGGLEINGEAYQLIQFHFHTPSEHTIEE--TSFPMELHL

Lactobacillus_casei_LSEI_0650 DLR--QATTTVDTDMTILTPWMTDQEID---DQVTIRLA-GQGTTRINETTWAFVQAHFHVPAEHIVA---EKTVAELHF

Lactobacillus_brevis_LVIS_2247 DLR--QAEPTSVTNFVVTDPWIADREID---DQVTIQLA-GHGTTRINEMTWHFVQAHIHVPAEHITT---EETVAELHF

Lactobacillus_plantarum_lp_2736 DIQTQHVQSSQLSAINWRGLYQAKTITG---EATTLKAS-GIGAAYLNDRDFAFQQIHFHTPAEHLIDG--QAAPIEWHL

Oenococcus_oeni_OEOE_0238 ALESNKAEKIEKQATLKLNYSTNASYVHD--NGQGIEIG-LSGKAIIDNRFFSLQQFHIHAPSEHLLN--NYRFDGEIHF

Lactobacillus_delbrueckii_ATCC_B DIVLEQTTPRPLADQPLTVSFVGDQALTRDPRSSGDQFI-GAGTLTLGNHHYHFLRLHFHDHSEHLFDG--QRQDAEVHF

Lactobacillus_delbrueckii_ATCC_1 DIVLEQTTPRPLADQPLTVSFVGDQALTRDPRSSGDQFI-GAGTLTLGNHHYHFLRLHFHDHSEHLFDG--QRQDAEVHF

Enterococcus_faecalis_EF1711 ALEKESAETVN-SQITFHYKKEEFTEKEFKNTFHFVPPN-TESYVMFENVAYHLTDIHFHMPSEHLLSG--KQYPLEFHL

Pediococcus_pentosaceus_PEPE_065 LIETAKLKAKKNQIVDGLKNYT-ATELHK--NYNSLSLT-ANGKMEADQRSFQLEEVHFHHPAEHCFKGEATPRVLEAHF

Synechococcus_elongatus_PCC7942_ NLTG----QPDRTPLDYRDRPFKGILQQAPHSLRIDCPA--GNGFWEAGTFYELLQFHFHTPSEHQHQG--QRFPAEIHF

Streptococcus_thermophilus_CNRZ1 VYKTDNGKITVVSVLYNYGDKN----QALQLIWDKMPQAANTET-DLSQAISLDDFYPENKDYYNFEGSLTTPPCTEGVN

Streptococcus_thermophilus_LMG18 VYKTDNGKITVVSVLYNYGDKN----QALQLIWDKMPQAANTET-DLSQAISPDDFYPENKDYYNFEGSLTTPPCTEGVN

Streptococcus_thermophilus_LMD9_ VYKTDNGKITVVSVLYNYGDKN----QALQLIWDKMPQAANTET-DLSQAISPDDFYPENKDYYNFEGSLTTPPCTEGVN

Streptococcus_mutans_SMU.1595 VYTTKDKKITVISILYQYGKEN----KTLKQVWKKMPQKAETKK-NLSQPVAISQLFPKDLDYYNFEGSLTTPPCTEGVN

Listeria_welshimeri_SLCC5334_lwe VHEKPDGTTLVMSAWMEIDNTNNVEFKDLPTYFPEVFADFETER-EITLDVN--EFMPEERVFYTYQGSRTTPPTVEGVT

Listeria_monocytogenes_F2365_LMO VHEKPDGTTLVMSAWMEIDNTNNVEFKDLPTYFPEVFADFETER-EITLDVN--EFMPEERVFYTYQGSRTTPPTVEGVT

Listeria_innocua_lin0807 VHEKPDGTTLVMSAWMEIDNTNNVEFKDLPTYFPEVFADFETER-EITLDVN--EFMPEERVFYTYQGSRTTPPTVEGVT

Listeria_monocytogenes_EGDe_lmo0 VHEKPDGTTLVMSAWMDIDNTNNIEFKNLPTYFPEVFADFETER-EITLDVN--EFMPEERVFYTYQGSRTTPPTVEGVT

Bacillus_halodurans_BH0360 VHKNENDELAVLGVLMKAGEEN----EELAKLWSKLPAEETEENISLDESIDLNALLPESKEGFHYNGSLTTPPCSEGVK

Bacillus_clausii_ABC3182 VHANHAGDLAVLGVLMEMGNDH----EGIEAVWEVMPEEEGTAAYSI--SLDPNLFLPESVTAYQYDGSLTTPPCSEGVK

Lactobacillus_casei_LSEI_0650 VHQSAIGALCVVAVLVPVGQAN------PIMADVLDHFQPHVTQPIN--FDLTRLLPKQGTVYQYLGSLTTPPLTEGVT

Lactobacillus_brevis_LVIS_2247 VHQSVIGALCVVAVLVPIGKAN------PIIDGILDHFQPHATESID--CDLTGLLPNRGTVFQYLGSLTTPPLTEGVT

Lactobacillus_plantarum_lp_2736 VHQSATGQLAVVAVFGRIGKPN------SSLQGLLDQFTAQTSHELTTPVNLTELLPDTGTVYHYLGSLTTPPLSETVE

Oenococcus_oeni_OEOE_0238 VHQAKDGRTAVIAVFLKAGKTS------KTFSQIFNHLNQD-QNFVC---DLTDLIPENKSYYHYLGSLTTPPLTENVE

Lactobacillus_delbrueckii_ATCC_B VYQDDQGQTLVLAMLG-IRAAD------GEAG---LDFAPVINGQAG-AAYLNQFFANNQGYFNYTGSLTTPPLSPDVT

Lactobacillus_delbrueckii_ATCC_1 VYQDDQGQTLVLAMLG-IRAAD------GEAG---FDFAPVINGQAG-SAYLNQFFANNQGYFNYTGSLTTPPLSPDVT

Enterococcus_faecalis_EF1711 VHMNDAGENLVVGCLFTITEEE------NRFSEANHPMDWENGTHQQWFNPSIFLPEERLHYHYVGSLTTPPTKGPVK

Pediococcus_pentosaceus_PEPE_065 VHYGCLRQPLVVSVTFKLGAKN------ATFEKILTAMDDNKVEQFI---LLDDLIPKRGYFYRYIGSLTTPPLTEGVE

Synechococcus_elongatus_PCC7942_ VHRSDRDQLAVVGVFLAAGDRP------LPILDTLLAVPPSTDNQLLSTAIQPTDLMPRDRTVWRYSGSLTTPPCSEPVL

Streptococcus_thermophilus_CNRZ1 ------

Streptococcus_thermophilus_LMG18 WIVFKSQETVSKEQVEKFSQTLGFENNRPIQDANGRKIKA------

Streptococcus_thermophilus_LMD9_ WIVFKSQETVSKEQVEKFSQTLGFENNRPIQDANGRKIKA------

Streptococcus_mutans_SMU.1595 WIVFKKQENISKAQVKKFSKTLGFNNNRPIQKLNGREIKE------

Listeria_welshimeri_SLCC5334_lwe WIVLKNAKTLGQEDFTEFEKAIG-NTSRPVQDLNGREITFYN------

Listeria_monocytogenes_F2365_LMO WIVLKNAKTLGQEDFTEFEKAIG-NTSRPVQDLNGREITFYN------

Listeria_innocua_lin0807 WIVLKNAKTLGQEDFTEFEKAIG-NTSRPVQDLNGREITFYN------

Listeria_monocytogenes_EGDe_lmo0 WIVLKNAKTLGQEDFTEFEKAIG-NTSRPVQDLNGREITFYN------

Bacillus_halodurans_BH0360 WTVLSEPITVSQEQIDAFAEIFP-DNHRPVQPWNDRDVYDVITE------

Bacillus_clausii_ABC3182 WTVLNDTISISETQLDAFRDIYP-QNYRPVQELGDREIGFHYH------

Lactobacillus_casei_LSEI_0650 WYVIEQTEITMSSNQLARYQQFFEPNNRHVQAINRRPIFRGVFKQLKRSAGDDPLHG

Lactobacillus_brevis_LVIS_2247 WYVIEQTDITISSEQLKRYKQLFGPNNRQVQAANQRPVLRGSGTFTK------

Lactobacillus_plantarum_lp_2736 WYVCADT-VMIGEQQLAAYQQLFAANNRKIQPLNDRPIIAERF------

Oenococcus_oeni_OEOE_0238 WYILANPVEISRQQLAEFHKLYP-YNNRQLQSLNGRPVLYCSSTIKN------

Lactobacillus_delbrueckii_ATCC_B WVVLDAVHPFSSGS-LALIHDLFADNYRAPQPITVPVYHYYESGK------

Lactobacillus_delbrueckii_ATCC_1 WVVLDAVHPFSSGS-LALIHDLFADNYRAPQPITVPVYHYYESGK------

Enterococcus_faecalis_EF1711 WFVFDTIQKMDQAFLNKIKEGMLAFNNRPLQPLNGRKIYFSND------

Pediococcus_pentosaceus_PEPE_065 WVVFEQAQMIDIDQLNLYMATFKEQNNRELQKLNHRKIEKYEI------

Synechococcus_elongatus_PCC7942_ WRVCDRPLFVARQQLRQLRQRLG-MNARPLQA------

β-type CAs

Bacillus_anthracis_Ames_0581_GBA ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_anthracis_Ames_BA5049 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_anthracis_A2012_BA_5466 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_anthracis_Sterne_BAS468 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_cereus_ZK_BCZK4546 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_thuringiensis_9727_BT97 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_thuringiensis_Al_Hakam_ ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_cereus_ATCC_10987_BCE_4 ------MKS------LEEILQYNEKFVEE------KKYEEFVAGKFPNKKMVIIS

Bacillus_cereus_ATCC_14579_BC479 ------MKS------LEEILQYNEKFVEE------KKYEEYETGKFPNKKMVIIS

Bacillus_cereus_cytotoxis_NVH_39 ------MKS------LEEILRYNEKFVEE------KKYEEYEAGKFPNKKMVIVS

Bacillus_licheniformis_DSM13_BLi ------MKL------LDNILQFNQQFVER------QDYQKYQTSKFPDKRMVILS

Bacillus_licheniformis_ATCC_1458 ------MKL------LDNILQFNQQFVER------QDYQKYQTSKFPDKRMVILS

Bacillus_subtilis_BG13863 ------MSL------LNDILEFNKTFTEQ------REYEKYQTSKFPDKKMAILS

Bacillus_amyloliquefaciens_RBAM_ ------MSL------LNDIMQFNEQFTKN------KDYEKYQTSKFPDKKMAILS

Bacillus_pumilus_BPUM_2707 ------MKL------LEEIIEYNQQFIEE------KKYEEFTTTKFPQKKAVILS

Bacillus_halodurans_BH3286 ------MSL------LGSILTYNQSFVEK------KAYKPFQTSKFPDQKMVVLT

Bacillus_amyloliquefaciens_RBAM_ ------MVS------LSSILEHNQQFVQE------RKYEPYQTTKFPKKKLVIVS

Bacillus_subtilis_BG12410 ------MNQMVS------LTSILEHNQRFVSE------KKYEPYKTTKFPSKKLVIVT

Bacillus_licheniformis_DSM13_BLi ------MAI------LTSILKHNQSFVEE------KGYEFYETTKFPEKKLVILT

Bacillus_licheniformis_ATCC_1458 ------MAI------LTSILKHNQSFVEE------KGYEFYETTKFPEKKLVILT

Bacillus_pumilus_BPUM_3106 MRTHERKGGQAGAYVTYKEMGDGKMGSK------LEQILQHNSEFVHE------RHYEPYKAGKFPEKKLVILT

Bacillus_clausii_ABC2875 ------MIQMSK------LESILAFNEAFVAN------KEYERFKADKFPQKKIVILT

Clostridium_perfringens_ATCC_131 ------MEKNVRR------LEEILEFNKSFVEN------KEYEKYKATKKPEKKLVILS

Clostridium_perfringens_13_CPE04 ------MEKNVRR------LEEILEFNKSFVEN------KEYEKYKATKKPEKKLVILS

Clostridium_perfringens_SM101_CP ------MRR------LEEILDFNKNFVKN------KEYEKYKATKKPEKKLVILS

Clostridium_beijerinckii_Cbei_44 ------MSK------LEEIIKYNESFVGN------KDYQEYVTTKIPKKKMAILS

Clostridium_difficile_CD2213 --MYILYFNSDIIIKKFLKRVILHMRK------LEEILEYNKSFVEN------KEYEQYVTSKHPNKKIVVLS

Clostridium_kluyveri_CKL_0534 ------MNK------LQQILDYNRCFVEN------KRYDKYTTSKHPDKKLVILS

Desulfotomaculum_reducens_Dred_2 ------MSR------FEEIIAFNQNFVDQ------KEFEKYQTTKYPDKKMVILT

Clostridium_phytofermentans_Cphy ------M------IEEILEFNKAFVEN------KGYEKYITNKFPQKKVAIVS

Oceanobacillus_iheyensis_OB1097 ------MH------LDHMLEFNKNFVEN------KSYESYSTNTYPNKRMVVFT

Pelotomaculum_thermopropionicum_ ------MKKENPACQRPGAVTGEEARRLLMEGNGRFVEGRLAPKDLGGARREELASKGQKP--FAVIVT

Clostridium_acetobutylicum_CAC24 ------MKDEFLVIEKT------LMEGNKRFMSNKLKLKDFSEKRRKDLKENGQRP--MAVVVS

Desulfitobacterium_hafniense_DSY ------MCAGRVMVTEK------LLKGIVNFRNGDFET----HRQLFEELKDNQKP--HTLFIT

Syntrophomonas_wolfei_Swol_2452 ------MSHHATIEVDK------AALFKSLEQGILAFEKGFPLEDFKKGNYGQHP--LITLLT

Synechococcus_elongatus_PCC7942_ ------MRK------LIEGLRHFRTSYYPS----HRDLFEQFAKGQHP--RVLFIT

Bacillus_anthracis_Ames_0581_GBA CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_anthracis_Ames_BA5049 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_anthracis_A2012_BA_5466 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_anthracis_Sterne_BAS468 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_cereus_ZK_BCZK4546 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_thuringiensis_9727_BT97 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_thuringiensis_Al_Hakam_ CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_cereus_ATCC_10987_BCE_4 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_cereus_ATCC_14579_BC479 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCVVGHHDCG--MAKIQASSTI

Bacillus_cereus_cytotoxis_NVH_39 CMDTRLVELLPKAMNMRNGDVKIIKVAGAVISHPFG-----SIMRSILVAVYELGADEVCIIGHHDCG--MAKIQASSTI

Bacillus_licheniformis_DSM13_BLi CMDTRLVELLPHAMNMRNGDVKIVKSAGALVAHPFG-----SIMRSILVAVYELNADEVCVIGHYDCG--MSKLSCDSFL

Bacillus_licheniformis_ATCC_1458 CMDTRLVELLPHAMNMRNGDVKIVKSAGALVAHPFG-----SIMRSILVAVYELNADEVCVIGHYDCG--MSKLSCDSFL

Bacillus_subtilis_BG13863 CMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFG-----SIMRSILVAVYELNADEVCVIGHHDCG--MSKISSKSML

Bacillus_amyloliquefaciens_RBAM_ CMDTRLVELLPHAMNMRNGDVKIIKSAGALVAHPFG-----SIMRSLLVAVYELNADEVCVIGHHDCG--MSKINSEDML

Bacillus_pumilus_BPUM_2707 CMDTRLVELLPRAMNMKNGDIKIVKSAGALVSHPFG-----SIMRSILVAVYELNADEVYVIGHHDCG--MSKIDSQTLL

Bacillus_halodurans_BH3286 CMDTRLSQLLPHAMNIKNGDAKIIKNAGAVITHPYG-----SIMRSIIVALYELKAKEVYIVGHHGCG--MAGLQAESVL

Bacillus_amyloliquefaciens_RBAM_ CMDTRLTELLPQAMGLRNGDAKIIKNAGAIVSHPFG-----SVMRSILVAVYELQAEEVCIIGHRECG--MSGLNAASIL

Bacillus_subtilis_BG12410 CMDTRLTELLPQAMGLKNGDAKIVKNAGAIVSHPFG-----SVMRSILVAIYELQAEEVCIVGHHECG--MSGLNASSIL

Bacillus_licheniformis_DSM13_BLi CMDTRLLELLHHAMGLKNGDAKIIKNAGAVVSHPFG-----SVMRSILVAVYELQAEEVCVIGHHECG--MASLNASSIL

Bacillus_licheniformis_ATCC_1458 CMDTRLLELLHHAMGLKNGDAKIIKNAGAVVSHPFG-----SVMRSILVAVYELQAEEVCVIGHHECG--MASLNASSIL

Bacillus_pumilus_BPUM_3106 CMDTRLLELLPQSMGLRNGDAKVIKNAGAIVTHPFG-----SVMRSILLAIYELKAEEVCIVGHHECG--MAGLAADPLL

Bacillus_clausii_ABC2875 CMDTRLVELLHNAMNLKNGDAKIIRNAGAVISHPFG-----SIMRSILVAIYELGAEEVFVIGHYGCG--MTGLSANSVL

Clostridium_perfringens_ATCC_131 CMDTRLTELLPKAMNIKNGDAKIIKNAGATIMHPFG-----SIVRSILVAIYEFNAEDVLVVGHHGCG--MSNLNSKDMI

Clostridium_perfringens_13_CPE04 CMDTRLTELLPKAMNIKNGDAKIIKNAGATIMHPFG-----SIVRSILVAIYEFNAEDVLVVGHHGCG--MSNLNSKDMI

Clostridium_perfringens_SM101_CP CMDTRLTELLPKAMNIKNGDAKIIKNAGATIMHPFG-----SIVRSILVAIYEFNAEDVLIVGHHGCG--MSNLNSKDMI

Clostridium_beijerinckii_Cbei_44 CMDTRLTELLPKAMNIKNGDAKIIKDAGATVMHPFG-----GVMRSILVAVYEFGAEDVFVIGHHGCG--MSNLNTKKLV

Clostridium_difficile_CD2213 CMDTRLTELLPKAMNLKNGDVKLIKNAGATIMHPFG-----SIMRSVLVAIYEFDVDEVMVVGHHGCG--MCNVDTDKLL

Clostridium_kluyveri_CKL_0534 CMDTRLTELLPKALNFKNGDVKLIKNAGASVMHPFG-----SIMRSIVVAVYEFQADEVLVIGHHDCG--MSNLNSDKIL

Desulfotomaculum_reducens_Dred_2 CMDTRLTELLPKAMNLKNGDAKIIKNAGGLVSHPLG-----SIMRSILVAIYELGAEEVFVIGHYDCG--MANINPHRTL

Clostridium_phytofermentans_Cphy CMDTRLTELLPASMGLKNGDAKIIKNAGGVISHPFG-----SAMRSLLIAIYELSVSEILVIGHTDCG--ARYTDSQKMI

Oceanobacillus_iheyensis_OB1097 CMDSRLLELLPKALDFKNGDVKMVRNAGAIIRDPFD-----SAMKSLLVAIYKLQADEVVIIGHHDCG--MSHVDTKELK

Pelotomaculum_thermopropionicum_ CSDSRVPPEILFDQAL--GDLFVIRVAGNVVDQV------ALGSVEYAVEHLNTPLVVVMGHEKCGAVQAAVDGGEVP

Clostridium_acetobutylicum_CAC24 CSDSRVPPEIIFDLGL--GEIFTVRNAGNIVDSN------TIGNVEFAVNHLGAKYVLVMGHEKCGAVEAALEGVSNN

Desulfitobacterium_hafniense_DSY CSDSRIDPNMITGTLP--GELFIVRNVANIVPPCRETSEYVSTTSAIEYAVQMLGVENIIVCGHSNCGGCSASLNAPEKL

Syntrophomonas_wolfei_Swol_2452 CADSRMPVNIFGDIFN---RIFSVENIGNQVKTNEG------SVLYGLLHLHTPLMIVAGHSDCG--AIKAAESNFV

Synechococcus_elongatus_PCC7942_ CSDSRIDPNLITQSGM--GELFVIRNAGNLIPPFGAANG--GEGASIEYAIAALNIEHVVVCGHSHCGAMKGLLKLNQLQ

Bacillus_anthracis_Ames_0581_GBA EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_anthracis_Ames_BA5049 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_anthracis_A2012_BA_5466 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_anthracis_Sterne_BAS468 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_cereus_ZK_BCZK4546 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_thuringiensis_9727_BT97 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_thuringiensis_Al_Hakam_ EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_cereus_ATCC_10987_BCE_4 EKMKE----RGITEEKLDMLR---YSGIDLEQFLRGFSSVEESVEHSVSVLRNHP-----LLPEEVPVHGLVIDPD----

Bacillus_cereus_ATCC_14579_BC479 EKMKE----RGITNEKLDTLR---YSGIDLERFLQGFSSVEESVEHSVSILRNHP-----LLPEEVPVHGLVIDPE----

Bacillus_cereus_cytotoxis_NVH_39 EKMKE----RGVSEERLDALR---YSGIDLEKFLQGFSSVEESVEHSVSVLRNHP-----LLPTEVPVHGLVIHPD----

Bacillus_licheniformis_DSM13_BLi DKVVK----RGIPKERIETLE---YSGVDFEQWLKSFDSVEDSVRDSVSVIRNHP-----LMPEEVPVHGLVIDPE----

Bacillus_licheniformis_ATCC_1458 DKVVK----RGIPKERIETLE---YSGVDFEQWLKSFDSVEDSVRDSVSVIRNHP-----LMPEEVPVHGLVIDPE----

Bacillus_subtilis_BG13863 EKIKA----RGIPEERIETIK---YSGVDFDQWFKSFDSVEASVKDSVDVIKHHP-----LFPENVPVHGLVIDPK----

Bacillus_amyloliquefaciens_RBAM_ DKIID----RGIPKERIDMLK---HSGVDLDQWLKSFDSVEASVTDSVNVIKHHP-----LFPDNVPVHGLVIDPE----

Bacillus_pumilus_BPUM_2707 NKAIE----RGIPEKRIEVLE---YSGIDFKQWLKSFSSVEESVKDSVSVVKNHP-----LLPSNVPVHGLVIDPG----

Bacillus_halodurans_BH3286 EKAKK----HGVSEEALERLL---AQGIDVNQFLSGFDKVEDSVKHSANVVRTHP-----LIPHDVPVHALVICPK----

Bacillus_amyloliquefaciens_RBAM_ NKAKE----RGVEDKCLSLLQ---HAGVDLETWLTGFHSVEESVAHSVNMVRHHP-----LLPEEVPVHGLVIDPG----

Bacillus_subtilis_BG12410 EKAKE----RGVEDSCLNLLT---SAGLDLKTWLTGFHSVEESVSHSVNMIKNHP-----LLPKKVPVHGLVIHPE----

Bacillus_licheniformis_DSM13_BLi QKAKQ----RGVDDGCLELLQ---HSGIDLDTWMTGFDSVEDSVSHSVNMIRKHP-----LLSSDVPVHGLVIDPK----

Bacillus_licheniformis_ATCC_1458 QKAKQ----RGVDDGCLELLQ---HSGIDLDTWMTGFDSVEDSVSHSVNMIRKHP-----LLSSDVPVHGLVIDPK----

Bacillus_pumilus_BPUM_3106 EKAKA----RGIEEKCLSIVK---NSGVDLKGWLTGFDSVEESVSQSVKLVKEHP-----LMPNDVAVHGLVIDPA----

Bacillus_clausii_ABC2875 QKAEE----RGIDMDEINALQ---YAGVDVNKFLTGFENVTESVNHSVDMVINHP-----LLPKDVRVHGLVIDPE----

Clostridium_perfringens_ATCC_131 SKMED----RGISEETILTIK---HCGIDVEKWLHGFGCVEESVKESVTSLKNHP-----LMPSDVNVHGLVIDPH----

Clostridium_perfringens_13_CPE04 SKMED----RGISEETILTIK---HCGIDVEKWLHGFGCVEESVKESVTSLKNHP-----LMPSDVNVHGLVIDPH----

Clostridium_perfringens_SM101_CP KKMES----RGISEETILTIK---HCGIDVEKWLHGFGCVEESVKKSVTSLKNHP-----LMPSDVNVHGLVIDPQ----

Clostridium_beijerinckii_Cbei_44 DKMLD----RGIKEETLSTLN---NAGVNVERWLHGFESVEESIKESVKMIKDHP-----LLPSDVRVHGLIMSPE----

Clostridium_difficile_CD2213 GKILD----RGISNDVILTLR---SAGIDTKQWLHGFDSAEESVKDSIDLIKGHP-----LIPDGIIVHGLIISPE----

Clostridium_kluyveri_CKL_0534 KQAIK----RGIASDVIITLS---DAGIDIKKWLQGFNSVEESVKESVSLIRKHP-----LIPRDIKIHGLIMDPE----

Desulfotomaculum_reducens_Dred_2 ENMVQ----RGISKETITTFE---YAGLNIERWLHGFDNVTDSVRNSVQIIKKHP-----LMPPGILVHGFVIDPE----

Clostridium_phytofermentans_Cphy EKMKG----QGITQNNIDMMK---YCGIDFDSWLGGFVDLDESIKKSVELIRNHP-----LIPETVMVYGLIIDSE----

Oceanobacillus_iheyensis_OB1097 NEMIE----RGINKESIDVLA---NGGVDFDKEFQGFETVEESVSQGVNLVRNHP-----LLPEEIKVHGLVIDPG----

Pelotomaculum_thermopropionicum_ GSIG---AIVSKIRPSVQKAA---AAG--AAGGELYEKTADENIKSAIEELKKSPVIRHFMEEGRLMLIGAKYHLS----

Clostridium_acetobutylicum_CAC24 EKLK---GFIEPLENVVANAL---ENNKKCTRKEIIDIIEDKNVEASANKLLESENLRQLKRKDEIEIVKAKYFHE----

Desulfitobacterium_hafniense_DSY DQLPHTKKWLELMESVRERVL---SEFAEDEPTVREWMMEQINVVEQLRHLMTYPYIYEKVMAKELMLSGWHYMIE----

Syntrophomonas_wolfei_Swol_2452 DEPMGIRNELSIVKNSLEEARRKSGLSFDDEPGLKFSKLAEVNVDRQIDYLLANYAVADLVEKNELLLLGVMMDLHNIYG

Synechococcus_elongatus_PCC7942_ EDMPLVYDWLQHAQATRRLVL---DNYSGYETDDLVEILVAENVLTQIENLKTYPIVRSRLFQGKLQIFGWIYEVE----

Bacillus_anthracis_Ames_0581_GBA TGKLDLVVNGYDN------

Bacillus_anthracis_Ames_BA5049 TGKLDLVVNGYDN------

Bacillus_anthracis_A2012_BA_5466 TGKLDLVVNGYDN------

Bacillus_anthracis_Sterne_BAS468 TGKLDLVVNGYDN------

Bacillus_cereus_ZK_BCZK4546 TGKLDLVVNGYDN------

Bacillus_thuringiensis_9727_BT97 TGKLDLVVNGYDN------

Bacillus_thuringiensis_Al_Hakam_ TGKLDLVVNGYDN------

Bacillus_cereus_ATCC_10987_BCE_4 TGKLDLVVNGYEN------

Bacillus_cereus_ATCC_14579_BC479 TGKLDLVVNGYDN------

Bacillus_cereus_cytotoxis_NVH_39 TGKLDLVVNGYEA------

Bacillus_licheniformis_DSM13_BLi TGRLDLIVNGYPEER------

Bacillus_licheniformis_ATCC_1458 TGRLDLIVNGYPEER------

Bacillus_subtilis_BG13863 TGKLDLIVNGYNN------

Bacillus_amyloliquefaciens_RBAM_ TGKLDLVIDGYQHMD------

Bacillus_pumilus_BPUM_2707 TGKLDVVVNGYEK------

Bacillus_halodurans_BH3286 TGKLDVVENGYDIVQAAKPSQQ------

Bacillus_amyloliquefaciens_RBAM_ TGTLDVVVDGYETVKGGCS------

Bacillus_subtilis_BG12410 TGKLDVVINGYETELINNHS------

Bacillus_licheniformis_DSM13_BLi TGRLDVVEDGYSREDVSLSEVR------

Bacillus_licheniformis_ATCC_1458 TGRLDVVEDGYSREDVSLSEVR------

Bacillus_pumilus_BPUM_3106 TGKLDVVVKDKQIDAQYT------

Bacillus_clausii_ABC2875 TGKLDLLQRDLQKGANAKEPQDA------

Clostridium_perfringens_ATCC_131 TGELKVIVNGYE------

Clostridium_perfringens_13_CPE04 TGELKVIVNGYE------

Clostridium_perfringens_SM101_CP TGELNVIVNGYE------

Clostridium_beijerinckii_Cbei_44 TGELEIIIDGDIKDAN------

Clostridium_difficile_CD2213 TGKLDVVVNGYEDK------

Clostridium_kluyveri_CKL_0534 TGKLEVIVNGY------

Desulfotomaculum_reducens_Dred_2 TGKLDVVVHGR------

Clostridium_phytofermentans_Cphy TGELSRIV------

Oceanobacillus_iheyensis_OB1097 SGKVDVVTRDERK------

Pelotomaculum_thermopropionicum_ SGRVVFYH------

Clostridium_acetobutylicum_CAC24 TGEVKILR------

Desulfitobacterium_hafniense_DSY TGEVFIYDYRAG------EFRLANGSRA------

Syntrophomonas_wolfei_Swol_2452 NGYGMLYTSNINGERNSSILKSYSHLGLLADRARRLTSY------

Synechococcus_elongatus_PCC7942_ SGEVLQISRTSSDDTGIDECPVRLPGSQEKAILGRCVVPLTEEVAVAPPEPEPVIAAVAAPPANYSSRGWLAPEQQQRIYRGNAS

γ-type Cas

Geobacillus_kaustophilus_GK2022 ------MLYPYNGKRPNVHETAFIAPGAYLIGDVTVGPESTIWFNAVLRGDEGPITIGARTSIQDNT

Geobacillus_thermodenitrificans_ ------MLYLYNGKKPNVHESVFIAPGARVIGDVTVGEESTIWFNAVLRGDEGPITIGARTSIQDNT

Bacillus_halodurans_BH0211 ------MIFRYKDFSPKVDTTAFIAESATVIGNVEIGAYTTVLFNAVIRGDEGLIKIGRRCNIQENV

Bacillus_subtilis_BG13896 ------MIYPYKEHKPDIHPTAFIADNATITGDVVIGEQSSIWFSVVIRGDVAPTRIGNRVNIQDLS

Bacillus_amyloliquefaciens_RBAM_ ------MIIYPYKQFTPDIHPEAFIADNAVITGDVVIGEQSSIWFSAVIRGDVAPTRIGKRVSIQDLS

Bacillus_licheniformis_DSM13_BLi ------MIYPYKKTEPVIHETAFIADNAVITGDVTIGERSSIWFSSVIRGDVAPVRIGKGVNIQDLS

Bacillus_licheniformis_ATCC_1458 ------MIYPYKKTEPVIHETAFIADNAVITGDVTIGERSSIWFSSVIRGDVAPVRIGKGVNIQDLS

Bacillus_pumilus_BPUM_2684 ------MIYPYHQFTPEIHESVFVADNATITGDVTIGEYSSVWFQTVIRGDVAPVRIGKNVNIQDLS

Bacillus_clausii_ABC2877 ------MILPYKGVVPTIDPSVYVAEGAIVTGDVTIGAQSSVWFHTVIRGDVAPTIIGERVNIQDQS

Bacillus_anthracis_Ames_0581_GBA ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_anthracis_Ames_BA5010 ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_anthracis_A2012_BA_5430 ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_anthracis_Sterne_BAS465 ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_cereus_ZK_BCZK4511 ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_thuringiensis_Al_Hakam_ ------MIYPYKEKNPKIASSAFIADYVTITGDVSIGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_cereus_ATCC_10987_BCE_4 ------MIYPYKEKNPKIASSAFIADYVTITGDVSVGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_thuringiensis_9727_BT97 ------MIYPYKEKNPKIASSAFIADYVTITGDVTVGEESSIWFNTVIRGDVSPTIIGNRVNVQDQC

Bacillus_cereus_ATCC_14579_BC475 ------MIYPYKEKKPKIASSAFIADYVTITGDVYVGEESSIWFNTVIRGDVSPTIIGDRVNVQDQC

Bacillus_cereus_cytotoxis_NVH_39 ------MIYSYKDKNPKIASSAFIADYVTITGDVTIGEESSIWFNTVIRGDVSPTIIGNRVNVQDQC

Bacillus_halodurans_BH3289 ------MLYPYKDKKPKIAENVFLADYVTITGDVTIGADSSIWYNTVIRGDVSPTFIGERVNIQDNS

Geobacillus_kaustophilus_GK2848 ------MIYPYKGKTPQIAASAFIADYVTITGDVVIGEETSIWFNTVIRGDVAPTVIGNRVNIQDNS

Geobacillus_thermodenitrificans_ ------MIYPYKGKTPQIAPSAFIADYVTITGDVTIGEETSIWFNTVIRGDVAPTIIGNRVNIQDNS

Oceanobacillus_iheyensis_OB2312 ------MIDHYNNIYPTIHPTAFVAKDAVINGDVQIKEKVSIWFHTVIRGDVSKTTIGKRTNIQDLC

Desulfotomaculum_reducens_Dred_0 ------MILPYLEYSPHIHPSVYIAPTATVVGHVEIHEHASIWYNAVIRGDVDRISIGKKTNIQDGC

Pelotomaculum_thermopropionicum_ ------MILPYDGVRPEIDETAFIAPTAVVVGRVEIGPYSSIWYNSVVRGDVDTVVIGACTSIQDGS

Caldicellulosiruptor_saccharolyt ------MIITYKGKTPKIAQSCYIAPNATIIGDVEIGENSSVWFGCVIRCEENRIVIGKNTNIQDLT

Alkaliphilus_oremlandii_Clos_171 ------MIKSFRDKNPMIHEHCFIAETANVIGDIVIGENSSIWYNVVIRADVNSVRIGKNTNIQDNS

Alkaliphilus_metalliredigens_Ame ------MIKDLGNKKTEIHESCFIAESADVIGKVKIGKNSSVWYKVVIRGDGNYIEIGENTNIQDNT

Clostridium_kluyveri_CKL_0987 ------MIRKFKYYMPELDESCFIADNAEVIGKVKLCEDVSIWFGAVLRGDLNHIYVGKGSNVQDNC

Clostridium_botulinum_A_Hall_CLC ------MIYEYNGKKPIIKKNVFLAKSADLIGDVVLEENCTVLFGAVLRGDINSIHIGNGSNVQDNC

Clostridium_botulinum_A_ATCC_193 ------MIYEYNGKKPIIKKNVFLAKSADLIGDVVLEENCTVLFGAVLRGDINSIHIGNGSNVQDNC

Clostridium_botulinum_A_CBO0530 ------MICEYNGKKPIIKKNVFLAKSADLIGDVVLEENCTVLFGAVLRGDINSIHIGNGSNVQDNC

Clostridium_botulinum_F_CLI_0609 ------MIYEYNGKKPIIKKNVFLAKSADLIGDVVLEENCTILFGAVLRGDINSIHIGNGSNVQDNC

Clostridium_acetobutylicum_CAC10 ------MIYSYDDKKPNIHSSVFIAKSADIIGDVNIDKNSSVWFGAVIRGDSNYIRIGEGTNIQDNS

Clostridium_difficile_CD2748 ------MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRGDEGPITIGENTNIQDCS

Clostridium_tetani_E88_CTC01682 ------MIRNFNNKTPKIHSNSFIESTSCIIGDVTIEDNVSIWFNAVLRADHNSIYVGKNSNIQDNC

Clostridium_beijerinckii_Cbei_10 MTISECNVKITLVFSEVIVMVKNYKDKKPVLDSDIYVSETAVIIGDVTLKKNANIWFGAVIRGDEASITIGENTNIQENC

Thermoanaerobacter_tengcongensis ------MIIKEYKGIKPQIDEEAYIAETAEIIGDVEIKKNVNIWYGAVLRGDVDKIVVEEGTNIQDNC

Clostridium_perfringens_ATCC_131 ------MLIEIRGKKPEIGEKTFIAHSSDIIGDVTIGRDCGIWFGSVIRGDDNLIKIGNETNVQDNA

Clostridium_perfringens_13_CPE16 ------MLIEIRGKKPEIGEKTFIAHSSDIIGDVTIGRDCGIWFGSVIRGDDNLIKIGNETNVQDNA

Clostridium_perfringens_SM101_CP ------MLIEIRGKKPEIGEKTFIAHSSDIIGDVTIGRDCGVWFGSVIRGDYNLIKIGNETNVQDNA

Desulfitobacterium_hafniense_DSY ------MLYSYLDKRPTLGKDVFIADGAKIIGDVHIDEGASIWYNSVIRGDIASIYIGKGTNIQDLS

Enterococcus_faecalis_EF2918 ------MKKEYFIAASADVYGNVQLAKDSNIWFQSVLRGDSNTITIGEGSNIQDGT

Synechococcus_elongatus_PCC7942_ ------MAAFGDRTWGEPDCQRATYVAESAVICGDVVLAEGVSIWPTAVLRGDVERIEIGCNSNVQDGA

Geobacillus_kaustophilus_GK2022 TCHLYEG-SPLVVEDEVTVGHNVILHGCTIRRRSIIGMGSTILDGAEIGEECIIGANTLIPSGKKIPPRSLVIGSPGKVV

Geobacillus_thermodenitrificans_ TCHLYEG-SPLVIEDEVTVGHNVVLHGCTIRRRSIIGMGSTILDGAEIGEECIIGANTLIPSGKKIPPRSLVVGSPGQVV

Bacillus_halodurans_BH0211 MCHLYEQ-YPLVLEDEVSLGHHAIVHGCTLKQGVLIGMGATVLDGAEIGEYSIVGAGSVVPPGKVIPPRSLVLGSPGKVV

Bacillus_subtilis_BG13896 CLHQSPN-KTLLIEDDATIGHQVTLHSAVIRKNALIGMGSIILDGAEIGEGAFIGAGSLVPPGKIIPPGHLAFGRPAKVI

Bacillus_amyloliquefaciens_RBAM_ CLHQSPN-RPLLIEDDVTIGHQVTLHSAIIKKNALIGMGSVILDGAEIGEGAFIGAGSLVPPGKTIPPGCLAFGRPAKVI

Bacillus_licheniformis_DSM13_BLi CLHQSPE-RPLVIEDGVTVGHQVTLHSSVIRKHALIGMGSIILDEAEIGEGAFIGAGSLVPPGKKIPSGHLAFGRPAKVI

Bacillus_licheniformis_ATCC_1458 CLHQSPE-RPLVIEDGVTVGHQVTLHSSVIRKHALIGMGSIILDEAEIGEGAFIGAGSLVPPGKKIPSGHLAFGRPAKVI

Bacillus_pumilus_BPUM_2684 CLHQSPG-KTLLIEDGATIGHQVTLHSSIIRKNALIGMGSIILDGAEIGEGAFIGAGSLVPQGKVIPKGSLAFGRPAKVV

Bacillus_clausii_ABC2877 MLHQSPH-SPLMIEDDVTIGHQAMLHSAIIRKQALIGMGSTILDGAEIGEGAFIGAGSLVPPGKVIPPGTLAFGRPAKVV

Bacillus_anthracis_Ames_0581_GBA TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_anthracis_Ames_BA5010 TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_anthracis_A2012_BA_5430 TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_anthracis_Sterne_BAS465 TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_cereus_ZK_BCZK4511 TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_thuringiensis_Al_Hakam_ TLHQSPQ-YPLILEDDVTIGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_cereus_ATCC_10987_BCE_4 TLHQSPQ-YPLILEDDVTVGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_thuringiensis_9727_BT97 TLHQSPQ-YPLILEDDVTVGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVV

Bacillus_cereus_ATCC_14579_BC475 TLHQSPQ-YPLILEDDVTVGHQVILHSCHIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPNTLAFGRPAKVI

Bacillus_cereus_cytotoxis_NVH_39 TLHQSPQ-YPLILEDDVTIGHQVILHSCMIKKDALIGMGSIILDGAEIGEGAFIGAGSLVSQGKKIPPHTLAFGRPAKVI

Bacillus_halodurans_BH3289 VLHQSPR-TPLVIEDDVTVGHQVILHSCTIRKEALIGMGSIILDGAEVGEGAFIGAGSLVPQGKKIPANSLAFGRPAKVV

Geobacillus_kaustophilus_GK2848 ILHQSPN-NPLIIEDGVTVGHQVILHSAIVRKNALIGMGSIILDRAEIGEGAFIGAGSLVPPGKKIPPNTLALGRPAKVV

Geobacillus_thermodenitrificans_ ILHQSPN-NPLIIEDGVTVGHQVILHSAIVRKHALIGMGSIILDRAEIGEGAFIGAGSLVPPGKKIPPNVLALGRPAKVV

Oceanobacillus_iheyensis_OB2312 MLHQSPG-QPLIIEEDVTVGHQVTLHSAIVRKKALIGMGSIVLDGAEIGEGAFLGAGSLVPPGKKIPAHTLAMGRPAKVV

Desulfotomaculum_reducens_Dred_0 MLHQDAG-FPLLIGENVTVGHHTILHGCTIGDRCLIGMGAIILNGAYIGSESLIGAGTLVKEGQEIPPGVLAVGSPARVV

Pelotomaculum_thermopropionicum_ ILHEHAG-FPLVIGDRVTVGHRVLLHGCTVEDGAYIGMGAIVLNGARIGAGAVVGAGSLVLQGQEIPPGMLALGSPARVV

Caldicellulosiruptor_saccharolyt TIHTDHC-CSVIIGDDVTIGHNVVLHGCDIGNNVLVGMGSIIMNGSKIGNNVLIGAGSLITQNTIIPPNTLVFGRPAKVI

Alkaliphilus_oremlandii_Clos_171 VIHN-SDDFPTIIGDDVTVGHNAIVHACSVGNKVLIGMGAIVLDGAEIGDETIIGAGSIVTSGKKIPSGVLALGSPAKVI

Alkaliphilus_metalliredigens_Ame VVHIDSEKYPTIIGDNVTVGHSAIVHACKVGNNALIGMGAIILDGSEIGDNTIIGAGSLVPPGKKIPSGVLAMGSPVKVV

Clostridium_kluyveri_CKL_0987 TIHTSVDKNPTEIGEYVTIGHNAIVHGGKIGNYSLIGMGSIILDNAEIGEETIIGAGSLVTQNKKIPSGVLCMGSPAKVI

Clostridium_botulinum_A_Hall_CLC ILHVDEGDLNIYIGDNVTVGHGAILHGCKINDNSLIGMGAIILNGAEIGSNTIIGAGSLITSNKKIPSGVLCMGSPAKVI

Clostridium_botulinum_A_ATCC_193 ILHVDEGDLNIYIGDNVTVGHGAILHGCKINDNSLIGMGAIILNGAEIGSNTIIGAGSLITSNKKIPSGVLCMGSPAKVI

Clostridium_botulinum_A_CBO0530 ILHVDEGDLNIYIGDNVTVGHGAILHGCKINDNSLIGMGAIILNGAEIGSNTIIGAGSLITSNKKIPSGVLCMGSPAKVI

Clostridium_botulinum_F_CLI_0609 ILHVDEGDLNIYIGNNVTVGHGAILHGCRINDNSLIGMGAIILNGAEIGSNTIIGAGSLITSNKKIPSGVLCMGSPAKVI

Clostridium_acetobutylicum_CAC10 VLHTNTYDNGIDIKNNVTIGHGVILHGCTINSNCIIGMGATILDDVEIGEYTIVGANSLITSGKKIPGGVLCMGSPAKVI

Clostridium_difficile_CD2748 IVHG---DTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILDNAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVV

Clostridium_tetani_E88_CTC01682 TLHIDTN-FKVKIGESVTVGHNSILHGCEIGNNTLIGMGSIILNGAKIGENAIVGAGTLIPQGKTFPSGVLLLGSPAKII

Clostridium_beijerinckii_Cbei_10 VVHVDYG-YNAVIGDCCTIGHGAIIHGCTIKNNVLVGMGSVILNGAKIGNNTIIGAGSLITQNKEFEDGVLILGNPAKVI

Thermoanaerobacter_tengcongensis VVHV-TDGHPCYIGKYCTIGHGAIVHACKVGNNVLIGMGAIILDDAEIGDNCIIGAGALVTGGKKIPPGSLVIGSPAKVV

Clostridium_perfringens_ATCC_131 VLHVDKE-HTIEIGSGVTIGHGAIIHGCKIEDECLIGMGAIILNGAKIGKNTMIAAGTLVSQNKEIPEGVLVMGVPGKVV

Clostridium_perfringens_13_CPE16 VLHVDKE-HTIEIGSGVTIGHGAIIHGCKIEDECLIGMGAIILNGAKIGKNTMIAAGTLVSQNKEIPEGVLVMGVPGKVV

Clostridium_perfringens_SM101_CP VLHGDKE-YKVEIGHGVTIGHGAIIHGCKIEDECLIGMGAIILNGAKIGKNTMIAAGTLVSQNKEIPEGVLVMGVPGKVV

Desulfitobacterium_hafniense_DSY VIHVNTN-VPVIIEDDVSIGHSVTLHGCTIKKGTMVGMGSTILNGAIIEEGSMVAAGSLITENKQFPPHVLIMGSPAKVV

Enterococcus_faecalis_EF2918 IIHVDED-APTIVGKYVTVGHQCMLHGCKIGDGALIGMGSTILNNAIIGENSLIGAGSLVTEGKIIPPNVLAFGRPAKVI

Synechococcus_elongatus_PCC7942_ VLHGDPG-QPTILEEDVTVGHRAVIHSANIGAGSLIGIGAIVLNGVQIGAGSIVGAGAVVTKS--IPAGSLAMGVPAKVV

Geobacillus_kaustophilus_GK2022 RELTDKDLELIQLSIDTYVQKGKEYREQLANHR------

Geobacillus_thermodenitrificans_ RELTDKDLALIQLSIDTYVQKGKEYRKQLTAAESTDKETSKQV-

Bacillus_halodurans_BH0211 REVTEQDLAMIEETVETYVRNGQEFKRSDVLERIR------

Bacillus_subtilis_BG13896 RPLTEEDRKDMQRIRSEYVEKGQYYKFLQQT------

Bacillus_amyloliquefaciens_RBAM_ RRLTEEDIRDMERIRTEYVEKGRYYKTLQEK------

Bacillus_licheniformis_DSM13_BLi RPLTDKDKQEMERIRKEYIEKGQYYKSLQKKD------

Bacillus_licheniformis_ATCC_1458 RPLTDKDKQEMERIRKEYIEKGQYYKSLQKKD------

Bacillus_pumilus_BPUM_2684 RQLTDEDIQDMDRIRREYVEKGQYYRSLISR------

Bacillus_clausii_ABC2877 RPLNETDKKEMARIRREYVEKGQYYKSLQQQTATRNKDQQ----

Bacillus_anthracis_Ames_0581_GBA RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_anthracis_Ames_BA5010 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_anthracis_A2012_BA_5430 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_anthracis_Sterne_BAS465 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_cereus_ZK_BCZK4511 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_thuringiensis_Al_Hakam_ RKLTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_cereus_ATCC_10987_BCE_4 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_thuringiensis_9727_BT97 RELTEEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_cereus_ATCC_14579_BC475 RELTAEDRKDMERIRTQYVEKGQYYKSLQK------

Bacillus_cereus_cytotoxis_NVH_39 RELTDADRKDMERIRKQYVEKGQYYKSLQK------

Bacillus_halodurans_BH3289 RTLTEEDRKDMARIRREYVEKGQYYKQVQQEYRS------

Geobacillus_kaustophilus_GK2848 RELTEDDIREMERIRREYVEKGQYYKALQQQRTSCADKKELP--

Geobacillus_thermodenitrificans_ RELTEDDFREMERIRREYVEKGQYYKALQQNRS------

Oceanobacillus_iheyensis_OB2312 RELTEEDYKEMDRVRNSYVEKGQYYKNNTNMGI------

Desulfotomaculum_reducens_Dred_0 RKLTEEEKQKLSQSAQHYFNMAEKHSISQK------

Pelotomaculum_thermopropionicum_ RPIREDEVDRFLGAVGRYLKMAEKHARTAAGKAR------

Caldicellulosiruptor_saccharolyt RELTQEEIERIRISAKEYIELSNDYKNICK------

Alkaliphilus_oremlandii_Clos_171 RELTVEEKKSLIDSAEKYVKLSKDHKVE------

Alkaliphilus_metalliredigens_Ame RELTEDEKNGLKKSAEEYVKYGKEHKNNQ------

Clostridium_kluyveri_CKL_0987 RKLTIEEKKFLRHSAEEYIRQSKSYKQYIIV------

Clostridium_botulinum_A_Hall_CLC RELNEEEIASIKESAEDYLTLNKNFNRE------

Clostridium_botulinum_A_ATCC_193 RELNEEEIASIKESAEDYLTLNKNFNRE------

Clostridium_botulinum_A_CBO0530 RELNEEEIASIKESAEDYLTLNKNFNRE------

Clostridium_botulinum_F_CLI_0609 RKLNEEEIASIKESAEDYLTLNKNFNRE------

Clostridium_acetobutylicum_CAC10 RELTVDEKLEIDKNAEHYIEMG--KKYFK------

Clostridium_difficile_CD2748 RELTEEDKKYIDESYEWYLEAAQNQKY------

Clostridium_tetani_E88_CTC01682 RNLTSEEIENIKKSSDEYVKLSKTY------

Clostridium_beijerinckii_Cbei_10 RKLTQEEIEGIKKSYISYLELSKDFK------

Thermoanaerobacter_tengcongensis RQLTEEEIESIHKSYEHYVELAKLHFSEFGQLTVYNKSNIIENS

Clostridium_perfringens_ATCC_131 RKLTEDEIESIKNSRREYVKMKNLYIK------

Clostridium_perfringens_13_CPE16 RKLTEDEIESIKNSRREYVKMKNLYIK------

Clostridium_perfringens_SM101_CP RKLTEDEIESIKNSRREYVKMKNLYIK------

Desulfitobacterium_hafniense_DSY RELTSDEVNALKETAERYSQKAREHRENSPLPIAKC------

Enterococcus_faecalis_EF2918 RPLTAAEIASNHTNALHYIQRAEEFRQQKYPKLT------

Synechococcus_elongatus_PCC7942_ RSLSAAEIADLRQHAQHYVALAEAHAQQQAGKTSEKRSESAQ—